LOCUS       RC224053                8517 bp    DNA     circular  SYN 
DEFINITION  SLC12A2 (Myc-DDK-tagged)-Human solute carrier family 12 (sodium/potassium/chloride transporters), member 2 (SLC12A2)
ACCESSION   NM_001046
VERSION     NM_001046.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4674..4765 
                     /label="Myc-DDK"  
     misc_feature    4795..5380 
                     /label="PolyA signal"  
     misc_feature    4852..4871 
                     /label="Restriction Enzyme"  
     misc_feature    5656..6328 
                     /label="ColE1"  
     misc_feature    6758..7552 
                     /label="Kan/Neo"  
     misc_feature    7587..7889 
                     /label="SV40 ori"  
     misc_feature    7975..8452 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4656..4662 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4656 
                     /label="SLC12A2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGCCGCGG CCCACGGCGC CCTCCTCCGG CGCCCCGGGA CTGGCCGGGG 
     1081 TCGGGGAGAC GCCGTCAGCC GCTGCGCTGG CCGCAGCCAG GGTGGAACTG CCCGGCACGG 
     1141 CTGTGCCCTC GGTGCCGGAG GATGCTGCGC CCGCGAGCCG GGACGGCGGC GGGGTCCGCG 
     1201 ATGAGGGCCC CGCGGCGGCC GGGGACGGGC TGGGCAGACC CTTGGGGCCC ACCCCGAGCC 
     1261 AGAGCCGTTT CCAGGTGGAC CTGGTTTCCG AGAACGCCGG GCGGGCCGCT GCTGCGGCGG 
     1321 CGGCGGCGGC GGCGGCAGCG GCGGCGGCTG GTGCTGGGGC GGGGGCCAAG CAGACCCCCG 
     1381 CGGACGGGGA AGCCAGCGGC GAGAGCGAGC CGGCTAAAGG CAGCGAGGAA GCCAAGGGCC 
     1441 GCTTCCGCGT GAACTTCGTG GACCCAGCTG CCTCCTCGTC GGCTGAAGAC AGCCTGTCAG 
     1501 ATGCTGCCGG GGTCGGAGTC GACGGGCCCA ACGTGAGCTT CCAGAACGGC GGGGACACGG 
     1561 TGCTGAGCGA GGGCAGCAGC CTGCACTCCG GCGGCGGCGG CGGCAGTGGG CACCACCAGC 
     1621 ACTACTATTA TGATACCCAC ACCAACACCT ACTACCTGCG CACCTTCGGC CACAACACCA 
     1681 TGGACGCTGT GCCCAGGATC GATCACTACC GGCACACAGC CGCGCAGCTG GGCGAGAAGC 
     1741 TGCTCCGGCC TAGCCTGGCG GAGCTCCACG ACGAGCTGGA AAAGGAACCT TTTGAGGATG 
     1801 GCTTTGCAAA TGGGGAAGAA AGTACTCCAA CCAGAGATGC TGTGGTCACG TATACTGCAG 
     1861 AAAGTAAAGG AGTCGTGAAG TTTGGCTGGA TCAAGGGTGT ATTAGTACGT TGTATGTTAA 
     1921 ACATTTGGGG TGTGATGCTT TTCATTAGAT TGTCATGGAT TGTGGGTCAA GCTGGAATAG 
     1981 GTCTATCAGT CCTTGTAATA ATGATGGCCA CTGTTGTGAC AACTATCACA GGATTGTCTA 
     2041 CTTCAGCAAT AGCAACTAAT GGATTTGTAA GAGGAGGAGG AGCATATTAT TTAATATCTA 
     2101 GAAGTCTAGG GCCAGAATTT GGTGGTGCAA TTGGTCTAAT CTTCGCCTTT GCCAACGCTG 
     2161 TTGCAGTTGC TATGTATGTG GTTGGATTTG CAGAAACCGT GGTGGAGTTG CTTAAGGAAC 
     2221 ATTCCATACT TATGATAGAT GAAATCAATG ATATCCGAAT TATTGGAGCC ATTACAGTCG 
     2281 TGATTCTTTT AGGTATCTCA GTAGCTGGAA TGGAGTGGGA AGCAAAAGCT CAGATTGTTC 
     2341 TTTTGGTGAT CCTACTTCTT GCTATTGGTG ATTTCGTCAT AGGAACATTT ATCCCACTGG 
     2401 AGAGCAAGAA GCCAAAAGGG TTTTTTGGTT ATAAATCTGA AATATTTAAT GAGAACTTTG 
     2461 GGCCCGATTT TCGAGAGGAA GAGACTTTCT TTTCTGTATT TGCCATCTTT TTTCCTGCTG 
     2521 CAACTGGTAT TCTGGCTGGA GCAAATATCT CAGGTGATCT TGCAGATCCT CAGTCAGCCA 
     2581 TACCCAAAGG AACACTCCTA GCCATTTTAA TTACTACATT GGTTTACGTA GGAATTGCAG 
     2641 TATCTGTAGG TTCTTGTGTT GTTCGAGATG CCACTGGAAA CGTTAATGAC ACTATCGTAA 
     2701 CAGAGCTAAC AAACTGTACT TCTGCAGCCT GCAAATTAAA CTTTGATTTT TCATCTTGTG 
     2761 AAAGCAGTCC TTGTTCCTAT GGCCTAATGA ACAACTTCCA GGTAATGAGT ATGGTGTCAG 
     2821 GATTTACACC ACTAATTTCT GCAGGTATAT TTTCAGCCAC TCTTTCTTCA GCATTAGCAT 
     2881 CCCTAGTGAG TGCTCCCAAA ATATTTCAGG CTCTATGTAA GGACAACATC TACCCAGCTT 
     2941 TCCAGATGTT TGCTAAAGGT TATGGGAAAA ATAATGAACC TCTTCGTGGC TACATCTTAA 
     3001 CATTCTTAAT TGCACTTGGA TTCATCTTAA TTGCTGAACT GAATGTTATT GCACCAATTA 
     3061 TCTCAAACTT CTTCCTTGCA TCATATGCAT TGATCAATTT TTCAGTATTC CATGCATCAC 
     3121 TTGCAAAATC TCCAGGATGG CGTCCTGCAT TCAAATACTA CAACATGTGG ATATCACTTC 
     3181 TTGGAGCAAT TCTTTGTTGC ATAGTAATGT TCGTCATTAA CTGGTGGGCT GCATTGCTAA 
     3241 CATATGTGAT AGTCCTTGGG CTGTATATTT ATGTTACCTA CAAAAAACCA GATGTGAATT 
     3301 GGGGATCCTC TACACAAGCC CTGACTTACC TGAATGCACT GCAGCATTCA ATTCGTCTTT 
     3361 CTGGAGTGGA AGACCACGTG AAAAACTTTA GGCCACAGTG TCTTGTTATG ACAGGTGCTC 
     3421 CAAACTCACG TCCAGCTTTA CTTCATCTTG TTCATGATTT CACAAAAAAT GTTGGTTTGA 
     3481 TGATCTGTGG CCATGTACAT ATGGGTCCTC GAAGACAAGC CATGAAAGAG ATGTCCATCG 
     3541 ATCAAGCCAA ATATCAGCGA TGGCTTATTA AGAACAAAAT GAAGGCATTT TATGCTCCAG 
     3601 TACATGCAGA TGACTTGAGA GAAGGTGCAC AGTATTTGAT GCAGGCTGCT GGTCTTGGTC 
     3661 GTATGAAGCC AAACACACTT GTCCTTGGAT TTAAGAAAGA TTGGTTGCAA GCAGATATGA 
     3721 GGGATGTGGA TATGTATATA AACTTATTTC ATGATGCTTT TGACATACAA TATGGAGTAG 
     3781 TGGTTATTCG CCTAAAAGAA GGTCTGGATA TATCTCATCT TCAAGGACAA GAAGAATTAT 
     3841 TGTCATCACA AGAGAAATCT CCTGGCACCA AGGATGTGGT AGTAAGTGTG GAATATAGTA 
     3901 AAAAGTCCGA TTTAGATACT TCCAAACCAC TCAGTGAAAA ACCAATTACA CACAAAGTTG 
     3961 AGGAAGAGGA TGGCAAGACT GCAACTCAAC CACTGTTGAA AAAAGAATCC AAAGGCCCTA 
     4021 TTGTGCCTTT AAATGTAGCT GACCAAAAGC TTCTTGAAGC TAGTACACAG TTTCAGAAAA 
     4081 AACAAGGAAA GAATACTATT GATGTCTGGT GGCTTTTTGA TGATGGAGGT TTGACCTTAT 
     4141 TGATACCTTA CCTTCTGACG ACCAAGAAAA AATGGAAAGA CTGTAAGATC AGAGTATTCA 
     4201 TTGGTGGAAA GATAAACAGA ATAGACCATG ACCGGAGAGC GATGGCTACT TTGCTTAGCA 
     4261 AGTTCCGGAT AGACTTTTCT GATATCATGG TTCTAGGAGA TATCAATACC AAACCAAAGA 
     4321 AAGAAAATAT TATAGCTTTT GAGGAAATCA TTGAGCCATA CAGACTTCAT GAAGATGATA 
     4381 AAGAGCAAGA TATTGCAGAT AAAATGAAAG AAGATGAACC ATGGCGAATA ACAGATAATG 
     4441 AGCTTGAACT TTATAAGACC AAGACATACC GGCAGATCAG GTTAAATGAG TTATTAAAGG 
     4501 AACATTCAAG CACAGCTAAT ATTATTGTCA TGAGTCTCCC AGTTGCACGA AAAGGTGCTG 
     4561 TGTCTAGTGC TCTCTACATG GCATGGTTAG AAGCTCTATC TAAGGACCTA CCACCAATCC 
     4621 TCCTAGTTCG TGGGAATCAT CAGAGTGTCC TTACCTTCTA TTCAACGCGT ACGCGGCCGC 
     4681 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     4741 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     4801 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     4861 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     4921 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     4981 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     5041 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     5101 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     5161 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     5221 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     5281 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     5341 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     5401 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     5461 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     5521 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     5581 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     5641 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     5701 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     5761 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     5821 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     5881 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     5941 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     6001 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     6061 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     6121 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     6181 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     6241 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     6301 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     6361 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     6421 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     6481 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     6541 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     6601 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     6661 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     6721 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     6781 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     6841 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     6901 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     6961 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     7021 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     7081 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     7141 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     7201 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     7261 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     7321 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     7381 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     7441 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     7501 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     7561 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     7621 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     7681 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     7741 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7801 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     7861 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     7921 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     7981 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     8041 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     8101 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     8161 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     8221 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     8281 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     8341 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     8401 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     8461 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//