LOCUS       RC224406                8895 bp    DNA     circular  SYN 
DEFINITION  FLT1 (Myc-DDK-tagged)-Human fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor) (FLT1), transcript variant 1
ACCESSION   NM_002019
VERSION     NM_002019.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5052..5143 
                     /label="Myc-DDK"  
     misc_feature    5173..5758 
                     /label="PolyA signal"  
     misc_feature    5230..5249 
                     /label="Restriction Enzyme"  
     misc_feature    6034..6706 
                     /label="ColE1"  
     misc_feature    7136..7930 
                     /label="Kan/Neo"  
     misc_feature    7965..8267 
                     /label="SV40 ori"  
     misc_feature    8353..8830 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5034..5040 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5034 
                     /label="FLT1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTCAGCTAC TGGGACACCG GGGTCCTGCT GTGCGCGCTG CTCAGCTGTC 
     1081 TGCTTCTCAC AGGATCTAGT TCAGGTTCAA AATTAAAAGA TCCTGAACTG AGTTTAAAAG 
     1141 GCACCCAGCA CATCATGCAA GCAGGCCAGA CACTGCATCT CCAATGCAGG GGGGAAGCAG 
     1201 CCCATAAATG GTCTTTGCCT GAAATGGTGA GTAAGGAAAG CGAAAGGCTG AGCATAACTA 
     1261 AATCTGCCTG TGGAAGAAAT GGCAAACAAT TCTGCAGTAC TTTAACCTTG AACACAGCTC 
     1321 AAGCAAACCA CACTGGCTTC TACAGCTGCA AATATCTAGC TGTACCTACT TCAAAGAAGA 
     1381 AGGAAACAGA ATCTGCAATC TATATATTTA TTAGTGATAC AGGTAGACCT TTCGTAGAGA 
     1441 TGTACAGTGA AATCCCCGAA ATTATACACA TGACTGAAGG AAGGGAGCTC GTCATTCCCT 
     1501 GCCGGGTTAC GTCACCTAAC ATCACTGTTA CTTTAAAAAA GTTTCCACTT GACACTTTGA 
     1561 TCCCTGATGG AAAACGCATA ATCTGGGACA GTAGAAAGGG CTTCATCATA TCAAATGCAA 
     1621 CGTACAAAGA AATAGGGCTT CTGACCTGTG AAGCAACAGT CAATGGGCAT TTGTATAAGA 
     1681 CAAACTATCT CACACATCGA CAAACCAATA CAATCATAGA TGTCCAAATA AGCACACCAC 
     1741 GCCCAGTCAA ATTACTTAGA GGCCATACTC TTGTCCTCAA TTGTACTGCT ACCACTCCCT 
     1801 TGAACACGAG AGTTCAAATG ACCTGGAGTT ACCCTGATGA AAAAAATAAG AGAGCTTCCG 
     1861 TAAGGCGACG AATTGACCAA AGCAATTCCC ATGCCAACAT ATTCTACAGT GTTCTTACTA 
     1921 TTGACAAAAT GCAGAACAAA GACAAAGGAC TTTATACTTG TCGTGTAAGG AGTGGACCAT 
     1981 CATTCAAATC TGTTAACACC TCAGTGCATA TATATGATAA AGCATTCATC ACTGTGAAAC 
     2041 ATCGAAAACA GCAGGTGCTT GAAACCGTAG CTGGCAAGCG GTCTTACCGG CTCTCTATGA 
     2101 AAGTGAAGGC ATTTCCCTCG CCGGAAGTTG TATGGTTAAA AGATGGGTTA CCTGCGACTG 
     2161 AGAAATCTGC TCGCTATTTG ACTCGTGGCT ACTCGTTAAT TATCAAGGAC GTAACTGAAG 
     2221 AGGATGCAGG GAATTATACA ATCTTGCTGA GCATAAAACA GTCAAATGTG TTTAAAAACC 
     2281 TCACTGCCAC TCTAATTGTC AATGTGAAAC CCCAGATTTA CGAAAAGGCC GTGTCATCGT 
     2341 TTCCAGACCC GGCTCTCTAC CCACTGGGCA GCAGACAAAT CCTGACTTGT ACCGCATATG 
     2401 GTATCCCTCA ACCTACAATC AAGTGGTTCT GGCACCCCTG TAACCATAAT CATTCCGAAG 
     2461 CAAGGTGTGA CTTTTGTTCC AATAATGAAG AGTCCTTTAT CCTGGATGCT GACAGCAACA 
     2521 TGGGAAACAG AATTGAGAGC ATCACTCAGC GCATGGCAAT AATAGAAGGA AAGAATAAGA 
     2581 TGGCTAGCAC CTTGGTTGTG GCTGACTCTA GAATTTCTGG AATCTACATT TGCATAGCTT 
     2641 CCAATAAAGT TGGGACTGTG GGAAGAAACA TAAGCTTTTA TATCACAGAT GTGCCAAATG 
     2701 GGTTTCATGT TAACTTGGAA AAAATGCCGA CGGAAGGAGA GGACCTGAAA CTGTCTTGCA 
     2761 CAGTTAACAA GTTCTTATAC AGAGACGTTA CTTGGATTTT ACTGCGGACA GTTAATAACA 
     2821 GAACAATGCA CTACAGTATT AGCAAGCAAA AAATGGCCAT CACTAAGGAG CACTCCATCA 
     2881 CTCTTAATCT TACCATCATG AATGTTTCCC TGCAAGATTC AGGCACCTAT GCCTGCAGAG 
     2941 CCAGGAATGT ATACACAGGG GAAGAAATCC TCCAGAAGAA AGAAATTACA ATCAGAGATC 
     3001 AGGAAGCACC ATACCTCCTG CGAAACCTCA GTGATCACAC AGTGGCCATC AGCAGTTCCA 
     3061 CCACTTTAGA CTGTCATGCT AATGGTGTCC CCGAGCCTCA GATCACTTGG TTTAAAAACA 
     3121 ACCACAAAAT ACAACAAGAG CCTGGAATTA TTTTAGGACC AGGAAGCAGC ACGCTGTTTA 
     3181 TTGAAAGAGT CACAGAAGAG GATGAAGGTG TCTATCACTG CAAAGCCACC AACCAGAAGG 
     3241 GCTCTGTGGA AAGTTCAGCA TACCTCACTG TTCAAGGAAC CTCGGACAAG TCTAATCTGG 
     3301 AGCTGATCAC TCTAACATGC ACCTGTGTGG CTGCGACTCT CTTCTGGCTC CTATTAACCC 
     3361 TCTTTATCCG AAAAATGAAA AGGTCTTCTT CTGAAATAAA GACTGACTAC CTATCAATTA 
     3421 TAATGGACCC AGATGAAGTT CCTTTGGATG AGCAGTGTGA GCGGCTCCCT TATGATGCCA 
     3481 GCAAGTGGGA GTTTGCCCGG GAGAGACTTA AACTGGGCAA ATCACTTGGA AGAGGGGCTT 
     3541 TTGGAAAAGT GGTTCAAGCA TCAGCATTTG GCATTAAGAA ATCACCTACG TGCCGGACTG 
     3601 TGGCTGTGAA AATGCTGAAA GAGGGGGCCA CGGCCAGCGA GTACAAAGCT CTGATGACTG 
     3661 AGCTAAAAAT CTTGACCCAC ATTGGCCACC ATCTGAACGT GGTTAACCTG CTGGGAGCCT 
     3721 GCACCAAGCA AGGAGGGCCT CTGATGGTGA TTGTTGAATA CTGCAAATAT GGAAATCTCT 
     3781 CCAACTACCT CAAGAGCAAA CGTGACTTAT TTTTTCTCAA CAAGGATGCA GCACTACACA 
     3841 TGGAGCCTAA GAAAGAAAAA ATGGAGCCAG GCCTGGAACA AGGCAAGAAA CCAAGACTAG 
     3901 ATAGCGTCAC CAGCAGCGAA AGCTTTGCGA GCTCCGGCTT TCAGGAAGAT AAAAGTCTGA 
     3961 GTGATGTTGA GGAAGAGGAG GATTCTGACG GTTTCTACAA GGAGCCCATC ACTATGGAAG 
     4021 ATCTGATTTC TTACAGTTTT CAAGTGGCCA GAGGCATGGA GTTCCTGTCT TCCAGAAAGT 
     4081 GCATTCATCG GGACCTGGCA GCGAGAAACA TTCTTTTATC TGAGAACAAC GTGGTGAAGA 
     4141 TTTGTGATTT TGGCCTTGCC CGGGATATTT ATAAGAACCC CGATTATGTG AGAAAAGGAG 
     4201 ATACTCGACT TCCTCTGAAA TGGATGGCTC CCGAATCTAT CTTTGACAAA ATCTACAGCA 
     4261 CCAAGAGCGA CGTGTGGTCT TACGGAGTAT TGCTGTGGGA AATCTTCTCC TTAGGTGGGT 
     4321 CTCCATACCC AGGAGTACAA ATGGATGAGG ACTTTTGCAG TCGCCTGAGG GAAGGCATGA 
     4381 GGATGAGAGC TCCTGAGTAC TCTACTCCTG AAATCTATCA GATCATGCTG GACTGCTGGC 
     4441 ACAGAGACCC AAAAGAAAGG CCAAGATTTG CAGAACTTGT GGAAAAACTA GGTGATTTGC 
     4501 TTCAAGCAAA TGTACAACAG GATGGTAAAG ACTACATCCC AATCAATGCC ATACTGACAG 
     4561 GAAATAGTGG GTTTACATAC TCAACTCCTG CCTTCTCTGA GGACTTCTTC AAGGAAAGTA 
     4621 TTTCAGCTCC GAAGTTTAAT TCAGGAAGCT CTGATGATGT CAGATATGTA AATGCTTTCA 
     4681 AGTTCATGAG CCTGGAAAGA ATCAAAACCT TTGAAGAACT TTTACCGAAT GCCACCTCCA 
     4741 TGTTTGATGA CTACCAGGGC GACAGCAGCA CTCTGTTGGC CTCTCCCATG CTGAAGCGCT 
     4801 TCACCTGGAC TGACAGCAAA CCCAAGGCCT CGCTCAAGAT TGACTTGAGA GTAACCAGTA 
     4861 AAAGTAAGGA GTCGGGGCTG TCTGATGTCA GCAGGCCCAG TTTCTGCCAT TCCAGCTGTG 
     4921 GGCACGTCAG CGAAGGCAAG CGCAGGTTCA CCTACGACCA CGCTGAGCTG GAAAGGAAAA 
     4981 TCGCGTGCTG CTCCCCGCCC CCAGACTACA ACTCGGTGGT CCTGTACTCC ACCCCACCCA 
     5041 TCACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     5101 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     5161 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     5221 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     5281 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     5341 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     5401 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     5461 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     5521 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     5581 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     5641 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     5701 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     5761 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     5821 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     5881 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     5941 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     6001 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     6061 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     6121 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     6181 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     6241 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     6301 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     6361 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     6421 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     6481 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     6541 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     6601 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     6661 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     6721 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     6781 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     6841 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     6901 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     6961 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     7021 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     7081 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     7141 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     7201 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     7261 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     7321 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     7381 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     7441 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     7501 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     7561 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     7621 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     7681 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     7741 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     7801 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     7861 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     7921 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     7981 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     8041 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     8101 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     8161 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     8221 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     8281 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     8341 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     8401 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     8461 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     8521 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     8581 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     8641 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     8701 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     8761 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     8821 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     8881 TTTAACGCGA ATTTT
//