LOCUS       RC224777                8751 bp    DNA     circular  SYN 
DEFINITION  PER1 (Myc-DDK-tagged)-Human period homolog 1 (Drosophila) (PER1)
ACCESSION   NM_002616
VERSION     NM_002616.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4908..4999 
                     /label="Myc-DDK"  
     misc_feature    5029..5614 
                     /label="PolyA signal"  
     misc_feature    5086..5105 
                     /label="Restriction Enzyme"  
     misc_feature    5890..6562 
                     /label="ColE1"  
     misc_feature    6992..7786 
                     /label="Kan/Neo"  
     misc_feature    7821..8123 
                     /label="SV40 ori"  
     misc_feature    8209..8686 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4890..4896 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4890 
                     /label="PER1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGTGGCCCC CTAGAAGGGG CTGATGGGGG AGGGGACCCC AGGCCTGGGG 
     1081 AATCATTTTG TCCTGGGGGC GTCCCATCCC CTGGGCCCCC ACAGCACCGG CCTTGCCCAG 
     1141 GCCCCAGCCT GGCCGATGAC ACCGATGCCA ACAGCAATGG TTCAAGTGGC AATGAGTCCA 
     1201 ACGGGCATGA GTCTAGAGGC GCATCTCAGC GGAGCTCACA CAGCTCCTCC TCAGGCAACG 
     1261 GCAAGGACTC AGCCCTGCTG GAGACCACTG AGAGCAGCAA GAGCACAAAC TCTCAGAGCC 
     1321 CATCCCCACC CAGCAGTTCC ATTGCCTACA GCCTCCTGAG TGCCAGCTCA GAGCAGGACA 
     1381 ACCCGTCCAC CAGTGGCTGC AGCAGTGAAC AGTCAGCCCG GGCAAGGACT CAGAAGGAAC 
     1441 TCATGACAGC ACTTCGAGAG CTCAAGCTTC GACTGCCGCC AGAGCGCCGG GGCAAGGGCC 
     1501 GCTCTGGGAC CCTGGCCACG CTGCAGTACG CACTGGCCTG TGTCAAGCAG GTGCAGGCCA 
     1561 ACCAGGAATA CTACCAGCAG TGGAGCCTGG AGGAGGGCGA GCCTTGCTCC ATGGACATGT 
     1621 CCACCTATAC CCTGGAGGAG CTGGAGCACA TCACGTCTGA GTACACACTT CAGAACCAGG 
     1681 ATACCTTCTC AGTGGCTGTC TCCTTCCTGA CGGGCCGAAT CGTCTACATT TCGGAGCAGG 
     1741 CAGCCGTCCT GCTGCGTTGC AAGCGGGACG TGTTCCGGGG TACCCGCTTC TCTGAGCTCC 
     1801 TGGCTCCCCA GGATGTGGGA GTCTTCTATG GTTCCACTGC TCCATCTCGC CTGCCCACCT 
     1861 GGGGCACAGG GGCCTCAGCA GGTTCAGGCC TCAGGGACTT TACCCAGGAG AAGTCCGTCT 
     1921 TCTGCCGTAT CAGAGGAGGT CCTGACCGGG ATCCAGGGCC TCGGTACCAG CCATTCCGCC 
     1981 TAACCCCGTA TGTGACCAAG ATCCGGGTCT CAGATGGGGC CCCTGCACAG CCGTGCTGCC 
     2041 TGCTGATTGC AGAGCGCATC CATTCGGGTT ACGAAGCTCC CCGGATACCC CCTGACAAGA 
     2101 GGATTTTCAC TACGCGGCAC ACACCCAGCT GCCTCTTCCA GGATGTGGAT GAAAGGGCTG 
     2161 CCCCCCTGCT GGGCTACCTG CCCCAGGACC TCCTGGGGGC CCCAGTGCTC CTGTTCCTGC 
     2221 ATCCTGAGGA CCGACCCCTC ATGCTGGCTA TCCACAAGAA GATTCTGCAG TTGGCGGGCC 
     2281 AGCCCTTTGA CCACTCCCCT ATCCGCTTCT GTGCCCGCAA CGGGGAGTAT GTCACCATGG 
     2341 ACACCAGCTG GGCTGGCTTT GTGCACCCCT GGAGCCGCAA GGTAGCCTTC GTGTTGGGCC 
     2401 GCCACAAAGT ACGCACGGCC CCCCTGAATG AGGACGTGTT CACTCCCCCG GCCCCCAGCC 
     2461 CAGCTCCCTC CCTGGACACT GATATCCAGG AGCTGTCAGA GCAGATCCAC CGGCTGCTGC 
     2521 TGCAGCCCGT CCACAGCCCC AGCCCCACGG GACTCTGTGG AGTCGGCGCC GTGACATCCC 
     2581 CAGGCCCTCT CCACAGCCCT GGGTCCTCCA GTGATAGCAA CGGGGGTGAT GCAGAGGGGC 
     2641 CTGGGCCTCC TGCGCCAGTG ACTTTCCAAC AGATCTGTAA GGATGTGCAT CTGGTGAAGC 
     2701 ACCAGGGCCA GCAGCTTTTT ATTGAGTCTC GGGCCCGGCC TCAGTCCCGG CCCCGCCTCC 
     2761 CTGCTACAGG CACGTTCAAG GCCAAGGCCC TTCCCTGCCA ATCCCCAGAC CCAGAGCTGG 
     2821 AGGCGGGTTC TGCTCCCGTC CAGGCCCCAC TAGCCTTGGT CCCTGAGGAG GCCGAGAGGA 
     2881 AAGAAGCCTC CAGCTGCTCC TACCAGCAGA TCAACTGCCT GGACAGCATC CTCAGGTACC 
     2941 TGGAGAGCTG CAACCTCCCC AGCACCACTA AGCGTAAATG TGCCTCCTCC TCCTCCTATA 
     3001 CCACCTCCTC AGCCTCTGAC GACGACAGGC AGAGGACAGG TCCAGTCTCT GTGGGGACCA 
     3061 AGAAAGATCC GCCGTCAGCA GCGCTGTCTG GGGAGGGGGC CACCCCACGG AAGGAGCCAG 
     3121 TGGTGGGAGG CACCCTGAGC CCGCTCGCCC TGGCCAATAA GGCGGAGAGT GTGGTGTCCG 
     3181 TCACCAGTCA GTGTAGCTTC AGCTCCACCA TCGTCCATGT GGGAGACAAG AAGCCCCCGG 
     3241 AGTCGGACAT CATCATGATG GAGGACCTGC CTGGTCTAGC CCCAGGCCCA GCCCCCAGCC 
     3301 CAGCCCCCAG CCCCACAGTA GCCCCTGACC CAGCCCCAGA CGCCTACCGT CCAGTGGGGC 
     3361 TGACCAAGGC CGTGCTGTCC CTGCACACGC AGAAGGAAGA GCAAGCCTTC CTCAGCCGCT 
     3421 TCCGAGACCT GGGCAGGCTG CGTGGACTCG ACAGCTCTTC CACAGCTCCC TCAGCCCTTG 
     3481 GCGAGCGAGG CTGCCACCAC GGCCCCGCAC CCCCAAGCCG CCGACACCAC TGCCGATCCA 
     3541 AAGCCAAGCG CTCACGCCAC CACCAGAACC CTCGGGCTGA AGCGCCCTGC TATGTCTCAC 
     3601 ACCCCTCACC CGTGCCACCC TCCACCCCCT GGCCCACCCC ACCAGCCACT ACCCCCTTCC 
     3661 CAGCGGTTGT CCAGCCCTAC CCTCTCCCAG TGTTCTCTCC TCGAGGAGGC CCCCAGCCTC 
     3721 TTCCCCCTGC TCCCACATCT GTGCCCCCAG CTGCTTTCCC CGCCCCTTTG GTGACCCCAA 
     3781 TGGTGGCCTT GGTGCTCCCT AACTATCTGT TCCCAACCCC ATCCAGCTAT CCTTATGGGG 
     3841 CACTCCAGAC CCCTGCTGAA GGGCCTCCCA CTCCTGCCTC GCACTCCCCT TCTCCATCCT 
     3901 TGCCCGCCCT CCCCCCGAGT CCTCCTCACC GCCCGGACTC TCCACTGTTC AACTCGAGAT 
     3961 GCAGCTCTCC ACTCCAGCTC AATCTGCTGC AGCTGGAGGA GCTCCCCCGT GCTGAGGGGG 
     4021 CTGCTGTTGC AGGAGGCCCT GGGAGCAGTG CCGGGCCCCC ACCTCCCAGT GCGGAGGCTG 
     4081 CTGAGCCAGA GGCCAGACTG GCGGAGGTCA CTGAGTCCTC CAATCAGGAC GCACTTTCCG 
     4141 GCTCCAGTGA CCTGCTCGAA CTTCTGCTGC AAGAGGACTC GCGCTCCGGC ACAGGCTCCG 
     4201 CAGCCTCGGG CTCCTTGGGC TCTGGCTTGG GCTCTGGGTC TGGTTCAGGC TCCCATGAAG 
     4261 GGGGCAGCAC CTCAGCCAGC ATCACTCGCA GCAGCCAGAG CAGCCACACA AGCAAATACT 
     4321 TTGGCAGCAT CGACTCTTCC GAGGCTGAGG CTGGGGCTGC TCGGGGCGGG GCTGAGCCTG 
     4381 GGGACCAGGT GATTAAGTAC GTGCTCCAGG ATCCCATTTG GCTGCTCATG GCCAATGCTG 
     4441 ACCAGCGCGT CATGATGACC TACCAGGTGC CCTCCAGGGA CATGACCTCT GTGCTGAAGC 
     4501 AGGATCGGGA GCGGCTCCGA GCCATGCAGA AGCAGCAGCC TCGGTTTTCT GAGGACCAGC 
     4561 GGCGGGAACT GGGTGCTGTG CACTCCTGGG TCCGGAAGGG CCAACTGCCT CGGGCTCTTG 
     4621 ATGTGATGGC CTGTGTGGAC TGTGGGAGCA GCACCCAAGA TCCTGGTCAC CCTGATGACC 
     4681 CACTCTTCTC AGAGCTGGAT GGACTGGGGC TGGAGCCCAT GGAAGAGGGT GGAGGCGAGC 
     4741 AGGGCAGCAG CGGTGGCGGC AGTGGTGAGG GAGAGGGCTG CGAGGAGGCC CAAGGCGGGG 
     4801 CCAAGGCTTC AAGCTCTCAG GACTTGGCTA TGGAGGAGGA GGAAGAAGGC AGGAGCTCAT 
     4861 CCAGTCCAGC CTTACCTACA GCAGGAAACT GCACCAGCAC GCGTACGCGG CCGCTCGAGC 
     4921 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     4981 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     5041 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     5101 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     5161 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     5221 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     5281 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     5341 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     5401 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     5461 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     5521 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     5581 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     5641 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     5701 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     5761 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     5821 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     5881 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     5941 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     6001 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     6061 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     6121 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     6181 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     6241 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     6301 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     6361 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     6421 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     6481 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     6541 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     6601 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     6661 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     6721 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     6781 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     6841 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     6901 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     6961 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     7021 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     7081 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     7141 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     7201 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     7261 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     7321 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     7381 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     7441 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     7501 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     7561 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     7621 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     7681 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     7741 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     7801 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     7861 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     7921 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     7981 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     8041 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     8101 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     8161 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     8221 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     8281 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     8341 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     8401 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     8461 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     8521 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     8581 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     8641 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     8701 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
//