LOCUS       RC224818                7506 bp    DNA     circular  SYN 
DEFINITION  ENPP3 (Myc-DDK-tagged)-Human ectonucleotide pyrophosphatase/phosphodiesterase 3 (ENPP3)
ACCESSION   NM_005021
VERSION     NM_005021.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3663..3754 
                     /label="Myc-DDK"  
     misc_feature    3784..4369 
                     /label="PolyA signal"  
     misc_feature    3841..3860 
                     /label="Restriction Enzyme"  
     misc_feature    4645..5317 
                     /label="ColE1"  
     misc_feature    5747..6541 
                     /label="Kan/Neo"  
     misc_feature    6576..6878 
                     /label="SV40 ori"  
     misc_feature    6964..7441 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3645..3651 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3645 
                     /label="ENPP3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAATCTACG TTGACTTTAG CAACGGAACA ACCTGTTAAG AAGAACACTC 
     1081 TTAAGAAATA TAAAATAGCT TGCATTGTTC TTCTTGCTTT GCTGGTGATC ATGTCACTTG 
     1141 GATTAGGCCT GGGGCTTGGA CTCAGGAAAC TGGAAAAGCA AGGCAGCTGC AGGAAGAAGT 
     1201 GCTTTGATGC ATCATTTAGA GGACTGGAGA ACTGCCGGTG TGATGTGGCA TGTAAAGACC 
     1261 GAGGTGATTG CTGCTGGGAT TTTGAAGACA CCTGTGTGGA ATCAACTCGA ATATGGATGT 
     1321 GCAATAAATT TCGTTGTGGA GAGACCAGAT TAGAGGCCAG CCTTTGCTCT TGTTCAGATG 
     1381 ACTGTTTGCA GAGGAAAGAT TGCTGTGCTG ACTATAAGAG TGTTTGCCAA GGAGAAACCT 
     1441 CATGGCTGGA AGAAAACTGT GACACAGCCC AGCAGTCTCA GTGCCCAGAA GGGTTTGACC 
     1501 TGCCACCAGT TATCTTGTTT TCTATGGATG GATTTAGAGC TGAATATTTA TACACATGGG 
     1561 ATACTTTAAA GCCAAATATC AATAAACTGA AAACATGTGG AATTCATTCA AAATACATGA 
     1621 GAGCTATGTA TCCTACCAAA ACCTTCCCAA ATCATTACAC CATTGTCACG GGCTTGTATC 
     1681 CAGAGTCACA TGGCATCATT GACAATAATA TGTATGATGT AAATCTCAAC AAGAATTTTT 
     1741 CACTTTCTTC AAAGGAACAA AATAATCCAG CCTGGTGGCA TGGGCAACCA ATGTGGCTGA 
     1801 CAGCAATGTA TCAAGGTTTA AAAGCCGCTA CCTACTTTTG GCCCGGATCA GAAGTGGCTA 
     1861 TAAATGGCTC CTTTCCTTCC ATATACATGC CTTACAACGG AAGTGTCCCA TTTGAAGAGA 
     1921 GGATTTCTAC ACTGTTAAAA TGGCTGGACC TGCCCAAAGC TGAAAGACCC AGGTTTTATA 
     1981 CCATGTATTT TGAAGAACCT GATTCCTCTG GACATGCAGG TGGACCAGTC AGTGCCAGAG 
     2041 TAATTAAAGC CTTACAGGTA GTAGATCATG CTTTTGGGAT GTTGATGGAA GGCCTGAAGC 
     2101 AGCGGAATTT GCACAACTGT GTCAATATCA TCCTTCTGGC TGACCATGGA ATGGACCAGA 
     2161 CTTATTGTAA CAAGATGGAA TACATGACTG ATTATTTTCC CAGAATAAAC TTCTTCTACA 
     2221 TGTACGAAGG GCCTGCCCCC CGCATCCGAG CTCATAATAT ACCTCATGAC TTTTTTAGTT 
     2281 TTAATTCTGA GGAAATTGTT AGAAACCTCA GTTGCCGAAA ACCTGATCAG CATTTCAAGC 
     2341 CCTATTTGAC TCCTGATTTG CCAAAGCGAC TGCACTATGC CAAGAACGTC AGAATCGACA 
     2401 AAGTTCATCT CTTTGTGGAT CAACAGTGGC TGGCTGTTAG GAGTAAATCA AATACAAATT 
     2461 GTGGAGGAGG CAACCATGGT TATAACAATG AGTTTAGGAG CATGGAGGCT ATCTTTCTGG 
     2521 CACATGGACC CAGTTTTAAA GAGAAGACTG AAGTTGAACC ATTTGAAAAT ATTGAAGTCT 
     2581 ATAACCTAAT GTGTGATCTT CTACGCATTC AACCAGCACC AAACAATGGA ACCCATGGTA 
     2641 GTTTAAACCA TCTTCTGAAG GTGCCTTTTT ATGAGCCATC CCATGCAGAG GAGGTGTCAA 
     2701 AGTTTTCTGT TTGTGGCTTT GCTAATCCAT TGCCCACAGA GTCTCTTGAC TGTTTCTGCC 
     2761 CTCACCTACA AAATAGTACT CAGCTGGAAC AAGTGAATCA GATGCTAAAT CTCACCCAAG 
     2821 AAGAAATAAC AGCAACAGTG AAAGTAAATT TGCCATTTGG GAGGCCTAGG GTACTGCAGA 
     2881 AGAACGTGGA CCACTGTCTC CTTTACCACA GGGAATATGT CAGTGGATTT GGAAAAGCTA 
     2941 TGAGGATGCC CATGTGGAGT TCATACACAG TCCCCCAGTT GGGAGACACA TCGCCTCTGC 
     3001 CTCCCACTGT CCCAGACTGT CTGCGGGCTG ATGTCAGGGT TCCTCCTTCT GAGAGCCAAA 
     3061 AATGTTCCTT CTATTTAGCA GACAAGAATA TCACCCACGG CTTCCTCTAT CCTCCTGCCA 
     3121 GCAATAGAAC ATCAGATAGC CAATATGATG CTTTAATTAC TAGCAATTTG GTACCTATGT 
     3181 ATGAAGAATT CAGAAAAATG TGGGACTACT TCCACAGTGT TCTTCTTATA AAACATGCCA 
     3241 CAGAAAGAAA TGGAGTAAAT GTGGTTAGTG GACCAATATT TGATTATAAT TATGATGGCC 
     3301 ATTTTGATGC TCCAGATGAA ATTACCAAAC ATTTAGCCAA CACTGATGTT CCCATCCCAA 
     3361 CACACTACTT TGTGGTGCTG ACCAGTTGTA AAAACAAGAG CCACACACCG GAAAACTGCC 
     3421 CTGGGTGGCT GGATGTCCTA CCCTTTATCA TCCCTCACCG ACCTACCAAC GTGGAGAGCT 
     3481 GTCCTGAAGG TAAACCAGAA GCTCTTTGGG TTGAAGAAAG ATTTACAGCT CACATTGCCC 
     3541 GGGTCCGTGA TGTAGAACTT CTCACTGGGC TTGACTTCTA TCAGGATAAA GTGCAGCCTG 
     3601 TCTCTGAAAT TTTGCAACTA AAGACATATT TACCAACATT TGAAACCACT ATTACGCGTA 
     3661 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     3721 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     3781 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     3841 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     3901 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     3961 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     4021 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     4081 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     4141 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     4201 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     4261 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     4321 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     4381 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     4441 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     4501 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     4561 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     4621 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     4681 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     4741 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     4801 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     4861 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     4921 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     4981 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     5041 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     5101 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     5161 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     5221 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     5281 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     5341 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     5401 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     5461 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     5521 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     5581 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     5641 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     5701 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     5761 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     5821 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     5881 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     5941 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     6001 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     6061 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     6121 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     6181 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     6241 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     6301 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     6361 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     6421 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     6481 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     6541 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     6601 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     6661 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     6721 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     6781 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     6841 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     6901 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     6961 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     7021 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     7081 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     7141 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     7201 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     7261 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     7321 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     7381 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     7441 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     7501 AATTTT
//