LOCUS       RC224257                7674 bp    DNA     circular  SYN 
DEFINITION  UNC5C (Myc-DDK-tagged)-Human unc-5 homolog C (C. elegans) (UNC5C)
ACCESSION   NM_003728
VERSION     NM_003728.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3831..3922 
                     /label="Myc-DDK"  
     misc_feature    3952..4537 
                     /label="PolyA signal"  
     misc_feature    4009..4028 
                     /label="Restriction Enzyme"  
     misc_feature    4813..5485 
                     /label="ColE1"  
     misc_feature    5915..6709 
                     /label="Kan/Neo"  
     misc_feature    6744..7046 
                     /label="SV40 ori"  
     misc_feature    7132..7609 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3813..3819 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3813 
                     /label="UNC5C"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGGAAAGGT CTGCGGGCGA CAGCGGCCCG CTGCGGACTG GGACTGGGAT 
     1081 ACTTGCTGCA AATGCTCGTG CTACCTGCCC TGGCCCTGCT CAGCGCCAGC GGCACTGGCT 
     1141 CCGCCGCCCA AGATGATGAC TTTTTTCATG AACTCCCAGA AACTTTTCCT TCTGATCCAC 
     1201 CTGAGCCTCT GCCACATTTC CTTATTGAGC CTGAAGAAGC TTATATTGTG AAGAATAAGC 
     1261 CTGTGAACCT GTACTGTAAA GCAAGCCCTG CCACCCAGAT CTATTTCAAG TGTAATAGTG 
     1321 AATGGGTTCA TCAGAAGGAC CACATAGTAG ATGAAAGAGT AGATGAAACT TCCGGTCTCA 
     1381 TTGTCCGGGA AGTGAGCATT GAGATTTCGC GCCAGCAAGT GGAAGAACTC TTTGGACCTG 
     1441 AAGATTACTG GTGCCAGTGT GTGGCCTGGA GCTCCGCGGG TACCACAAAG AGCCGGAAGG 
     1501 CGTATGTGCG CATTGCATAT CTACGGAAGA CATTTGAGCA GGAACCCCTA GGAAAGGAAG 
     1561 TGTCTTTGGA ACAGGAAGTC TTACTCCAGT GTCGACCACC TGAAGGGATC CCAGTGGCTG 
     1621 AGGTGGAATG GTTGAAAAAT GAAGACATAA TTGATCCCGT TGAAGATCGG AATTTTTATA 
     1681 TTACTATTGA TCACAACCTC ATCATAAAGC AGGCCCGACT CTCTGATACT GCAAATTACA 
     1741 CCTGTGTTGC CAAAAACATT GTTGCCAAGA GGAAAAGTAC AACTGCCACT GTCATAGTCT 
     1801 ATGTCAACGG TGGCTGGTCC ACCTGGACGG AGTGGTCTGT GTGTAACAGC CGCTGTGGAC 
     1861 GAGGGTATCA GAAACGTACA AGGACTTGTA CCAACCCGGC ACCACTCAAT GGGGGTGCCT 
     1921 TCTGTGAAGG GCAGAGTGTG CAGAAAATAG CCTGTACTAC GTTATGCCCA GTGGATGGCA 
     1981 GGTGGACGCC ATGGAGCAAG TGGTCTACTT GTGGAACTGA GTGCACCCAC TGGCGCAGGA 
     2041 GGGAGTGCAC GGCGCCAGCC CCCAAGAATG GAGGCAAGGA CTGCGACGGC CTCGTCTTGC 
     2101 AATCCAAGAA CTGCACTGAT GGGCTTTGCA TGCAGACTGC TCCTGATTCA GATGATGTTG 
     2161 CTCTCTATGT TGGGATTGTG ATAGCAGTGA TCGTTTGCCT GGCGATCTCT GTAGTTGTGG 
     2221 CCTTGTTTGT GTATCGGAAG AATCATCGTG ACTTTGAGTC AGATATTATT GACTCTTCGG 
     2281 CACTCAATGG GGGCTTTCAG CCTGTGAACA TCAAGGCAGC AAGACAAGAT CTGCTGGCTG 
     2341 TACCCCCAGA CCTCACGTCA GCTGCAGCCA TGTACAGAGG ACCTGTCTAT GCCCTGCATG 
     2401 ACGTCTCAGA CAAAATCCCA ATGACCAACT CTCCAATTCT GGATCCACTG CCCAACCTGA 
     2461 AAATCAAAGT GTACAACACC TCAGGTGCTG TCACCCCCCA AGATGACCTC TCTGAGTTTA 
     2521 CGTCCAAGCT GTCCCCTCAG ATGACCCAGT CGTTGTTGGA GAATGAAGCC CTCAGCCTGA 
     2581 AGAACCAGAG TCTAGCAAGG CAGACTGATC CATCCTGTAC CGCATTTGGC AGCTTCAACT 
     2641 CGCTGGGAGG TCACCTTATT GTTCCCAATT CAGGAGTCAG CTTGCTGATT CCCGCTGGGG 
     2701 CCATTCCCCA AGGGAGAGTC TACGAAATGT ATGTGACTGT ACACAGGAAA GAAACTATGA 
     2761 GGCCACCCAT GGATGACTCT CAGACACTTT TGACCCCTGT GGTGAGCTGT GGGCCCCCAG 
     2821 GAGCTCTGCT CACCCGCCCC GTCGTCCTCA CTATGCATCA CTGCGCAGAC CCCAATACCG 
     2881 AGGACTGGAA AATACTGCTC AAGAACCAGG CAGCACAGGG ACAGTGGGAG GATGTGGTGG 
     2941 TGGTCGGGGA GGAAAACTTC ACCACCCCCT GCTACATTAA GCTGGATGCA GAGGCCTGCC 
     3001 ACATCCTCAC AGAGAACCTC AGCACCTACG CCCTGGTAGG ACATTCCACC ACCAAAGCGG 
     3061 CTGCAAAGCG CCTCAAGCTG GCCATCTTTG GGCCCCTGTG CTGCTCCTCG CTGGAGTACA 
     3121 GCATCCGAGT CTACTGTCTG GATGACACCC AGGATGCCCT GAAGGAAATT TTACATCTTG 
     3181 AGAGACAGAC GGGAGGACAG CTCCTAGAAG AACCTAAGGC TCTTCATTTT AAAGGCAGCA 
     3241 CCCACAACCT GCGCCTGTCA ATTCACGATA TCGCCCATTC CCTCTGGAAG AGCAAATTGC 
     3301 TGGCTAAATA TCAGGAAATT CCATTTTACC ATGTTTGGAG TGGATCTCAA AGAAACCTGC 
     3361 ACTGCACCTT CACTCTGGAA AGATTTAGCC TGAACACAGT GGAGCTGGTT TGCAAACTCT 
     3421 GTGTGCGGCA GGTGGAAGGA GAAGGGCAGA TCTTCCAGCT CAACTGCACC GTGTCAGAGG 
     3481 AACCTACTGG CATCGATTTG CCGCTGCTGG ATCCTGCGAA CACCATCACC ACGGTCACGG 
     3541 GGCCCAGTGC TTTCAGCATC CCTCTCCCTA TCCGGCAGAA GCTCTGTAGC AGCCTGGATG 
     3601 CCCCCCAGAC GAGAGGCCAT GACTGGAGGA TGCTGGCCCA TAAGCTGAAC CTGGACAGGT 
     3661 ACTTGAATTA CTTTGCCACC AAATCCAGCC CAACTGGCGT AATCCTGGAT CTTTGGGAAG 
     3721 CACAGAACTT CCCAGATGGA AACCTGAGCA TGCTGGCAGC TGTCTTGGAA GAAATGGGAA 
     3781 GACATGAAAC GGTGGTGTCC TTAGCAGCAG AAGGGCAGTA TACGCGTACG CGGCCGCTCG 
     3841 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     3901 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     3961 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     4021 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     4081 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     4141 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     4201 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     4261 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     4321 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     4381 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     4441 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     4501 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     4561 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     4621 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     4681 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     4741 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     4801 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     4861 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     4921 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     4981 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     5041 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     5101 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     5161 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     5221 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     5281 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     5341 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     5401 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     5461 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     5521 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     5581 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     5641 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     5701 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     5761 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     5821 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     5881 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     5941 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     6001 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     6061 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     6121 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     6181 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     6241 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     6301 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     6361 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     6421 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     6481 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     6541 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     6601 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     6661 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     6721 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     6781 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     6841 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     6901 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     6961 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     7021 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     7081 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     7141 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     7201 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     7261 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     7321 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     7381 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     7441 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     7501 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     7561 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     7621 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//