LOCUS       RC224649                8469 bp    DNA     circular  SYN 
DEFINITION  AHI1 (Myc-DDK-tagged)-Human Abelson helper integration site 1 (AHI1), transcript variant 2
ACCESSION   NM_017651
VERSION     NM_017651.4
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4626..4717 
                     /label="Myc-DDK"  
     misc_feature    4747..5332 
                     /label="PolyA signal"  
     misc_feature    4804..4823 
                     /label="Restriction Enzyme"  
     misc_feature    5608..6280 
                     /label="ColE1"  
     misc_feature    6710..7504 
                     /label="Kan/Neo"  
     misc_feature    7539..7841 
                     /label="SV40 ori"  
     misc_feature    7927..8404 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4608..4614 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4608 
                     /label="AHI1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCTACAGCT GAGAGTGAAG CAAAAGTAAA AACCAAAGTT CGCTTTGAAG 
     1081 AATTGCTTAA GACCCACAGT GATCTAATGC GTGAAAAGAA AAAACTGAAG AAAAAACTTG 
     1141 TCAGGTCTGA AGAAAACATC TCACCTGACA CTATTAGAAG CAATCTTCAC TATATGAAAG 
     1201 AAACTACAAG TGATGATCCC GACACTATTA GAAGCAATCT TCCCCATATT AAAGAAACTA 
     1261 CAAGTGATGA TGTAAGTGCT GCTAACACTA ACAACCTGAA GAAGAGCACG AGAGTCACTA 
     1321 AAAACAAATT GAGGAACACA CAGTTAGCAA CTGAAAATCC TAATGGTGAT GCTAGTGTAG 
     1381 AGGAAGACAA ACAAGGAAAG CCAAATAAAA AGGTGATAAA GACGGTGCCC CAGTTGACTA 
     1441 CACAAGACCT GAAACCGGAA ACTCCTGAGA ATAAGGTTGA TTCTACACAC CAGAAAACAC 
     1501 ATACAAAGCC ACAGCCAGGC GTTGATCATC AGAAAAGTGA GAAGGCAAAT GAGGGAAGAG 
     1561 AAGAGACTGA TTTAGAAGAG GATGAAGAAT TGATGCAAGC ATATCAGTGC CATGTAACTG 
     1621 AAGAAATGGC AAAGGAGATT AAGAGGAAAA TAAGAAAGAA ACTGAAAGAA CAGTTGACTT 
     1681 ACTTTCCCTC AGATACTTTA TTCCATGATG ACAAACTAAG CAGTGAAAAA AGGAAAAAGA 
     1741 AAAAGGAAGT TCCAGTCTTC TCTAAAGCTG AAACAAGTAC ATTGACCATC TCTGGTGACA 
     1801 CAGTTGAAGG TGAACAAAAG AAAGAATCTT CAGTTAGATC AGTTTCTTCA GATTCTCATC 
     1861 AAGATGATGA AATAAGCTCA ATGGAACAAA GCACAGAAGA CAGCATGCAA GATGATACAA 
     1921 AACCTAAACC AAAAAAAACA AAAAAGAAGA CTAAAGCAGT TGCAGATAAT AATGAAGATG 
     1981 TTGATGGTGA TGGTGTTCAT GAAATAACAA GCCGAGATAG CCCGGTTTAT CCCAAATGTT 
     2041 TGCTTGATGA TGACCTTGTC TTGGGAGTTT ACATTCACCG AACTGATAGA CTTAAGTCAG 
     2101 ATTTTATGAT TTCTCACCCA ATGGTAAAAA TTCATGTGGT TGATGAGCAT ACTGGTCAAT 
     2161 ATGTCAAGAA AGATGATAGT GGACGGCCTG TTTCATCTTA CTATGAAAAA GAGAATGTGG 
     2221 ATTATATTCT TCCTATTATG ACCCAGCCAT ATGATTTTAA ACAGTTAAAA TCAAGACTTC 
     2281 CAGAGTGGGA AGAACAAATT GTATTTAATG AAAATTTTCC CTATTTGCTT CGAGGCTCTG 
     2341 ATGAGAGTCC TAAAGTCATC CTGTTCTTTG AGATTCTTGA TTTCTTAAGC GTGGATGAAA 
     2401 TTAAGAATAA TTCTGAGGTT CAAAACCAAG AATGTGGCTT TCGGAAAATT GCCTGGGCAT 
     2461 TTCTTAAGCT TCTGGGAGCC AATGGAAATG CAAACATCAA CTCAAAACTT CGCTTGCAGC 
     2521 TATATTACCC ACCTACTAAG CCTCGATCCC CATTAAGTGT TGTTGAGGCA TTTGAATGGT 
     2581 GGTCAAAATG TCCAAGAAAT CATTACCCAT CAACACTGTA CGTAACTGTA AGAGGACTGA 
     2641 AAGTTCCAGA CTGTATAAAG CCATCTTACC GCTCTATGAT GGCTCTTCAG GAGGAAAAAG 
     2701 GTAAACCAGT GCATTGTGAA CGTCACCATG AGTCAAGCTC AGTAGACACA GAACCTGGAT 
     2761 TAGAAGAGTC AAAGGAAGTA ATAAAGTGGA AACGACTCCC TGGGCAGGCT TGCCGTATCC 
     2821 CAAACAAACA CCTCTTCTCA CTAAATGCAG GAGAACGAGG ATGTTTTTGT CTTGATTTCT 
     2881 CCCACAATGG AAGAATATTA GCAGCAGCTT GTGCCAGCCG GGATGGATAT CCAATTATTT 
     2941 TATATGAAAT TCCTTCTGGA CGTTTCATGA GAGAATTGTG TGGCCACCTC AATATCATTT 
     3001 ATGATCTTTC CTGGTCAAAA GATGATCACT ACATCCTTAC TTCATCATCT GATGGCACTG 
     3061 CCAGGATATG GAAAAATGAA ATAAACAATA CAAATACTTT CAGAGTTTTA CCTCATCCTT 
     3121 CTTTTGTTTA CACGGCTAAA TTCCATCCAG CTGTAAGAGA GCTAGTAGTT ACAGGATGCT 
     3181 ATGATTCCAT GATACGGATA TGGAAAGTTG AGATGAGAGA AGATTCTGCC ATATTGGTCC 
     3241 GACAGTTTGA TGTTCACAAA AGTTTTATCA ACTCACTTTG TTTTGATACT GAAGGTCATC 
     3301 ATATGTATTC AGGAGATTGT ACAGGGGTGA TTGTTGTTTG GAATACCTAT GTCAAGATTA 
     3361 ATGATTTGGA ACATTCAGTG CACCACTGGA CTATAAATAA GGAAATTAAA GAAACTGAGT 
     3421 TTAAGGGAAT TCCAATAAGT TATTTGGAGA TTCATCCCAA TGGAAAACGT TTGTTAATCC 
     3481 ATACCAAAGA CAGTACTTTG AGAATTATGG ATCTCCGGAT ATTAGTAGCA AGGAAGTTTG 
     3541 TAGGAGCAGC AAATTATCGG GAGAAGATTC ATAGTACTTT GACTCCATGT GGGACTTTTC 
     3601 TGTTTGCTGG AAGTGAGGAT GGTATAGTGT ATGTTTGGAA CCCAGAAACA GGAGAACAAG 
     3661 TAGCCATGTA TTCTGACTTG CCATTCAAGT CACCCATTCG AGACATTTCT TATCATCCAT 
     3721 TTGAAAATAT GGTTGCATTC TGTGCATTTG GGCAAAATGA GCCAATTCTT CTGTATATTT 
     3781 ACGATTTCCA TGTTGCCCAG CAGGAGGCTG AAATGTTCAA ACGCTACAAT GGAACATTTC 
     3841 CATTACCTGG AATACACCAA AGTCAAGATG CCCTATGTAC CTGTCCAAAA CTACCCCATC 
     3901 AAGGCTCTTT TCAGATTGAT GAATTTGTCC ACACTGAAAG TTCTTCAACG AAGATGCAGC 
     3961 TAGTAAAACA GAGGCTTGAA ACTGTCACAG AGGTGATACG TTCCTGTGCT GCAAAAGTCA 
     4021 ACAAAAATCT CTCATTTACT TCACCACCAG CAGTTTCCTC ACAACAGTCT AAGTTAAAGC 
     4081 AGTCAAACAT GCTGACCGCT CAAGAGATTC TACATCAGTT TGGTTTCACT CAGACCGGGA 
     4141 TTATCAGCAT AGAAAGAAAG CCTTGTAACC ATCAGGTAGA TACAGCACCA ACGGTAGTGG 
     4201 CTCTTTATGA CTACACAGCG AATCGATCAG ATGAACTAAC CATCCATCGC GGAGACATTA 
     4261 TCCGAGTGTT TTTCAAAGAT AATGAAGACT GGTGGTATGG CAGCATAGGA AAGGGACAGG 
     4321 AAGGTTATTT TCCAGCTAAT CATGTGGCTA GTGAAACACT GTATCAAGAA CTGCCTCCTG 
     4381 AGATAAAGGA GCGATCCCCT CCTTTAAGCC CTGAGGAAAA AACTAAAATA GAAAAATCTC 
     4441 CAGCTCCTCA AAAGCAATCA ATCAATAAGA ACAAGTCCCA GGACTTCAGA CTAGGCTCAG 
     4501 AATCTATGAC ACATTCTGAA ATGAGAAAAG AACAGAGCCA TGAGGACCAA GGACACATAA 
     4561 TGGATACACG GATGAGGAAG AACAAGCAAG CAGGCAGAAA AGTCACTCTA ATAGAGACGC 
     4621 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     4681 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     4741 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     4801 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     4861 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     4921 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     4981 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     5041 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     5101 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     5161 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     5221 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     5281 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     5341 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     5401 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     5461 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     5521 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     5581 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     5641 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     5701 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     5761 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     5821 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     5881 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     5941 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     6001 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     6061 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     6121 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     6181 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     6241 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     6301 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     6361 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     6421 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     6481 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     6541 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     6601 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     6661 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     6721 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     6781 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     6841 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     6901 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     6961 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     7021 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     7081 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     7141 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     7201 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     7261 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     7321 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     7381 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     7441 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     7501 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     7561 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     7621 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     7681 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     7741 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     7801 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7861 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     7921 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     7981 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     8041 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     8101 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     8161 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     8221 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     8281 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     8341 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     8401 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     8461 GCGAATTTT
//