LOCUS       RC224578                8178 bp    DNA     circular  SYN 
DEFINITION  SLC12A6 (Myc-DDK-tagged)-Human solute carrier family 12 (potassium/chloride transporters), member 6 (SLC12A6), transcript variant 2
ACCESSION   NM_005135
VERSION     NM_005135.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4335..4426 
                     /label="Myc-DDK"  
     misc_feature    4456..5041 
                     /label="PolyA signal"  
     misc_feature    4513..4532 
                     /label="Restriction Enzyme"  
     misc_feature    5317..5989 
                     /label="ColE1"  
     misc_feature    6419..7213 
                     /label="Kan/Neo"  
     misc_feature    7248..7550 
                     /label="SV40 ori"  
     misc_feature    7636..8113 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4317..4323 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4317 
                     /label="SLC12A6"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCACATTTT ACTGTGACTA AGGTAGAGGA CCCAGAGGAG GGGGCAGCGG 
     1081 CTTCGATCTC TCAAGAGCCT AGTTTAGCAG ACATAAAAGC CCGGATTCAG GATTCAGATG 
     1141 AACCAGACCT GAGTCAGAAC TCCATCACAG GGGAACACAG CCAACTGTTA GACGACGGAC 
     1201 ATAAGAAAGC TCGAAATGCT TATCTCAATA ATTCCAATTA TGAAGAAGGA GATGAATATT 
     1261 TTGATAAAAA TTTGGCACTC TTTGAGGAAG AAATGGACAC CAGACCGAAG GTGTCTTCCC 
     1321 TCCTCAACCG CATGGCCAAT TACACTAATC TGACTCAAGG AGCAAAGGAA CATGAAGAGG 
     1381 CAGAAAACAT CACTGAAGGG AAAAAGAAGC CCACCAAGAC CCCCCAAATG GGTACCTTCA 
     1441 TGGGTGTCTA CCTCCCATGT CTACAAAATA TTTTTGGAGT GATCCTTTTT TTACGCCTTA 
     1501 CATGGGTGGT GGGCACAGCT GGAGTTCTTC AGGCTTTTGC AATTGTCCTT ATCTGCTGCT 
     1561 GCTGTACAAT GTTGACTGCT ATCTCCATGA GTGCCATTGC CACTAATGGA GTGGTGCCAG 
     1621 CTGGGGGCTC ATACTTTATG ATTTCCCGGG CACTGGGCCC AGAGTTTGGT GGGGCTGTTG 
     1681 GCCTCTGCTT TTATCTTGGT ACCACATTTG CAGCAGCCAT GTACATCCTT GGTGCCATTG 
     1741 AAATCTTTCT GGTCTATATC GTCCCCCGAG CTGCCATCTT TCACAGTGAT GACGCACTCA 
     1801 AGGAATCAGC AGCCATGCTA AATAACATGC GTGTCTACGG CACAGCTTTC TTGGTCCTTA 
     1861 TGGTATTAGT GGTATTTATC GGCGTACGCT ATGTGAACAA GTTTGCCTCA CTTTTCCTGG 
     1921 CCTGTGTCAT TGTGTCCATC TTGGCCATCT ATGCTGGAGC CATCAAGTCT TCTTTTGCTC 
     1981 CTCCACACTT CCCGGTCTGC ATGCTGGGTA ACCGCACCCT TTCATCAAGA CACATTGACG 
     2041 TTTGCTCTAA GACCAAGGAA ATTAACAACA TGACAGTCCC ATCAAAGTTA TGGGGATTCT 
     2101 TCTGTAACTC GAGTCAATTT TTCAATGCCA CCTGTGATGA ATACTTTGTT CACAATAACG 
     2161 TCACTTCAAT CCAGGGCATT CCTGGATTGG CTAGTGGTAT AATTACAGAG AATCTTTGGA 
     2221 GTAATTACCT ACCCAAGGGA GAGATCATCG AAAAGCCTTC AGCCAAATCT TCTGATGTCT 
     2281 TAGGCAGCTT AAACCATGAA TATGTTCTTG TTGACATCAC CACCTCCTTC ACGCTTCTGG 
     2341 TGGGAATCTT CTTTCCCTCT GTTACAGGTA TCATGGCTGG ATCAAACAGA TCTGGAGATC 
     2401 TGAAAGATGC TCAGAAGTCT ATTCCGATTG GTACTATCCT TGCCATCCTG ACCACCTCCT 
     2461 TTGTTTATTT AAGCAATGTT GTCCTTTTTG GTGCATGTAT TGAAGGGGTT GTTCTCAGAG 
     2521 ACAAGTTCGG TGATGCTGTG AAAGGTAATT TGGTGGTAGG CACCTTATCT TGGCCATCCC 
     2581 CATGGGTGAT TGTTATTGGC TCCTTCTTTT CAACATGTGG GGCTGGACTT CAGAGCCTCA 
     2641 CAGGTGCACC GAGGCTGCTA CAAGCTATTG CCAAGGATAA CATCATACCG TTTCTGAGGG 
     2701 TTTTTGGCCA CAGCAAAGCC AATGGGGAAC CTACCTGGGC TTTACTTCTA ACTGCTGCCA 
     2761 TTGCAGAGCT TGGAATACTC ATTGCCTCCC TGGATCTTGT GGCCCCAATT CTTTCCATGT 
     2821 TTTTTCTCAT GTGTTACCTC TTTGTAAACT TGGCATGTGC CTTGCAAACA TTACTTCGAA 
     2881 CACCCAACTG GAGACCCCGA TTCCGCTACT ACCATTGGGC CCTTTCTTTC ATGGGAATGA 
     2941 GTATCTGTCT GGCTCTGATG TTCATTTCTT CCTGGTATTA TGCCATTGTA GCCATGGTAA 
     3001 TAGCTGGTAT GATCTACAAG TACATTGAAT ACCAAGGAGC TGAGAAAGAA TGGGGTGATG 
     3061 GTATCCGTGG GCTGTCCCTC AGTGCAGCCC GGTTTGCTTT GCTTCGATTG GAGGAAGGAC 
     3121 CTCCACACAC TAAAAACTGG AGGCCTCAGT TGCTTGTATT ACTGAAACTA GATGAAGACT 
     3181 TACATGTCAA GCATCCTCGC CTCCTCACCT TTGCCTCACA GCTCAAAGCA GGAAAAGGTC 
     3241 TCACTATTGT GGGCTCTGTC ATCGTGGGGA ACTTCCTAGA GAACTACGGT GAAGCTTTAG 
     3301 CTGCTGAGCA GACCATAAAG CACCTAATGG AGGCAGAGAA GGTAAAAGGA TTCTGCCAGC 
     3361 TGGTGGTGGC CGCCAAGCTG AGAGAGGGCA TTTCCCACCT CATCCAGTCA TGTGGCCTTG 
     3421 GGGGCATGAA GCACAACACG GTGGTGATGG GCTGGCCTAA TGGCTGGCGT CAAAGCGAAG 
     3481 ATGCCCGCGC TTGGAAGACT TTTATTGGCA CAGTTCGAGT GACAACTGCT GCCCATCTTG 
     3541 CACTGCTGGT GGCTAAAAAC ATCTCCTTCT TTCCCAGCAA TGTGGAGCAA TTTTCTGAGG 
     3601 GCAACATTGA TGTGTGGTGG ATTGTGCATG ATGGGGGGAT GCTTATGCTA CTACCATTCC 
     3661 TACTGAAACA GCACAAGGTG TGGCGAAAGT GCAGCATACG GATCTTCACA GTAGCCCAAT 
     3721 TAGAAGACAA CAGTATCCAA ATGAAGAAGG ACCTAGCCAC CTTCCTATAT CACTTACGCA 
     3781 TTGAGGCGGA GGTAGAAGTG GTGGAGATGC ATGACAGTGA TATATCAGCA TATACTTACG 
     3841 AGCGCACTTT GATGATGGAA CAAAGGTCCC AGATGCTCCG GCACATGCGG CTATCCAAAA 
     3901 CAGAGCGAGA CAGAGAGGCA CAATTGGTGA AAGACCGAAA CTCAATGCTA CGATTGACCA 
     3961 GCATTGGCTC TGATGAGGAC GAAGAGACAG AAACCTATCA GGAGAAGGTG CACATGACTT 
     4021 GGACAAAAGA CAAGTACATG GCATCCCGGG GACAAAAAGC GAAGTCAATG GAAGGATTCC 
     4081 AGGACCTGCT TAACATGCGT CCGGACCAGT CCAATGTGAG GCGGATGCAT ACAGCAGTGA 
     4141 AACTCAACGA GGTTATAGTT AACAAGTCCC ATGAAGCAAA GCTGGTTTTA TTGAATATGC 
     4201 CAGGGCCACC CCGAAACCCT GAGGGTGATG AAAACTACAT GGAGTTCCTA GAGGTGCTTA 
     4261 CCGAGGGACT AGAGCGAGTC CTACTTGTCC GGGGTGGTGG CAGTGAAGTG ATCACCATTT 
     4321 ATTCAACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     4381 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     4441 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     4501 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     4561 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     4621 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     4681 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     4741 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     4801 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     4861 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     4921 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     4981 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     5041 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     5101 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     5161 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     5221 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     5281 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     5341 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     5401 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     5461 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     5521 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     5581 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     5641 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     5701 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     5761 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     5821 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     5881 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     5941 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     6001 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     6061 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     6121 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     6181 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     6241 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     6301 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     6361 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     6421 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     6481 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     6541 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     6601 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     6661 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     6721 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     6781 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     6841 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     6901 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     6961 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     7021 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     7081 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     7141 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     7201 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     7261 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     7321 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     7381 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     7441 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7501 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7561 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     7621 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     7681 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     7741 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     7801 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     7861 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     7921 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     7981 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     8041 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     8101 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     8161 AAATTTAACG CGAATTTT
//