LOCUS       RC224076                7428 bp    DNA     circular  SYN 
DEFINITION  RASA2 (Myc-DDK-tagged)-Human RAS p21 protein activator 2 (RASA2)
ACCESSION   NM_006506
VERSION     NM_006506.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3585..3676 
                     /label="Myc-DDK"  
     misc_feature    3706..4291 
                     /label="PolyA signal"  
     misc_feature    3763..3782 
                     /label="Restriction Enzyme"  
     misc_feature    4567..5239 
                     /label="ColE1"  
     misc_feature    5669..6463 
                     /label="Kan/Neo"  
     misc_feature    6498..6800 
                     /label="SV40 ori"  
     misc_feature    6886..7363 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3567..3573 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3567 
                     /label="RASA2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGCGGCG GCGCCTGCTG CTGCGGCGGC TTCTTCCGAG GCGCCAGCGG 
     1081 CGAGTGCGAC TGCAGAGCCC GAGGCCGGGG ACCAGGACAG TCGCGAGGTT CGAGTGTTGC 
     1141 AGAGCCTGCG GGGCAAGATC TGTGAAGCAA AAAATTTATT GCCATATCTT GGACCCCACA 
     1201 AAATGAGAGA TTGTTTCTGT ACCATAAATT TGGACCAGGA AGAAGTTTAT CGTACCCAAG 
     1261 TTGTGGAAAA ATCTTTAAGC CCATTTTTCA GTGAAGAATT TTACTTTGAG ATTCCAAGAA 
     1321 CTTTCCAGTA TTTGTCTTTC TATGTTTATG ATAAGAATGT TTTACAAAGA GATCTCCGTA 
     1381 TAGGAAAAGT AGCCATCAAA AAAGAAGACT TGTGTAATCA CAGTGGCAAA GAAACTTGGT 
     1441 TTTCATTACA GCCTGTTGAC TCCAATTCAG AGGTTCAGGG TAAAGTTCAC CTTGAATTAA 
     1501 AACTGAATGA ACTGATAACG GAGAATGGAA CTGTATGCCA GCAGCTTGTT GTACACATCA 
     1561 AGGCATGCCA TGGGTTGCCT CTCATAAATG GCCAAAGCTG TGACCCTTAT GCAACAGTTT 
     1621 CTCTAGTGGG CCCTTCTAGG AATGACCAAA AGAAGACAAA AGTAAAGAAG AAAACAAGCA 
     1681 ATCCGCAGTT TAATGAAATC TTTTATTTTG AAGTAACCAG ATCCAGTAGT TACACCAGAA 
     1741 AGTCCCAGTT CCAGGTAGAA GAGGAGGACA TTGAAAAGCT AGAAATCAGG ATCGACTTGT 
     1801 GGAACAATGG AAACCTAGTC CAAGATGTTT TCCTAGGTGA GATTAAGGTT CCTGTGAACG 
     1861 TATTAAGAAC TGATTCCTCT CATCAAGCCT GGTACTTGCT ACAGCCAAGA GACAATGGAA 
     1921 ACAAGTCATC CAAAACTGAT GACCTGGGGT CTCTTCGATT AAATATATGT TATACAGAAG 
     1981 ACTACGTGCT TCCTTCAGAG TACTATGGTC CTTTGAAAAC TTTGCTGCTA AAATCACCAG 
     2041 ATGTTCAACC AATATCTGCC TCAGCTGCTT ACATTTTGAG TGAAATATGT CGAGATAAAA 
     2101 ATGATGCTGT TTTGCCCCTT GTACGACTGC TGCTGCACCA TGATAAACTT GTTCCTTTTG 
     2161 CCACTGCTGT GGCTGAATTA GACTTGAAGG ATACACAAGA TGCAAACACA ATTTTTAGAG 
     2221 GAAATTCCCT GGCTACCCGA TGTCTGGATG AGATGATGAA AATAGTGGGA GGGCACTACC 
     2281 TGAAAGTAAC ATTAAAACCT ATTCTTGATG AGATATGTGA CTCCTCAAAA TCCTGTGAAA 
     2341 TCGATCCTAT TAAATTGAAA GAGGGAGATA ATGTAGAAAA TAATAAGGAG AATCTGCGCT 
     2401 ACTATGTAGA CAAGTTATTC AATACAATTG TAAAATCAAG TATGAGCTGC CCCACTGTAA 
     2461 TGTGTGATAT CTTTTATTCT CTAAGGCAGA TGGCTACTCA GAGATTTCCT AATGACCCTC 
     2521 ATGTTCAGTA TTCTGCAGTG AGCAGCTTTG TATTTCTTCG TTTCTTTGCT GTAGCCGTAG 
     2581 TATCACCTCA TACTTTTCAT TTGCGACCTC ATCATCCAGA TGCACAGACA ATTAGAACAT 
     2641 TAACTCTCAT CTCAAAAACT ATACAAACTT TGGGAAGCTG GGGGAGTCTG TCCAAAAGCA 
     2701 AGTCAAGTTT CAAAGAGACA TTCATGTGTG AATTTTTCAA AATGTTTCAA GAAGAAGGAT 
     2761 ATATTATAGC AGTTAAAAAG TTCTTGGATG AAATTTCATC TACTGAAACT AAAGAGTCCA 
     2821 GTGGTACGAG TGAGCCTGTG CACCTGAAAG AAGGTGAGAT GTATAAAAGA GCTCAAGGAA 
     2881 GAACTCGGAT TGGAAAAAAG AATTTTAAGA AACGATGGTT CTGCTTAACA AGCAGAGAGC 
     2941 TCACCTACCA CAAACAGCCA GGCAAAGATG CAATCTACAC AATCCCAGTA AAAAACATTC 
     3001 TTGCTGTGGA AAAACTGGAA GAGAGCTCTT TCAACAAGAA AAATATGTTC CAAGTAATAC 
     3061 ATACGGAGAA ACCACTCTAT GTCCAGGCAA ATAACTGTGT AGAAGCTAAT GAATGGATAG 
     3121 ACGTACTCTG CAGGGTGAGC CGATGCAATC AAAACAGGCT CAGTTTTTAT CATCCCTCTG 
     3181 TGTATCTAAA CGGAAATTGG CTCTGCTGTC AGGAGACTGG TGAAAACACT CTCGGCTGCA 
     3241 AGCCATGTAC TGCAGGTGTC CCTGCAGACA TCCAAATAGA TATTGATGAA GACAGAGAAA 
     3301 CAGAAAGAAT TTATTCCCTT TTTACCCTCA GTTTACTTAA GCTGCAGAAG ATGGAAGAGG 
     3361 CTTGTGGAAC TATTGCAGTC TATCAAGGAC CACAGAAAGA GCCTGATGAT TATTCTAACT 
     3421 TTGTAATCGA GGATTCTGTA ACAACCTTTA AGACAATTCA GCAAATAAAA AGCATAATTG 
     3481 AGAAGCTGGA TGAACCTCAT GAAAAATATA GGAAGAAAAG ATCCAGTAGT GCAAAATATG 
     3541 GGAGCAAGGA AAATCCAATT GTTGGGAAAG CATCTACGCG TACGCGGCCG CTCGAGCAGA 
     3601 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     3661 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     3721 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     3781 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     3841 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     3901 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     3961 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     4021 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     4081 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     4141 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     4201 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     4261 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     4321 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     4381 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     4441 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     4501 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     4561 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     4621 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     4681 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     4741 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     4801 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     4861 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     4921 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     4981 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     5041 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     5101 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     5161 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     5221 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     5281 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     5341 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     5401 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     5461 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     5521 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     5581 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     5641 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     5701 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     5761 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     5821 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     5881 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     5941 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     6001 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     6061 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     6121 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     6181 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     6241 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     6301 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     6361 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     6421 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     6481 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     6541 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     6601 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     6661 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     6721 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     6781 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     6841 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     6901 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     6961 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     7021 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     7081 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     7141 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     7201 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     7261 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     7321 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     7381 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//