LOCUS       RC223964                7728 bp    DNA     circular  SYN 
DEFINITION  ITPKB (Myc-DDK-tagged)-Human inositol-trisphosphate 3-kinase B (ITPKB)
ACCESSION   NM_002221
VERSION     NM_002221.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3883..3974 
                     /label="Myc-DDK"  
     misc_feature    4004..4589 
                     /label="PolyA signal"  
     misc_feature    4061..4080 
                     /label="Restriction Enzyme"  
     misc_feature    4865..5537 
                     /label="ColE1"  
     misc_feature    5967..6761 
                     /label="Kan/Neo"  
     misc_feature    6796..7098 
                     /label="SV40 ori"  
     misc_feature    7184..7661 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3858..3865 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3858 
                     /label="ITPKB"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTGTGTAC TGCTATGCGC TCAATAGCCT GGTGATCATG AATAGCGCCA 
     1081 ACGAGATGAA GAGCGGCGGC GGCCCGGGGC CCAGTGGCAG CGAGACGCCC CCGCCCCCGA 
     1141 GGAGGGCAGT GCTGAGCCCC GGCAGCGTTT TCAGCCCCGG GAGAGGCGCC TCTTTCCTCT 
     1201 TCCCCCCAGC CGAGTCGCTG TCCCCCGAGG AGCCCCGGAG CCCCGGGGGC TGGCGGAGCG 
     1261 GCCGGCGCAG GCTGAATAGT AGCAGCGGCA GTGGCAGCGG CAGCAGCGGC AGTAGCGTGA 
     1321 GCAGCCCAAG TTGGGCTGGT CGCCTGCGAG GGGACCGGCA GCAGGTGGTG GCAGCCGGTA 
     1381 CCCTCTCCCC GCCAGGGCCG GAGGAGGCCA AGAGGAAGCT GCGGATCTTG CAGCGCGAGT 
     1441 TGCAGAACGT GCAGGTGAAC CAGAAAGTGG GCATGTTTGA GGCGCACATC CAGGCACAGA 
     1501 GCTCCGCCAT TCAAGCGCCC CGCAGCCCGC GTTTGGGCAG GGCTCGCTCG CCCTCCCCGT 
     1561 GCCCCTTCCG CAGCAGCAGT CAGCCCCCTG GAAGGGTCCT GGTTCAGGGC GCCCGGAGCG 
     1621 AGGAACGGAG GACAAAGTCC TGGGGGGAGC AATGTCCAGA GACTTCAGGA ACCGACTCCG 
     1681 GGAGGAAAGG AGGGCCCAGC CTATGCTCCT CGCAGGTGAA GAAAGGAATG CCACCTCTTC 
     1741 CCGGCCGGGC TGCCCCTACA GGATCAGAGG CTCAGGGTCC ATCCGCTTTT GTAAGGATGG 
     1801 AGAAGGGTAT CCCTGCCAGT CCCCGCTGTG GCTCACCCAC AGCTATGGAA ATTGACAAAA 
     1861 GGGGCTCTCC TACCCCGGGA ACTCGGAGCT GCCTAGCTCC CTCATTGGGG CTGTTCGGAG 
     1921 CTAGCTTAAC GATGGCCACG GAAGTGGCAG CGAGAGTTAC ATCCACTGGG CCACACCGTC 
     1981 CACAGGATCT TGCCCTCACT GAGCCGTCTG GGAGAGCCCG TGAGCTTGAG GACCTGCAGC 
     2041 CCCCAGAGGC CCTGGTGGAG AGGCAGGGGC AGTTTCTGGG CAGTGAGACA AGCCCAGCCC 
     2101 CAGAAAGGGG CGGGCCCCGC GATGGAGAAC CCCCTGGGAA GATGGGGAAA GGATATCTGC 
     2161 CCTGTGGCAT GCCGGGCTCT GGGGAGCCTG AAGTGGGCAA AAGGCCAGAG GAGACGACTG 
     2221 TGAGCGTGCA AAGCGCAGAG TCCTCTGATT CCCTGAGCTG GTCCAGGCTG CCCAGGGCCC 
     2281 TGGCCTCCGT AGGCCCTGAG GAGGCCCGAA GTGGGGCCCC CGTGGGCGGG GGGCGTTGGC 
     2341 AGCTCTCCGA CAGAGTGGAG GGAGGGTCCC CAACGCTGGG CTTGCTTGGG GGCAGCCCCT 
     2401 CAGCACAGCC GGGGACCGGG AATGTGGAGG CGGGAATTCC TTCTGGCAGA ATGCTGGAGC 
     2461 CTTTGCCCTG TTGGGACGCT GCGAAAGATC TGAAAGAACC TCAGTGCCCT CCTGGGGACA 
     2521 GGGTGGGTGT GCAGCCTGGG AACTCCAGGG TTTGGCAGGG CACCATGGAG AAAGCCGGTT 
     2581 TGGCTTGGAC GCGTGGCACA GGGGTGCAAT CAGAGGGGAC TTGGGAAAGC CAGCGGCAGG 
     2641 ACAGTGATGC CCTCCCAAGT CCGGAGCTGC TACCCCAAGA TCAGGACAAG CCTTTCCTGA 
     2701 GGAAGGCCTG CAGCCCCAGC AACATACCTG CTGTCATCAT TACAGACATG GGCACCCAGG 
     2761 AGGATGGGGC CTTGGAGGAG ACGCAGGGAA GCCCTCGGGG CAACCTGCCC CTGAGGAAAC 
     2821 TGTCCTCTTC CTCGGCCTCC TCCACGGGCT TCTCCTCATC CTACGAAGAC TCAGAGGAGG 
     2881 ACATCTCCAG TGACCCTGAG CGCACCCTGG ACCCCAACTC AGCCTTCCTG CATACCCTGG 
     2941 ACCAGCAGAA ACCTAGAGTG AGCAAATCAT GGAGGAAGAT AAAAAACATG GTGCACTGGT 
     3001 CTCCCTTCGT CATGTCCTTC AAGAAGAAGT ACCCCTGGAT CCAGCTGGCA GGACACGCAG 
     3061 GGAGTTTCAA GGCAGCTGCC AATGGCAGGA TCCTGAAGAA GCACTGTGAG TCAGAGCAGC 
     3121 GCTGCCTGGA CCGGCTGATG GTGGATGTGC TGAGGCCCTT CGTACCTGCC TACCATGGGG 
     3181 ATGTGGTGAA GGACGGGGAG CGCTACAACC AGATGGACGA CCTGCTGGCC GACTTCGACT 
     3241 CGCCCTGTGT GATGGACTGC AAGATGGGAA TCAGGACCTA CCTGGAGGAG GAGCTCACGA 
     3301 AGGCCCGGAA GAAGCCCAGC CTGCGGAAGG ACATGTACCA GAAGATGATC GAGGTGGACC 
     3361 CCGAGGCCCC CACCGAGGAG GAAAAAGCAC AGCGGGCTGT GACCAAGCCA CGGTACATGC 
     3421 AGTGGCGGGA GACCATCAGC TCCACGGCCA CCCTGGGGTT CAGGATCGAG GGAATCAAGA 
     3481 AAGAAGACGG CACCGTGAAC CGGGACTTCA AGAAGACCAA AACGAGGGAG CAGGTCACCG 
     3541 AGGCCTTCAG AGAGTTCACT AAAGGAAACC ATAACATCCT GATCGCCTAT CGGGACCGGC 
     3601 TGAAGGCCAT TCGAACCACT CTAGAAGTTT CTCCCTTCTT CAAGTGCCAC GAGGTCATTG 
     3661 GCAGCTCCCT CCTCTTCATC CACGACAAGA AGGAACAGGC CAAAGTGTGG ATGATCGACT 
     3721 TTGGGAAAAC CACGCCCCTG CCTGAGGGCC AGACCCTGCA GCATGACGTC CCCTGGCAGG 
     3781 AGGGGAACCG GGAGGATGGC TACCTCTCGG GGCTCAATAA CCTCGTCGAC ATCCTGACCG 
     3841 AGATGTCCCA GGATGCCCCA CTCGCCAGCG GACCGACGCG TACGCGGCCG CTCGAGCAGA 
     3901 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     3961 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     4021 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     4081 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     4141 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     4201 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     4261 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     4321 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     4381 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     4441 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     4501 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     4561 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     4621 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     4681 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     4741 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     4801 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     4861 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     4921 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     4981 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     5041 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     5101 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     5161 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     5221 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     5281 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     5341 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     5401 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     5461 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     5521 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     5581 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     5641 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     5701 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     5761 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     5821 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     5881 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     5941 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     6001 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     6061 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     6121 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     6181 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     6241 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     6301 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     6361 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     6421 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     6481 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     6541 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     6601 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     6661 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     6721 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     6781 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     6841 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     6901 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     6961 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7021 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     7081 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     7141 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     7201 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     7261 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     7321 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     7381 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     7441 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     7501 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     7561 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     7621 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     7681 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//