LOCUS       RC224695                7821 bp    DNA     circular  SYN 
DEFINITION  GRIK5 (Myc-DDK-tagged)-Human glutamate receptor, ionotropic, kainate 5 (GRIK5)
ACCESSION   NM_002088
VERSION     NM_002088.4
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3978..4069 
                     /label="Myc-DDK"  
     misc_feature    4099..4684 
                     /label="PolyA signal"  
     misc_feature    4156..4175 
                     /label="Restriction Enzyme"  
     misc_feature    4960..5632 
                     /label="ColE1"  
     misc_feature    6062..6856 
                     /label="Kan/Neo"  
     misc_feature    6891..7193 
                     /label="SV40 ori"  
     misc_feature    7279..7756 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3960..3966 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3960 
                     /label="GRIK5"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCGGCTGAG CTGCTGCTGC TGCTGATTGT TGCCTTCGCC AGCCCCAGCT 
     1081 GCCAGGTGCT CTCATCACTG CGCATGGCTG CAATCCTGGA TGATCAGACA GTGTGTGGCC 
     1141 GCGGTGAGCG TCTGGCCTTG GCCTTGGCCC GGGAGCAGAT CAACGGGATC ATCGAGGTCC 
     1201 CAGCCAAGGC CCGAGTGGAA GTAGACATCT TTGAGCTGCA GCGGGACAGC CAGTACGAGA 
     1261 CCACGGACAC CATGTGTCAG ATCTTACCCA AAGGGGTTGT GTCTGTCCTT GGGCCCTCCT 
     1321 CTAGCCCAGC ATCTGCCTCC ACCGTGAGCC ATATCTGTGG AGAGAAGGAG ATCCCCCACA 
     1381 TCAAGGTGGG TCCCGAGGAG ACACCCCGCC TTCAGTACCT TCGCTTCGCG TCTGTCAGCC 
     1441 TGTACCCCAG TAACGAGGAC GTCAGCTTGG CGGTCTCCCG AATCCTCAAG TCCTTCAACT 
     1501 ACCCCTCGGC CAGCCTCATC TGCGCCAAGG CTGAGTGCCT GCTGCGATTG GAGGAACTGG 
     1561 TGCGTGGCTT CCTCATCTCC AAGGAGACGC TGTCAGTGAG GATGTTGGAC GACAGCCGGG 
     1621 ACCCCACACC ACTGCTCAAG GAGATCCGTG ATGACAAGGT GTCCACCATC ATCATCGACG 
     1681 CCAACGCCTC CATCTCCCAC CTCATCCTCC GTAAGGCCTC GGAACTGGGA ATGACCTCAG 
     1741 CGTTTTACAA GTACATCCTC ACCACCATGG ACTTCCCCAT CCTGCATCTG GACGGTATTG 
     1801 TGGAGGACTC CTCCAACATC CTGGGCTTCT CCATGTTCAA CACGTCCCAC CCCTTCTACC 
     1861 CTGAGTTTGT CCGCAGCCTC AACATGTCCT GGAGGGAGAA CTGTGAAGCC AGCACCTACC 
     1921 TGGGCCCTGC GCTGTCAGCC GCCCTGATGT TTGACGCCGT GCACGTGGTG GTGAGCGCTG 
     1981 TCCGAGAGCT GAACCGCAGC CAGGAGATCG GTGTGAAGCC TCTGGCCTGT ACATCGGCCA 
     2041 ACATTTGGCC CCACGGGACC AGCCTCATGA ACTACCTGCG CATGGTAGAG TATGATGGGC 
     2101 TGACCGGGCG GGTCGAGTTC AACAGCAAAG GGCAGAGAAC CAACTACACC CTGCGCATCC 
     2161 TAGAAAAGTC CCGGCAGGGC CACCGTGAGA TTGGGGTGTG GTACTCTAAC CGCACCCTGG 
     2221 CCATGAATGC CACCACCCTG GACATCAACC TGTCGCAGAC ACTGGCCAAC AAGACCCTGG 
     2281 TGGTCACAAC CATCCTGGAG AACCCATACG TCATGCGCCG GCCCAACTTC CAGGCCCTGT 
     2341 CGGGGAACGA ACGCTTCGAG GGCTTCTGCG TGGACATGCT GCGGGAGCTG GCCGAGCTGC 
     2401 TGCGCTTCCG CTACCGCCTG CGGTTGGTGG AGGATGGGCT GTACGGGGCG CCCGAGCCCA 
     2461 ACGGCTCCTG GACGGGCATG GTTGGCGAGC TCATCAACCG GAAGGCAGAC CTGGCTGTGG 
     2521 CCGCCTTCAC CATCACAGCT GAGCGGGAGA AGGTCATCGA CTTTTCCAAG CCCTTTATGA 
     2581 CCCTGGGGAT CAGCATCCTC TACCGAGTGC ACATGGGCCG CAAGCCTGGC TACTTCTCCT 
     2641 TCCTGGACCC CTTCTCCCCT GCTGTGTGGC TCTTCATGCT TCTTGCCTAC CTGGCTGTCA 
     2701 GCTGCGTCCT GTTTCTGGCT GCCAGGCTGA GCCCCTATGA GTGGTATAAC CCACACCCAT 
     2761 GCCTGCGGGC ACGCCCCCAC ATCCTGGAGA ACCAGTACAC GCTGGGCAAC AGCCTGTGGT 
     2821 TTCCCGTGGG GGGCTTCATG CAGCAGGGCT CGGAGATCAT GCCCCGGGCG CTGTCCACGC 
     2881 GCTGTGTCAG CGGAGTCTGG TGGGCCTTCA CCTTGATCAT CATCTCCTCC TACACGGCCA 
     2941 ACCTGGCCGC CTTCCTCACC GTGCAGCGCA TGGAGGTGCC TGTGGAGTCG GCCGATGACC 
     3001 TGGCAGATCA GACCAACATC GAGTATGGCA CCATCCACGC CGGCTCCACC ATGACCTTCT 
     3061 TCCAGAATTC ACGGTACCAA ACGTACCAGC GCATGTGGAA CTACATGCAG TCGAAGCAGC 
     3121 CCAGCGTGTT CGTCAAGAGC ACAGAAGAGG GCATTGCCCG CGTCCTCAAC TCCCGCTACG 
     3181 CCTTCCTGCT CGAGTCCACC ATGAACGAAT ACCACCGGCG CCTCAACTGC AACCTCACCC 
     3241 AGATCGGGGG ACTCCTCGAC ACCAAGGGCT ACGGCATTGG CATGCCGCTG GGCTCCCCGT 
     3301 TCCGGGATGA GATCACACTG GCCATCCTGC AGCTTCAGGA GAACAACCGG CTGGAGATCC 
     3361 TGAAGCGCAA GTGGTGGGAG GGGGGCCGGT GCCCCAAGGA GGAGGACCAT CGAGCTAAAG 
     3421 GTTTGGGCAT GGAGAACATT GGTGGCATTT TTATCGTGCT CATCTGTGGC CTCATCATTG 
     3481 CTGTCTTCGT GGCGGTCATG GAATTCATAT GGTCCACACG GAGGTCAGCT GAGTCCGAGG 
     3541 AGGTGTCGGT GTGCCAGGAG ATGCTGCAGG AGCTGCGCCA CGCCGTTTCT TGCCGCAAGA 
     3601 CGTCGCGTTC CCGCCGGCGC CGACGCCCGG GCGGCCCGAG CCGGGCCCTG CTGTCACTGC 
     3661 GCGCGGTCCG CGAGATGCGC CTCAGCAACG GCAAGCTCTA CTCGGCCGGC GCGGGCGGGG 
     3721 ATGCGGGCAG CGCGCACGGG GGCCCGCAGC GCCTCCTGGA CGACCCGGGG CCCCCCAGCG 
     3781 GAGCCCGACC CGCCGCCCCC ACCCCCTGCA CCCACGTGCG CGTCTGCCAG GAGTGCCGGC 
     3841 GCATCCAGGC GCTGCGGGCC TCGGGGGCCG GCGCGCCTCC GCGTGGCCTG GGCGTCCCCG 
     3901 CCGAAGCCAC CAGCCCGCCC CGGCCGCGGC CTGGCCCCGC CGGCCCCCGG GAGCTGGCGG 
     3961 AGCACGAGAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     4021 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     4081 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     4141 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     4201 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     4261 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     4321 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     4381 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     4441 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     4501 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     4561 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     4621 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     4681 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     4741 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     4801 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     4861 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     4921 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     4981 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     5041 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     5101 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     5161 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     5221 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     5281 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     5341 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     5401 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     5461 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     5521 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     5581 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     5641 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     5701 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     5761 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     5821 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     5881 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     5941 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     6001 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     6061 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     6121 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     6181 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     6241 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     6301 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     6361 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     6421 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     6481 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     6541 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     6601 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     6661 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     6721 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     6781 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     6841 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     6901 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     6961 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     7021 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     7081 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7141 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7201 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     7261 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     7321 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     7381 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     7441 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     7501 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     7561 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     7621 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     7681 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     7741 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     7801 CAAAAATTTA ACGCGAATTT T
//