LOCUS       RC224123                7503 bp    DNA     circular  SYN 
DEFINITION  GPR133 (Myc-DDK-tagged)-Human G protein-coupled receptor 133 (GPR133)
ACCESSION   NM_198827
VERSION     NM_198827.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3660..3751 
                     /label="Myc-DDK"  
     misc_feature    3781..4366 
                     /label="PolyA signal"  
     misc_feature    3838..3857 
                     /label="Restriction Enzyme"  
     misc_feature    4642..5314 
                     /label="ColE1"  
     misc_feature    5744..6538 
                     /label="Kan/Neo"  
     misc_feature    6573..6875 
                     /label="SV40 ori"  
     misc_feature    6961..7438 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3642..3648 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3642 
                     /label="ADGRD1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAAAAGCTG CTGCGGCTGT GCTGCTGGTA CTCCTGGCTG CTGCTATTTT 
     1081 ATTACAACTT TCAGGTGCGT GGCGTCTACT CCAGATCGCA GGACCATCCA GGATTTCAGG 
     1141 TGTTGGCGTC TGCTTCCCAT TACTGGCCAC TGGAGAATGT GGATGGGATC CATGAACTTC 
     1201 AGGATACAAC TGGAGATATT GTGGAAGGGA AGGTCAACAA AGGCATTTAC CTGAAAGAGG 
     1261 AAAAGGGAGT CACGCTTCTC TATTACGGCA GGTACAACAG CTCCTGCATC AGCAAGCCAG 
     1321 AGCAGTGTGG CCCTGAAGGG GTCACGTTTT CTTTTTTCTG GAAGACACAA GGAGAACAGT 
     1381 CTAGACCAAT CCCTTCTGCG TATGGGGGAC AGGTCATCTC CAATGGGTTC AAAGTCTGCT 
     1441 CCAGCGGTGG CAGAGGCTCT GTGGAGCTGT ACACGCGGGA CAATTCCATG ACATGGGAGG 
     1501 CCTCCTTCAG CCCCCCAGGC CCCTATTGGA CTCATGTCCT ATTTACATGG AAATCCAAGG 
     1561 AGGGCCTGAA AGTCTACGTC AACGGGACCC TGAGCACCTC TGATCCGAGT GGAAAAGTGT 
     1621 CTCGTGACTA TGGAGAGTCC AACGTCAACC TCGTGATAGG GTCTGAGCAG GACCAGGCCA 
     1681 AGTGTTATGA GAACGGTGCT TTCGATGAGT TCATCATCTG GGAGCGGGCT CTGACTCCGG 
     1741 ATGAGATCGC CATGTACTTC ACTGCTGCCA TTGGAAAGCA TGCTTTATTG TCTTCAACGC 
     1801 TGCCAAGCCT CTTCATGACA TCCACAGCAA GCCCCGTGAT GCCCACAGAT GCCTACCATC 
     1861 CCATCATAAC CAACCTGACA GAAGAGAGAA AAACCTTCCA AAGTCCCGGA GTGATACTGA 
     1921 GTTACCTCCA AAATGTATCC CTCAGCTTAC CCAGTAAGTC CCTCTCGGAG CAGACAGCCT 
     1981 TGAATCTCAC CAAGACCTTC TTAAAAGCCG TGGGAGAGAT CCTTCTACTG CCTGGTTGGA 
     2041 TTGCTCTGTC AGAGGACAGC GCCGTGGTAC TGAGTCTCAT CGACACTATT GACACCGTCA 
     2101 TGGGCCATGT ATCCTCCAAC CTGCACGGCA GCACGCCCCA GGTCACCGTG GAGGGCTCCT 
     2161 CTGCCATGGC AGAGTTTTCC GTGGCCAAAA TCCTGCCCAA GACCGTGAAT TCCTCCCATT 
     2221 ACCGCTTCCC GGCCCACGGG CAGAGCTTCA TCCAGATCCC CCACGAGGCC TTCCACAGGC 
     2281 ACGCCTGGAG CACCGTCGTG GGTCTGCTGT ACCACAGCAT GCACTACTAC CTGAACAACA 
     2341 TCTGGCCCGC CCACACCAAG ATCGCGGAGG CCATGCATCA CCAGGACTGC CTGCTGTTCG 
     2401 CCACCAGCCA CCTGATTTCC CTGGAGGTGT CCCCACCACC CACCCTGTCT CAGAACCTGT 
     2461 CGGGCTCTCC ACTCATTACG GTCCACCTCA AGCACAGATT GACACGTAAG CAGCACAGTG 
     2521 AGGCCACCAA CAGCAGCAAC CGAGTCTTCG TGTACTGCGC CTTCCTGGAC TTCAGCTCCG 
     2581 GAGAAGGGGT CTGGTCGAAC CACGGCTGTG CGCTCACGAG AGGAAACCTC ACCTACTCCG 
     2641 TCTGCCGCTG CACTCACCTC ACCAACTTTG CCATCCTCAT GCAGGTGGTC CCGCTGGAGC 
     2701 TTGCACGCGG ACACCAGGTG GCGCTGTCGT CTATCAGCTA TGTGGGCTGC TCCCTCTCCG 
     2761 TGCTCTGCCT GGTGGCCACG CTGGTCACCT TCGCCGTGCT GTCCTCCGTG AGCACCATCC 
     2821 GGAACCAGCG CTACCACATC CACGCCAACC TGTCCTTCGC CGTGCTGGTG GCCCAGGTCC 
     2881 TGCTGCTCAT TAGTTTCCGC CTCGAGCCGG GCACGACCCC CTGCCAAGTG ATGGCCGTGC 
     2941 TCCTACACTA CTTCTTCCTG AGTGCCTTCG CATGGATGCT GGTGGAGGGG CTGCACCTCT 
     3001 ACAGCATGGT GATCAAGGTC TTTGGGTCGG AGGACAGCAA GCACCGTTAC TACTATGGGA 
     3061 TGGGATGGGG TTTTCCTCTT CTGATCTGCA TCATTTCACT GTCATTTGCC ATGGACAGTT 
     3121 ACGGAACAAG CAACAATTGC TGGCTGTCGT TGGCGAGTGG CGCCATCTGG GCCTTTGTAG 
     3181 CCCCTGCTCT GTTTGTCATC GTGGTCAACA TTGGCATCCT CATCGCTGTG ACCAGAGTCA 
     3241 TCTCACAGAT CAGCGCCGAC AACTACAAGA TCCATGGAGA CCCCAGTGCC TTCAAGTTGA 
     3301 CAGCCAAGGC AGTGGCCGTG CTGCTGCCCA TCCTGGGTAC CTCGTGGGTC TTTGGCGTGC 
     3361 TTGCTGTCAA CGGTTGTGCT GTGGTTTTCC AGTACATGTT TGCCACGCTC AACTCCCTGC 
     3421 AGGGACTGTT CATATTCCTC TTTCATTGTC TCCTGAATTC AGAGGTGAGA GCCGCCTTCA 
     3481 AGCACAAAAC CAAGGTCTGG TCGCTCACGA GCAGCTCCGC CCGCACCTCC AACGCGAAGC 
     3541 CCTTCCACTC GGACCTCATG AATGGGACCC GGCCAGGCAT GGCCTCCACC AAGCTCAGCC 
     3601 CTTGGGACAA GAGCAGCCAC TCTGCCCACC GCGTCGACCT GTCAGCCGTG ACGCGTACGC 
     3661 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     3721 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     3781 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     3841 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     3901 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     3961 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     4021 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     4081 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     4141 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     4201 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     4261 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     4321 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     4381 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     4441 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     4501 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     4561 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     4621 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     4681 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     4741 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     4801 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     4861 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     4921 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     4981 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     5041 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     5101 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     5161 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     5221 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     5281 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     5341 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     5401 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     5461 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     5521 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     5581 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     5641 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     5701 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     5761 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     5821 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     5881 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     5941 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     6001 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     6061 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     6121 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     6181 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     6241 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     6301 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     6361 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     6421 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     6481 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     6541 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     6601 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     6661 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     6721 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     6781 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     6841 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     6901 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     6961 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     7021 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     7081 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     7141 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     7201 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     7261 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     7321 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     7381 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     7441 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     7501 TTT
//