LOCUS       RC224793                9030 bp    DNA     circular  SYN 
DEFINITION  TMEM2 (Myc-DDK-tagged)-Human transmembrane protein 2 (TMEM2), transcript variant 1
ACCESSION   NM_013390
VERSION     NM_013390.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5187..5278 
                     /label="Myc-DDK"  
     misc_feature    5308..5893 
                     /label="PolyA signal"  
     misc_feature    5365..5384 
                     /label="Restriction Enzyme"  
     misc_feature    6169..6841 
                     /label="ColE1"  
     misc_feature    7271..8065 
                     /label="Kan/Neo"  
     misc_feature    8100..8402 
                     /label="SV40 ori"  
     misc_feature    8488..8965 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5169..5175 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5169 
                     /label="TMEM2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTATGCCACT GATTCCAGGG GACACTCCCC TGCTTTCCTC CAACCTCAGA 
     1081 ATGGAAATAG TCGTCACCCA TCTGGCTATG TTCCAGGGAA GGTTGTCCCA TTGCGTCCCC 
     1141 CTCCTCCTCC AAAGAGTCAA GCTTCAGCCA AATTTACCTC CATCAGACGA GAAGACCGGG 
     1201 CAACCTTCGC ATTCTCACCT GAAGAACAGC AAGCCCAGAG AGAAAGTCAA AAGCAAAAGA 
     1261 GACACAAAAA TACTTTCATT TGTTTTGCTA TTACTAGTTT CTCATTTTTT ATTGCACTTG 
     1321 CAATCATTTT AGGAATATCC TCAAAATATG CTCCAGATGA AAATTGCCCA GATCAAAATC 
     1381 CTCGTCTCAG GAATTGGGAT CCAGGACAAG ATTCTGCAAA GCAAGTTGTT ATCAAGGAGG 
     1441 GAGATATGCT CCGTCTGACC TCAGACGCCA CCGTGCATTC TATAGTCATT CAGGATGGAG 
     1501 GACTGCTTGT ATTTGGGGAC AATAAAGATG GATCCAGAAA TATTACTTTG AGGACTCATT 
     1561 ACATCCTGAT CCAGGATGGT GGGGCGCTTC ATATTGGAGC AGAAAAATGC CGCTATAAAT 
     1621 CCAAAGCGAC AATTACCTTG TATGGCAAGT CAGATGAAGG TGAAAGTATG CCAACATTTG 
     1681 GCAAAAAGTT TATTGGTGTG GAAGCTGGCG GGACACTGGA GTTACATGGG GCACGGAAGG 
     1741 CATCGTGGAC GTTGTTGGCA AGGACCCTGA ATTCCTCAGG CTTGCCCTTT GGGTCCTATA 
     1801 CCTTTGAAAA GGACTTTTCC CGGGGCCTCA ATGTGAGGGT CATTGACCAA GACACGGCCA 
     1861 AAATTTTGGA AAGTGAGAGA TTTGATACCC ATGAATACCG CAATGAGAGC AGGCGGCTTC 
     1921 AGGAGTTTCT GAGATTCCAG GATCCAGGTC GGATTGTTGC CATAGCTGTC GGGGATTCAG 
     1981 CCGCTAAAAG TCTCTTACAA GGAACCATCC AGATGATCCA GGAACGGTTG GGAAGTGAAC 
     2041 TGATCCAAGG ACTGGGCTAC AGGCAAGCTT GGGCTTTAGT TGGTGTCATT GATGGTGGAA 
     2101 GCACTTCTTG CAATGAATCC GTGAGAAACT ATGAAAATCA TAGCAGTGGC GGGAAGGCTC 
     2161 TTGCCCAAAG AGAATTTTAT ACTGTGGATG GCCAGAAGTT CTCTGTGACA GCTTATAGTG 
     2221 AATGGATTGA AGGCGTTTCT CTTTCAGGAT TCCGGGTAGA GGTTGTAGAT GGAGTGAAGC 
     2281 TAAATTTGCT AGATGATGTT AGTAGTTGGA AACCTGGAGA CCAGATTGTG GTCGCAAGCA 
     2341 CAGACTATTC CATGTACCAA GCAGAGGAGT TCACTCTTCT TCCCTGTTCT GAATGCAGCC 
     2401 ATTTTCAGGT CAAAGTCAAA GAAACCCCTC AGTTCCTGCA CATGGGTGAG ATCATAGACG 
     2461 GTGTAGACAT GAGAGCTGAG GTTGGAATTC TTACCCGGAA TATTGTGATC CAAGGAGAAG 
     2521 TGGAGGACTC ATGCTACGCA GAAAATCAGT GCCAATTTTT TGATTATGAT ACCTTTGGGG 
     2581 GACACATTAT GATAATGAAA AATTTTACTT CAGTCCATCT TTCTTATGTG GAATTGAAAC 
     2641 ACATGGGTCA GCAGCAGATG GGGCGATACC CTGTTCATTT TCACCTGTGT GGTGACGTGG 
     2701 ATTATAAAGG AGGATACAGA CATGCAACAT TTGTGGACGG CCTGTCTATT CATCACAGCT 
     2761 TCTCAAGGTG CATCACTGTG CATGGGACAA ATGGCTTGCT AATAAAAGAC ACCATTGGGT 
     2821 TTGACACACT AGGTCATTGT TTCTTTTTGG AAGATGGTAT TGAACAGAGG AATACTTTGT 
     2881 TCCACAATCT GGGACTCCTC ACCAAGCCGG GTACTCTCCT GCCCACCGAT AGGAACAACT 
     2941 CCATGTGTAC CACCATGCGA GATAAAGTGT TTGGAAATTA CATTCCTGTG CCTGCTACTG 
     3001 ACTGTATGGC TGTTTCAACT TTCTGGATTG CTCATCCCAA CAATAATCTG ATTAATAATG 
     3061 CAGCTGCAGG CTCACAGGAT GCTGGAATAT GGTATTTATT CCACAAGGAA CCAACTGGGG 
     3121 AATCCAGTGG ATTGCAGCTC TTGGCAAAAC CAGAACTCAC TCCATTGGGT ATATTTTATA 
     3181 ACAACAGGGT CCATTCAAAT TTTAAGGCTG GCTTATTTAT TGACAAAGGT GTCAAAACAA 
     3241 CCAACTCTAG TGCTGCTGAC CCAAGGGAAT ACCTCTGTTT GGACAATAGT GCAAGATTTC 
     3301 GACCTCATCA GGATGCAAAC CCCGAAAAAC CACGTGTTGC TGCTCTAATT GACAGGCTCA 
     3361 TTGCTTTTAA AAATAATGAT AATGGAGCTT GGGTCAGAGG AGGAGATATT ATCGTTCAAA 
     3421 ATTCAGCATT TGCAGATAAT GGAATAGGAC TGACCTTTGC CAGTGATGGA AGCTTCCCAA 
     3481 GTGATGAAGG TTCCAGCCAA GAGGTATCTG AATCTCTCTT TGTTGGGGAG AGCAGGAATT 
     3541 ACGGCTTTCA GGGTGGTCAG AACAAGTATG TAGGCACTGG AGGAATAGAC CAGAAGCCTC 
     3601 GAACATTACC CAGGAACAGG ACGTTCCCAA TTAGAGGCTT TCAGATTTAT GATGGGCCCA 
     3661 TTCATCTCAC AAGGAGCACT TTCAAAAAAT ATGTGCCAAC TCCAGATAGG TACAGCAGTG 
     3721 CAATTGGCTT CCTCATGAAG AATTCCTGGC AGATAACCCC CAGGAATAAT ATCTCCCTCG 
     3781 TGAAGTTTGG TCCACATGTC TCTCTGAATG TCTTTTTTGG AAAGCCTGGT CCCTGGTTTG 
     3841 AAGATTGTGA GATGGATGGT GATAAGAACT CCATATTCCA TGACATTGAT GGCTCTGTGA 
     3901 CAGGATACAA GGATGCTTAT GTGGGAAGAA TGGACAACTA CCTGATCCGC CATCCAAGCT 
     3961 GTGTAAATGT GTCTAAGTGG AATGCAGTGA TCTGCAGTGG GACCTATGCA CAGGTCTATG 
     4021 TACAGACATG GAGCACTCAG AATCTTTCTA TGACCATTAC ACGAGATGAG TATCCGTCCA 
     4081 ACCCTATGGT GCTCCGAGGT ATTAATCAGA AGGCTGCCTT TCCACAGTAC CAGCCTGTCG 
     4141 TCATGCTGGA GAAGGGTTAT ACCATCCACT GGAATGGGCC GGCACCACGG ACTACATTTC 
     4201 TATACCTCGT CAACTTCAAC AAGAATGACT GGATTCGAGT TGGCCTTTGC TATCCATCAA 
     4261 ACACAAGTTT TCAAGTTACC TTTGGCTATT TGCAGCGGCA GAATGGCTCA TTATCCAAAA 
     4321 TCGAAGAATA TGAGCCTGTG CATTCACTGG AAGAACTGCA AAGAAAGCAA TCCGAGAGGA 
     4381 AATTCTATTT TGACTCCAGC ACGGGGTTAC TGTTTTTGTA TCTCAAAGCC AAAAGCCACA 
     4441 GGCATGGCCA CAGTTACTGT TCATCTCAGG GATGTGAAAG AGTCAAGATC CAAGCAGCCA 
     4501 CAGACTCAAA GGACATCAGT AACTGCATGG CCAAAGCATA CCCACAGTAC TACAGAAAGC 
     4561 CGTCAGTGGT CAAGCGGATG CCGGCCATGC TCACTGGACT CTGTCAAGGC TGTGGCACTC 
     4621 GGCAGGTGGT GTTTACTAGT GATCCTCATA AAAGTTACCT CCCTGTGCAA TTCCAGTCAC 
     4681 CTGATAAAGC AGAAACCCAG CGTGGAGACC CGTCTGTTAT TTCTGTCAAT GGCACTGACT 
     4741 TTACCTTCCG AAGTGCAGGC GTCCTCCTCC TTGTTGTGGA TCCGTGCAGC GTTCCATTCC 
     4801 GCTTGACGGA AAAAACGGTT TTTCCTCTTG CTGATGTCAG TCGCATTGAA GAGTATTTAA 
     4861 AAACAGGCAT CCCTCCAAGG TCCATTGTTC TGTTGAGCAC AAGAGGAGAA ATAAAGCAGT 
     4921 TAAACATTTC ACACTTACTA GTACCTCTGG GATTAGCCAA ACCAGCTCAT CTTTATGACA 
     4981 AAGGGAGTAC CATATTTTTG GGATTCAGTG GAAACTTTAA ACCATCATGG ACTAAGCTAT 
     5041 TTACCAGTCC TGCTGGACAG GGCCTTGGGG TGCTTGAACA ATTCATACCT TTGCAGCTGG 
     5101 ACGAATATGG TTGTCCCAGA GCCACCACTG TCCGCAGAAG AGACCTGGAA CTGCTAAAGC 
     5161 AAGCTTCAAA AGCACATACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     5221 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     5281 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     5341 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     5401 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     5461 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     5521 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     5581 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     5641 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     5701 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     5761 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     5821 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     5881 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     5941 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     6001 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     6061 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     6121 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     6181 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     6241 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     6301 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     6361 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     6421 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     6481 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     6541 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     6601 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     6661 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     6721 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     6781 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     6841 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     6901 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     6961 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     7021 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     7081 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     7141 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     7201 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     7261 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     7321 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     7381 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     7441 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     7501 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     7561 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     7621 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     7681 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     7741 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     7801 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     7861 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     7921 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     7981 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     8041 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     8101 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     8161 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     8221 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     8281 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     8341 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     8401 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     8461 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     8521 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     8581 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     8641 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     8701 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     8761 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     8821 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     8881 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     8941 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     9001 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//