LOCUS       RC224276                8154 bp    DNA     circular  SYN 
DEFINITION  ACLY (Myc-DDK-tagged)-Human ATP citrate lyase (ACLY), transcript variant 2
ACCESSION   NM_198830
VERSION     NM_198830.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4311..4402 
                     /label="Myc-DDK"  
     misc_feature    4432..5017 
                     /label="PolyA signal"  
     misc_feature    4489..4508 
                     /label="Restriction Enzyme"  
     misc_feature    5293..5965 
                     /label="ColE1"  
     misc_feature    6395..7189 
                     /label="Kan/Neo"  
     misc_feature    7224..7526 
                     /label="SV40 ori"  
     misc_feature    7612..8089 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4293..4299 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4293 
                     /label="ACLY"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCGGCCAAG GCAATTTCAG AGCAGACGGG CAAAGAACTC CTTTACAAGT 
     1081 TCATCTGTAC CACCTCAGCC ATCCAGAATC GGTTCAAGTA TGCTCGGGTC ACTCCTGACA 
     1141 CAGACTGGGC CCGCTTGCTG CAGGACCACC CCTGGCTGCT CAGCCAGAAC TTGGTAGTCA 
     1201 AGCCAGACCA GCTGATCAAA CGTCGTGGAA AACTTGGTCT CGTTGGGGTC AACCTCACTC 
     1261 TGGATGGGGT CAAGTCCTGG CTGAAGCCAC GGCTGGGACA GGAAGCCACA GTTGGCAAGG 
     1321 CCACAGGCTT CCTCAAGAAC TTTCTGATCG AGCCCTTCGT CCCCCACAGT CAGGCTGAGG 
     1381 AGTTCTATGT CTGCATCTAT GCCACCCGAG AAGGGGACTA CGTCCTGTTC CACCACGAGG 
     1441 GGGGTGTGGA CGTGGGTGAT GTGGACGCCA AGGCCCAGAA GCTGCTTGTT GGCGTGGATG 
     1501 AGAAACTGAA TCCTGAGGAC ATCAAAAAAC ACCTGTTGGT CCACGCCCCT GAAGACAAGA 
     1561 AAGAAATTCT GGCCAGTTTT ATCTCCGGCC TCTTCAATTT CTACGAGGAC TTGTACTTCA 
     1621 CCTACCTCGA GATCAATCCC CTTGTAGTGA CCAAAGATGG AGTCTATGTC CTTGACTTGG 
     1681 CGGCCAAGGT GGACGCCACT GCCGACTACA TCTGCAAAGT GAAGTGGGGT GACATCGAGT 
     1741 TCCCTCCCCC CTTCGGGCGG GAGGCATATC CAGAGGAAGC CTACATTGCA GACCTCGATG 
     1801 CCAAAAGTGG GGCAAGCCTG AAGCTGACCT TGCTGAACCC CAAAGGGAGG ATCTGGACCA 
     1861 TGGTGGCCGG GGGTGGCGCC TCTGTCGTGT ACAGCGATAC CATCTGTGAT CTAGGGGGTG 
     1921 TCAACGAGCT GGCAAACTAT GGGGAGTACT CAGGCGCCCC CAGCGAGCAG CAGACCTATG 
     1981 ACTATGCCAA GACTATCCTC TCCCTCATGA CCCGAGAGAA GCACCCAGAT GGCAAGATCC 
     2041 TCATCATTGG AGGCAGCATC GCAAACTTCA CCAACGTGGC TGCCACGTTC AAGGGCATCG 
     2101 TGAGAGCAAT TCGAGATTAC CAGGGCCCCC TGAAGGAGCA CGAAGTCACA ATCTTTGTCC 
     2161 GAAGAGGTGG CCCCAACTAT CAGGAGGGCT TACGGGTGAT GGGAGAAGTC GGGAAGACCA 
     2221 CTGGGATCCC CATCCATGTC TTTGGCACAG AGACTCACAT GACGGCCATT GTGGGCATGG 
     2281 CCCTGGGCCA CCGGCCCATC CCCAACCAGC CACCCACAGC GGCCCACACT GCAAACTTCC 
     2341 TCCTCAACGC CAGCGGGAGC ACATCGACGC CAGCCCCCAG CAGGACAGCA TCTTTTTCTG 
     2401 AGTCCAGGGC CGATGAGGTG GCGCCTGCAA AGAAGGCCAA GCCTGCCATG CCACAAGGAA 
     2461 AGAGCACCAC CCTCTTCAGC CGCCACACCA AGGCCATTGT GTGGGGCATG CAGACCCGGG 
     2521 CCGTGCAAGG CATGCTGGAC TTTGACTATG TCTGCTCCCG AGACGAGCCC TCAGTGGCTG 
     2581 CCATGGTCTA CCCTTTCACT GGGGACCACA AGCAGAAGTT TTACTGGGGG CACAAAGAGA 
     2641 TCCTGATCCC TGTCTTCAAG AACATGGCTG ATGCCATGAG GAAGCATCCG GAGGTAGATG 
     2701 TGCTCATCAA CTTTGCCTCT CTCCGCTCTG CCTATGACAG CACCATGGAG ACCATGAACT 
     2761 ATGCCCAGAT CCGGACCATC GCCATCATAG CTGAAGGCAT CCCTGAGGCC CTCACGAGAA 
     2821 AGCTGATCAA GAAGGCGGAC CAGAAGGGAG TGACCATCAT CGGACCTGCC ACTGTTGGAG 
     2881 GCATCAAGCC TGGGTGCTTT AAGATTGGCA ACACAGGTGG GATGCTGGAC AACATCCTGG 
     2941 CCTCCAAACT GTACCGCCCA GGCAGCGTGG CCTATGTCTC ACGTTCCGGA GGCATGTCCA 
     3001 ACGAGCTCAA CAATATCATC TCTCGGACCA CGGATGGCGT CTATGAGGGC GTGGCCATTG 
     3061 GTGGGGACAG GTACCCGGGC TCCACATTCA TGGATCATGT GTTACGCTAT CAGGACACTC 
     3121 CAGGAGTCAA AATGATTGTG GTTCTTGGAG AGATTGGGGG CACTGAGGAA TATAAGATTT 
     3181 GCCGGGGCAT CAAGGAGGGC CGCCTCACTA AGCCCATCGT CTGCTGGTGC ATCGGGACGT 
     3241 GTGCCACCAT GTTCTCCTCT GAGGTCCAGT TTGGCCATGC TGGAGCTTGT GCCAACCAGG 
     3301 CTTCTGAAAC TGCAGTAGCC AAGAACCAGG CTTTGAAGGA AGCAGGAGTG TTTGTGCCCC 
     3361 GGAGCTTTGA TGAGCTTGGA GAGATCATCC AGTCTGTATA CGAAGATCTC GTGGCCAATG 
     3421 GAGTCATTGT ACCTGCCCAG GAGGTGCCGC CCCCAACCGT GCCCATGGAC TACTCCTGGG 
     3481 CCAGGGAGCT TGGTTTGATC CGCAAACCTG CCTCGTTCAT GACCAGCATC TGCGATGAGC 
     3541 GAGGACAGGA GCTCATCTAC GCGGGCATGC CCATCACTGA GGTCTTCAAG GAAGAGATGG 
     3601 GCATTGGCGG GGTCCTCGGC CTCCTCTGGT TCCAGAAAAG GTTGCCTAAG TACTCTTGCC 
     3661 AGTTCATTGA GATGTGTCTG ATGGTGACAG CTGATCACGG GCCAGCCGTC TCTGGAGCCC 
     3721 ACAACACCAT CATTTGTGCG CGAGCTGGGA AAGACCTGGT CTCCAGCCTC ACCTCGGGGC 
     3781 TGCTCACCAT CGGGGATCGG TTTGGGGGTG CCTTGGATGC AGCAGCCAAG ATGTTCAGTA 
     3841 AAGCCTTTGA CAGTGGCATT ATCCCCATGG AGTTTGTGAA CAAGATGAAG AAGGAAGGGA 
     3901 AGCTGATCAT GGGCATTGGT CACCGAGTGA AGTCGATAAA CAACCCAGAC ATGCGAGTGC 
     3961 AGATCCTCAA AGATTACGTC AGGCAGCACT TCCCTGCCAC TCCTCTGCTC GATTATGCAC 
     4021 TGGAAGTAGA GAAGATTACC ACCTCGAAGA AGCCAAATCT TATCCTGAAT GTAGATGGTC 
     4081 TCATCGGAGT CGCATTTGTA GACATGCTTA GAAACTGTGG GTCCTTTACT CGGGAGGAAG 
     4141 CTGATGAATA TATTGACATT GGAGCCCTCA ATGGCATCTT TGTGCTGGGA AGGAGTATGG 
     4201 GGTTCATTGG ACACTATCTT GATCAGAAGA GGCTGAAGCA GGGGCTGTAT CGTCATCCGT 
     4261 GGGATGATAT TTCATATGTT CTTCCGGAAC ACATGAGCAT GACGCGTACG CGGCCGCTCG 
     4321 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     4381 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     4441 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     4501 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     4561 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     4621 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     4681 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     4741 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     4801 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     4861 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     4921 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     4981 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     5041 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     5101 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     5161 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     5221 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     5281 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     5341 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     5401 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     5461 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     5521 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     5581 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     5641 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     5701 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     5761 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     5821 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     5881 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     5941 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     6001 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     6061 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     6121 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     6181 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     6241 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     6301 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     6361 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     6421 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     6481 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     6541 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     6601 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     6661 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     6721 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     6781 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     6841 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     6901 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     6961 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     7021 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     7081 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     7141 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     7201 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     7261 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     7321 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     7381 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7441 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     7501 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     7561 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     7621 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     7681 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     7741 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     7801 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     7861 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     7921 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     7981 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     8041 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     8101 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//