LOCUS       RC224671                8475 bp    DNA     circular  SYN 
DEFINITION  RGS3 (Myc-DDK-tagged)-Human regulator of G-protein signaling 3 (RGS3), transcript variant 6
ACCESSION   NM_144488
VERSION     NM_144488.4
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4632..4723 
                     /label="Myc-DDK"  
     misc_feature    4753..5338 
                     /label="PolyA signal"  
     misc_feature    4810..4829 
                     /label="Restriction Enzyme"  
     misc_feature    5614..6286 
                     /label="ColE1"  
     misc_feature    6716..7510 
                     /label="Kan/Neo"  
     misc_feature    7545..7847 
                     /label="SV40 ori"  
     misc_feature    7933..8410 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4614..4620 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4614 
                     /label="RGS3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCTGTAATC CCAGCACTTT GGGAGGTTGA GATGGGCAGA TCGCAAGGTC 
     1081 AGGAGATCGA GACCATCCTG GCTAACAGGT CACATTCAGA CAGCACACCT TTGCCCAATT 
     1141 TTCTTTCTGG ATCTCACCGT CCTGAGTGTT GTACCTGCAG GTTGCTCACA GCCTCTGGAG 
     1201 CCCAAGATAG TCTCCCCTTT GGGAGGAGGC TCTACAGTGG TCCCTGGCGA AGTTGTGAAG 
     1261 AGGTCTGCCA CGTCTCTGTG CTCAGTGTCC TCTCTACATC CTGTGGCTTG AGCCTGAGCT 
     1321 TGCCCATATT CCCTGGCTGG ATGGAGTGGC TAAGCCCTGA TATCGCTCTG CCCAGAAGAG 
     1381 ATGAGTGGAC TCAAACTTCT CCAGCCAGGA AGAGGATCAC GCATGCCAAA GTCCAGGGTG 
     1441 CAGGTCAGCT GAGGCTGTCC ATTGATGCCC AGGACCGGGT TCTGCTGCTT CACATTATAG 
     1501 AAGGTAAAGG CCTGATCAGC AAACAGCCTG GCACCTGTGA TCCGTATGTG AAGATTTCTT 
     1561 TGATCCCTGA AGATAGTAGA CTACGCCACC AGAAGACGCA GACCGTTCCA GACTGCAGAG 
     1621 ACCCGGCTTT CCACGAGCAC TTCTTCTTTC CTGTCCAAGA GGAGGATGAT CAGAAGCGTC 
     1681 TCTTGGTTAC TGTGTGGAAC AGGGCCAGCC AGTCCAGACA GAGTGGACTC ATTGGCTGCA 
     1741 TGAGCTTTGG GGTGAAGTCT CTCCTGACTC CAGACAAGGA GATCAGTGGT TGGTACTACC 
     1801 TCCTAGGGGA GCACCTGGGC CGGACCAAGC ACTTGAAGGT GGCCAGGCGG CGACTGCGGC 
     1861 CGCTGAGAGA CCCGCTGCTG AGAATGCCAG GAGGTGGGGA CACTGAGAAT GGGAAGAAAC 
     1921 TAAAGATCAC CATCCCGAGG GGAAAGGACG GCTTTGGCTT CACCATCTGC TGCGACTCTC 
     1981 CAGTTCGAGT CCAGGCCGTG GATTCCGGGG GTCCGGCGGA ACGGGCAGGG CTGCAGCAGC 
     2041 TGGACACGGT GCTGCAGCTG AATGAGAGGC CTGTGGAGCA CTGGAAATGT GTGGAGCTGG 
     2101 CCCACGAGAT CCGGAGCTGC CCCAGTGAGA TCATCCTACT CGTGTGGCGC ATGGTCCCCC 
     2161 AGGTCAAGCC AGGACCAGAT GGCGGGGTCC TGCGGCGGGC CTCCTGCAAG TCGACACATG 
     2221 ACCTCCAGTC ACCCCCCAAC AAACGGGAGA AGAACTGCAC CCATGGGGTC CAGGCACGGC 
     2281 CTGAGCAGCG CCACAGCTGC CACCTGGTAT GTGACAGCTC TGATGGGCTG CTGCTCGGCG 
     2341 GCTGGGAGCG CTACACCGAG GTGGCCAAGC GCGGGGGCCA GCACACCCTG CCTGCACTGT 
     2401 CCCGTGCCAC TGCCCCCACC GACCCCAACT ACATCATCCT GGCCCCGCTG AATCCTGGGA 
     2461 GCCAGCTGCT CCGGCCTGTG TACCAGGAGG ATACCATCCC CGAAGAATCA GGGAGTCCCA 
     2521 GTAAAGGGAA GTCCTACACA GGCCTGGGGA AGAAGTCCCG GCTGATGAAG ACAGTGCAGA 
     2581 CCATGAAGGG CCACGGGAAC TACCAAAACT GCCCGGTTGT GAGGCCGCAT GCCACGCACT 
     2641 CAAGCTATGG CACCTACGTC ACCCTGGCCC CCAAAGTCCT GGTGTTCCCT GTCTTTGTTC 
     2701 AGCCTCTAGA TCTCTGTAAT CCTGCCCGGA CCCTCCTGCT GTCAGAGGAG CTGCTGCTGT 
     2761 ATGAAGGGAG GAACAAGGCT GCCGAGGTGA CACTGTTTGC CTATTCGGAC CTGCTGCTCT 
     2821 TCACCAAGGA GGACGAGCCT GGCCGCTGCG ACGTCCTGAG GAACCCCCTC TACCTCCAGA 
     2881 GTGTGAAGCT GCAGGAAGGT TCTTCAGAAG ACCTGAAATT CTGCGTGCTC TATCTAGCAG 
     2941 AGAAGGCAGA GTGCTTATTC ACTTTGGAAG CGCACTCGCA GGAGCAGAAG AAGAGAGTGT 
     3001 GCTGGTGCCT GTCGGAGAAC ATCGCCAAGC AGCAACAGCT GGCAGCATCA CCCCCGGACA 
     3061 GCAAGATGTT TGAGACGGAG GCAGATGAGA AGAGGGAGAT GGCCTTGGAG GAAGGGAAGG 
     3121 GGCCTGGTGC CGAGGATTCC CCACCCAGCA AGGAGCCCTC TCCTGGCCAG GAGCTTCCTC 
     3181 CAGGACAAGA CCTTCCACCC AACAAGGACT CCCCTTCTGG GCAGGAACCC GCTCCCAGCC 
     3241 AAGAACCACT GTCCAGCAAA GACTCAGCTA CCTCTGAAGG ATCCCCTCCA GGCCCAGATG 
     3301 CTCCGCCCAG CAAGGATGTG CCACCATGCC AGGAACCCCC TCCAGCCCAA GACCTCTCAC 
     3361 CCTGCCAGGA CCTACCTGCT GGTCAAGAAC CCCTGCCTCA CCAGGACCCT CTACTCACCA 
     3421 AAGACCTCCC TGCCATCCAG GAATCCCCCA CCCGGGACCT TCCACCCTGT CAAGATCTGC 
     3481 CTCCTAGCCA GGTCTCCCTG CCAGCCAAGG CCCTTACTGA GGACACCATG AGCTCCGGGG 
     3541 ACCTACTAGC AGCTACTGGG GACCCACCTG CGGCCCCCAG GCCAGCCTTC GTGATCCCTG 
     3601 AGGTCCGGCT GGATAGCACC TACAGCCAGA AGGCAGGGGC AGAGCAGGGC TGCTCGGGAG 
     3661 ATGAGGAGGA TGCAGAAGAG GCCGAGGAGG TGGAGGAGGG GGAGGAAGGG GAGGAGGACG 
     3721 AGGATGAGGA CACCAGCGAT GACAACTACG GAGAGCGCAG TGAGGCCAAG CGCAGCAGCA 
     3781 TGATCGAGAC GGGCCAGGGG GCTGAGGGTG GCCTCTCACT GCGTGTGCAG AACTCGCTGC 
     3841 GGCGCCGGAC GCACAGCGAG GGCAGCCTGC TGCAGGAGCC CCGAGGGCCC TGCTTTGCCT 
     3901 CCGACACCAC CTTGCACTGC TCAGACGGTG AGGGCGCCGC CTCCACCTGG GGCATGCCTT 
     3961 CGCCCAGCAC CCTCAAGAAA GAGCTGGGCC GCAATGGTGG CTCCATGCAC CACCTTTCCC 
     4021 TCTTCTTCAC AGGACACAGG AAGATGAGCG GGGCTGACAC CGTTGGGGAT GATGACGAAG 
     4081 CCTCCCGGAA GAGAAAGAGC AAAAACCTAG CCAAGGACAT GAAGAACAAG CTGGGGATCT 
     4141 TCAGACGGCG GAATGAGTCC CCTGGAGCCC CTCCCGCGGG CAAGGCAGAC AAAATGATGA 
     4201 AGTCATTCAA GCCCACCTCA GAGGAAGCCC TCAAGTGGGG CGAGTCCTTG GAGAAGCTGC 
     4261 TGGTTCACAA ATACGGGTTA GCAGTGTTCC AAGCCTTCCT TCGCACTGAG TTCAGTGAGG 
     4321 AGAATCTGGA GTTCTGGTTG GCTTGTGAGG ACTTCAAGAA GGTCAAGTCA CAGTCCAAGA 
     4381 TGGCATCCAA GGCCAAGAAG ATCTTTGCTG AATACATCGC GATCCAGGCA TGCAAGGAGG 
     4441 TCAACCTGGA CTCCTACACG CGGGAGCACA CCAAGGACAA CCTGCAGAGC GTCACGCGGG 
     4501 GCTGCTTCGA CCTGGCACAG AAGCGCATCT TCGGGCTCAT GGAAAAGGAC TCGTACCCTC 
     4561 GCTTTCTCCG TTCTGACCTC TACCTGGACC TTATTAACCA GAAGAAGATG AGTCCCCCGC 
     4621 TTACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     4681 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     4741 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     4801 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     4861 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     4921 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     4981 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     5041 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     5101 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     5161 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     5221 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     5281 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     5341 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     5401 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     5461 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     5521 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     5581 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     5641 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     5701 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     5761 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     5821 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     5881 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     5941 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     6001 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     6061 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     6121 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     6181 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     6241 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     6301 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     6361 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     6421 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     6481 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     6541 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     6601 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     6661 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     6721 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     6781 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     6841 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     6901 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     6961 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     7021 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     7081 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     7141 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     7201 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     7261 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     7321 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     7381 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     7441 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     7501 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     7561 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     7621 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     7681 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     7741 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     7801 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7861 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     7921 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     7981 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     8041 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     8101 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     8161 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     8221 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     8281 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     8341 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     8401 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     8461 TTTAACGCGA ATTTT
//