LOCUS       RC224070L1                9063 bp    DNA     circular  SYN 
DEFINITION  LPIN2 (Myc-DDK-tagged)-Human lipin 2 (LPIN2)
ACCESSION   NM_014646
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7354..7534 
                     /label="3'LTR"  
     misc_feature    833..1013 
                     /label="5'LTR"  
     misc_feature    8262..8921 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV promoter"  
     misc_feature    7693..8261 
                     /label="ColE1"  
     misc_feature    6229..6249 
                     /label="LR50 primer"  
     misc_feature    6026..6097 
                     /label="Myc-DDK"  
     misc_feature    3252..3271 
                     /label="T7 promoter"  
     misc_feature    3221..3237 
                     /label="V2 primer"  
     misc_feature    6238..6829 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    6008..6014 
                     /label="Restriction Enzyme"  
     misc_feature    3320..6008 
                     /label="LPIN2"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GAATTATGTG GGACAGCTGG CTGGGCAGGT 
     3361 GATTGTCACT GTGAAGGAAC TCTACAAGGG CATTAACCAG GCCACCCTCT CTGGGTGCAT 
     3421 TGATGTCATC GTGGTACAGC AGCAGGATGG CAGCTATCAG TGTTCACCTT TTCACGTTCG 
     3481 GTTTGGAAAG CTGGGAGTCC TGAGATCCAA AGAGAAAGTG ATTGATATAG AAATCAACGG 
     3541 CAGTGCAGTG GATCTTCACA TGAAGTTGGG TGATAACGGA GAAGCTTTCT TTGTTGAGGA 
     3601 GACTGAAGAA GAATATGAAA AGCTTCCTGC TTACCTTGCC ACCTCACCAA TTCCTACTGA 
     3661 AGATCAGTTC TTTAAAGATA TTGACACCCC TTTGGTGAAA TCGGGTGGAG ATGAAACACC 
     3721 ATCTCAGAGT TCAGACATCT CACACGTCTT GGAAACAGAG ACAATTTTTA CTCCAAGTTC 
     3781 TGTGAAAAAG AAAAAACGAA GGAGAAAGAA ATACAAACAG GACAGTAAGA AGGAAGAGCA 
     3841 GGCCGCATCT GCTGCTGCAG AAGACACATG TGATGTAGGC GTGAGCTCCG ATGATGACAA 
     3901 GGGGGCCCAG GCAGCACGAG GATCTTCAAA TGCTTCCTTG AAAGAAGAAG AATGTAAAGA 
     3961 GCCTTTGCTC TTCCATTCTG GGGATCATTA CCCCTTATCT GATGGAGATT GGTCCCCTTT 
     4021 AGAGACCACC TATCCCCAGA CAGCGTGTCC TAAGAGTGAT TCAGAGCTGG AGGTGAAACC 
     4081 TGCGGAGAGC CTGCTCAGAT CAGAGTCTCA CATGGAGTGG ACGTGGGGCG GATTCCCAGA 
     4141 GTCCACCAAG GTCAGCAAAA GAGAACGATC TGACCATCAT CCTAGGACAG CTACAATTAC 
     4201 ACCATCAGAA AATACTCATT TTCGGGTAAT TCCCAGTGAG GACAACCTCA TCAGTGAAGT 
     4261 TGAGAAGGAT GCTTCCATGG AAGACACTGT CTGTACCATA GTGAAGCCCA AACCCAGAGC 
     4321 CCTGGGTACA CAGATGAGCG ACCCAACATC TGTGGCAGAG CTTCTCGAAC CTCCTCTTGA 
     4381 GAGTACTCAG ATTTCATCTA TGTTAGATGC TGACCACCTT CCCAACGCAG CCTTAGCGGA 
     4441 GGCGCCCTCA GAATCCAAAC CGGCAGCTAA AGTAGACTCG CCGTCAAAGA AGAAAGGTGT 
     4501 TCACAAAAGA AGCCAACACC AGGGACCTGA TGATATTTAC CTTGATGACT TAAAGGGTCT 
     4561 AGAACCTGAA GTTGCAGCTC TTTATTTCCC TAAAAGTGAA TCGGAGCCCG GTTCCAGGCA 
     4621 GTGGCCCGAG TCTGACACAC TCTCTGGCTC CCAGTCCCCA CAGTCCGTGG GAAGCGCAGC 
     4681 TGCAGATAGC GGCACCGAGT GCCTCTCAGA TTCTGCCATG GACTTGCCTG ACGTTACCCT 
     4741 CTCCCTTTGC GGGGGCCTCA GTGAAAATGG AGAAATTTCA AAAGAAAAAT TCATGGAGCA 
     4801 TATCATTACT TATCACGAAT TTGCAGAAAA CCCTGGACTT ATAGACAATC CTAACCTTGT 
     4861 AATAAGGATA TATAATCGTT ACTATAACTG GGCTTTGGCA GCTCCCATGA TCCTTAGCTT 
     4921 GCAAGTATTC CAGAAGAGCT TGCCTAAGGC CACAGTTGAG TCCTGGGTGA AAGACAAGAT 
     4981 GCCAAAGAAA TCTGGTCGCT GGTGGTTTTG GCGAAAGAGA GAAAGCATGA CCAAACAGCT 
     5041 GCCAGAATCC AAGGAGGGAA AATCTGAGGC ACCGCCAGCC AGTGACCTGC CATCCAGCTC 
     5101 CAAGGAGCCG GCCGGTGCCA GGCCGGCCGA GAATGACTCC TCGAGTGACG AGGGATCACA 
     5161 GGAGCTCGAA GAATCCATCA CAGTGGACCC CATCCCCACA GAGCCCCTGA GCCACGGCAG 
     5221 CACAACTTCA TATAAGAAGT CTCTCCGCCT CTCCTCAGAC CAGATCGCAA AACTGAAGCT 
     5281 CCACGATGGC CCAAATGATG TTGTGTTTAG TATTACAACC CAGTATCAAG GCACCTGTCG 
     5341 CTGTGCAGGG ACCATTTACC TGTGGAACTG GAATGACAAG ATCATCATTT CTGATATTGA 
     5401 TGGGACAATA ACCAAGTCGG ATGCTTTGGG ACAGATTCTC CCACAGCTGG GCAAAGACTG 
     5461 GACCCACCAG GGTATAGCAA AGCTCTACCA TTCCATCAAT GAGAATGGCT ACAAGTTTCT 
     5521 GTACTGCTCG GCTCGTGCCA TCGGCATGGC CGACATGACC CGTGGCTACC TGCACTGGGT 
     5581 CAATGACAAG GGCACAATCT TGCCCCGGGG CCCCCTGATG CTGTCCCCCA GCAGCTTGTT 
     5641 CTCCGCCTTC CACAGAGAAG TGATAGAAAA GAAACCAGAG AAGTTCAAAA TTGAGTGTCT 
     5701 AAATGATATC AAGAATCTGT TTGCCCCGTC TAAGCAGCCC TTCTATGCTG CCTTTGGAAA 
     5761 CCGTCCAAAT GATGTCTATG CCTACACACA AGTTGGAGTT CCAGACTGTA GAATATTCAC 
     5821 CGTGAACCCC AAGGGTGAAT TAATACAAGA AAGAACCAAA GGAAACAAGT CATCGTATCA 
     5881 CAGGCTGAGT GAGCTCGTGG AGCATGTGTT CCCCCTTCTC AGTAAGGAGC AGAATTCCGC 
     5941 TTTTCCCTGC CCGGAGTTCA GCTCCTTCTG CTACTGGCGA GACCCGATCC CTGAAGTGGA 
     6001 CCTGGATGAC CTGTCTACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA 
     6061 TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG 
     6121 CCGCGGTCTG TACAAGTAGG ATTCGTCGAG GGACCTAATA ACTTCGTATA GCATACATTA 
     6181 TACGAAGTTA TACATGTTTA AGGGTTCCGG TTCCACTAGG TACAATTCGA TATCAAGCTT 
     6241 ATCGATAATC AACCTCTGGA TTACAAAATT TGTGAAAGAT TGACTGGTAT TCTTAACTAT 
     6301 GTTGCTCCTT TTACGCTATG TGGATACGCT GCTTTAATGC CTTTGTATCA TGCTATTGCT 
     6361 TCCCGTATGG CTTTCATTTT CTCCTCCTTG TATAAATCCT GGTTGCTGTC TCTTTATGAG 
     6421 GAGTTGTGGC CCGTTGTCAG GCAACGTGGC GTGGTGTGCA CTGTGTTTGC TGACGCAACC 
     6481 CCCACTGGTT GGGGCATTGC CACCACCTGT CAGCTCCTTT CCGGGACTTT CGCTTTCCCC 
     6541 CTCCCTATTG CCACGGCGGA ACTCATCGCC GCCTGCCTTG CCCGCTGCTG GACAGGGGCT 
     6601 CGGCTGTTGG GCACTGACAA TTCCGTGGTG TTGTCGGGGA AATCATCGTC CTTTCCTTGG 
     6661 CTGCTCGCCT GTGTTGCCAC CTGGATTCTG CGCGGGACGT CCTTCTGCTA CGTCCCTTCG 
     6721 GCCCTCAATC CAGCGGACCT TCCTTCCCGC GGCCTGCTGC CGGCTCTGCG GCCTCTTCCG 
     6781 CGTCTTCGCC TTCGCCCTCA GACGAGTCGG ATCTCCCTTT GGGCCGCCTC CCCGCATCGA 
     6841 TACCGTCGAC CTCGATCGAG ACCTAGAAAA ACATGGAGCA ATCACAAGTA GCAATACAGC 
     6901 AGCTACCAAT GCTGATTGTG CCTGGCTAGA AGCACAAGAG GAGGAGGAGG TGGGTTTTCC 
     6961 AGTCACACCT CAGGTACCTT TAAGACCAAT GACTTACAAG GCAGCTGTAG ATCTTAGCCA 
     7021 CTTTTTAAAA GAAAAGGGGG GACTGGAAGG GCTAATTCAC TCCCAACGAA GACAAGATAT 
     7081 CCTTGATCTG TGGATCTACC ACACACAAGG CTACTTCCCT GATTGGCAGA ACTACACACC 
     7141 AGGGCCAGGG ATCAGATATC CACTGACCTT TGGATGGTGC TACAAGCTAG TACCAGTTGA 
     7201 GCAAGAGAAG GTAGAAGAAG CCAATGAAGG AGAGAACACC CGCTTGTTAC ACCCTGTGAG 
     7261 CCTGCATGGG ATGGATGACC CGGAGAGAGA AGTATTAGAG TGGAGGTTTG ACAGCCGCCT 
     7321 AGCATTTCAT CACATGGCCC GAGAGCTGCA TCCGGACTGT ACTGGGTCTC TCTGGTTAGA 
     7381 CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA AGCCTCAATA 
     7441 AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT CTGGTAACTA 
     7501 GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGCATGT GAGCAAAAGG 
     7561 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     7621 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     7681 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     7741 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     7801 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     7861 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     7921 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     7981 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     8041 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     8101 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     8161 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     8221 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA TTACGCCCCG 
     8281 CCCTGCCACT CATCGCAGTA CTGTTGTAAT TCATTAAGCA TTCTGCCGAC ATGGAAGCCA 
     8341 TCACAAACGG CATGATGAAC CTGAATCGCC AGCGGCATCA GCACCTTGTC GCCTTGCGTA 
     8401 TAATATTTGC CCATGGTGAA AACGGGGGCG AAGAAGTTGT CCATATTGGC CACGTTTAAA 
     8461 TCAAAACTGG TGAAACTCAC CCAGGGATTG GCTGAGACGA AAAACATATT CTCAATAAAC 
     8521 CCTTTAGGGA AATAGGCCAG GTTTTCACCG TAACACGCCA CATCTTGCGA ATATATGTGT 
     8581 AGAAACTGCC GGAAATCGTC GTGGTATTCA CTCCAGAGCG ATGAAAACGT TTCAGTTTGC 
     8641 TCATGGAAAA CGGTGTAACA AGGGTGAACA CTATCCCATA TCACCAGCTC ACCGTCTTTC 
     8701 ATTGCCATAC GGAACTCCGG ATGAGCATTC ATCAGGCGGG CAAGAATGTG AATAAAGGCC 
     8761 GGATAAAACT TGTGCTTATT TTTCTTTACG GTCTTTAAAA AGGCCGTAAT ATCCAGCTGA 
     8821 ACGGTCTGGT TATAGGTACA TTGAGCAACT GACTGAAATG CCTCAAAATG TTCTTTACGA 
     8881 TGCCATTGGG ATATATCAAC GGTGGTATAT CCAGTGATTT TTTTCTCCAT ACTCTTCCTT 
     8941 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 
     9001 TGTATTTAGA AAAATAAACA AATAGGGGTC CCGCGCACAT TTCCCCGAAA AGTGCCACCT 
     9061 GAC
//