LOCUS       RC224006                8559 bp    DNA     circular  SYN 
DEFINITION  CACHD1 (Myc-DDK-tagged)-Human cache domain containing 1 (CACHD1)
ACCESSION   NM_020925
VERSION     NM_020925.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4714..4805 
                     /label="Myc-DDK"  
     misc_feature    4835..5420 
                     /label="PolyA signal"  
     misc_feature    4892..4911 
                     /label="Restriction Enzyme"  
     misc_feature    5696..6368 
                     /label="ColE1"  
     misc_feature    6798..7592 
                     /label="Kan/Neo"  
     misc_feature    7627..7929 
                     /label="SV40 ori"  
     misc_feature    8015..8492 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4689..4696 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4689 
                     /label="CACHD1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCGGAGGCTG GCGGCCGAGG AGCTGGGGGT CGTCACCATG CAGCGGATAT 
     1081 TCAACTCCTT TGTTTACACT GAGAAAATCT CAAATGGAGA AAGTGAAGTA CAGCAGCTAG 
     1141 CCAAAAAAAT CCGAGAGAAG TTCAACCGTT ACTTGGATGT GGTCAATCGG AACAAGCAAG 
     1201 TTGTAGAAGC ATCCTATACG GCTCACCTAA CCTCTCCCCT AACTGCAATT CAAGACTGCT 
     1261 GTACTATCCC ACCTTCCATG ATGGAATTCG ATGGGAACTT TAATACCAAT GTGTCTAGAA 
     1321 CAATTAGTTG TGATCGACTT TCTACTACTG TTAATAGCCG GGCCTTCAAT CCAGGACGAG 
     1381 ACTTAAATTC AGTTCTTGCA GACAACCTGA AATCCAACCC TGGAATTAAG TGGCAATATT 
     1441 TCAGTTCAGA AGAAGGAATT TTCACTGTTT TCCCAGCACA CAAGTTCCGG TGTAAGGGCA 
     1501 GCTACGAACA CCGCAGTAGA CCCATCTACG TCTCTACAGT CCGGCCGCAG TCAAAGCACA 
     1561 TAGTAGTGAT TCTGGACCAC GGGGCTTCAG TCACAGACAC TCAGCTTCAG ATTGCCAAGG 
     1621 ACGCTGCTCA GGTCATCCTC AGCGCCATCG ATGAACATGA CAAGATTTCT GTGTTAACTG 
     1681 TGGCAGATAC CGTCCGGACT TGCTCACTAG ACCAGTGCTA TAAGACCTTC TTGTCTCCAG 
     1741 CCACCAGTGA GACAAAAAGG AAAATGTCCA CCTTTGTTAG CAGCGTGAAG TCTTCAGACA 
     1801 GTCCTACCCA GCACGCAGTG GGATTCCAAA AGGCATTTCA GCTGATTCGA AGTACAAACA 
     1861 ATAACACAAA GTTCCAAGCA AATACAGACA TGGTCATCAT TTACCTGTCA GCTGGCATTA 
     1921 CATCAAAGGA CTCTTCGGAA GAAGATAAAA AAGCGACTCT CCAAGTCATC AATGAAGAAA 
     1981 ATAGCTTTCT AAACAACTCT GTAATGATTC TCACCTATGC CCTCATGAAC GATGGGGTGA 
     2041 CTGGTTTGAA AGAGCTGGCT TTTCTGAGGG ATCTAGCTGA ACAGAATTCA GGGAAGTACG 
     2101 GTGTGCCAGA CCGGATGGCC TTGCCTGTGA TTAAGGGCAG CATGATGGTG CTGAATCAGT 
     2161 TGAGCAACCT GGAGACCACA GTGGGCAGGT TCTACACAAA CCTTCCCAAC CGGATGATTG 
     2221 ATGAAGCCGT CTTCAGCCTG CCCTTCTCTG ATGAGATGGG AGATGGTTTG ATAATGACTG 
     2281 TGAGTAAACC CTGTTATTTT GGAAACCTAC TTCTGGGAAT TGTAGGTGTG GACGTGAATC 
     2341 TGGCTTACAT TCTTGAAGAC GTGACGTATT ACCAAGACTC TTTGGCTTCC TATACTTTTC 
     2401 TCATAGACGA CAAAGGATAT ACACTTATGC ACCCATCTCT TACCAGGCCA TATTTATTGT 
     2461 CAGAGCCCCC ACTTCATACT GACATCATAC ATTATGAAAA TATTCCAAAA TTTGAATTAG 
     2521 TTCGGCAAAA TATCCTAAGC CTCCCTCTGG GCAGCCAGAT TATCGCAGTC CCTGTGAACT 
     2581 CATCCCTGTC TTGGCACATA AACAAGCTGA GAGAAACTGG AAAGGAAGCC TACAATGTTA 
     2641 GCTATGCCTG GAAGATGGTA CAAGACACTT CCTTTATTCT GTGTATTGTG GTGATACAAC 
     2701 CAGAAATACC TGTGAAACAA CTGAAGAACC TCAACACTGT TCCCAGCAGC AAGCTGCTGT 
     2761 ACCACCGGCT GGATCTCCTT GGCCAGCCCA GTGCTTGCCT CCACTTCAAA CAGCTGGCAA 
     2821 CCCTAGAAAG TCCCACCATC ATGCTGTCTG CTGGCAGCTT TTCCTCCCCC TATGAGCACC 
     2881 TCAGCCAGCC AGAGACAAAG CGCATGGTAG AGCACTACAC CGCCTATCTC AGCGACAACA 
     2941 CCCGCCTCAT TGCTAACCCG GGCCTCAAAT TCTCTGTCAG AAATGAAGTA ATGGCTACCA 
     3001 GCCACGTCAC AGATGAATGG ATGACACAAA TGGAAATGAG TAGCCTGAAC ACTTACATTG 
     3061 TCCGCCGTTA CATAGCAACA CCCAATGGCG TCCTCAGAAT TTATCCTGGT TCCCTCATGG 
     3121 ACAAAGCATT TGATCCCACT AGGAGACAAT GGTATCTCCA TGCAGTAGCT AATCCAGGGT 
     3181 TGATTTCTTT GACTGGTCCT TACTTAGATG TTGGAGGAGC TGGTTATGTT GTGACAATCA 
     3241 GTCACACAAT TCATTCATCC AGTACACAGC TGTCTTCTGG GCACACTGTG GCTGTGATGG 
     3301 GCATTGACTT CACACTCAGA TACTTCTACA AAGTTCTGAT GGACCTATTA CCTGTCTGTA 
     3361 ACCAAGATGG TGGCAACAAA ATAAGGTGCT TCATAATGGA GGACAGGGGT TATCTGGTGG 
     3421 CGCACCCGAC TCTCATCGAC CCCAAAGGAC ATGCACCTGT GGAGCAGCAG CACATCACCC 
     3481 ACAAGGAGCC CCTGGTAGCA AATGATATCC TCAACCACCC CAACTTTGTA AAGAAAAACC 
     3541 TGTGCAACAG CTTCAGTGAC AGAACGGTCC AGAGGTTTTA TAAATTCAAC ACCAGCCTTG 
     3601 CGGGGGATTT GACGAACCTT GTGCATGGCA GCCACTGTTC CAAATACAGA TTAGCAAGGA 
     3661 TCCCAGGAAC CAACGCGTTT GTTGGCATTG TCAACGAAAC CTGCGACTCT CTTGCCTTCT 
     3721 GTGCCTGCAG CATGGTGGAC CGACTCTGTC TCAACTGTCA CCGAATGGAA CAAAATGAAT 
     3781 GTGAATGTCC TTGTGAGTGC CCTCTAGAGG TCAATGAGTG CACTGGCAAC CTCACCAATG 
     3841 CAGAGAACCG AAACCCCAGC TGCGAGGTCC ACCAGGAGCC GGTGACATAC ACAGCTATTG 
     3901 ACCCTGGCCT GCAAGATGCT CTTCACCAGT GTGTCAACAG CAGGTGCAGT CAGAGGCTGG 
     3961 AAAGTGGGGA CTGTTTTGGG GTGCTGGATT GTGAATGGTG CATGGTGGAC AGTGATGGAA 
     4021 AGACTCACCT GGACAAACCC TACTGTGCCC CCCAGAAAGA ATGCTTCGGG GGGATTGTGG 
     4081 GAGCCAAAAG TCCCTACGTT GATGACATGG GAGCAATAGG TGATGAGGTG ATCACATTAA 
     4141 ACATGATTAA AAGCGCCCCT GTGGGTCCTG TGGCTGGAGG GATCATGGGA TGCATCATGG 
     4201 TCTTGGTCCT GGCGGTGTAT GCCTACCGCC ACCAGATTCA TCGCCGGAGC CATCAGCATA 
     4261 TGTCTCCTCT TGCTGCCCAA GAAATGTCAG TGCGTATGTC CAACCTGGAG AATGACAGAG 
     4321 ATGAAAGGGA CGACGACAGC CACGAAGACA GAGGCATCAT CAGCAACACT CGGTTTATAG 
     4381 CTGCGGTCAT CGAACGACAT GCACACAGTC CAGAAAGAAG GCGCCGCTAC TGGGGTCGAT 
     4441 CAGGAACAGA AAGTGATCAT GGTTACAGCA CCATGAGCCC ACAGGAGGAC AGTGAAAATC 
     4501 CTCCATGCAA CAATGACCCC TTGTCAGCCG GGGTCGATGT GGGAAACCAT GATGAGGACT 
     4561 TAGACCTGGA TACCCCCCCT CAGACTGCTG CCCTACTAAG TCACAAGTTC CACCACTACC 
     4621 GGTCACACCA CCCTACACTT CATCATAGCC ACCACTTACA GGCGGCCGTC ACGGTACACA 
     4681 CTGTCGATGC AGAATGCAGC GGACCGACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     4741 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     4801 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     4861 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     4921 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     4981 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     5041 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     5101 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     5161 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     5221 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     5281 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     5341 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     5401 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     5461 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     5521 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     5581 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     5641 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     5701 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     5761 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     5821 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     5881 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     5941 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     6001 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     6061 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     6121 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     6181 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     6241 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     6301 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     6361 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     6421 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     6481 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     6541 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     6601 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     6661 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     6721 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     6781 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     6841 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     6901 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     6961 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     7021 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     7081 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     7141 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     7201 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     7261 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     7321 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     7381 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     7441 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     7501 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     7561 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     7621 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     7681 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     7741 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     7801 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7861 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7921 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     7981 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     8041 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     8101 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     8161 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     8221 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     8281 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     8341 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     8401 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     8461 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     8521 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//