LOCUS       RC224431                7689 bp    DNA     circular  SYN 
DEFINITION  AFTPH (Myc-DDK-tagged)-Human aftiphilin (AFTPH), transcript variant 1
ACCESSION   NM_203437
VERSION     NM_203437.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3846..3937 
                     /label="Myc-DDK"  
     misc_feature    3967..4552 
                     /label="PolyA signal"  
     misc_feature    4024..4043 
                     /label="Restriction Enzyme"  
     misc_feature    4828..5500 
                     /label="ColE1"  
     misc_feature    5930..6724 
                     /label="Kan/Neo"  
     misc_feature    6759..7061 
                     /label="SV40 ori"  
     misc_feature    7147..7624 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3828..3834 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3828 
                     /label="AFTPH"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGCCAGAC ATCATTCGAA TGTACTCTTC ATCCCCACCA CCATTAGACA 
     1081 ATGGAGCAGA GGATGATGAT GATGATGAAT TTGGGGAATT TGGTGGGTTT TCAGAAGTTA 
     1141 GCCCTTCTGG TGTAGGGTTT GTTGATTTCG ATACACCAGA TTATACTCGT CCCAAGGAAG 
     1201 AGTTTGTACC TTCAAACCAT TTTATGCCAA TTCATGAATT CTCAGAAAAT GTAGATAGCC 
     1261 TTACAAGCTT TAAGTCCATT AAAAATGGTA ATGATAAGGA CATCACTGCT GAACTTTCTG 
     1321 CTCCTGTGAA AGGACAGTCT GATGTTTTAC TTTCTACCAC CAGCAAAGAA ATAATTTCAT 
     1381 CTGAAATGTT AGCTACTTCC ATTGATGGCA TGGAAAGACC AGGAAATTTA AATAAAGTAG 
     1441 TGGAGCAGAG ACAGAATGTT GGAACACTTG AAAGTTTCTC TCCAGGAGAT TTTAGAACTA 
     1501 ATATGAATGT TGTTCATCAA AACAAGCAGT TAGAGAGCTG CAATGGTGAA AAGCCTCCTT 
     1561 GTCTGGAGAT TCTAACAAAT GGGTTTGCAG TGTTGGAAAC TGTAAATCCT CAGGGAACAG 
     1621 ATGATCTGGA CAATGTAGCT GATTCAAAGG GACGGAAGCC TCTTAGCACT CATAGCACTG 
     1681 AGTATAATTT AGACTCTGTA CCTAGTCCTG CTGAGGAATT TGCAGATTTT GCCACATTTT 
     1741 CCAAAAAGGA AAGGATACAA TTAGAAGAAA TAGAATGTGC AGTTTTAAAT GATAGAGAAG 
     1801 CACTAACCAT TCGGGAAAAC AATAAAATTA ATAGAGTCAA TGAACTGAAT TCTGTAAAAG 
     1861 AAGTGGCTTT GGGTAGAAGC TTGGATAACA AAGGAGACAC TGATGGAGAG GATCAGGTTT 
     1921 GTGTTTCAGA AATAAGCATA GTGACTAACA GAGGTTTCAG TGTTGAAAAA CAAGGCCTTC 
     1981 CAACACTGCA ACAGGATGAA TTTTTACAGT CAGGTGTTCA GTCAAAGGCT TGGAGTTTGG 
     2041 TAGACTCAGC TGATAATTCA GAAGCCATTA GGAGAGAACA ATGTAAAACT GAAGAAAAAC 
     2101 TTGACTTACT TACTTCTAAA TGTGCTCACC TATGCATGGA TTCTGTTAAA ACTTCTGATG 
     2161 ATGAAGTTGG TTCTCCCAAA GAAGAAAGTA GAAAGTTTAC TAATTTCCAA AGCCCAAACA 
     2221 TTGACCCCAC AGAAGAAAAT GATTTGGATG ATTCTTTAAG TGTAAAAAAT GGTGATAGTA 
     2281 GTAATGACTT TGTGACTTGC AATGATATCA ATGAAGATGA TTTTGGTGAT TTTGGTGACT 
     2341 TTGGCTCTGC CAGTGGCTCA ACTCCACCTT TTGTTACTGG TACTCAAGAT TCAATGAGTG 
     2401 ATGCCACTTT TGAAGAGTCT TCAGAGCACT TTCCACATTT TAGTGAACCA GGTGATGACT 
     2461 TTGGAGAATT TGGGGATATA AATGCTGTTT CTTGCCAAGA GGAGACAATA TTAACAAAGT 
     2521 CAGACCTAAA ACAGACTTCT GATAATTTAT CAGAAGAATG TCAATTGGCA AGAAAATCTA 
     2581 GTGGAACAGG CACTGAACCT GTTGCAAAAC TTAAAAATGG GCAAGAAGGT GAGATTGGAC 
     2641 ATTTTGATTC TGTGCCAAAT ATTCAGGATG ACTGCAATGG TTTTCAAGAC TCTGATGATT 
     2701 TTGCAGACTT CAGTTCAGCT GGTCCTAGCC AAGTTGTAGA TTGGAATGCT TTTGAGGATG 
     2761 AACAAAAAGA TAGTTGTTCT TGGGCTGCTT TTGGAGACCA GCAGGCTACT GAATCTCATC 
     2821 ATCGAAAGGA AGCCTGGCAG TCACATAGGA CAGATGAAAA TATTGATACT CCAGGAACCC 
     2881 CCAAAACGCA CAGTGTACCT TCAGCAACTT CCAAAGGAGC AGTTGCTAGT GGCCATTTAC 
     2941 AGGAATCAGC CACTTCAGTT CAGACAGCTT TATTAAACCG CCTGGAGCGA ATTTTCGAAG 
     3001 CATGTTTTCC TTCCATACTT GTCCCTGATG CTGAAGAGGA AGTTACTTCC CTGAAGCACT 
     3061 TGCTGGAAAC AAGCACTTTG CCAATAAAAA CGAGAGAGGC CTTACCTGAA AGTGGGGAAT 
     3121 TGCTAGATGT GTGGACTGAG CTACAGGATA TCCATGATGC ACATGGCTTG AGATACCAGT 
     3181 GGGGCGGCTC CCATAGCAAC AAGAAGCTTT TGTCCTCCTT GGGAATAGAC ACCCGAAACA 
     3241 TTCTCTTCAC GGGCAATAAG AAGCAGCCTG TTATAGTGCC CATGTATGCA GCAGGATTGG 
     3301 GTATGTTAGA GCCCACCAAG GAACCACTGA AACCACTTTC TGCTGCAGAA AAAATAGCTT 
     3361 CCATCGGTCA GACAGCCACC ATGTCACCAG ATATGAACAC ATGTACATCT GATCAGTTCC 
     3421 AGGAGTCTCT ACCACCCGTC CAGTTTGACT GGAGTAGCAG TGGCCTTACT AACCCTTTAG 
     3481 ATGCTAGTGG AGGTTCCACT CTTCTGAACC TTGATTTCTT TGGGCCCGTG GATGACAGTA 
     3541 GCTCTAGCAG CAGCACCACA ATCCCAGGTG TGGATCCGGA GTTGTATGAG TTAACAACTT 
     3601 CTAAGCTGGA AATCTCCACC TCAAGCCTCA AAGTGACTGA TGCATTTGCA AGACTCATGT 
     3661 CTACAGTAGA GAAGACAAGC ACATCTACCA GGAAACCGAA AAGAGAAGAG CACCTAAGTG 
     3721 AAGAAGCTAT CAAGGTGATC GCTGGCCTTC CTGACTTAAC ATTCATGCAT GCCAAGGTGT 
     3781 TGATGTTCCC AGCCACGTTA ACACCTTCCA CAAGCTCTCA AGAAAAAGCA GACGGAACGC 
     3841 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     3901 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     3961 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     4021 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     4081 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     4141 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     4201 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     4261 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     4321 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     4381 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     4441 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     4501 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     4561 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     4621 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     4681 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     4741 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     4801 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     4861 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     4921 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     4981 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     5041 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     5101 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     5161 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     5221 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     5281 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     5341 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     5401 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     5461 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     5521 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     5581 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     5641 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     5701 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     5761 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     5821 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     5881 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     5941 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     6001 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     6061 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     6121 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     6181 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     6241 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     6301 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     6361 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     6421 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     6481 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     6541 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     6601 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     6661 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     6721 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     6781 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     6841 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     6901 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     6961 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     7021 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7081 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     7141 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     7201 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     7261 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     7321 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     7381 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     7441 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     7501 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     7561 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     7621 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     7681 GCGAATTTT
//