LOCUS       RC224530                7932 bp    DNA     circular  SYN 
DEFINITION  GPR64 (Myc-DDK-tagged)-Human G protein-coupled receptor 64 (GPR64), transcript variant 1
ACCESSION   NM_001079858
VERSION     NM_001079858.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4089..4180 
                     /label="Myc-DDK"  
     misc_feature    4210..4795 
                     /label="PolyA signal"  
     misc_feature    4267..4286 
                     /label="Restriction Enzyme"  
     misc_feature    5071..5743 
                     /label="ColE1"  
     misc_feature    6173..6967 
                     /label="Kan/Neo"  
     misc_feature    7002..7304 
                     /label="SV40 ori"  
     misc_feature    7390..7867 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4071..4077 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4071 
                     /label="ADGRG2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTTTTCTCT GTCAGGCAGT GTGGCCATGT TGGCAGAACT GAAGAAGTTT 
     1081 TACTGACGTT CAAGATATTC CTTGTCATCA TTTGTCTTCA TGTCGTTCTG GTAACATCCC 
     1141 TGGAAGAAGA TACTGATAAT TCCAGTTTGT CACCACCACC TGCTAAATTA TCTGTTGTCA 
     1201 GTTTTGCCCC CTCCTCCAAT GGTACTCCAG AGGTTGAAAC AACAAGCCTC AATGATGTTA 
     1261 CTTTAAGCTT ACTCCCTTCA AACGAAACAG AAAAAACTAA AATCACTATA GTAAAAACCT 
     1321 TCAATGCATC AGGCGTCAAA CCCCAGAGAA ATATCTGCAA TTTGTCATCT ATTTGCAATG 
     1381 ACTCAGCATT TTTTAGAGGT GAGATCATGT TTCAATATGA TAAAGAAAGC ACTGTTCCCC 
     1441 AGAATCAACA TATAACGAAT GGCACCTTAA CTGGAGTCCT GTCTCTAAGT GAATTAAAAC 
     1501 GCTCAGAGCT CAACAAAACC CTGCAAACCC TAAGTGAGAC TTACTTTATA ATGTGTGCTA 
     1561 CAGCAGAGGC CCAAAGCACA TTAAATTGTA CATTCACAAT AAAACTGAAT AATACAATGA 
     1621 ATGCATGTGC TGTAATAGCT GCTTTGGAAA GAGTAAAGAT TCGACCAATG GAACACTGCT 
     1681 GCTGTTCTGT CAGGATACCC TGCCCTTCCT CCCCAGAAGA GTTGGAAAAG CTTCAGTGTG 
     1741 ACCTGCAGGA TCCCATTGTC TGTCTTGCTG ACCATCCACG TGGCCCACCA TTTTCTTCCA 
     1801 GCCAATCCAT CCCAGTGGTG CCTCGGGCCA CTGTGCTTTC CCAGGTCCCC AAAGCTACCT 
     1861 CTTTTGCTGA GCCTCCAGAT TATTCACCTG TGACCCACAA TGTTCCCTCT CCAATAGGGG 
     1921 AGATTCAACC CCTTTCACCC CAGCCTTCAG CTCCCATAGC TTCCAGCCCT GCCATTGACA 
     1981 TGCCCCCACA GTCTGAAACG ATCTCTTCCC CTATGCCCCA AACCCATGTC TCCGGCACCC 
     2041 CACCTCCTGT GAAAGCCTCA TTTTCCTCTC CCACCGTGTC TGCCCCTGCG AATGTCAACA 
     2101 CTACCAGCGC ACCTCCTGTC CAGACAGACA TCGTCAACAC CAGCAGTATT TCTGATCTTG 
     2161 AGAACCAAGT GTTGCAGATG GAGAAGGCTC TGTCCTTGGG CAGCCTGGAG CCTAACCTCG 
     2221 CAGGAGAAAT GATCAACCAA GTCAGCAGAC TCCTTCATTC CCCGCCTGAC ATGCTGGCCC 
     2281 CTCTGGCTCA AAGATTGCTG AAAGTAGTGG ATGACATTGG CCTACAGCTG AACTTTTCAA 
     2341 ACACGACTAT AAGTCTAACC TCCCCTTCTT TGGCTCTGGC TGTGATCAGA GTGAATGCCA 
     2401 GTAGTTTCAA CACAACTACC TTTGTGGCCC AAGACCCTGC AAATCTTCAG GTTTCTCTGG 
     2461 AAACCCAAGC TCCTGAGAAC AGTATTGGCA CAATTACTCT TCCTTCATCG CTGATGAATA 
     2521 ATTTACCAGC TCATGACATG GAGCTAGCTT CCAGGGTTCA GTTCAATTTT TTTGAAACAC 
     2581 CTGCTTTGTT TCAGGATCCT TCCCTGGAGA ACCTCTCTCT GATCAGCTAC GTCATATCAT 
     2641 CGAGTGTTGC AAACCTGACC GTCAGGAACT TGACAAGAAA CGTGACAGTC ACATTAAAGC 
     2701 ACATCAACCC GAGCCAGGAT GAGTTAACAG TGAGATGTGT ATTTTGGGAC TTGGGCAGAA 
     2761 ATGGTGGCAG AGGAGGCTGG TCAGACAATG GCTGCTCTGT CAAAGACAGG AGATTGAATG 
     2821 AAACCATCTG TACCTGTAGC CATCTAACAA GCTTCGGCGT TCTGCTGGAC CTATCTAGGA 
     2881 CATCTGTGCT GCCTGCTCAA ATGATGGCTC TGACGTTCAT TACATATATT GGTTGTGGGC 
     2941 TTTCATCAAT TTTTCTGTCA GTGACTCTTG TAACCTACAT AGCTTTTGAA AAGATCCGGA 
     3001 GGGATTACCC TTCCAAAATC CTCATCCAGC TGTGTGCTGC TCTGCTTCTG CTGAACCTGG 
     3061 TCTTCCTCCT GGACTCGTGG ATTGCTCTGT ATAAGATGCA AGGCCTCTGC ATCTCAGTGG 
     3121 CTGTATTTCT TCATTATTTT CTCTTGGTCT CATTCACATG GATGGGCCTA GAAGCATTCC 
     3181 ATATGTACCT GGCCCTTGTC AAAGTATTTA ATACTTACAT CCGAAAATAC ATCCTTAAAT 
     3241 TCTGCATTGT CGGTTGGGGG GTACCAGCTG TGGTTGTGAC CATCATCCTG ACTATATCCC 
     3301 CAGATAACTA TGGGCTTGGA TCCTATGGGA AATTCCCCAA TGGTTCACCG GATGACTTCT 
     3361 GCTGGATCAA CAACAATGCA GTATTCTACA TTACGGTGGT GGGATATTTC TGTGTGATAT 
     3421 TTTTGCTGAA CGTCAGCATG TTCATTGTGG TCCTGGTTCA GCTCTGTCGA ATTAAAAAGA 
     3481 AGAAGCAACT GGGAGCCCAG CGAAAAACCA GTATTCAAGA CCTCAGGAGT ATCGCTGGCC 
     3541 TTACATTTTT ACTGGGAATA ACTTGGGGCT TTGCCTTCTT TGCCTGGGGA CCAGTTAACG 
     3601 TGACCTTCAT GTATCTGTTT GCCATCTTTA ATACCTTACA AGGATTTTTC ATATTCATCT 
     3661 TTTACTGTGT GGCCAAAGAA AATGTCAGGA AGCAATGGAG GCGGTATCTT TGTTGTGGAA 
     3721 AGTTACGGCT GGCTGAAAAT TCTGACTGGA GTAAAACTGC TACTAATGGT TTAAAGAAGC 
     3781 AGACTGTAAA CCAAGGAGTG TCCAGCTCTT CAAATTCCTT ACAGTCAAGC AGTAACTCCA 
     3841 CTAACTCCAC CACACTGCTA GTGAATAATG ATTGCTCAGT ACACGCAAGC GGGAATGGAA 
     3901 ATGCTTCTAC AGAGAGGAAT GGGGTCTCTT TTAGTGTTCA GAATGGAGAT GTGTGCCTTC 
     3961 ACGATTTCAC TGGAAAACAG CACATGTTTA ACGAGAAGGA AGATTCCTGC AATGGGAAAG 
     4021 GCCGTATGGC TCTCAGAAGG ACTTCAAAGC GGGGAAGCTT ACACTTTATT GAGCAAATGA 
     4081 CGCGTACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA 
     4141 TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     4201 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     4261 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     4321 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     4381 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     4441 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     4501 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     4561 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     4621 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     4681 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     4741 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     4801 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCGGCGAGC 
     4861 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 
     4921 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 
     4981 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 
     5041 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 
     5101 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 
     5161 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 
     5221 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 
     5281 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 
     5341 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 
     5401 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 
     5461 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 
     5521 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 
     5581 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 
     5641 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 
     5701 TTTTAAATCA ATCTAAAGTA TATATGAGTA ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC 
     5761 GACGTTGGCT GCGAGCCCTG GGCCTTCACC CGAACTTGGG GGGTGGGGTG GGGAAAAGGA 
     5821 AGAAACGCGG GCGTATTGGC CCCAATGGGG TCTCGGTGGG GTATCGACAG AGTGCCAGCC 
     5881 CTGGGACCGA ACCCCGCGTT TATGAACAAA CGACCCAACA CCGTGCGTTT TATTCTGTCT 
     5941 TTTTATTGCC GTCATAGCGC GGGTTCCTTC CGGTATTGTC TCCTTCCGTG TTTCAGTTAG 
     6001 CCTCCCCCTA GGGTGGGCGA AGAACTCCAG CATGAGATCC CCGCGCTGGA GGATCATCCA 
     6061 GCCGGCGTCC CGGAAAACGA TTCCGAAGCC CAACCTTTCA TAGAAGGCGG CGGTGGAATC 
     6121 GAAATCTCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC ATTTCGAACC CCAGAGTCCC 
     6181 GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC GGGAGCGGCG 
     6241 ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC AGCAATATCA 
     6301 CGGGTAGCCA ACGCTATGTC CTGATAGCGA TCCGCCACAC CCAGCCGGCC ACAGTCGATG 
     6361 AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC GCCATGGGTC 
     6421 ACGACGAGAT CCTCGCCGTC GGGCATGCTC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC 
     6481 GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA 
     6541 GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA 
     6601 AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG 
     6661 TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT 
     6721 TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC 
     6781 CGCGCTGCCT CGTCTTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT GACAAAAAGA 
     6841 ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC GATTGTCTGT 
     6901 TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC TGCGTGCAAT 
     6961 CCATCTTGTT CAATCATGCG AAACGATCCT CATCCTGTCT CTTGATCGAT CTTTGCAAAA 
     7021 GCCTAGGCCT CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC 
     7081 CTCGGCCTCT GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGAGAAT GGGCGGAACT 
     7141 GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG ACTATGGTTG CTGACTAATT 
     7201 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCTGGT 
     7261 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7321 TCCACACCCT AACTGACACA CATTCCACAG CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT 
     7381 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 
     7441 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 
     7501 GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC 
     7561 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC 
     7621 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT 
     7681 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG 
     7741 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT 
     7801 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA 
     7861 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT 
     7921 AACGCGAATT TT
//