LOCUS       RC224512                9396 bp    DNA     circular  SYN 
DEFINITION  CUX1 (Myc-DDK-tagged)-Human cut-like homeobox 1 (CUX1), transcript variant 1
ACCESSION   NM_181552
VERSION     NM_181552.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5553..5644 
                     /label="Myc-DDK"  
     misc_feature    5674..6259 
                     /label="PolyA signal"  
     misc_feature    5731..5750 
                     /label="Restriction Enzyme"  
     misc_feature    6535..7207 
                     /label="ColE1"  
     misc_feature    7637..8431 
                     /label="Kan/Neo"  
     misc_feature    8466..8768 
                     /label="SV40 ori"  
     misc_feature    8854..9331 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5535..5541 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5535 
                     /label="CUX1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTTGTGCGTA GCCGGAGCCA GGTTGAAGAG AGAACTCGAT GCCACCGCAA 
     1081 CGGTATTGGC GAACCGGCAG GATGAAAGTG AGCAGTCCAG AAAGCGGCTT ATCGAACAGA 
     1141 GCCGGGAGTT CAAGAAGAAC ACTCCAGAGG ATTTGCGCAA GCAGGTAGCG CCGCTGCTGA 
     1201 AGAGTTTCCA AGGAGAGATT GATGCACTGA GTAAAAGAAG CAAGGAAGCT GAAGCAGCTT 
     1261 TCTTGAATGT CTACAAAAGA TTGATTGACG TCCCAGATCC CGTACCAGCT TTGGATCTCG 
     1321 GACAGCAACT CCAGCTCAAA GTGCAGCGCC TGCACGATAT TGAAACAGAG AACCAGAAAC 
     1381 TTAGGGAAAC TCTGGAAGAA TACAACAAGG AATTTGCTGA AGTGAAAAAT CAAGAGGTTA 
     1441 CGATAAAAGC ACTTAAAGAG AAAATCCGAG AATATGAACA GACACTGAAG AACCAAGCCG 
     1501 AAACCATAGC TCTTGAGAAG GAACAGAAGT TACAGAATGA CTTTGCAGAA AAGGAGAGAA 
     1561 AGCTGCAGGA GACACAGATG TCCACCACCT CAAAGCTGGA GGAAGCTGAG CATAAGGTTC 
     1621 AGAGCCTACA AACAGCCCTG GAAAAAACTC GAACAGAATT ATTTGACCTG AAAACCAAAT 
     1681 ACGATGAAGA AACTACTGCA AAGGCCGACG AGATTGAAAT GATCATGACG GACCTTGAAA 
     1741 GGGCAAACCA GAGGGCAGAG GTGGCTCAGA GAGAGGCGGA GACCTTAAGG GAACAGCTCT 
     1801 CATCGGCCAA TCACTCCCTC CAGCTGGCCT CACAGATCCA GAAGGCACCA GACGTGGAGC 
     1861 AGGCCATAGA GGTGCTGACC CGCTCCAGCC TAGAAGTTGA GTTGGCCGCC AAGGAGCGGG 
     1921 AGATCGCACA GCTGGTGGAG GACGTGCAGA GACTCCAGGC CAGCCTCACC AAGCTGCGGG 
     1981 AGAATTCGGC CAGCCAGATC TCACAGCTTG AGCAGCAGCT GAGCGCCAAA AACAGCACAC 
     2041 TCAAACAACT GGAAGAAAAA CTCAAAGGCC AGGCTGACTA TGAAGAGGTG AAGAAAGAGC 
     2101 TGAACATTCT GAAGTCCATG GAGTTTGCAC CGTCCGAGGG CGCTGGGACA CAGGATGCGG 
     2161 CCAAGCCCCT GGAGGTGCTG TTGCTGGAGA AGAACCGCTC GCTGCAGTCC GAGAACGCCG 
     2221 CGCTGCGCAT CTCCAACAGC GACCTGAGCG GGTCAGCCAG GAGGAAAGGG AAAGACCAGC 
     2281 CTGAAAGTCG GCGCCCGGGA TCTTTGCCGG CCCCCCCTCC TTCTCAGTTG CCCCGCAACC 
     2341 CGGGGGAGCA GGCTTCCAAT ACTAATGGTA CACACCAGTT CTCACCAGCG GGGTTAAGTC 
     2401 AAGACTTTTT CAGCTCATCC CTGGCAAGCC CCAGCCTACC CCTGGCTTCT ACAGGAAAAT 
     2461 TTGCACTAAA CTCTCTTCTC CAGCGGCAGC TAATGCAGTC CTTCTACTCC AAGGCTATGC 
     2521 AGGAAGCCGG AAGCACAAGC ATGATTTTTT CAACAGGTCC ATACAGCACA AACTCCATAT 
     2581 CTTCCCAAAG TCCATTACAA CAAAGCCCAG ATGTCAATGG CATGGCCCCA TCCCCCAGCC 
     2641 AGTCAGAAAG TGCTGGGAGC GTCTCCGAGG GCGAGGAGAT GGACACTGCA GAAATCGCCC 
     2701 GGCAGGTCAA AGAGCAGCTG ATTAAGCACA ATATCGGACA ACGTATTTTC GGACATTATG 
     2761 TGTTGGGACT GTCTCAAGGG TCCGTGAGCG AGATTCTGGC CCGGCCCAAG CCATGGAATA 
     2821 AACTGACTGT TCGTGGCAAG GAGCCATTTC ACAAGATGAA ACAGTTCCTC TCCGATGAGC 
     2881 AGAACATCCT GGCCCTCCGT AGCATCCAAG GCAGACAAAG AGAGAATCCA GGCCAGAGCC 
     2941 TGAACAGACT ATTTCAGGAA GTACCGAAAC GAAGAAATGG GTCTGAAGGT AACATCACCA 
     3001 CCCGGATCCG AGCCTCGGAG ACTGGCTCTG ATGAAGCCAT CAAGTCCATC CTAGAGCAAG 
     3061 CCAAGAGGGA GCTCCAAGTG CAGAAAACTG CAGAGCCGGC CCAGCCTTCC TCCGCATCCG 
     3121 GCAGCGGGAA CTCTGATGAC GCCATCCGCT CCATCCTGCA GCAAGCCCGC CGGGAGATGG 
     3181 AGGCCCAGCA GGCTGCCCTC GACCCTGCCT TAAAGCAGGC ACCACTGTCC CAGAGTGACA 
     3241 TCACCATCCT CACCCCCAAG CTTCTGTCCA CCTCGCCCAT GCCCACCGTG TCCAGCTACC 
     3301 CACCTCTCGC CATCTCCCTG AAGAAGCCCT CCGCAGCTCC TGAGGCCGGT GCCTCTGCTC 
     3361 TGCCGAACCC CCCGGCCCTC AAAAAGGAGG CCCAGGACGC CCCCGGGCTG GACCCCCAGG 
     3421 GAGCAGCCGA TTGTGCACAA GGGGTCCTGA GACAGGTGAA AAATGAGGTG GGCCGCAGCG 
     3481 GTGCCTGGAA GGACCACTGG TGGAGCGCGG TGCAGCCGGA GAGAAGAAAT GCCGCCTCCT 
     3541 CCGAGGAGGC CAAGGCCGAA GAAACGGGCG GCGGGAAAGA GAAGGGCAGC GGTGGCAGCG 
     3601 GAGGTGGCAG CCAGCCTCGG GCCGAGCGCA GTCAGCTCCA GGGACCCTCG TCGTCAGAGT 
     3661 ACTGGAAGGA GTGGCCCAGC GCTGAGTCCC CATACTCCCA GAGCTCAGAG CTGAGTCTGA 
     3721 CCGGGGCCAG CCGCAGCGAG ACACCACAGA ACAGCCCCCT GCCATCCTCC CCGATCGTGC 
     3781 CCATGTCCAA GCCCACCAAG CCCTCGGTCC CCCCGCTGAC CCCCGAGCAG TACGAGGTCT 
     3841 ACATGTACCA GGAGGTGGAC ACCATCGAGC TCACCCGGCA GGTTAAGGAA AAGCTGGCCA 
     3901 AGAACGGCAT CTGCCAGAGA ATCTTCGGGG AGAAGGTGCT GGGCCTGTCC CAGGGCAGCG 
     3961 TCAGCGACAT GCTGTCCCGA CCGAAGCCAT GGAGCAAGCT GACGCAGAAA GGCCGAGAAC 
     4021 CCTTCATCCG GATGCAGCTC TGGCTGAACG GCGAGCTAGG CCAGGGTGTT CTACCCGTCC 
     4081 AGGGCCAGCA GCAAGGGCCA GTCCTCCACT CCGTGACATC GCTCCAGGAC CCGCTGCAGC 
     4141 AGGGCTGTGT GAGCTCAGAA AGCACTCCAA AGACCTCCGC CAGCTGCAGC CCTGCCCCTG 
     4201 AGTCCCCGAT GAGTTCCAGT GAGTCGGTGA AGAGCCTGAC CGAGCTGGTC CAGCAGCCCT 
     4261 GTCCCCCCAT CGAGGCGAGC AAGGACAGCA AGCCACCAGA GCCCAGTGAC CCGCCAGCAT 
     4321 CCGACTCCCA GCCCACAACC CCGCTGCCTC TCTCCGGACA CTCGGCCCTC AGCATCCAAG 
     4381 AATTAGTAGC CATGTCCCCG GAGCTGGACA CCTACGGCAT AACCAAGCGG GTGAAGGAGG 
     4441 TGCTGACGGA CAACAACCTC GGCCAGCGCT TATTTGGGGA GACCATCTTA GGGCTCACCC 
     4501 AAGGCTCTGT CTCTGACCTC CTTGCCCGCC CCAAACCCTG GCATAAGCTC AGTCTGAAAG 
     4561 GACGAGAGCC CTTCGTCCGG ATGCAGCTGT GGCTGAACGA CCCCAACAAT GTGGAGAAGC 
     4621 TGATGGACAT GAAACGGATG GAGAAGAAAG CCTACATGAA GCGGCGGCAC AGCTCAGTCA 
     4681 GTGACAGCCA GCCCTGCGAA CCGCCCTCTG TCGGCACCGA GTACAGCCAG GGCGCCAGCC 
     4741 CCCAGCCCCA GCACCAGCTG AAGAAACCCC GGGTGGTGCT GGCTCCGGAG GAGAAGGAGG 
     4801 CGCTGAAACG AGCGTATCAG CAAAAGCCAT ACCCGTCACC AAAAACCATC GAAGACCTCG 
     4861 CCACCCAGCT CAACCTGAAA ACCAGCACCG TCATCAACTG GTTCCACAAC TACAGGTCTC 
     4921 GGATCCGCAG AGAACTGTTC ATTGAGGAAA TTCAGGCCGG GAGTCAGGGC CAGGCGGGCG 
     4981 CCAGCGACTC ACCCTCGGCC CGCAGCGGCC GGGCGGCGCC CAGCTCGGAG GGCGACAGCT 
     5041 GCGACGGCGT GGAGGCCACT GAGGGCCCAG GCAGCGCCGA CACCGAGGAG CCCAAGTCTC 
     5101 AGGGAGAGGC CGAGCGGGAG GAGGTGCCGC GGCCGGCGGA GCAGACGGAG CCGCCGCCCT 
     5161 CGGGGACCCC GGGCCCGGAC GACGCCCGCG ACGACGACCA CGAGGGAGGC CCCGTGGAAG 
     5221 GCCCGGGGCC CCTGCCCAGC CCCGCCTCCG CGACCGCCAC CGCCGCGCCC GCGGCCCCCG 
     5281 AGGACGCCGC TACCTCAGCC GCCGCCGCGC CGGGGGAGGG CCCCGCGGCC CCGAGCTCCG 
     5341 CGCCGCCGCC CAGCAACAGC AGCAGCAGCA GCGCCCCCCG CAGGCCCAGC TCGCTGCAGA 
     5401 GCCTTTTCGG CCTCCCCGAG GCCGCGGGCG CCCGGGACTC GCGCGACAAC CCCCTGCGCA 
     5461 AGAAGAAGGC CGCGAACTTG AACAGCATCA TCCACCGCCT GGAGAAGGCC GCCAGCCGGG 
     5521 AGGAACCTAT CGAATGGGAG TTCACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     5581 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     5641 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     5701 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     5761 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     5821 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     5881 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     5941 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     6001 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     6061 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     6121 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     6181 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     6241 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     6301 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     6361 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     6421 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     6481 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     6541 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     6601 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     6661 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     6721 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     6781 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     6841 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     6901 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     6961 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     7021 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     7081 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     7141 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     7201 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     7261 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     7321 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     7381 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     7441 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     7501 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     7561 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     7621 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     7681 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     7741 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     7801 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     7861 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     7921 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     7981 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     8041 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     8101 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     8161 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     8221 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     8281 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     8341 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     8401 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     8461 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     8521 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     8581 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     8641 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     8701 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     8761 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     8821 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     8881 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     8941 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     9001 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     9061 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     9121 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     9181 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     9241 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     9301 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     9361 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//