LOCUS       RC223881                7092 bp    DNA     circular  SYN 
DEFINITION  ADD1 (Myc-DDK-tagged)-Human adducin 1 (alpha) (ADD1), transcript variant 1
ACCESSION   NM_001119
VERSION     NM_001119.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3249..3340 
                     /label="Myc-DDK"  
     misc_feature    3370..3955 
                     /label="PolyA signal"  
     misc_feature    3427..3446 
                     /label="Restriction Enzyme"  
     misc_feature    4231..4903 
                     /label="ColE1"  
     misc_feature    5333..6127 
                     /label="Kan/Neo"  
     misc_feature    6162..6464 
                     /label="SV40 ori"  
     misc_feature    6550..7027 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3231..3237 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3231 
                     /label="ADD1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAATGGTGAT TCTCGTGCTG CGGTGGTGAC CTCACCACCC CCGACCACAG 
     1081 CCCCTCACAA GGAGAGGTAC TTCGACCGAG TAGATGAGAA CAACCCAGAG TACTTGAGGG 
     1141 AGAGGAACAT GGCACCAGAC CTTCGCCAGG ACTTCAACAT GATGGAGCAA AAGAAGAGGG 
     1201 TGTCCATGAT TCTGCAAAGC CCTGCTTTCT GTGAAGAATT GGAATCAATG ATACAGGAGC 
     1261 AATTTAAGAA GGGGAAGAAC CCCACAGGCC TATTGGCATT ACAGCAGATT GCAGATTTTA 
     1321 TGACCACGAA TGTACCAAAT GTCTACCCAG CAGCTCCGCA AGGAGGGATG GCTGCCTTAA 
     1381 ACATGAGTCT TGGTATGGTG ACTCCTGTGA ACGATCTTAG AGGATCTGAT TCTATTGCGT 
     1441 ATGACAAAGG AGAGAAGTTA TTACGGTGTA AATTGGCAGC GTTTTATAGA CTAGCAGATC 
     1501 TCTTTGGGTG GTCTCAGCTT ATCTACAATC ATATCACAAC CAGAGTGAAC TCCGAGCAGG 
     1561 AACACTTCCT CATTGTCCCT TTTGGGCTTC TTTACAGTGA AGTGACTGCA TCCAGTTTGG 
     1621 TTAAGATCAA TCTACAAGGA GATATAGTAG ATCGTGGAAG CACTAATCTG GGAGTGAATC 
     1681 AGGCCGGCTT CACCTTACAC TCTGCAATTT ATGCTGCACG CCCGGACGTG AAGTGCGTCG 
     1741 TGCACATTCA CACCCCAGCA GGGGCTGCGG TCTCTGCAAT GAAATGTGGC CTCTTGCCAA 
     1801 TCTCCCCGGA GGCGCTTTCC CTTGGAGAAG TGGCTTATCA TGACTACCAT GGCATTCTGG 
     1861 TTGATGAAGA GGAAAAAGTT TTGATTCAGA AAAATCTGGG GCCTAAAAGC AAGGTTCTTA 
     1921 TTCTCCGGAA CCATGGGCTC GTGTCAGTTG GAGAGAGCGT TGAGGAGGCC TTCTATTACA 
     1981 TCCATAACCT TGTGGTTGCC TGTGAGATCC AGGTTCGAAC TCTGGCCAGT GCAGGAGGAC 
     2041 CAGACAACTT AGTCCTGCTG AATCCTGAGA AGTACAAAGC CAAGTCCCGT TCCCCAGGGT 
     2101 CTCCGGTAGG GGAAGGCACT GGATCGCCTC CCAAGTGGCA GATTGGTGAG CAGGAATTTG 
     2161 AAGCCCTCAT GCGGATGCTC GATAATCTGG GCTACAGAAC TGGCTACCCT TATCGATACC 
     2221 CTGCTCTGAG AGAGAAGTCT AAAAAATACA GCGATGTGGA GGTTCCTGCT AGTGTCACAG 
     2281 GTTACTCCTT TGCTAGTGAC GGTGATTCGG GCACTTGCTC CCCACTCAGA CACAGTTTTC 
     2341 AGAAGCAGCA GCGGGAGAAG ACAAGATGGC TGAACTCTGG CCGGGGCGAC GAAGCTTCCG 
     2401 AGGAAGGGCA GAATGGAAGC AGTCCCAAGT CGAAGACTAA GTGGACTAAA GAGGATGGAC 
     2461 ATAGAACTTC CACCTCTGCT GTCCCTAACC TGTTTGTTCC ATTGAACACT AACCCAAAAG 
     2521 AGGTCCAGGA GATGAGGAAC AAGATCCGAG AGCAGAATTT ACAGGACATT AAGACGGCTG 
     2581 GCCCTCAGTC CCAGGTTTTG TGTGGTGTAG TGATGGACAG GAGCCTCGTC CAGGGAGAGC 
     2641 TGGTGACGGC CTCCAAGGCC ATCATTGAAA AGGAGTACCA GCCCCACGTC ATTGTGAGCA 
     2701 CCACGGGCCC CAACCCCTTC ACCACACTCA CAGACCGTGA GCTGGAGGAG TACCGCAGGG 
     2761 AGGTGGAGAG GAAGCAGAAG GGCTCTGAAG AGAATCTGGA CGAGGCTAGA GAACAGAAAG 
     2821 AAAAGAGTCC TCCAGACCAG CCTGCGGTCC CCCACCCGCC TCCCAGCACT CCCATCAAGC 
     2881 TGGAGGAAGA CCTTGTGCCG GAGCCGACTA CTGGAGATGA CAGTGATGCT GCCACCTTTA 
     2941 AGCCAACTCT CCCCGATCTG TCCCCTGATG AACCTTCAGA AGCACTCGGC TTCCCAATGT 
     3001 TAGAGAAGGA GGAGGAAGCC CATAGACCCC CAAGCCCCAC TGAGGCCCCT ACTGAGGCCA 
     3061 GCCCCGAGCC AGCCCCAGAC CCAGCCCCGG TGGCTGAAGA GGCTGCCCCC TCAGCTGTCG 
     3121 AGGAGGGGGC CGCCGCGGAC CCTGGCAGCG ATGGGTCTCC AGGCAAGTCC CCGTCCAAAA 
     3181 AGAAGAAGAA GTTCCGTACC CCGTCCTTTC TGAAGAAGAG CAAGAAGAAG AGTGACTCCA 
     3241 CGCGTACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA 
     3301 TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     3361 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     3421 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     3481 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     3541 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     3601 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     3661 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     3721 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     3781 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     3841 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     3901 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     3961 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCGGCGAGC 
     4021 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 
     4081 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 
     4141 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 
     4201 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 
     4261 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 
     4321 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 
     4381 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 
     4441 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 
     4501 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 
     4561 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 
     4621 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 
     4681 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 
     4741 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 
     4801 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 
     4861 TTTTAAATCA ATCTAAAGTA TATATGAGTA ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC 
     4921 GACGTTGGCT GCGAGCCCTG GGCCTTCACC CGAACTTGGG GGGTGGGGTG GGGAAAAGGA 
     4981 AGAAACGCGG GCGTATTGGC CCCAATGGGG TCTCGGTGGG GTATCGACAG AGTGCCAGCC 
     5041 CTGGGACCGA ACCCCGCGTT TATGAACAAA CGACCCAACA CCGTGCGTTT TATTCTGTCT 
     5101 TTTTATTGCC GTCATAGCGC GGGTTCCTTC CGGTATTGTC TCCTTCCGTG TTTCAGTTAG 
     5161 CCTCCCCCTA GGGTGGGCGA AGAACTCCAG CATGAGATCC CCGCGCTGGA GGATCATCCA 
     5221 GCCGGCGTCC CGGAAAACGA TTCCGAAGCC CAACCTTTCA TAGAAGGCGG CGGTGGAATC 
     5281 GAAATCTCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC ATTTCGAACC CCAGAGTCCC 
     5341 GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC GGGAGCGGCG 
     5401 ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC AGCAATATCA 
     5461 CGGGTAGCCA ACGCTATGTC CTGATAGCGA TCCGCCACAC CCAGCCGGCC ACAGTCGATG 
     5521 AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC GCCATGGGTC 
     5581 ACGACGAGAT CCTCGCCGTC GGGCATGCTC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC 
     5641 GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA 
     5701 GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA 
     5761 AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG 
     5821 TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT 
     5881 TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC 
     5941 CGCGCTGCCT CGTCTTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT GACAAAAAGA 
     6001 ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC GATTGTCTGT 
     6061 TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC TGCGTGCAAT 
     6121 CCATCTTGTT CAATCATGCG AAACGATCCT CATCCTGTCT CTTGATCGAT CTTTGCAAAA 
     6181 GCCTAGGCCT CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC 
     6241 CTCGGCCTCT GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGAGAAT GGGCGGAACT 
     6301 GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG ACTATGGTTG CTGACTAATT 
     6361 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCTGGT 
     6421 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     6481 TCCACACCCT AACTGACACA CATTCCACAG CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT 
     6541 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 
     6601 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 
     6661 GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC 
     6721 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC 
     6781 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT 
     6841 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG 
     6901 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT 
     6961 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA 
     7021 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT 
     7081 AACGCGAATT TT
//