LOCUS       RC224321                8244 bp    DNA     circular  SYN 
DEFINITION  MYT1 (Myc-DDK-tagged)-Human myelin transcription factor 1 (MYT1). Note: ORF is codon optimized
ACCESSION   NM_004535
VERSION     NM_004535.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4401..4492 
                     /label="Myc-DDK"  
     misc_feature    4522..5107 
                     /label="PolyA signal"  
     misc_feature    4579..4598 
                     /label="Restriction Enzyme"  
     misc_feature    5383..6055 
                     /label="ColE1"  
     misc_feature    6485..7279 
                     /label="Kan/Neo"  
     misc_feature    7314..7616 
                     /label="SV40 ori"  
     misc_feature    7702..8179 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4383..4389 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4383 
                     /label="MYT1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCACTTGAG AACGAGGATA AACGCGCCCG CACACGGTCC AAAGCACTGA 
     1081 GAGGGCCACC TGAAACGACT GCTGCAGACC TTTCATGCCC CACCCCTGGC TGCACAGGTA 
     1141 GCGGACATGT GCGCGGAAAG TACTCTAGGC ACCGCTCACT GCAGAGTTGC CCCCTGGCCA 
     1201 AGAAACGCAA GCTTGAGGGC GCCGAGGCTG AACACCTTGT TTCAAAGCGG AAGTCCCACC 
     1261 CACTCAAACT GGCACTGGAT GAAGGTTATG GAGTGGATAG CGACGGGTCT GAGGATACAG 
     1321 AGGTGAAAGA TGCCAGCGTG AGCGACGAAT CCGAGGGAAC GCTGGAGGGT GCTGAAGCCG 
     1381 AAACCTCTGG ACAAGACGAA ATCCACCGGC CAGAGACGGC CGAGGGCAGA TCACCGGTTA 
     1441 AGTCTCACTT CGGCTCAAAT CCTATAGGAT CTGCAACGGC GTCTAGTAAA GGTTCCTATT 
     1501 CTTCTTACCA GGGCATAATT GCTACCTCCC TGTTGAACTT GGGACAGATC GCAGAAGAGA 
     1561 CTCTGGTAGA AGAGGACCTC GGTCAGGCAG CGAAACCCGG ACCTGGTATT GTGCACCTCC 
     1621 TGCAGGAGGC CGCCGAGGGT GCTGCAAGTG AGGAGGGGGA AAAAGGACTG TTTATCCAGC 
     1681 CCGAGGACGC TGAGGAGGTC GTGGAGGTGA CAACAGAACG GAGTCAGGAC CTCTGCCCTC 
     1741 AGAGTCTCGA GGATGCAGCG TCCGAGGAAT CCTCCAAGCA GAAGGGCATC TTGTCTCATG 
     1801 AGGAAGAGGA TGAGGAGGAA GAGGAAGAAG AGGAAGAAGA AGAGGAGGAC GAAGAGGAAG 
     1861 AGGAGGAGGA GGAGGAAGAA GAAGAGGAGG AAGAGGAGGA GGAGGAGGAA GAAGAAGAGG 
     1921 AGGAGGAGGA GGAGGAGGAG GAGGAGGCTG CCCCTGATGT GATTTTTCAG GAAGATACCA 
     1981 GCCACACCTC TGCTCAAAAG GCCCCAGAAC TCCGGGGTCC CGAATCACCC TCTCCTAAGC 
     2041 CCGAGTACTC AGTAATCGTG GAAGTGCGCT CAGATGACGA CAAGGACGAA GATACTCATA 
     2101 GTCGGAAAAG CACAGTGACC GACGAGAGCG AAATGCAGGA TATGATGACC AGGGGAAACC 
     2161 TTGGCTTGCT GGAGCAGGCC ATAGCCCTTA AGGCCGAACA GGTGAGGACT GTCTGCGAGC 
     2221 CGGGCTGTCC TCCTGCCGAA CAGTCACAAC TGGGCCTGGG GGAACCCGGC AAAGCGGCAA 
     2281 AGCCTCTCGA CACGGTTAGG AAGAGCTATT ACTCAAAGGA TCCATCCCGC GCCGAGAAGC 
     2341 GAGAAATTAA ATGTCCCACA CCCGGGTGCG ATGGTACGGG GCATGTTACA GGCCTGTACC 
     2401 CACACCATCG ATCCCTGTCT GGGTGCCCCC ACAAGGATAG AATCCCCCCA GAGATTCTGG 
     2461 CAATGCATGA GAACGTTCTG AAGTGTCCTA CCCCAGGTTG CACCGGACAG GGGCATGTAA 
     2521 ACAGCAACCG GAACACACAT CGGTCACTCT CAGGATGCCC CATTGCAGCG GCTGAGAAAC 
     2581 TTGCGAAAAG CCACGAAAAG CAGCAACCTC AGACGGGGGA CCCGTCAAAA AGCAGCTCTA 
     2641 ATAGTGACAG GATCCTGCGG CCCATGTGCT TCGTCAAGCA GCTGGAGGTG CCCCCATACG 
     2701 GGAGTTACCG GCCAAACGTG GCACCAGCCA CGCCCAGGGC CAACCTTGCC AAAGAGCTGG 
     2761 AAAAGTTCAG CAAAGTCACC TTTGATTATG CTTCATTCGA TGCACAGGTG TTCGGTAAGC 
     2821 GGATGCTCGC TCCTAAGATC CAGACATCTG AGACATCACC CAAAGCTTTT CAGTGTTTCG 
     2881 ATTATTCCCA GGATGCAGAA GCAGCTCATA TGGCTGCAAC AGCTATACTG AATCTGAGTA 
     2941 CGAGGTGCTG GGAGATGCCA GAAAACTTGT CAACAAAGCC TCAGGACTTG CCCAGTAAAT 
     3001 CCGTAGACAT CGAAGTGGAT GAGAATGGTA CGCTGGATTT GTCAATGCAC AAGCATAGAA 
     3061 AAAGAGAGAA TGCGTTCCCA AGCTCAAGCA GTTGCTCTAG CTCACCAGGG GTTAAGTCCC 
     3121 CAGACGCAAG TCAGCGCCAT AGTTCCACCT CCGCCCCTAG CTCATCAATG ACAAGTCCTC 
     3181 AGTCTAGCCA GGCGTCCAGA CAGGACGAGT GGGACAGGCC ACTGGACTAT ACGAAGCCCA 
     3241 GCCGGCTCCG GGAAGAAGAA CCAGAGGAGT CTGAGCCCGC GGCCCATAGT TTTGCCTCAT 
     3301 CCGAGGCAGA TGACCAGGAA GTGTCCGAAG AAAACTTTGA GGAGCGAAAA TATCCGGGTG 
     3361 AGGTTACCCT CACCAATTTC AAGCTCAAGT TCCTGTCTAA GGACATCAAA AAAGAGCTGC 
     3421 TCACATGCCC CACGCCTGGC TGCGATGGAA GCGGGCATAT AACCGGGAAC TATGCAAGCC 
     3481 ATAGATCACT CAGTGGCTGT CCCCTGGCCG ACAAAAGTCT CAGAAATCTG ATGGCCGCCC 
     3541 ACAGCGCGGA TCTCAAGTGC CCAACCCCAG GATGCGACGG AAGTGGCCAC ATCACTGGGA 
     3601 ATTACGCCTC CCACCGGAGC CTCAGCGGGT GCCCAAGGGC TAAAAAAAGC GGAGTAAAGG 
     3661 TGGCTCCCAC TAAGGATGAC AAGGAGGATC CCGAGCTGAT GAAATGCCCT GTTCCAGGCT 
     3721 GTGTTGGGCT TGGCCATATC TCCGGGAAGT ACGCTAGTCA CCGCTCTGCC AGCGGATGCC 
     3781 CCCTGGCTGC TAGGCGCCAG AAAGAGGGGT CCCTGAACGG AAGTTCCTTC TCCTGGAAGA 
     3841 GCCTGAAAAA TGAGGGCCCT ACGTGTCCTA CGCCTGGATG TGATGGATCA GGCCACGCAA 
     3901 ATGGCAGCTT TTTGACACAC CGCTCTCTTT CCGGGTGCCC CAGGGCAACC TTTGCCGGGA 
     3961 AAAAGGGAAA ACTTAGCGGG GATGAAGTTC TCTCTCCTAA ATTCAAGACA TCCGACGTGC 
     4021 TGGAGAATGA TGAAGAAATC AAGCAGCTGA ATCAGGAGAT TCGAGATTTG AACGAAAGCA 
     4081 ACTCAGAAAT GGAGGCTGCC ATGGTGCAGC TCCAGAGCCA GATTAGCAGC ATGGAGAAGA 
     4141 ACCTCAAGAA TATAGAAGAA GAAAACAAGC TTATAGAGGA GCAAAACGAG GCCCTGTTTC 
     4201 TGGAGTTGTC AGGCCTCAGC CAGGCACTGA TTCAGTCCCT GGCTAATATT AGGCTTCCGC 
     4261 ACATGGAACC AATTTGTGAG CAAAACTTTG ATGCATACGT CTCCACGCTT ACAGATATGT 
     4321 ATTCTAACCA GGACCCCGAG AACAAAGACC TCCTGGAATC AATCAAACAG GCCGTGAGGG 
     4381 GAATTCAGGT CACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     4441 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     4501 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     4561 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     4621 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     4681 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     4741 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     4801 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     4861 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     4921 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     4981 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     5041 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     5101 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     5161 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     5221 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     5281 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     5341 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     5401 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     5461 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     5521 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     5581 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     5641 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     5701 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     5761 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     5821 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     5881 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     5941 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     6001 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     6061 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     6121 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     6181 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     6241 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     6301 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     6361 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     6421 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     6481 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     6541 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     6601 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     6661 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     6721 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     6781 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     6841 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     6901 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     6961 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     7021 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     7081 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     7141 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     7201 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     7261 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     7321 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     7381 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     7441 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     7501 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7561 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7621 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     7681 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     7741 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     7801 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     7861 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     7921 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     7981 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     8041 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     8101 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     8161 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     8221 TAACAAAAAT TTAACGCGAA TTTT
//