LOCUS       RC224085                8628 bp    DNA     circular  SYN 
DEFINITION  BRIP1 (Myc-DDK-tagged)-Human BRCA1 interacting protein C-terminal helicase 1 (BRIP1)
ACCESSION   NM_032043
VERSION     NM_032043.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4785..4876 
                     /label="Myc-DDK"  
     misc_feature    4906..5491 
                     /label="PolyA signal"  
     misc_feature    4963..4982 
                     /label="Restriction Enzyme"  
     misc_feature    5767..6439 
                     /label="ColE1"  
     misc_feature    6869..7663 
                     /label="Kan/Neo"  
     misc_feature    7698..8000 
                     /label="SV40 ori"  
     misc_feature    8086..8563 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4767..4773 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4767 
                     /label="BRIP1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCTTCAATG TGGTCTGAAT ATACAATTGG TGGGGTGAAG ATTTACTTTC 
     1081 CTTATAAAGC TTACCCGTCA CAGCTTGCTA TGATGAATTC TATTCTCAGA GGATTAAACA 
     1141 GCAAGCAACA TTGTTTGTTG GAGAGTCCCA CAGGAAGTGG AAAAAGCTTA GCCTTACTTT 
     1201 GTTCTGCTTT AGCATGGCAA CAATCTCTTA GTGGGAAACC AGCAGATGAG GGCGTAAGTG 
     1261 AAAAAGCTGA AGTACAATTG TCATGTTGTT GTGCATGCCA TTCAAAGGAT TTTACAAACA 
     1321 ATGACATGAA CCAAGGAACT TCACGTCATT TCAACTATCC AAGCACACCA CCTTCTGAAA 
     1381 GAAATGGCAC TTCATCAACT TGTCAAGACT CCCCTGAAAA AACCACTCTG GCTGCAAAGT 
     1441 TATCTGCTAA GAAACAGGCA TCCATATACA GAGATGAAAA TGATGATTTT CAAGTAGAGA 
     1501 AGAAAAGAAT TCGACCCTTA GAAACTACAC AGCAGATTAG AAAACGTCAT TGCTTTGGAA 
     1561 CAGAAGTACA CAATTTGGAT GCAAAAGTTG ATTCAGGAAA GACTGTAAAA CTCAACTCTC 
     1621 CACTGGAAAA GATAAACTCC TTTTCGCCAC AGAAACCCCC TGGCCACTGT TCTAGGTGCT 
     1681 GTTGTTCTAC TAAACAAGGA AACAGTCAAG AGTCATCGAA TACCATTAAG AAGGATCATA 
     1741 CAGGGAAATC CAAGATACCC AAAATATATT TTGGGACACG CACACACAAG CAGATTGCTC 
     1801 AGATTACTAG AGAGCTCCGG AGGACGGCAT ATTCAGGGGT TCCAATGACT ATTCTTTCCA 
     1861 GCAGGGATCA TACTTGTGTC CATCCTGAGG TAGTCGGTAA CTTCAACAGA AATGAGAAGT 
     1921 GCATGGAATT GCTAGATGGG AAAAACGGAA AATCCTGCTA TTTTTATCAT GGAGTTCATA 
     1981 AAATTAGTGA TCAGCACACA TTACAGACTT TCCAAGGGAT GTGCAAAGCC TGGGATATAG 
     2041 AAGAACTTGT CAGCCTGGGG AAGAAACTAA AGGCCTGTCC ATATTACACA GCCCGAGAAC 
     2101 TAATACAAGA TGCTGACATC ATATTTTGTC CCTACAACTA TCTTCTAGAT GCACAAATAA 
     2161 GGGAAAGTAT GGATTTAAAT CTGAAAGAAC AGGTTGTCAT TTTAGATGAA GCTCATAACA 
     2221 TCGAGGACTG TGCTCGGGAA TCAGCAAGTT ACAGTGTAAC AGAAGTTCAG CTTCGGTTTG 
     2281 CTCGGGATGA ACTAGATAGT ATGGTCAACA ATAATATAAG GAAGAAAGAT CATGAACCCC 
     2341 TACGAGCTGT GTGCTGTAGC CTCATTAATT GGTTAGAAGC AAACGCTGAA TATCTTGTAG 
     2401 AAAGAGATTA TGAATCAGCT TGTAAAATAT GGAGTGGAAA TGAAATGCTC TTAACTTTAC 
     2461 ACAAAATGGG TATCACCACT GCTACTTTTC CCATTTTGCA GGGACATTTT TCTGCTGTTC 
     2521 TTCAAAAAGA GGAAAAAATC TCACCAATTT ATGGTAAAGA GGAGGCAAGA GAAGTACCTG 
     2581 TTATTAGTGC ATCAACTCAA ATAATGCTTA AAGGACTTTT TATGGTACTT GACTATCTTT 
     2641 TTAGGCAAAA TAGCAGATTT GCAGATGATT ATAAAATTGC GATTCAACAG ACTTACTCCT 
     2701 GGACAAATCA GATTGATATT TCAGACAAAA ATGGGTTGTT GGTTCTACCA AAAAATAAGA 
     2761 AACGTTCACG ACAGAAAACT GCAGTTCATG TGCTAAACTT TTGGTGCTTA AATCCAGCTG 
     2821 TGGCCTTTTC AGATATTAAT GGCAAAGTTC AGACCATTGT TTTGACATCT GGTACATTAT 
     2881 CACCAATGAA ATCCTTTTCG TCAGAACTTG GTGTTACATT TACTATCCAG CTGGAGGCTA 
     2941 ATCATATCAT TAAAAATTCA CAGGTTTGGG TTGGTACCAT TGGGTCAGGC CCCAAGGGTC 
     3001 GGAATCTCTG TGCTACCTTC CAGAATACTG AAACATTTGA GTTCCAAGAT GAAGTGGGAG 
     3061 CACTTTTGTT ATCTGTGTGC CAGACTGTGA GCCAAGGAAT TTTGTGTTTC TTGCCATCTT 
     3121 ACAAGTTATT AGAAAAATTA AAAGAACGTT GGCTCTCTAC TGGTTTATGG CATAATCTGG 
     3181 AGTTGGTGAA GACAGTCATT GTAGAACCAC AGGGAGGAGA AAAAACAAAT TTTGATGAAT 
     3241 TACTGCAGGT GTACTATGAC GCAATCAAAT ACAAAGGAGA GAAAGATGGA GCTCTCCTGG 
     3301 TAGCAGTTTG TCGTGGTAAA GTGAGTGAGG GTCTGGATTT CTCAGATGAC AATGCCCGTG 
     3361 CTGTCATAAC AATAGGAATT CCTTTTCCAA ATGTGAAAGA TCTACAGGTT GAACTAAAAC 
     3421 GACAATACAA TGACCACCAT TCAAAATTGA GAGGTCTTCT ACCTGGCCGT CAGTGGTATG 
     3481 AAATTCAAGC ATACAGGGCC TTAAACCAGG CCCTTGGTAG ATGTATTAGA CACAGAAATG 
     3541 ATTGGGGAGC TCTTATTCTA GTGGATGATC GCTTTAGGAA TAACCCAAGT CGCTATATAT 
     3601 CTGGACTTTC TAAATGGGTA CGGCAGCAGA TTCAGCACCA TTCAACCTTT GAAAGTGCAC 
     3661 TGGAGTCCTT GGCTGAATTT TCCAAAAAGC ATCAAAAAGT TCTTAATGTA TCCATAAAGG 
     3721 ACAGAACCAA TATACAGGAC AATGAGTCTA CACTTGAAGT GACCTCTTTA AAGTACAGTA 
     3781 CCCCACCTTA TTTACTGGAA GCAGCAAGTC ATCTATCACC AGAAAATTTT GTGGAAGATG 
     3841 AAGCAAAGAT ATGTGTCCAG GAACTACAGT GTCCTAAAAT TATTACCAAA AATTCACCTC 
     3901 TACCAAGTAG CATTATCTCC AGAAAGGAGA AAAATGATCC AGTATTCCTG GAAGAAGCAG 
     3961 GGAAAGCAGA AAAAATTGTG ATTTCCAGAT CCACAAGCCC AACTTTCAAC AAACAAACAA 
     4021 AGAGAGTTAG CTGGTCAAGC TTTAATTCTT TGGGACAGTA TTTTACTGGT AAAATACCGA 
     4081 AGGCAACACC TGAGCTCGGG TCATCAGAGA ATAGTGCCTC TAGTCCTCCC CGTTTCAAAA 
     4141 CAGAGAAGAT GGAAAGTAAA ACTGTTTTGC CCTTCACTGA TAAATGTGAA TCCTCAAATC 
     4201 TGACAGTAAA CACATCGTTT GGATCATGCC CTCAATCAGA AACCATTATT TCATCATTAA 
     4261 AGATTGATGC CACCCTTACT AGAAAAAATC ATTCTGAACA TCCGCTCTGT TCTGAAGAAG 
     4321 CCCTGGATCC AGACATTGAA TTGTCTCTAG TAAGTGAAGA AGATAAACAG TCCACTTCAA 
     4381 ATAGAGATTT TGAAACAGAA GCAGAAGATG AATCTATCTA TTTTACACCT GAACTTTACG 
     4441 ATCCTGAAGA TACAGATGAA GAAAAAAATG ACCTAGCTGA AACTGATAGA GGAAATAGAT 
     4501 TGGCTAACAA TTCAGATTGC ATTTTAGCTA AAGACCTTTT TGAAATTAGA ACTATAAAAG 
     4561 AAGTAGATTC AGCCAGAGAA GTGAAAGCTG AGGATTGCAT AGATACAAAG TTGAATGGAA 
     4621 TTCTGCATAT TGAAGAAAGT AAAATTGATG ACATTGATGG TAATGTAAAA ACAACTTGGA 
     4681 TAAATGAACT GGAACTGGGA AAAACTCATG AAATAGAAAT AAAGAACTTT AAACCATCTC 
     4741 CTTCCAAAAA TAAAGGCATG TTTCCTGGTT TTAAGACGCG TACGCGGCCG CTCGAGCAGA 
     4801 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     4861 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     4921 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     4981 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     5041 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     5101 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     5161 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     5221 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     5281 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     5341 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     5401 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     5461 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     5521 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     5581 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     5641 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     5701 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     5761 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     5821 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     5881 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     5941 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     6001 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     6061 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     6121 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     6181 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     6241 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     6301 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     6361 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     6421 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     6481 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     6541 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     6601 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     6661 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     6721 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     6781 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     6841 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     6901 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     6961 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     7021 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     7081 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     7141 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     7201 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     7261 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     7321 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     7381 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     7441 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     7501 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     7561 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     7621 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     7681 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     7741 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     7801 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     7861 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7921 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     7981 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     8041 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     8101 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     8161 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     8221 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     8281 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     8341 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     8401 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     8461 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     8521 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     8581 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//