LOCUS       RC223833                8085 bp    DNA     circular  SYN 
DEFINITION  UBE3B (Myc-DDK-tagged)-Human ubiquitin protein ligase E3B (UBE3B), transcript variant 3
ACCESSION   NM_183415
VERSION     NM_183415.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4242..4333 
                     /label="Myc-DDK"  
     misc_feature    4363..4948 
                     /label="PolyA signal"  
     misc_feature    4420..4439 
                     /label="Restriction Enzyme"  
     misc_feature    5224..5896 
                     /label="ColE1"  
     misc_feature    6326..7120 
                     /label="Kan/Neo"  
     misc_feature    7155..7457 
                     /label="SV40 ori"  
     misc_feature    7543..8020 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4224..4230 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4224 
                     /label="UBE3B"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTTCACCCTG TCTCAGACCT CGAGAGCATG GTTCATCGAT AGAGCCCGTC 
     1081 AGGCACGAGA AGAAAGGCTT GTGCAGAAGG AACGGGAGCG GGCAGCTGTT GTGATCCAGG 
     1141 CCCATGTCCG GAGTTTTCTC TGTCGGAGTC GACTGCAGAG AGATATCAGG AGAGAGATTG 
     1201 ATGACTTTTT TAAAGCAGAT GACCCTGAGT CCACTAAAAG AAGTGCACTT TGTATTTTCA 
     1261 AGATTGCCAG GAAACTGCTG TTCCTATTCA GAATCAAAGA GGATAATGAG AGATTTGAGA 
     1321 AGTTGTGTCG CAGCATCCTG AGCAGCATGG ATGCTGAGAA TGAGCCTAAG GTGTGGTATG 
     1381 TGTCCCTGGC TTGTTCTAAG GACCTCACCC TCCTTTGGAT TCAACAGATC AAGAACATTT 
     1441 TGTGGTACTG CTGTGATTTT CTCAAGCAGC TCAAGCCTGA AATCCTGCAG GACTCCCGAC 
     1501 TCATCACCCT GTACCTCACG ATGCTTGTCA CCTTCACAGA CACTTCAACG TGGAAAATTC 
     1561 TTCGGGGAAA AGGTGAAAGT CTTCGACCAG CGATGAACCA CATTTGTGCA AATATAATGG 
     1621 GACATCTCAA CCAGCATGGA TTTTATTCTG TGCTGCAGAT ATTGTTAACC CGTGGCCTGG 
     1681 CAAGACCCCG TCCTTGTCTA TCCAAAGGCA CTTTAACAGC AGCTTTTTCT CTAGCGTTAC 
     1741 GCCCTGTGAT TGCTGCACAG TTCTCAGACA ATCTGATTCG GCCGTTCCTC ATCCACATCA 
     1801 TGTCTGTGCC TGCTCTGGTG ACTCATCTCA GCACAGTGAC CCCTGAGCGC CTCACTGTTT 
     1861 TAGAATCCCA TGACATGCTT CGTAAATTCA TCATATTTTT AAGAGACCAA GATCGATGCC 
     1921 GTGATGTATG TGAAAGTTTA GAAGGATGCC ATACGCTTTG TCTAATGGGC AACCTCCTAC 
     1981 ACTTGGGCTC CCTCAGCCCC AGAGTGTTAG AGGAGGAGAC AGATGGGTTC GTGAGTTTGC 
     2041 TCACCCAGAC GCTGTGCTAC TGTCGGAAGT ATGTGTCTCA GAAGAAGTCC AACCTGACCC 
     2101 ACTGGCATCC TGTCCTTGGC TGGTTCTCCC AATCTGTGGA TTATGGCCTT AACGAGTCAA 
     2161 TGCACTTGAT CACCAAACAG CTGCAGTTCT TGTGGGGGGT GCCTCTGATC CGGATCTTCT 
     2221 TCTGTGACAT CCTGAGCAAG AAGCTACTGG AGAGCCAGGA GCCAGCCCAC GCACAGCCAG 
     2281 CATCCCCTCA GAATGTGCTC CCAGTGAAGA GTCTCCTAAA GCGTGCTTTT CAAAAGTCGG 
     2341 CATCAGTCCG GAATATTCTC AGGCCTGTCG GGGGTAAACG GGTCGACTCT GCAGAAGTCC 
     2401 AGAAGGTTTG CAACATCTGT GTCCTCTACC AGACCTCGCT GACAACTCTC ACACAGATTC 
     2461 GGCTGCAGAT ACTCACAGGT CTCACTTACC TTGATGACCT GCTTCCCAAA CTGTGGGCAT 
     2521 TTATCTGTGA GCTCGGGCCC CACGGAGGGT TAAAGCTCTT CTTGGAATGC CTGAACAATG 
     2581 ACACTGAAGA GTCCAAGCAA CTCTTGGCCA TGCTGATGCT GTTCTGTGAC TGTTCGCGGC 
     2641 ACCTCATCAC AATCCTTGAT GACATTGAAG TTTATGAAGA ACAGATTTCA TTCAAACTGG 
     2701 AAGAGCTGGT CACTATCTCC TCTTTCCTGA ATTCTTTTGT GTTTAAGATG ATCTGGGATG 
     2761 GAATTGTAGA GAACGCCAAG GGTGAGACCT TGGAGCTGTT CCAGTCTGTC CACGGGTGGC 
     2821 TTATGGTGCT GTACGAGCGG GACTGCCGGC GGCGCTTCAC CCCCGAGGAC CACTGGCTGC 
     2881 GAAAGGATCT CAAACCTAGC GTGCTCTTCC AAGAACTCGA CAGGGACAGA AAACGGGCAC 
     2941 AGTTGATCCT GCAGTACATC CCACATGTCA TCCCTCACAA AAACAGAGTT CTACTGTTTC 
     3001 GAACCATGGT TACCAAGGAG AAGGAGAAAC TGGGGCTGGT GGAAACCAGC TCTGCCTCCC 
     3061 CGCATGTCAC TCACATCACC ATCCGCCGGT CCAGGATGCT GGAGGACGGC TACGAGCAGC 
     3121 TTAGGCAGCT CTCCCAGCAC GCCATGAAGG GGGTCATCCG TGTGAAGTTT GTCAATGACC 
     3181 TCGGGGTGGA CGAAGCAGGG ATTGATCAAG ACGGTGTTTT TAAGGAGTTC TTGGAAGAGA 
     3241 TCATCAAGAG AGTTTTTGAC CCAGCACTCA ATCTGTTCAA GACAACCAGT GGGGATGAGA 
     3301 GGCTGTACCC CTCACCCACA TCCTACATCC ATGAGAATTA CCTGCAGCTC TTCGAGTTTG 
     3361 TGGGGAAGAT GCTGGGGAAG GCTGTGTATG AGGGAATTGT GGTGGACGTG CCATTTGCAT 
     3421 CCTTCTTCCT GAGCCAACTG CTTGGGCACC ACCACAGCGT CTTCTATAGC TCGGTGGATG 
     3481 AACTGCCTTC TCTGGACTCC GAGTTCTATA AAAACCTCAC CTCCATCAAG CGCTATGATG 
     3541 GGGACATCAC TGACCTGGGC CTGACGCTGT CTTACGACGA GGACGTCATG GGTCAGCTTG 
     3601 TTTGCCATGA ACTGATTCCT GGAGGGAAGA CCATTCCTGT TACAAATGAA AATAAAATTA 
     3661 GCTACATCCA TCTGATGGCA CATTTTCGAA TGCACACTCA AATAAAAAAC CAAACAGCTG 
     3721 CCCTCATTAG CGGATTCCGT TCCATTATCA AACCCGAGTG GATCCGAATG TTCTCAACTC 
     3781 CTGAACTGCA GCGTCTCATC TCTGGCGACA ATGCTGAGAT TGATCTGGAA GATTTAAAGA 
     3841 AGCACACAGT CTACTACGGT GGTTTCCATG GAAGTCACAG AGTCATCATC TGGCTCTGGG 
     3901 ATATTCTGGC CTCCGACTTC ACACCGGATG AGAGAGCTAT GTTTCTGAAG TTCGTGACCA 
     3961 GCTGCTCCAG ACCCCCGCTC CTGGGATTCG CCTACCTCAA GCCTCCATTC TCCATCCGCT 
     4021 GCGTGGAGGT GTCGGACGAT CAGGACACCG GGGACACTCT GGGCAGCGTC CTCCGGGGCT 
     4081 TCTTCACCAT CCGCAAGCGG GAGCCAGGCG GCCGCCTGCC CACCTCCTCC ACCTGCTTCA 
     4141 ACCTGCTCAA GCTGCCCAAC TACAGCAAGA AGAGCGTCCT CCGCGAGAAG CTGCGCTACG 
     4201 CCATCAGCAT GAACACGGGC TTTGAACTCT CCACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     4261 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     4321 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     4381 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     4441 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     4501 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     4561 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     4621 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     4681 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     4741 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     4801 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     4861 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     4921 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     4981 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     5041 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     5101 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     5161 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     5221 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     5281 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     5341 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     5401 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     5461 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     5521 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     5581 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     5641 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     5701 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     5761 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     5821 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     5881 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     5941 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     6001 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     6061 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     6121 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     6181 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     6241 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     6301 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     6361 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     6421 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     6481 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     6541 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     6601 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     6661 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     6721 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     6781 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     6841 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     6901 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     6961 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     7021 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     7081 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     7141 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     7201 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     7261 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     7321 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7381 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7441 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     7501 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     7561 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     7621 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     7681 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     7741 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     7801 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     7861 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     7921 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     7981 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     8041 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
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