LOCUS       RC224212                8745 bp    DNA     circular  SYN 
DEFINITION  ASTN2 (Myc-DDK-tagged)-Human astrotactin 2 (ASTN2), transcript variant 1
ACCESSION   NM_014010
VERSION     NM_014010.4
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4902..4993 
                     /label="Myc-DDK"  
     misc_feature    5023..5608 
                     /label="PolyA signal"  
     misc_feature    5080..5099 
                     /label="Restriction Enzyme"  
     misc_feature    5884..6556 
                     /label="ColE1"  
     misc_feature    6986..7780 
                     /label="Kan/Neo"  
     misc_feature    7815..8117 
                     /label="SV40 ori"  
     misc_feature    8203..8680 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4884..4890 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4884 
                     /label="ASTN2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCGCCGCC GGCGCCCGGC TCAGCCCCGG CCCCGGCTCG GGGCTCCGGG 
     1081 GGCGGCCGAG GCTCTGCTTC CACCCGGGGC CGCCGCCACT GCTGCCGCTG CTGCTGCTGT 
     1141 TCCTGCTCCT GCTGCCGCCG CCGCCGCTGC TGGCCGGCGC CACCGCCGCT GCCTCGCGGG 
     1201 AGCCCGACAG CCCGTGCCGG CTGAAGACCG TCACGGTGTC CACACTGCCC GCCCTGCGGG 
     1261 AGAGCGACAT CGGCTGGAGC GGCGCCCGCG CCGGGGCCGG GGCTGGGACC GGGGCCGGAG 
     1321 CCGCCGCCGC CGCCGCGTCC CCGGGCTCTC CTGGCTCTGC CGGCACCGCC GCCGAGTCGC 
     1381 GCCTCCTGCT CTTTGTGCGT AACGAGCTGC CGGGGCGCAT CGCGGTGCAG GACGACCTGG 
     1441 ACAACACCGA GCTGCCCTTC TTCACCCTGG AGATGTCTGG CACAGCGGCG GACATCTCGC 
     1501 TGGTGCACTG GAGACAGCAG TGGCTGGAGA ATGGCACCTT GTACTTCCAC GTCTCCATGA 
     1561 GCAGCTCCGG GCAGCTGGCC CAAGCCACCG CCCCCACTCT CCAGGAGCCC TCGGAGATTG 
     1621 TTGAGGAGCA GATGCACATC CTCCACATTT CTGTGATGGG TGGCCTCATC GCGCTGCTGC 
     1681 TGCTGCTGCT GGTGTTCACC GTGGCGCTGT ACGCCCAGCG ACGTTGGCAG AAGCGTCGCC 
     1741 GCATCCCCCA GAAGAGCGCA AGCACAGAAG CCACTCATGA GATCCACTAC ATCCCATCTG 
     1801 TGCTGCTGGG TCCCCAGGCG CGGGAGAGCT TCCGTTCATC CCGGCTGCAA ACCCACAATT 
     1861 CCGTCATTGG CGTGCCCATC CGGGAGACTC CCATCCTGGA TGACTATGAC TGTGAGGAGG 
     1921 ATGAGGAGCC ACCTAGGCGG GCCAACCATG TCTCCCGCGA GGACGAGTTT GGCAGCCAGG 
     1981 TGACCCACAC TCTGGACAGT CTGGGACATC CAGGGGAAGA GAAGGTGGAC TTTGAGAAGA 
     2041 AAGGAGGAAT CAGCTTTGGG AGAGCCAAGG GGACGTCGGG CTCAGAGGCA GACGATGAAA 
     2101 CTCAGCTGAC ATTCTACACG GAGCAGTACC GCAGTCGCCG CCGCAGCAAA GGTTTGCTGA 
     2161 AAAGCCCAGT GAACAAGACA GCCCTGACAC TGATTGCTGT GAGTTCCTGC ATCCTGGCCA 
     2221 TGGTGTGTGG CAGCCAGATG TCTTGTCCAC TCACTGTGAA GGTGACTCTG CATGTGCCCG 
     2281 AGCACTTCAT AGCAGATGGA AGCAGCTTCG TGGTGAGTGA AGGGAGCTAC CTGGACATCT 
     2341 CCGACTGGTT AAACCCGGCC AAGCTTTCCC TGTATTACCA GATCAATGCC ACCTCCCCAT 
     2401 GGGTGAGGGA CCTCTGTGGA CAAAGGACGA CAGATGCCTG TGAGCAGCTC TGCGACCCAG 
     2461 AAACCGGAGA GTGCAGCTGT CATGAAGGCT ATGCCCCTGA CCCTGTTCAC AGACACCTGT 
     2521 GTGTGCGCAG TGACTGGGGA CAGAGTGAAG GACCTTGGCC CTACACGACA CTTGAGAGGG 
     2581 GCTATGATCT GGTGACAGGG GAGCAAGCCC CTGAAAAGAT TCTCAGGTCT ACTTTCAGCT 
     2641 TGGGCCAAGG CCTCTGGCTT CCTGTCAGCA AAAGCTTTGT GGTTCCGCCT GTGGAGCTGT 
     2701 CCATCAACCC CCTGGCCAGC TGCAAGACCG ATGTGCTCGT CACGGAAGAC CCTGCAGATG 
     2761 TCAGGGAAGA AGCGATGCTG TCCACATACT TTGAAACCAT CAATGACCTG CTGTCTTCCT 
     2821 TCGGGCCAGT TCGTGACTGC TCTCGGAACA ATGGGGGCTG CACTCGCAAC TTCAAGTGTG 
     2881 TGTCTGACCG GCAGGTGGAT TCCTCGGGAT GTGTGTGCCC TGAGGAGCTG AAACCCATGA 
     2941 AGGATGGCTC TGGCTGCTAC GACCACTCCA AAGGCATTGA CTGCTCTGAT GGCTTTAATG 
     3001 GCGGCTGTGA GCAGCTGTGC CTGCAGCAGA CGCTGCCCCT GCCCTACGAT GCCACTTCGA 
     3061 GCACCATCTT CATGTTCTGC GGTTGCGTGG AGGAGTACAA ACTGGCTCCT GATGGAAAAT 
     3121 CCTGCTTAAT GCTCTCAGAT GTCTGCGAGG GCCCCAAGTG CCTCAAACCT GACTCCAAAT 
     3181 TCAATGATAC CCTCTTTGGA GAGATGCTAC ATGGTTACAA CAACCGGACC CAGCATGTGA 
     3241 ACCAAGGCCA AGTCTTCCAG ATGACCTTTA GGGAGAACAA CTTCATCAAG GACTTTCCCC 
     3301 AGCTGGCCGA TGGGCTGTTG GTGATCCCGC TGCCGGTGGA GGAGCAGTGC CGGGGGGTCC 
     3361 TCTCCGAGCC CCTTCCAGAC CTCCAACTGC TCACTGGAGA TATCAGGTAT GATGAGGCCA 
     3421 TGGGTTACCC CATGGTGCAG CAGTGGCGGG TCCGGAGCAA CCTCTACCGT GTGAAGCTCA 
     3481 GCACCATCAC CCTCGCAGCA GGCTTCACTA ATGTTCTCAA GATCCTGACC AAGGAGAGCA 
     3541 GTCGGGAGGA GCTGCTGTCC TTCATCCAGC ACTATGGCTC CCACTACATC GCAGAGGCCC 
     3601 TCTATGGCTC AGAGCTCACC TGCATCATCC ACTTTCCCAG CAAGAAGGTC CAGCAGCAGC 
     3661 TGTGGCTCCA GTATCAGAAA GAGACCACAG AGCTGGGCAG CAAGAAGGAG CTCAAGTCCA 
     3721 TGCCCTTCAT CACCTACCTC TCAGGTTTGC TGACAGCCCA GATGCTGTCA GATGACCAGC 
     3781 TCATTTCAGG TGTGGAGATT CGCTGTGAGG AGAAGGGGCG CTGTCCATCT ACCTGTCACC 
     3841 TTTGCCGCCG GCCAGGCAAG GAGCAGCTGA GCCCCACACC AGTGCTGCTG GAAATCAACC 
     3901 GTGTGGTGCC ACTTTATACC CTCATCCAAG ACAATGGCAC AAAGGAGGCC TTCAAGAGTG 
     3961 CACTGATGAG TTCCTACTGG TGCTCAGGGA AAGGGGATGT GATCGATGAC TGGTGCAGGT 
     4021 GTGACCTCAG CGCCTTTGAT GCCAATGGGC TCCCCAACTG CAGCCCCCTT CTGCAGCCGG 
     4081 TGCTGCGGCT GTCCCCAACA GTGGAGCCCT CCAGTACTGT GGTCTCCTTG GAGTGGGTGG 
     4141 ATGTTCAGCC AGCTATTGGG ACCAAGGTCT CCGACTATAT TCTGCAGCAT AAGAAAGTGG 
     4201 ATGAATACAC AGACACTGAC CTGTACACAG GAGAATTCCT GAGTTTTGCT GATGACTTAC 
     4261 TCTCTGGCCT GGGCACATCT TGTGTAGCAG CTGGTCGAAG CCATGGAGAG GTCCCTGAAG 
     4321 TCAGTATCTA CTCAGTCATC TTCAAGTGTC TGGAGCCCGA CGGTCTCTAC AAGTTCACTC 
     4381 TGTATGCTGT GGATACACGA GGGAGGCACT CAGAGCTAAG CACGGTGACC CTGAGGACGG 
     4441 CCTGTCCACT GGTAGATGAC AACAAGGCAG AAGAAATAGC TGACAAGATC TACAATCTGT 
     4501 ACAATGGGTA CACAAGTGGA AAGGAGCAGC AGATGGCCTA CAACACACTG ATGGAGGTCT 
     4561 CAGCCTCGAT GCTGTTCCGA GTCCAGCACC ACTACAACTC TCACTATGAA AAGTTTGGCG 
     4621 ACTTCGTCTG GAGAAGTGAG GATGAGCTGG GGCCCAGGAA GGCCCACCTG ATTCTACGGC 
     4681 GACTGGAGAG GGTGAGTAGC CACTGCTCCA GCCTCCTGCG GAGTGCCTAC ATCCAGAGCC 
     4741 GCGTGGAAAC AGTGCCCTAT CTTTTCTGCC GCAGCGAGGA GGTCCGGCCT GCAGGCATGG 
     4801 TGTGGTATAG CATCCTCAAG GACACCAAAA TCACGTGTGA GGAGAAGATG GTGTCAATGG 
     4861 CCCGAAACAC GTACGGGGAG TCCAAGGGCC GGACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     4921 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     4981 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     5041 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     5101 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     5161 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     5221 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     5281 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     5341 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     5401 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     5461 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     5521 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     5581 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     5641 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     5701 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     5761 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     5821 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     5881 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     5941 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     6001 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     6061 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     6121 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     6181 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     6241 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     6301 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     6361 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     6421 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     6481 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     6541 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     6601 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     6661 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     6721 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     6781 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     6841 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     6901 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     6961 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     7021 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     7081 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     7141 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     7201 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     7261 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     7321 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     7381 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     7441 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     7501 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     7561 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     7621 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     7681 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     7741 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     7801 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     7861 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     7921 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     7981 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     8041 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     8101 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     8161 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     8221 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     8281 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     8341 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     8401 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     8461 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     8521 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     8581 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     8641 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     8701 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//