LOCUS       RC224282                9015 bp    DNA     circular  SYN 
DEFINITION  ROBO2 (Myc-DDK-tagged)-Human roundabout, axon guidance receptor, homolog 2 (Drosophila) (ROBO2), transcript variant 2
ACCESSION   NM_002942
VERSION     NM_002942.4
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5172..5263 
                     /label="Myc-DDK"  
     misc_feature    5293..5878 
                     /label="PolyA signal"  
     misc_feature    5350..5369 
                     /label="Restriction Enzyme"  
     misc_feature    6154..6826 
                     /label="ColE1"  
     misc_feature    7256..8050 
                     /label="Kan/Neo"  
     misc_feature    8085..8387 
                     /label="SV40 ori"  
     misc_feature    8473..8950 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5154..5160 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5154 
                     /label="ROBO2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGTCTGCTG ATGTTTACAC AACTACTGCT CTGTGGATTT TTATATGTTC 
     1081 GGGTTGATGG ATCGCGTCTT CGCCAGGAGG ACTTTCCCCC GCGGATTGTG GAGCATCCTT 
     1141 CCGATGTCAT CGTCTCTAAG GGCGAGCCCA CGACTCTGAA CTGCAAGGCG GAGGGCCGGC 
     1201 CAACGCCCAC CATTGAGTGG TACAAAGATG GGGAGCGAGT GGAGACTGAC AAGGACGATC 
     1261 CCCGGTCCCA CAGGATGCTT CTGCCCAGCG GATCCTTATT CTTCTTGCGC ATCGTGCACG 
     1321 GGCGCAGGAG TAAACCTGAT GAAGGAAGCT ACGTTTGTGT TGCGAGGAAC TATCTTGGTG 
     1381 AAGCAGTGAG TCGAAATGCG TCTCTGGAAG TGGCATTGTT ACGAGATGAC TTCCGACAAA 
     1441 ACCCCACAGA TGTTGTAGTG GCAGCTGGAG AGCCTGCAAT CCTGGAGTGC CAGCCTCCCC 
     1501 GGGGACACCC AGAACCCACC ATCTACTGGA AAAAAGACAA AGTTCGAATT GATGACAAGG 
     1561 AAGAAAGAAT AAGTATCCGT GGTGGAAAAC TGATGATCTC CAATACCAGG AAAAGTGATG 
     1621 CAGGGATGTA TACTTGTGTT GGTACCAATA TGGTGGGAGA AAGGGACAGT GACCCAGCAG 
     1681 AGCTGACTGT CTTTGAACGA CCCACATTTC TCAGGAGGCC AATTAACCAG GTGGTACTGG 
     1741 AGGAAGAAGC TGTAGAATTT CGTTGTCAAG TCCAAGGAGA TCCTCAACCA ACTGTGAGGT 
     1801 GGAAAAAGGA TGATGCAGAC TTGCCAAGAG GAAGGTATGA CATCAAAGAC GATTACACAC 
     1861 TAAGAATTAA AAAGACCATG AGTACAGATG AAGGCACCTA TATGTGTATT GCTGAGAATC 
     1921 GGGTTGGAAA AATGGAAGCC TCTGCTACAC TCACCGTCCG AGCTCCCCCA CAGTTTGTGG 
     1981 TTCGGCCAAG AGATCAGATT GTTGCTCAAG GTCGAACAGT GACATTTCCC TGTGAAACTA 
     2041 AAGGAAACCC ACAGCCAGCT GTTTTTTGGC AGAAAGAAGG CAGCCAGAAC CTACTTTTCC 
     2101 CAAACCAACC CCAGCAGCCC AACAGTAGAT GCTCAGTGTC ACCAACTGGA GACCTCACAA 
     2161 TCACCAACAT TCAACGTTCC GACGCGGGTT ACTACATCTG CCAGGCTTTA ACTGTGGCAG 
     2221 GAAGCATTTT AGCAAAAGCT CAACTGGAGG TTACTGATGT TTTGACAGAT AGACCTCCAC 
     2281 CTATAATTCT ACAAGGCCCA GCCAACCAAA CGCTGGCAGT GGATGGTACA GCGTTACTGA 
     2341 AATGTAAAGC CACTGGTGAT CCTCTTCCTG TAATTAGCTG GTTAAAGGAG GGATTTACTT 
     2401 TTCCGGGTAG AGATCCAAGA GCAACAATTC AAGAGCAAGG CACACTGCAG ATTAAGAATT 
     2461 TACGGATTTC TGATACTGGC ACTTATACTT GTGTGGCTAC AAGTTCAAGT GGAGAGACTT 
     2521 CCTGGAGTGC AGTGCTGGAT GTGACAGAGT CTGGAGCAAC AATCAGTAAA AACTATGATT 
     2581 TAAGTGACCT GCCAGGGCCA CCATCCAAAC CGCAGGTCAC TGATGTTACT AAGAACAGTG 
     2641 TCACCTTGTC CTGGCAGCCA GGTACCCCTG GAACCCTTCC AGCAAGTGCA TATATCATTG 
     2701 AGGCTTTCAG CCAATCAGTG AGCAACAGCT GGCAGACCGT GGCAAACCAT GTAAAGACCA 
     2761 CCCTCTATAC TGTAAGAGGA CTGCGGCCCA ATACAATCTA CTTATTCATG GTCAGAGCGA 
     2821 TCAACCCCCA AGGTCTCAGT GACCCAAGTC CCATGTCAGA TCCTGTGCGC ACACAAGATA 
     2881 TCAGCCCACC AGCACAAGGA GTGGACCACA GGCAAGTGCA GAAAGAGCTA GGAGATGTCC 
     2941 TTGTCCGTCT TCATAATCCA GTTGTGCTGA CTCCCACCAC GGTTCAGGTC ACATGGACGG 
     3001 TTGATCGCCA ACCCCAGTTT ATCCAAGGCT ACCGAGTGAT GTATCGTCAG ACTTCAGGTC 
     3061 TGCAGGCGAC ATCTTCGTGG CAGAATTTAG ATGCCAAAGT CCCGACTGAA CGAAGTGCTG 
     3121 TCTTAGTCAA CCTGAAAAAG GGGGTGACTT ATGAAATTAA AGTACGGCCA TATTTTAATG 
     3181 AGTTCCAAGG AATGGATAGT GAATCTAAAA CGGTTCGTAC TACTGAAGAA GCCCCAAGTG 
     3241 CCCCACCACA GTCTGTCACT GTACTGACAG TTGGAAGCTA CAATAGCACA AGTATTAGTG 
     3301 TTTCCTGGGA TCCTCCTCCT CCAGATCACC AGAATGGAAT TATCCAAGAA TACAAGATCT 
     3361 GGTGTCTAGG AAATGAAACG CGATTCCATA TCAACAAAAC TGTGGATGCA GCCATTCGGT 
     3421 CCGTAATAAT TGGTGGATTA TTCCCAGGTA TTCAATACCG GGTAGAGGTT GCAGCTAGTA 
     3481 CCAGTGCAGG GGTTGGAGTA AAGAGTGAGC CACAGCCAAT AATAATCGGG AGACGCAATG 
     3541 AAGTTGTCAT TACTGAAAAC AATAACAGCA TAACTGAGCA AATCACTGAT GTGGTGAAGC 
     3601 AACCAGCCTT TATAGCTGGT ATTGGTGGTG CCTGCTGGGT AATTCTGATG GGTTTTAGCA 
     3661 TATGGTTGTA TTGGCGAAGA AAGAAGAGGA AGGGACTCAG TAATTATGCT GTTACGTTTC 
     3721 AAAGAGGAGA TGGAGGACTA ATGAGCAATG GAAGCCGTCC AGGTCTTCTC AATGCTGGTG 
     3781 ATCCCAGCTA TCCATGGCTT GCTGATTCTT GGCCAGCCAC GAGCTTGCCA GTAAATAATA 
     3841 GCAACAGTGG CCCAAATGAG ATTGGAAATT TTGGCCGTGG AGATGTGCTG CCACCAGTTC 
     3901 CAGGCCAAGG GGATAAAACA GCAACGATGC TCTCAGATGG AGCCATTTAT AGTAGCATTG 
     3961 ACTTCACTAC CAAAACCAGT TACAACAGTT CCAGCCAAAT AACACAGGCT ACCCCATATG 
     4021 CCACGACACA GATCTTGCAT TCCAACAGCA TACATGAATT GGCTGTCGAT CTGCCTGATC 
     4081 CACAATGGAA AAGCTCAATT CAGCAAAAAA CAGATCTGAT GGGATTTGGT TATTCTCTAC 
     4141 CTGATCAGAA CAAAGGTAAC AATGGTGGGA AAGGTGGAAA AAAGAAGAAA AATAAAAACT 
     4201 CTTCTAAACC ACAGAAAAAC AATGGATCCA CTTGGGCCAA TGTCCCTCTA CCTCCCCCCC 
     4261 CAGTCCAGCC CCTTCCTGGC ACGGAGCTGG AACACTATGC AGTGGAACAA CAAGAAAATG 
     4321 GCTATGACAG TGATAGCTGG TGCCCACCAT TGCCAGTACA AACTTACTTA CACCAAGGTC 
     4381 TGGAAGATGA ACTGGAAGAA GATGATGATA GGGTCCCAAC ACCTCCTGTT CGAGGCGTGG 
     4441 CTTCTTCTCC TGCTATCTCC TTTGGACAGC AGTCCACTGC AACTCTTACT CCATCCCCAC 
     4501 GGGAAGAGAT GCAACCCATG CTGCAGGCTC ACCTGGATGA GTTGACAAGA GCCTATCAGT 
     4561 TTGATATAGC AAAACAAACA TGGCACATTC AAAGCAATAA TCAACCTCCA CAGCCTCCAG 
     4621 TTCCACCGTT AGGTTATGTG TCTGGAGCCT TGATTTCTGA TTTGGAAACG GATGTTGCAG 
     4681 ATGATGATGC CGACGACGAA GAGGAAGCTT TAGAAATCCC CAGGCCCCTG AGAGCACTGG 
     4741 ACCAGACTCC TGGATCCAGC ATGGACAATC TAGACAGCTC TGTGACAGGA AAAGCCTTTA 
     4801 CCTCCTCTCA AAGACCTCGA CCTACCAGCC CATTTTCTAC TGACAGTAAC ACCAGTGCAG 
     4861 CCCTGAGTCA AAGTCAGAGG CCTCGGCCCA CTAAAAAACA CAAGGGAGGG CGGATGGACC 
     4921 AACAACCAGC ATTGCCTCAT CGAAGGGAAG GAATGACAGA TGAGGAGGCC TTGGTGCCCT 
     4981 ATAGCAAGCC CAGTTTCCCA TCTCCAGGTG GCCACAGCTC ATCAGGAACA GCTTCTTCTA 
     5041 AGGGATCCAC TGGACCTAGG AAAACCGAGG TGTTGAGAGC AGGCCACCAG CGCAATGCCA 
     5101 GCGACCTTCT TGACATAGGA TATATGGGCT CCAACAGTCA AGGACAGTTT ACAGGTGAAT 
     5161 TAACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     5221 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     5281 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     5341 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     5401 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     5461 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     5521 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     5581 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     5641 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     5701 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     5761 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     5821 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     5881 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     5941 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     6001 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     6061 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     6121 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     6181 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     6241 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     6301 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     6361 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     6421 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     6481 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     6541 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     6601 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     6661 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     6721 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     6781 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     6841 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     6901 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     6961 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     7021 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     7081 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     7141 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     7201 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     7261 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     7321 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     7381 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     7441 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     7501 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     7561 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     7621 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     7681 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     7741 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     7801 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     7861 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     7921 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     7981 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     8041 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     8101 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     8161 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     8221 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     8281 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     8341 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     8401 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     8461 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     8521 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     8581 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     8641 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     8701 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     8761 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     8821 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     8881 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     8941 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     9001 TTTAACGCGA ATTTT
//