LOCUS       RC224293                8331 bp    DNA     circular  SYN 
DEFINITION  CCAR1 (Myc-DDK-tagged)-Human cell division cycle and apoptosis regulator 1 (CCAR1)
ACCESSION   NM_018237
VERSION     NM_018237.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4488..4579 
                     /label="Myc-DDK"  
     misc_feature    4609..5194 
                     /label="PolyA signal"  
     misc_feature    4666..4685 
                     /label="Restriction Enzyme"  
     misc_feature    5470..6142 
                     /label="ColE1"  
     misc_feature    6572..7366 
                     /label="Kan/Neo"  
     misc_feature    7401..7703 
                     /label="SV40 ori"  
     misc_feature    7789..8266 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4470..4476 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4470 
                     /label="CCAR1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTCAATTT GGAGGACAGA AGAATCCGCC ATGGGCTACT CAGTTTACAG 
     1081 CCACTGCAGT ATCACAGCCA GCTGCACTGG GTGTTCAACA GCCATCACTC CTTGGAGCAT 
     1141 CTCCTACCAT TTATACACAG CAAACTGCAT TGGCAGCAGC AGGCCTTACC ACACAAACTC 
     1201 CAGCAAACTA TCAGTTAACA CAAACTGCTG CATTGCAGCA ACAAGCCGCA GCTGCAGCAG 
     1261 CTGCATTACA ACAGCAATAT TCACAACCTC AGCAGGCCCT GTATAGTGTG CAACAACAGT 
     1321 TACAGCAACC CCAGCAAACC CTCTTAACAC AGCCAGCTGT TGCACTGCCT ACAAGCCTTA 
     1381 GCCTGTCTAC TCCTCAGCCA ACAGCACAAA TAACTGTATC ATATCCAACA CCAAGGTCCA 
     1441 GTCAACAGCA AACCCAGCCT CAGAAGCAGC GTGTTTTCAC AGGGGTGGTT ACAAAACTAC 
     1501 ATGATACGTT TGGATTTGTG GATGAAGATG TATTCTTTCA GCTTAGTGCT GTCAAAGGGA 
     1561 AAACCCCCCA AGTAGGTGAC AGAGTATTGG TTGAAGCTAC TTATAATCCT AATATGCCTT 
     1621 TTAAATGGAA TGCACAGAGA ATTCAAACAC TACCAAATCA GAATCAGTCG CAAACCCAGC 
     1681 CATTACTGAA GACTCCTCCT GCTGTACTTC AGCCAATTGC ACCACAGACA ACATTTGGTG 
     1741 TTCAGACTCA GCCCCAGCCC CAGTCACTGC TGCAGGCACA GATTTCAGCA GCTTCTATTA 
     1801 CACCACTATT GCAGACTCAA CCACAGCCCT TATTACAGCA GCCTCAGCAA AAAGCTGGTT 
     1861 TATTGCAGCC TCCTGTTCGT ATAGTTTCAC AGCCACAACC GGCACGACGA TTAGATCCCC 
     1921 CATCCCGATT TTCAGGAAGA AATGACAGAG GGGATCAAGT GCCTAACAGA AAAGATGATC 
     1981 GAAGTCGTGA GAGAGAGAGA GAAAGACGTA GATCGAGAGA AAGATCACCT CAGAGGAAAC 
     2041 GTTCCCGGGA AAGATCTCCA CGAAGAGAGC GAGAGCGATC ACCTCGGAGA GTTCGACGTG 
     2101 TTGTTCCACG TTACACAGTT CAGTTTTCAA AGTTTTCTTT AGATTGTCCC AGTTGTGACA 
     2161 TGATGGAACT AAGGCGCCGT TATCAAAATT TGTATATACC TAGTGACTTT TTTGATGCTC 
     2221 AATTTACATG GGTGGATGCT TTCCCTTTGT CAAGACCATT TCAGCTGGGA AATTACTGCA 
     2281 ATTTTTATGT AATGCACAGA GAAGTAGAGT CCTTAGAAAA AAATATGGCC ATTCTTGATC 
     2341 CACCAGATGC TGACCACTTA TACAGTGCAA AGGTAATGCT GATGGCTAGC CCTAGTATGG 
     2401 AAGATTTATA TCATAAGTCA TGTGCTCTTG CTGAGGACCC ACAAGAACTT CGAGATGGAT 
     2461 TCCAACATCC TGCTAGACTT GTTAAGTTTT TAGTGGGCAT GAAAGGCAAG GATGAAGCTA 
     2521 TGGCCATTGG AGGCCACTGG TCTCCTTCGT TGGATGGACC AGACCCAGAA AAAGATCCCT 
     2581 CTGTGTTGAT TAAGACTGCT ATTCGTTGTT GTAAGGCTCT GACAGGCATT GATCTAAGTG 
     2641 TGTGCACACA ATGGTACCGT TTTGCAGAGA TTCGCTACCA TCGCCCTGAG GAAACCCACA 
     2701 AGGGGCGTAC AGTTCCAGCT CATGTGGAGA CAGTGGTTTT ATTTTTCCCG GATGTTTGGC 
     2761 ATTGCCTTCC CACCCGCTCA GAGTGGGAAA CCCTCTCCCG AGGATACAAG CAGCAGCTGG 
     2821 TCGAGAAGCT TCAGGGTGAA CGCAAGGAGG CTGATGGAGA ACAGGATGAA GAAGAGAAGG 
     2881 ATGATGGTGA AGCTAAAGAA ATTTCTACAC CTACCCATTG GTCTAAACTT GATCCAAAGA 
     2941 CAATGAAGGT AAATGACCTC CGAAAAGAAT TAGAAAGTCG AGCTCTTAGT TCCAAAGGAT 
     3001 TAAAATCCCA GTTAATAGCC CGATTGACAA AACAGCTTAA AGTAGAGGAA CAAAAAGAAG 
     3061 AACAGAAGGA GTTAGAGAAA TCTGAAAAAG AAGAGGATGA GGATGATGAT AGGAAATCTG 
     3121 AAGACGATAA AGAGGAAGAA GAAAGGAAAC GTCAAGAGGA AATAGAACGC CAGCGTCGAG 
     3181 AAAGAAGATA TATTTTGCCT GATGAACCGG CCATCATTGT ACATCCAAAT TGGGCTGCAA 
     3241 AAAGTGGCAA GTTTGATTGT AGCATCATGT CTTTGAGTGT CCTATTGGAC TACAGATTAG 
     3301 AGGATAATAA AGAACATTCA TTTGAGGTTT CATTGTTTGC GGAACTTTTC AACGAAATGC 
     3361 TTCAAAGAGA TTTTGGTGTC CGTATATACA AATCATTACT GTCTCTTCCT GAGAAAGAGG 
     3421 ACAAAAAAGA AAAGGATAAA AAAAGCAAAA AAGATGAGAG AAAAGATAAA AAAGAAGAAA 
     3481 GAGATGATGA AACTGATGAA CCAAAACCCA AACGGAGAAA ATCAGGCGAT GATAAAGATA 
     3541 AAAAAGAAGA TAGAGATGAA AGGAAGAAAG AAGATAAAAG AAAAGATGAT TCTAAAGATG 
     3601 ATGATGAAAC TGAAGAAGAT AACAATCAAG ATGAATATGA CCCTATGGAA GCAGAAGAAG 
     3661 CTGAGGATGA AGAAGATGAT AGGGATGAGG AAGAAATGAC CAAACGAGAT GACAAAAGAG 
     3721 ATATCAACAG ATACTGCAAG GAGAGGCCCT CTAAAGATAA GGAAAAAGAA AAGACTCAAA 
     3781 TGATCACAAT TAACAGAGAT CTGTTAATGG CTTTTGTTTA TTTTGATCAA AGTCATTGTG 
     3841 GTTACCTTCT TGAAAAGGAT TTGGAAGAAA TACTTTATAC TCTTGGACTA CATCTTTCTC 
     3901 GGGCTCAGGT AAAGAAGCTT CTTAATAAAG TAGTGCTCCG TGAATCTTGC TTTTACCGGA 
     3961 AATTAACAGA CACCTCAAAA GATGAAGAGA ACCATGAAGA GTCTGAGTCA TTGCAGGAAG 
     4021 ATATGCTAGG AAACAGATTA TTACTTCCAA CACCAACAGT AAAGCAGGAA TCAAAGGATG 
     4081 TGGAAGAAAA TGTTGGCCTC ATTGTGTACA ATGGTGCAAT GGTAGATGTA GGAAGCCTCT 
     4141 TGCAAAAATT GGAAAAGAGC GAAAAAGTAA GAGCTGAGGT AGAACAGAAG CTGCAGTTAC 
     4201 TAGAAGAAAA AACAGATGAA GATGAAAAAA CCATATTAAA TTTGGAGAAT TCCAACAAAA 
     4261 GCCTCTCTGG TGAACTCAGA GAAGTTAAAA AGGACCTTAG TCAGTTACAA GAAAACTTAA 
     4321 AGATTTCGGA AAACATGAAT TTACAATTTG AAAACCAAAT GAATAAGACA ATCAGAAACT 
     4381 TATCTACGGT AATGGATGAA ATCCACACTG TTCTCAAGAA GGATAATGTA AAGAATGAAG 
     4441 ACAAAGATCA AAAATCCAAG GAGAATGGTG CCAGTGTAAC GCGTACGCGG CCGCTCGAGC 
     4501 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     4561 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     4621 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     4681 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     4741 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     4801 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     4861 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     4921 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     4981 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     5041 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     5101 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     5161 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     5221 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     5281 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     5341 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     5401 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     5461 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     5521 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     5581 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     5641 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     5701 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     5761 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     5821 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     5881 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     5941 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     6001 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     6061 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     6121 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     6181 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     6241 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     6301 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     6361 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     6421 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     6481 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     6541 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     6601 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     6661 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     6721 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     6781 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     6841 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     6901 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     6961 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     7021 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     7081 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     7141 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     7201 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     7261 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     7321 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     7381 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     7441 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     7501 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     7561 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7621 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     7681 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     7741 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     7801 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     7861 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     7921 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     7981 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     8041 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     8101 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     8161 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     8221 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     8281 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
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