LOCUS       RC224247                7527 bp    DNA     circular  SYN 
DEFINITION  PIWIL3 (Myc-DDK-tagged)-Human piwi-like 3 (Drosophila) (PIWIL3)
ACCESSION   NM_001008496
VERSION     NM_001008496.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3684..3775 
                     /label="Myc-DDK"  
     misc_feature    3805..4390 
                     /label="PolyA signal"  
     misc_feature    3862..3881 
                     /label="Restriction Enzyme"  
     misc_feature    4666..5338 
                     /label="ColE1"  
     misc_feature    5768..6562 
                     /label="Kan/Neo"  
     misc_feature    6597..6899 
                     /label="SV40 ori"  
     misc_feature    6985..7462 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3666..3672 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3666 
                     /label="PIWIL3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCTGGTAGG GCAAGGACTC GCGCCCGAGG CAGAGCCCGC CGCAGGGAGA 
     1081 GCTACCAACA AGAGGCACCT GGGGGACCCA GAGCACCTGG ATCAGCTACA ACCCAGGAGC 
     1141 CCCCTCAGTT GCAGTCGACA CCCCGGCCGC TGCAGGAGGA AGTCCCAGTG GTTAGACCTC 
     1201 TGCAGCCAAG AGCAGCAAGA GGAGGAGCAG GAGGAGGAGC ACAGTCTCAA GGGGTGAAGG 
     1261 AACCTGGACC TGAGGCTGGG TTGCATACAG CGCCCTTGCA GGAGAGAAGG ATTGGTGGAG 
     1321 TTTTTCAAGA CCTGGTGGTG AACACCAGGC AAGATATGAA GCATGTTAAA GACTCAAAAA 
     1381 CAGGTTCAGA GGGTACAGTG GTACAGCTAC TCGCCAACCA CTTCCGAGTG ATATCTCGTC 
     1441 CTCAGTGGGT TGCATATAAA TACAACGTTG ACTACAAACC AGACATAGAA GATGGAAATC 
     1501 TCCGTACAAT TTTACTTGAT CAACATAGAA GGAAATTTGG AGAGCGCCAT ATATTTGATG 
     1561 GAAACTCTTT ATTATTATCT CGGCCACTAA AAGAGCGGAG AGTGGAATGG TTGAGCACAA 
     1621 CCAAAGACAA AAACATCGTG AAGATTACAG TTGAGTTTTC CAAAGAACTC ACGCCCACGT 
     1681 CGCCAGATTG CCTACGCTAT TACAACATTC TCTTTAGAAG AACTTTCAAG CTGCTGGATT 
     1741 TTGAACAAGT TGGTCGCAAC TATTATACCA AAAAGAAGGC CATTCAGTTA TACCGTCATG 
     1801 GTACCAGTTT GGAAATCTGG CTTGGTTATG TTACTTCTGT TCTTCAATAC GAAAACAGCA 
     1861 TTACCCTCTG TGCCGATGTG AGCCACAAAC TGCTCCGAAT AGAAACTGCT TATGATTTCA 
     1921 TAAAGAGAAC ATCTGCCCAG GCCCAGACAG GAAACATCCG AGAGGAAGTA ACTAATAAAT 
     1981 TAATTGGATC AATTGTTCTG ACAAAATACA ACAACAAAAC CTACAGAGTA GATGATATTG 
     2041 ATTGGAAGCA GAATCCTGAA GACACATTTA ACAAATCAGA TGGCAGCAAA ATCACCTATA 
     2101 TAGACTACTA CAGGCAGCAA CATAAAGAAA TTGTCACAGT GAAGAAACAG CCACTTTTGG 
     2161 TCAGCCAGGG CAGATGGAAA AAGGGCCTAA CGGGTACACA ACGTGAACCT ATCCTGCTGA 
     2221 TTCCTCAGCT GTGCCACATG ACAGGTCTAA CAGATGAAAT ATGTAAAGAT TATAGCATTG 
     2281 TGAAAGAATT GGCTAAACAT ACAAGATTGA GTCCAAGAAG AAGGCATCAT ACATTAAAAG 
     2341 AATTCATCAA TACTCTACAA GATAATAAAA AAGTACGAGA GTTACTTCAA CTCTGGGATT 
     2401 TGAAATTTGA TACCAATTTT TTGTCCGTCC CGGGAAGAGT TTTGAAAAAC GCAAACATCG 
     2461 TGCAAGGCAG AAGAATGGTT AAAGCCAATT CACAAGGAGA CTGGTCAAGA GAAATAAGAG 
     2521 AATTACCCTT ACTTAATGCA ATGCCACTAC ATAGTTGGCT CATACTCTAT AGCAGGAGCA 
     2581 GTCACAGAGA AGCCATGTCC TTAAAGGGTC ATCTACAGAG TGTCACAGCC CCCATGGGCA 
     2641 TAACTATGAA ACCAGCAGAA ATGATTGAAG TAGATGGTGA TGCTAACTCC TATATAGACA 
     2701 CATTACGGAA ATATACTAGA CCAACACTGC AGATGGGAAT GTCATGTTTG CTGGTTTTCA 
     2761 AGGTGATTTG TATCCTGCCC AATGATGACA AACGTAGATA TGACAGCATA AAAAGATACC 
     2821 TATGTACCAA ATGCCCAATT CCAAGCCAGT GTGTGGTGAA AAAGACCTTA GAAAAAGTCC 
     2881 AGGCAAGGAC CATCGTCACC AAGATTGCCC AGCAGATGAA TTGCAAGATG GGAGGAGCCC 
     2941 TCTGGAAGGT GGAGACAGAC GTACAAAGAA CAATGTTCGT TGGCATTGAT TGTTTCCACG 
     3001 ATATCGTAAA TCGACAGAAA TCAATAGCAG GATTTGTTGC AAGTACCAAT GCTGAATTAA 
     3061 CAAAGTGGTA CTCTCAATGT GTCATCCAGA AAACAGGAGA AGAGCTTGTG AAAGAGCTGG 
     3121 AGATCTGCTT GAAAGCTGCC CTGGATGTCT GGTGTAAAAA CGAATCATCG ATGCCACATT 
     3181 CTGTTATTGT GTATCGGGAT GGAGTGGGAG ATGGTCAGCT TCAAGCATTG CTTGACCATG 
     3241 AAGCGAAAAA GATGTCGACC TACTTAAAAA CCATCTCTCC TAACAATTTC ACTCTAGCTT 
     3301 TCATTGTGGT GAAGAAACGA ATAAACACTA GATTTTTTCT TAAACATGGA AGCAATTTTC 
     3361 AAAATCCACC TCCAGGAACA GTTATTGATG TAGAGTTGAC TAGGAATGAA TGGTATGACT 
     3421 TTTTTATTGT GAGTCAGTCT GTGCAAGATG GGACTGTTAC CCCCACTCAT TATAACGTCA 
     3481 TCTATGACAC GATTGGCTTG AGCCCAGATA CAGTACAGCG TTTAACATAT TGTCTATGCC 
     3541 ACATGTATTA TAATTTGCCA GGCATCATCC GAGTTCCAGC GCCTTGCCAC TATGCCCACA 
     3601 AGCTGGCTTA CCTCGTGGGG CAGTCCATTC ACCAGGAACC GAATCGTTCC TTGTCAACTC 
     3661 GTCTCTTTTA CCTTACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     3721 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     3781 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     3841 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     3901 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     3961 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     4021 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     4081 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     4141 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     4201 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     4261 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     4321 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     4381 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     4441 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     4501 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     4561 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     4621 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     4681 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     4741 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     4801 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     4861 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     4921 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     4981 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     5041 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     5101 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     5161 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     5221 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     5281 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     5341 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     5401 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     5461 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     5521 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     5581 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     5641 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     5701 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     5761 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     5821 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     5881 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     5941 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     6001 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     6061 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     6121 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     6181 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     6241 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     6301 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     6361 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     6421 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     6481 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     6541 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     6601 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     6661 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     6721 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     6781 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     6841 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     6901 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     6961 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     7021 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     7081 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     7141 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     7201 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     7261 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     7321 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     7381 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     7441 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     7501 ATTTAACAAA AATTTAACGC GAATTTT
//