LOCUS       RC224642                8490 bp    DNA     circular  SYN 
DEFINITION  PTCH2 (Myc-DDK-tagged)-Human patched 2 (PTCH2), transcript variant 1
ACCESSION   NM_003738
VERSION     NM_003738.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4647..4738 
                     /label="Myc-DDK"  
     misc_feature    4768..5353 
                     /label="PolyA signal"  
     misc_feature    4825..4844 
                     /label="Restriction Enzyme"  
     misc_feature    5629..6301 
                     /label="ColE1"  
     misc_feature    6731..7525 
                     /label="Kan/Neo"  
     misc_feature    7560..7862 
                     /label="SV40 ori"  
     misc_feature    7948..8425 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4629..4635 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4629 
                     /label="PTCH2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACTCGATCG CCGCCCCTCA GAGAGCTGCC CCCGAGTTAC ACACCCCCAG 
     1081 CTCGAACCGC AGCACCCCAG ATCCTAGCTG GGAGCCTGAA GGCTCCACTC TGGCTTCGTG 
     1141 CTTACTTCCA GGGCCTGCTC TTCTCTCTGG GATGCGGGAT CCAGAGACAT TGTGGCAAAG 
     1201 TGCTCTTTCT GGGACTGTTG GCCTTTGGGG CCCTGGCATT AGGTCTCCGC ATGGCCATTA 
     1261 TTGAGACAAA CTTGGAACAG CTCTGGGTAG AAGTGGGCAG CCGGGTGAGC CAGGAGCTGC 
     1321 ATTACACCAA GGAGAAGCTG GGGGAGGAGG CTGCATACAC CTCTCAGATG CTGATACAGA 
     1381 CCGCACGCCA GGAGGGAGAG AACATCCTCA CACCCGAAGC ACTTGGCCTC CACCTCCAGG 
     1441 CAGCCCTCAC TGCCAGTAAA GTCCAAGTAT CACTCTATGG GAAGTCCTGG GATTTGAACA 
     1501 AAATCTGCTA CAAGTCAGGA GTTCCCCTTA TTGAAAATGG AATGATTGAG CGGATGATTG 
     1561 AGAAGCTGTT TCCGTGCGTG ATCCTCACCC CCCTCGACTG CTTCTGGGAG GGAGCCAAAC 
     1621 TCCAAGGGGG CTCCGCCTAC CTGCCCGGCC GCCCGGATAT CCAGTGGACC AACCTGGATC 
     1681 CAGAGCAGCT GCTGGAGGAG CTGGGTCCCT TTGCCTCCCT TGAGGGCTTC CGGGAGCTGC 
     1741 TAGACAAGGC ACAGGTGGGC CAGGCCTACG TGGGGCGGCC CTGTCTGCAC CCTGATGACC 
     1801 TCCACTGCCC ACCTAGTGCC CCCAACCATC ACAGCAGGCA GGCTCCCAAT GTGGCTCACG 
     1861 AGCTGAGTGG GGGCTGCCAT GGCTTCTCCC ACAAATTCAT GCACTGGCAG GAGGAATTGC 
     1921 TGCTGGGAGG CATGGCCAGA GACCCCCAAG GAGAGCTGCT GAGGGCAGAG GCCCTGCAGA 
     1981 GCACCTTCTT GCTGATGAGT CCCCGCCAGC TGTACGAGCA TTTCCGGGGT GACTATCAGA 
     2041 CACATGACAT TGGCTGGAGT GAGGAGCAGG CCAGCACAGT GCTACAAGCC TGGCAGCGGC 
     2101 GCTTTGTGCA GCTGGCCCAG GAGGCCCTGC CTGAGAACGC TTCCCAGCAG ATCCATGCCT 
     2161 TCTCCTCCAC CACCCTGGAT GACATCCTGC ATGCGTTCTC TGAAGTCAGT GCTGCCCGTG 
     2221 TGGTGGGAGG CTATCTGCTC ATGCTGGCCT ATGCCTGTGT GACCATGCTG CGGTGGGACT 
     2281 GCGCCCAGTC CCAGGGTTCC GTGGGCCTTG CCGGGGTACT GCTGGTGGCC CTGGCGGTGG 
     2341 CCTCAGGCCT TGGGCTCTGT GCCCTGCTCG GCATCACCTT CAATGCTGCC ACTACCCAGG 
     2401 TGCTGCCCTT CTTGGCTCTG GGAATCGGCG TGGATGACGT ATTCCTGCTG GCGCATGCCT 
     2461 TCACAGAGGC TCTGCCTGGC ACCCCTCTCC AGGAGCGCAT GGGCGAGTGT CTGCAGCGCA 
     2521 CGGGCACCAG TGTCGTACTC ACATCCATCA ACAACATGGC CGCCTTCCTC ATGGCTGCCC 
     2581 TCGTTCCCAT CCCTGCGCTG CGAGCCTTCT CCCTACAGGC GGCCATAGTG GTTGGCTGCA 
     2641 CCTTTGTAGC CGTGATGCTT GTCTTCCCAG CCATCCTCAG CCTGGACCTA CGGCGGCGCC 
     2701 ACTGCCAGCG CCTTGATGTG CTCTGCTGCT TCTCCAGTCC CTGCTCTGCT CAGGTGATTC 
     2761 AGATCCTGCC CCAGGAGCTG GGGGACGGGA CAGTACCAGT GGGCATTGCC CACCTCACTG 
     2821 CCACAGTTCA AGCCTTTACC CACTGTGAAG CCAGCAGCCA GCATGTGGTC ACCATCCTGC 
     2881 CTCCCCAAGC CCACCTGGTG CCCCCACCTT CTGACCCACT GGGCTCTGAG CTCTTCAGCC 
     2941 CTGGAGGGTC CACACGGGAC CTTCTAGGCC AGGAGGAGGA GACAAGGCAG AAGGCAGCCT 
     3001 GCAAGTCCCT GCCCTGTGCC CGCTGGAATC TTGCCCATTT CGCCCGCTAT CAGTTTGCCC 
     3061 CGTTGCTGCT CCAGTCACAT GCTAAGGCCA TCGTGCTGGT GCTCTTTGGT GCTCTTCTGG 
     3121 GCCTGAGCCT CTACGGAGCC ACCTTGGTGC AAGACGGCCT GGCCCTGACG GATGTGGTGC 
     3181 CTCGGGGCAC CAAGGAGCAT GCCTTCCTGA GCGCCCAGCT CAGGTACTTC TCCCTGTACG 
     3241 AGGTGGCCCT GGTGACCCAG GGTGGCTTTG ACTACGCCCA CTCCCAACGC GCCCTCTTTG 
     3301 ATCTGCACCA GCGCTTCAGT TCCCTCAAGG CGGTGCTGCC CCCACCGGCC ACCCAGGCAC 
     3361 CCCGCACCTG GCTGCACTAT TACCGCAACT GGCTACAGGG AATCCAGGCT GCCTTTGACC 
     3421 AGGACTGGGC TTCTGGGCGC ATCACCCGCC ACTCGTACCG CAATGGCTCT GAGGATGGGG 
     3481 CCCTGGCCTA CAAGCTGCTC ATCCAGACTG GAGACGCCCA GGAGCCTCTG GATTTCAGCC 
     3541 AGCTGACCAC AAGGAAGCTG GTGGACAGAG AGGGACTGAT TCCACCCGAG CTCTTCTACA 
     3601 TGGGGCTGAC CGTGTGGGTG AGCAGTGACC CCCTGGGTCT GGCAGCCTCA CAGGCCAACT 
     3661 TCTACCCCCC ACCTCCTGAA TGGCTGCACG ACAAATACGA CACCACGGGG GAGAACCTTC 
     3721 GCATCCCGCC AGCTCAGCCC TTGGAGTTTG CCCAGTTCCC CTTCCTGCTG CGTGGCCTCC 
     3781 AGAAGACTGC AGACTTTGTG GAGGCCATCG AGGGGGCCCG GGCAGCATGC GCAGAGGCCG 
     3841 GCCAGGCTGG GGTGCACGCC TACCCCAGCG GCTCCCCCTT CCTCTTCTGG GAACAGTATC 
     3901 TGGGCCTGCG GCGCTGCTTC CTGCTGGCCG TCTGCATCCT GCTGGTGTGC ACTTTCCTCG 
     3961 TCTGTGCTCT GCTGCTCCTC AACCCCTGGA CGGCTGGCCT CATAGTGCTG GTCCTGGCGA 
     4021 TGATGACAGT GGAACTCTTT GGTATCATGG GTTTCCTGGG CATCAAGCTG AGTGCCATCC 
     4081 CCGTGGTGAT CCTTGTGGCC TCTGTAGGCA TTGGCGTTGA GTTCACAGTC CACGTGGCTC 
     4141 TGGGCTTCCT GACCACCCAG GGCAGCCGGA ACCTGCGGGC CGCCCATGCC CTTGAGCACA 
     4201 CATTTGCCCC CGTGACCGAT GGGGCCATCT CCACATTGCT GGGTCTGCTC ATGCTTGCTG 
     4261 GTTCCCACTT TGACTTCATT GTAAGGTACT TCTTTGCGGC GCTGACAGTG CTCACGCTCC 
     4321 TGGGCCTCCT CCATGGACTC GTGCTGCTGC CTGTGCTGCT GTCCATCCTG GGCCCGCCGC 
     4381 CAGAGGTGAT ACAGATGTAC AAGGAAAGCC CAGAGATCCT GAGTCCACCA GCTCCACAGG 
     4441 GAGGCGGGCT TAGGTGGGGG GCATCCTCCT CCCTGCCCCA GAGCTTTGCC AGAGTGACTA 
     4501 CCTCCATGAC CGTGGCCATC CACCCACCCC CCCTGCCTGG TGCCTACATC CATCCAGCCC 
     4561 CTGATGAGCC CCCTTGGTCC CCTGCTGCCA CTAGCTCTGG CAACCTCAGT TCCAGGGGAC 
     4621 CAGGTCCAGC CACTGGGACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     4681 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     4741 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     4801 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     4861 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     4921 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     4981 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     5041 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     5101 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     5161 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     5221 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     5281 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     5341 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     5401 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     5461 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     5521 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     5581 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     5641 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     5701 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     5761 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     5821 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     5881 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     5941 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     6001 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     6061 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     6121 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     6181 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     6241 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     6301 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     6361 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     6421 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     6481 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     6541 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     6601 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     6661 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     6721 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     6781 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     6841 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     6901 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     6961 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     7021 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     7081 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     7141 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     7201 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     7261 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     7321 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     7381 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     7441 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     7501 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     7561 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     7621 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     7681 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     7741 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7801 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7861 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     7921 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     7981 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     8041 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     8101 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     8161 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     8221 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     8281 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     8341 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     8401 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     8461 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//