LOCUS       RC224561                7614 bp    DNA     circular  SYN 
DEFINITION  LIG4 (Myc-DDK-tagged)-Human ligase IV, DNA, ATP-dependent (LIG4), transcript variant 3
ACCESSION   NM_001098268
VERSION     NM_001098268.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3771..3862 
                     /label="Myc-DDK"  
     misc_feature    3892..4477 
                     /label="PolyA signal"  
     misc_feature    3949..3968 
                     /label="Restriction Enzyme"  
     misc_feature    4753..5425 
                     /label="ColE1"  
     misc_feature    5855..6649 
                     /label="Kan/Neo"  
     misc_feature    6684..6986 
                     /label="SV40 ori"  
     misc_feature    7072..7549 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3753..3759 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3753 
                     /label="LIG4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTGCCTCA CAAACTTCAC AAACTGTTGC ATCTCACGTT CCTTTTGCAG 
     1081 ATTTGTGTTC AACTTTAGAA CGAATACAGA AAAGTAAAGG ACGTGCAGAA AAAATCAGAC 
     1141 ACTTCAGGGA ATTTTTAGAT TCTTGGAGAA AATTTCATGA TGCTCTTCAT AAGAACCACA 
     1201 AAGATGTCAC AGACTCTTTT TATCCAGCAA TGAGACTAAT TCTTCCTCAG CTAGAAAGAG 
     1261 AGAGAATGGC CTATGGAATT AAAGAAACTA TGCTTGCTAA GCTTTATATT GAGTTGCTTA 
     1321 ATTTACCTAG AGATGGAAAA GATGCCCTCA AACTTTTAAA CTACAGAACA CCCACTGGAA 
     1381 CTCATGGAGA TGCTGGAGAC TTTGCAATGA TTGCATATTT TGTGTTGAAG CCAAGATGTT 
     1441 TACAGAAAGG AAGTTTAACC ATACAGCAAG TAAACGACCT TTTAGACTCA ATTGCCAGCA 
     1501 ATAATTCTGC TAAAAGAAAA GACCTAATAA AAAAGAGCCT TCTTCAACTT ATAACTCAGA 
     1561 GTTCAGCACT TGAGCAAAAG TGGCTTATAC GGATGATCAT AAAGGATTTA AAGCTTGGTG 
     1621 TTAGTCAGCA AACTATCTTT TCTGTTTTTC ATAATGATGC TGCTGAGTTG CATAATGTCA 
     1681 CTACAGATCT GGAAAAAGTC TGTAGGCAAC TGCATGATCC TTCTGTAGGA CTCAGTGATA 
     1741 TTTCTATCAC TTTATTTTCT GCATTTAAAC CAATGCTAGC TGCTATTGCA GATATTGAGC 
     1801 ACATTGAGAA GGATATGAAA CATCAGAGTT TCTACATAGA AACCAAGCTA GATGGTGAAC 
     1861 GTATGCAAAT GCACAAAGAT GGAGATGTAT ATAAATACTT CTCTCGAAAT GGATATAACT 
     1921 ACACTGATCA GTTTGGTGCT TCTCCTACTG AAGGTTCTCT TACCCCATTC ATTCATAATG 
     1981 CATTCAAAGC AGATATACAA ATCTGTATTC TTGATGGTGA GATGATGGCC TATAATCCTA 
     2041 ATACACAAAC TTTCATGCAA AAGGGAACTA AGTTTGATAT TAAAAGAATG GTAGAGGATT 
     2101 CTGATCTGCA AACTTGTTAT TGTGTTTTTG ATGTATTGAT GGTTAATAAT AAAAAGCTAG 
     2161 GGCATGAGAC TCTGAGAAAG AGGTATGAGA TTCTTAGTAG TATTTTTACA CCAATTCCAG 
     2221 GTAGAATAGA AATAGTGCAG AAAACACAAG CTCATACTAA GAATGAAGTA ATTGATGCAT 
     2281 TGAATGAAGC AATAGATAAA AGAGAAGAGG GAATTATGGT AAAACAACCT CTATCCATCT 
     2341 ACAAGCCAGA CAAAAGAGGT GAAGGGTGGT TAAAAATTAA ACCAGAGTAT GTCAGTGGAC 
     2401 TAATGGATGA ATTGGACATT TTAATTGTTG GAGGATATTG GGGTAAAGGA TCACGGGGTG 
     2461 GAATGATGTC TCATTTTCTG TGTGCAGTAG CAGAGAAGCC CCCTCCTGGT GAGAAGCCAT 
     2521 CTGTGTTTCA TACTCTCTCT CGTGTTGGGT CTGGCTGCAC CATGAAAGAA CTGTATGATC 
     2581 TGGGTTTGAA ATTGGCCAAG TATTGGAAGC CTTTTCATAG AAAAGCTCCA CCAAGCAGCA 
     2641 TTTTATGTGG AACAGAGAAG CCAGAAGTAT ACATTGAACC TTGTAATTCT GTCATTGTTC 
     2701 AGATTAAAGC AGCAGAGATC GTACCCAGTG ATATGTATAA AACTGGCTGC ACCTTGCGTT 
     2761 TTCCACGAAT TGAAAAGATA AGAGATGACA AGGAGTGGCA TGAGTGCATG ACCCTGGACG 
     2821 ACCTAGAACA ACTTAGGGGG AAGGCATCTG GTAAGCTCGC ATCTAAACAC CTTTATATAG 
     2881 GTGGTGATGA TGAACCACAA GAAAAAAAGC GGAAAGCTGC CCCAAAGATG AAGAAAGTTA 
     2941 TTGGAATTAT TGAGCACTTA AAAGCACCTA ACCTTACTAA CGTTAACAAA ATTTCTAATA 
     3001 TATTTGAAGA TGTAGAGTTT TGTGTTATGA GTGGAACAGA TAGCCAGCCA AAGCCTGACC 
     3061 TGGAGAACAG AATTGCAGAA TTTGGTGGTT ATATAGTACA AAATCCAGGC CCAGACACGT 
     3121 ACTGTGTAAT TGCAGGGTCT GAGAACATCA GAGTGAAAAA CATAATTTTG TCAAATAAAC 
     3181 ATGATGTTGT CAAGCCTGCA TGGCTTTTAG AATGTTTTAA GACCAAAAGC TTTGTACCAT 
     3241 GGCAGCCTCG CTTTATGATT CATATGTGCC CATCAACCAA AGAACATTTT GCCCGTGAAT 
     3301 ATGATTGCTA TGGTGATAGT TATTTCATTG ATACAGACTT GAACCAACTG AAGGAAGTAT 
     3361 TCTCAGGAAT TAAAAATTCT AACGAGCAGA CTCCTGAAGA AATGGCTTCT CTGATTGCTG 
     3421 ATTTAGAATA TCGGTATTCC TGGGATTGCT CTCCTCTCAG TATGTTTCGA CGCCACACCG 
     3481 TTTATTTGGA CTCGTATGCT GTTATTAATG ACCTGAGTAC CAAAAATGAG GGGACAAGGT 
     3541 TAGCTATTAA AGCCTTGGAG CTTCGGTTTC ATGGAGCAAA AGTAGTTTCT TGTTTAGCTG 
     3601 AGGGAGTGTC TCATGTAATA ATTGGGGAAG ATCATAGTCG TGTTGCAGAT TTTAAAGCTT 
     3661 TTAGAAGAAC TTTTAAGAGA AAGTTTAAAA TCCTAAAAGA AAGTTGGGTA ACTGATTCAA 
     3721 TAGACAAGTG TGAATTACAA GAAGAAAACC AGTATTTGAT TACGCGTACG CGGCCGCTCG 
     3781 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     3841 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     3901 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     3961 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     4021 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     4081 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     4141 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     4201 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     4261 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     4321 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     4381 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     4441 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     4501 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     4561 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     4621 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     4681 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     4741 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     4801 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     4861 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     4921 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     4981 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     5041 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     5101 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     5161 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     5221 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     5281 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     5341 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     5401 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     5461 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     5521 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     5581 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     5641 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     5701 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     5761 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     5821 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     5881 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     5941 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     6001 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     6061 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     6121 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     6181 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     6241 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     6301 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     6361 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     6421 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     6481 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     6541 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     6601 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     6661 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     6721 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     6781 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     6841 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     6901 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     6961 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     7021 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     7081 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     7141 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     7201 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     7261 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     7321 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     7381 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     7441 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     7501 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     7561 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
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