LOCUS       RC224001                9201 bp    DNA     circular  SYN 
DEFINITION  NCOA1 (Myc-DDK-tagged)-Human nuclear receptor coactivator 1 (NCOA1), transcript variant 3
ACCESSION   NM_147233
VERSION     NM_147233.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5358..5449 
                     /label="Myc-DDK"  
     misc_feature    5479..6064 
                     /label="PolyA signal"  
     misc_feature    5536..5555 
                     /label="Restriction Enzyme"  
     misc_feature    6340..7012 
                     /label="ColE1"  
     misc_feature    7442..8236 
                     /label="Kan/Neo"  
     misc_feature    8271..8573 
                     /label="SV40 ori"  
     misc_feature    8659..9136 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5340..5346 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5340 
                     /label="NCOA1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGTGGCCTC GGGGACAGTT CATCCGACCC TGCTAACCCA GACTCACATA 
     1081 AGAGGAAAGG ATCGCCATGT GACACACTGG CATCAAGCAC GGAAAAGAGG CGCAGGGAGC 
     1141 AAGAAAATAA ATATTTAGAA GAACTAGCTG AGTTACTGTC TGCCAACATT AGTGACATTG 
     1201 ACAGCTTGAG TGTAAAACCA GACAAATGCA AGATTTTGAA GAAAACAGTC GATCAGATAC 
     1261 AGCTAATGAA GAGAATGGAA CAAGAGAAAT CAACAACTGA TGACGATGTA CAGAAATCAG 
     1321 ACATCTCATC AAGTAGTCAA GGAGTGATAG AAAAGGAATC CTTGGGACCT CTTCTTTTGG 
     1381 AGGCTTTGGA TGGATTTTTC TTTGTTGTGA ACTGTGAAGG GAGAATTGTA TTTGTGTCAG 
     1441 AGAATGTAAC CAGCTACTTA GGTTACAATC AGGAGGAATT AATGAATACG AGCGTCTACA 
     1501 GCATACTGCA CGTGGGGGAT CATGCAGAAT TTGTGAAGAA TCTGCTACCA AAATCACTAG 
     1561 TAAATGGAGT TCCTTGGCCT CAAGAGGCAA CACGACGAAA TAGCCATACC TTTAACTGCA 
     1621 GGATGCTAAT TCACCCTCCA GATGAGCCAG GGACCGAGAA CCAAGAAGCT TGCCAGCGTT 
     1681 ATGAAGTAAT GCAGTGTTTC ACTGTGTCAC AGCCAAAATC AATTCAAGAG GATGGAGAAG 
     1741 ATTTCCAGTC ATGTCTGATT TGTATTGCAC GGCGATTACC TCGGCCTCCA GCTATTACGG 
     1801 GTGTAGAATC CTTTATGACC AAGCAAGATA CTACAGGTAA AATCATCTCT ATTGATACTA 
     1861 GTTCCCTGAG AGCTGCTGGC AGAACTGGTT GGGAAGATTT AGTGAGGAAG TGCATTTATG 
     1921 CTTTTTTCCA ACCTCAGGGC AGAGAACCAT CTTATGCCAG ACAGCTGTTC CAAGAAGTGA 
     1981 TGACTCGTGG CACTGCCTCC AGCCCCTCCT ATAGATTCAT ATTGAATGAT GGGACAATGC 
     2041 TTAGCGCCCA CACCAAGTGT AAACTTTGCT ACCCTCAAAG TCCAGACATG CAACCTTTCA 
     2101 TCATGGGAAT TCATATCATC GACAGGGAGC ACAGTGGGCT TTCTCCTCAA GATGACACTA 
     2161 ATTCTGGAAT GTCAATTCCC CGAGTAAATC CCTCGGTCAA TCCTAGTATC TCTCCAGCTC 
     2221 ATGGTGTGGC TCGTTCATCC ACATTGCCAC CATCCAACAG CAACATGGTA TCCACCAGAA 
     2281 TAAACCGCCA GCAGAGCTCA GACCTTCATA GCAGCAGTCA TAGTAATTCT AGCAACAGCC 
     2341 AAGGAAGTTT CGGATGCTCA CCCGGAAGTC AGATTGTAGC CAATGTTGCC TTAAACCAAG 
     2401 GACAGGCCAG TTCACAGAGC AGTAATCCCT CTTTAAACCT CAATAATTCT CCTATGGAAG 
     2461 GTACAGGAAT ATCCCTAGCA CAGTTCATGT CTCCAAGGAG ACAGGTTACT TCTGGATTGG 
     2521 CAACAAGGCC CAGGATGCCA AACAATTCCT TTCCTCCTAA TATTTCGACA TTAAGCTCTC 
     2581 CCGTTGGCAT GACAAGTAGT GCCTGTAATA ATAATAACCG ATCTTATTCA AACATCCCAG 
     2641 TAACATCTTT ACAGGGTATG AATGAAGGAC CCAATAACTC CGTTGGCTTC TCTGCCAGTT 
     2701 CTCCAGTCCT CAGGCAGATG AGCTCACAGA ATTCACCTAG CAGATTAAAT ATACAACCAG 
     2761 CAAAAGCTGA GTCCAAAGAT AACAAAGAGA TTGCCTCAAT TTTAAATGAA ATGATTCAAT 
     2821 CTGACAACAG CTCTAGTGAT GGCAAACCTC TGGATTCAGG GCTTCTGCAT AACAATGACA 
     2881 GACTTTCAGA TGGAGACAGT AAATACTCTC AAACCAGTCA CAAACTAGTG CAGCTTTTGA 
     2941 CAACAACTGC CGAACAGCAG TTACGGCATG CTGATATAGA CACAAGCTGC AAAGATGTCC 
     3001 TGTCTTGCAC AGGCACTTCC AACTCTGCCT CTGCTAACTC TTCAGGAGGT TCTTGTCCCT 
     3061 CTTCTCATAG CTCATTGACA GAACGGCATA AAATTCTACA CCGGCTCTTA CAGGAGGGTA 
     3121 GCCCCTCAGA TATCACCACT TTGTCTGTCG AGCCTGATAA AAAGGACAGT GCATCTACTT 
     3181 CTGTGTCAGT GACTGGACAG GTACAAGGAA ACTCCAGTAT AAAACTAGAA CTGGATGCTT 
     3241 CAAAGAAAAA AGAATCAAAA GACCATCAGC TCCTACGCTA TCTTTTAGAT AAAGATGAGA 
     3301 AAGATTTAAG ATCAACTCCA AACCTGAGCC TGGATGATGT AAAGGTGAAA GTGGAAAAGA 
     3361 AAGAACAGAT GGATCCATGT AATACAAACC CAACCCCAAT GACCAAACCC ACTCCTGAGG 
     3421 AAATAAAACT GGAGGCCCAG AGCCAGTTTA CAGCTGACCT TGACCAGTTT GATCAGTTAC 
     3481 TGCCCACGCT GGAGAAGGCA GCACAGTTGC CAGGCTTATG TGAGACAGAC AGGATGGATG 
     3541 GTGCGGTCAC CAGTGTAACC ATCAAATCGG AGATCCTGCC AGCTTCACTT CAGTCCGCCA 
     3601 CTGCCAGACC CACTTCCAGG CTAAATAGAT TACCTGAGCT GGAATTGGAA GCAATTGATA 
     3661 ACCAATTTGG ACAACCAGGA ACAGGCGATC AGATTCCATG GACAAATAAT ACAGTGACAG 
     3721 CTATAAATCA GAGTAAATCA GAAGACCAGT GTATTAGCTC ACAATTAGAT GAGCTTCTCT 
     3781 GTCCACCCAC AACAGTAGAA GGGAGAAATG ATGAGAAGGC TCTTCTTGAA CAGCTGGTAT 
     3841 CCTTCCTTAG TGGCAAAGAT GAAACTGAGC TAGCTGAACT AGACAGAGCT CTGGGAATTG 
     3901 ACAAACTTGT TCAGGGGGGT GGATTAGATG TATTATCAGA GAGATTTCCA CCACAACAAG 
     3961 CAACGCCACC TTTGATCATG GAAGAAAGAC CCAACCTTTA TTCCCAGCCT TACTCTTCTC 
     4021 CTTCTCCTAC TGCCAATCTC CCTAGCCCTT TCCAAGGCAT GGTCAGGCAA AAACCTTCAC 
     4081 TGGGGACGAT GCCTGTTCAA GTAACACCTC CCCGAGGTGC TTTTTCACCT GGCATGGGCA 
     4141 TGCAGCCCAG GCAAACTCTA AACAGACCTC CGGCTGCACC TAACCAGCTT CGACTTCAAC 
     4201 TACAGCAGCG ATTACAGGGA CAACAGCAGT TGATACACCA AAATCGGCAA GCTATCTTAA 
     4261 ACCAGTTTGC AGCAACTGCT CCTGTTGGCA TCAATATGAG ATCAGGCATG CAACAGCAAA 
     4321 TTACACCTCA GCCACCCCTG AATGCTCAAA TGTTGGCACA ACGTCAGCGG GAACTGTACA 
     4381 GTCAACAGCA CCGACAGAGG CAGCTAATAC AGCAGCAAAG AGCCATGCTT ATGAGGCAGC 
     4441 AAAGCTTTGG GAACAACCTC CCTCCCTCAT CTGGACTACC AGTTCAAATG GGGAACCCCC 
     4501 GTCTTCCTCA GGGTGCTCCA CAGCAATTCC CCTATCCACC AAACTATGGT ACAAATCCAG 
     4561 GAACCCCACC TGCTTCTACC AGCCCGTTTT CACAACTAGC AGCAAATCCT GAAGCATCCT 
     4621 TGGCCAACCG CAACAGCATG GTGAGCAGAG GCATGACAGG AAACATAGGA GGACAGTTTG 
     4681 GCACTGGAAT CAATCCTCAG ATGCAGCAGA ATGTCTTCCA GTATCCAGGA GCAGGAATGG 
     4741 TTCCCCAAGG TGAGGCCAAC TTTGCTCCAT CTCTAAGCCC TGGGAGCTCC ATGGTGCCGA 
     4801 TGCCAATCCC TCCTCCTCAG AGTTCTCTTC TCCAGCAAAC TCCACCTGCC TCCGGGTATC 
     4861 AGTCACCAGA CATGAAGGCC TGGCAGCAAG GAGCGATAGG AAACAACAAT GTGTTCAGTC 
     4921 AAGCTGTCCA GAACCAGCCC ACGCCTGCAC AGCCAGGAGT ATACAACAAC ATGAGCATCA 
     4981 CCGTTTCCAT GGCAGGTGGA AATACGAATG TTCAGAACAT GAACCCAATG ATGGCCCAGA 
     5041 TGCAGATGAG CTCTTTGCAG ATGCCAGGAA TGAACACTGT GTGCCCTGAG CAGATAAATG 
     5101 ATCCCGCACT GAGACACACA GGCCTCTACT GCAACCAGCT CTCATCCACT GACCTTCTCA 
     5161 AAACAGAAGC AGATGGAACC CAGGTGCAAC AGGTTCAGGT GTTTGCTGAC GTCCAGTGTA 
     5221 CAGTGAATCT GGTAGGCGGG GACCCTTACC TGAACCAGCC TGGTCCACTG GGAACTCAAA 
     5281 AGCCCACGTC AGGACCACAG ACCCCCCAGG CCCAGCAGAA GAGCCTCCTT CAGCAGCTAC 
     5341 TGACTGAAAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     5401 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     5461 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     5521 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     5581 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     5641 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     5701 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     5761 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     5821 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     5881 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     5941 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     6001 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     6061 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     6121 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     6181 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     6241 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     6301 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     6361 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     6421 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     6481 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     6541 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     6601 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     6661 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     6721 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     6781 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     6841 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     6901 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     6961 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     7021 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     7081 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     7141 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     7201 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     7261 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     7321 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     7381 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     7441 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     7501 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     7561 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     7621 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     7681 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     7741 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     7801 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     7861 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     7921 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     7981 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     8041 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     8101 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     8161 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     8221 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     8281 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     8341 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     8401 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     8461 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     8521 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     8581 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     8641 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     8701 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     8761 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     8821 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     8881 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     8941 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     9001 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     9061 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     9121 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     9181 CAAAAATTTA ACGCGAATTT T
//