LOCUS       RC224291                7956 bp    DNA     circular  SYN 
DEFINITION  MPRIP (Myc-DDK-tagged)-Human myosin phosphatase Rho interacting protein (MPRIP), transcript variant 2
ACCESSION   NM_201274
VERSION     NM_201274.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4113..4204 
                     /label="Myc-DDK"  
     misc_feature    4234..4819 
                     /label="PolyA signal"  
     misc_feature    4291..4310 
                     /label="Restriction Enzyme"  
     misc_feature    5095..5767 
                     /label="ColE1"  
     misc_feature    6197..6991 
                     /label="Kan/Neo"  
     misc_feature    7026..7328 
                     /label="SV40 ori"  
     misc_feature    7414..7891 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4095..4101 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4095 
                     /label="MPRIP"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCGGCAGCC AAGGAGAACC CGTGCAGGAA ATTCCAGGCC AACATCTTCA 
     1081 ACAAGAGCAA GTGTCAGAAC TGCTTCAAGC CCCGCGAGTC GCATCTGCTC AACGACGAGG 
     1141 ACCTGACGCA GGCAAAACCC ATTTATGGCG GTTGGCTGCT CCTGGCTCCA GATGGGACCG 
     1201 ACTTTGACAA CCCAGTGCAC CGGTCTCGGA AATGGCAGCG ACGGTTCTTC ATCCTTTACG 
     1261 AGCACGGCCT CTTGCGCTAC GCCCTGGATG AGATGCCCAC GACCCTTCCT CAGGGCACCA 
     1321 TCAACATGAA CCAGTGCACA GATGTGGTGG ATGGGGAGGG CCGCACGGGC CAGAAGTTCT 
     1381 CCCTGTGTAT TCTGACGCCT GAGAAGGAGC ATTTCATCCG GGCGGAGACC AAGGAGATCG 
     1441 TCAGTGGGTG GCTGGAGATG CTCATGGTCT ATCCCCGGAC CAACAAGCAG AATCAGAAGA 
     1501 AGAAACGGAA AGTGGAGCCC CCCACACCAC AGGAGCCTGG GCCTGCCAAG GTGGCTGTTA 
     1561 CCAGCAGCAG CAGCAGCAGC AGCAGCAGCA GCAGCATCCC CAGTGCTGAG AAAGTCCCCA 
     1621 CCACCAAGTC CACACTCTGG CAGGAAGAAA TGAGGACCAA GGACCAGCCA GATGGCAGCA 
     1681 GCCTGAGTCC AGCTCAGAGT CCCAGCCAGA GCCAGCCTCC TGCTGCCAGC TCCCTGCGGG 
     1741 AACCTGGGCT AGAGAGCAAA GAAGAGGAGA GCGCCATGAG TAGCGACCGC ATGGACTGTG 
     1801 GCCGCAAAGT CCGGGTGGAG AGCGGCTACT TCTCTCTGGA GAAGACCAAA CAGGACTTGA 
     1861 AGGCTGAAGA ACAGCAGCTG CCCCCGCCGC TCTCCCCTCC CAGCCCCAGC ACCCCCAACC 
     1921 ACAGGAGGTC CCAGGTGATT GAAAAGTTTG AGGCCTTGGA CATTGAGAAG GCAGAGCACA 
     1981 TGGAGACCAA TGCAGTGGGG CCCTCACCAT CCAGCGACAC ACGCCAGGGC CGCAGCGAGA 
     2041 AGAGGGCGTT CCCTAGGAAG CGGGACTTCA CCAATGAAGC CCCCCCAGCT CCTCTCCCAG 
     2101 ACGCCTCGGC TTCCCCCCTG TCTCCACACC GAAGAGCCAA GTCACTGGAC AGGAGGTCCA 
     2161 CGGAGCCCTC CGTGACGCCC GACCTGCTGA ATTTCAAGAA AGGCTGGCTG ACTAAGCAGT 
     2221 ATGAGGACGG CCAGTGGAAG AAACACTGGT TTGTCCTCGC CGATCAAAGC CTGAGATACT 
     2281 ACAGGGATTC AGTGGCTGAG GAGGCAGCCG ACTTGGATGG AGAAATTGAC TTGTCCGCAT 
     2341 GTTACGATGT CACAGAGTAT CCAGTTCAGA GAAACTATGG CTTCCAGATA CATACAAAGG 
     2401 AGGGCGAGTT TACCCTGTCG GCCATGACAT CTGGGATTCG GCGGAACTGG ATCCAGACCA 
     2461 TCATGAAGCA CGTGCACCCG ACCACTGCCC CGGATGTGAC CAGCTCGTTG CCAGAGGAAA 
     2521 AAAACAAGAG CAGCTGCTCT TTTGAGACCT GCCCGAGGCC TACTGAGAAG CAAGAGGCAG 
     2581 AGCTGGGGGA GCCGGACCCT GAGCAGAAGA GGAGCCGCGC ACGGGAGCGG AGGCGAGAGG 
     2641 GCCGCTCCAA GACCTTTGAC TGGGCTGAGT TCCGTCCCAT CCAGCAGGCC CTGGCTCAGG 
     2701 AGCGGGTGGG CGGCGTGGGG CCTGCTGACA CCCACGAGCC CCTGCGCCCT GAGGCGGAGC 
     2761 CTGGGGAGCT GGAGCGGGAG CGTGCACGGA GGCGGGAGGA GCGCCGCAAG CGCTTCGGGA 
     2821 TGCTCGACGC CACAGACGGG CCAGGCACTG AGGATGCAGC CCTGCGCATG GAGGTGGACC 
     2881 GGAGCCCAGG GCTGCCTATG AGCGACCTCA AAACGCATAA CGTCCACGTG GAGATTGAGC 
     2941 AGCGGTGGCA TCAGGTGGAG ACCACACCTC TCCGGGAAGA GAAGCAGGTG CCCATCGCCC 
     3001 CCGTCCACCT GTCTTCTGAA GATGGGGGTG ACCGGCTCTC CACACACGAG CTGACCTCTC 
     3061 TGCTCGAGAA GGAGCTGGAG CAGAGCCAGA AGGAGGCCTC AGACCTTCTG GAGCAGAACC 
     3121 GGCTCCTGCA GGACCAGCTG AGGGTGGCCC TGGGCCGGGA GCAGAGCGCC CGTGAGGGCT 
     3181 ACGTGCTGCA GGCCACGTGC GAGCGAGGGT TTGCAGCAAT GGAAGAAACG CACCAGAAGA 
     3241 AGATTGAAGA TCTCCAGAGG CAGCACCAGC GGGAGCTAGA GAAACTTCGA GAAGAGAAAG 
     3301 ACCGCCTCCT AGCCGAGGAG ACAGCGGCCA CCATCTCAGC CATCGAAGCC ATGAAGAACG 
     3361 CCCACCGGGA GGAAATGGAG CGGGAGCTGG AGAAGAGCCA GCGGTCCCAG ATCAGCAGCG 
     3421 TCAACTCGGA TGTTGAGGCC CTGCGGCGCC AGTACCTGGA GGAGCTGCAG TCGGTGCAGC 
     3481 GGGAACTGGA GGTCCTCTCG GAGCAGTACT CGCAGAAGTG CCTGGAGAAT GCCCATCTGG 
     3541 CCCAGGCGCT GGAGGCCGAG CGGCAGGCCC TGCGGCAGTG CCAGCGTGAG AACCAGGAGC 
     3601 TCAATGCCCA CAACCAGGAG CTGAACAACC GCCTGGCTGC AGAGATCACA CGGTTGCGGA 
     3661 CGCTGCTGAC TGGGGACGGC GGTGGGGAGG CCACTGGGTC ACCCCTTGCA CAGGGCAAGG 
     3721 ATGCCTATGA ACTAGAGGTC TTATTGCGGG TAAAGGAATC GGAAATACAG TACCTGAAAC 
     3781 AGGAGATTAG CTCCCTCAAG GATGAGCTGC AGACGGCACT GCGGGACAAG AAGTACGCAA 
     3841 GTGACAAGTA CAAAGACATC TACACAGAGC TCAGCATCGC GAAGGCTAAG GCTGACTGTG 
     3901 ACATCAGCAG GTTGAAGGAG CAGCTCAAGG CTGCAACGGA AGCACTGGGG GAGAAGTCCC 
     3961 CTGACAGTGC CACGGTGTCC GGATATGATA TAATGAAATC TAAAAGCAAC CCTGACTTCT 
     4021 TGAAGAAAGA CAGATCCTGT GTCACCCGGC AACTCAGAAA CATCAGGTCC AAGTCCGTAA 
     4081 TTGAGCAGGT CTCGTGGGAT ACCACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     4141 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     4201 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     4261 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     4321 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     4381 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     4441 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     4501 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     4561 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     4621 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     4681 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     4741 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     4801 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     4861 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     4921 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     4981 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     5041 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     5101 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     5161 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     5221 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     5281 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     5341 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     5401 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     5461 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     5521 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     5581 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     5641 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     5701 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     5761 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     5821 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     5881 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     5941 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     6001 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     6061 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     6121 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     6181 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     6241 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     6301 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     6361 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     6421 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     6481 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     6541 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     6601 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     6661 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     6721 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     6781 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     6841 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     6901 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     6961 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     7021 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     7081 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     7141 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     7201 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7261 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7321 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     7381 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     7441 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     7501 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     7561 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     7621 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     7681 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     7741 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     7801 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     7861 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     7921 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//