LOCUS       RC223816                8376 bp    DNA     circular  SYN 
DEFINITION  LEPR (Myc-DDK-tagged)-Human leptin receptor (LEPR), transcript variant 1
ACCESSION   NM_002303
VERSION     NM_002303.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4533..4624 
                     /label="Myc-DDK"  
     misc_feature    4654..5239 
                     /label="PolyA signal"  
     misc_feature    4711..4730 
                     /label="Restriction Enzyme"  
     misc_feature    5515..6187 
                     /label="ColE1"  
     misc_feature    6617..7411 
                     /label="Kan/Neo"  
     misc_feature    7446..7748 
                     /label="SV40 ori"  
     misc_feature    7834..8311 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4515..4521 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4515 
                     /label="LEPR"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATTTGTCAA AAATTCTGTG TGGTTTTGTT ACATTGGGAA TTTATTTATG 
     1081 TGATAACTGC GTTTAACTTG TCATATCCAA TTACTCCTTG GAGATTTAAG TTGTCTTGCA 
     1141 TGCCACCAAA TTCAACCTAT GACTACTTCC TTTTGCCTGC TGGACTCTCA AAGAATACTT 
     1201 CAAATTCGAA TGGACATTAT GAGACAGCTG TTGAACCTAA GTTTAATTCA AGTGGTACTC 
     1261 ACTTTTCTAA CTTATCCAAA ACAACTTTCC ACTGTTGCTT TCGGAGTGAG CAAGATAGAA 
     1321 ACTGCTCCTT ATGTGCAGAC AACATTGAAG GAAGGACATT TGTTTCAACA GTAAATTCTT 
     1381 TAGTTTTTCA ACAAATAGAT GCAAACTGGA ACATACAGTG CTGGCTAAAA GGAGACTTAA 
     1441 AATTATTCAT CTGTTATGTG GAGTCATTAT TTAAGAATCT ATTCAGGAAT TATAACTATA 
     1501 AGGTCCATCT TTTATATGTT CTGCCTGAAG TGTTAGAAGA TTCACCTCTG GTTCCCCAAA 
     1561 AAGGCAGTTT TCAGATGGTT CACTGCAATT GCAGTGTTCA TGAATGTTGT GAATGTCTTG 
     1621 TGCCTGTGCC AACAGCCAAA CTCAACGACA CTCTCCTTAT GTGTTTGAAA ATCACATCTG 
     1681 GTGGAGTAAT TTTCCGGTCA CCTCTAATGT CAGTTCAGCC CATAAATATG GTGAAGCCTG 
     1741 ATCCACCATT AGGTTTGCAT ATGGAAATCA CAGATGATGG TAATTTAAAG ATTTCTTGGT 
     1801 CCAGCCCACC ATTGGTACCA TTTCCACTTC AATATCAAGT GAAATATTCA GAGAATTCTA 
     1861 CAACAGTTAT CAGAGAAGCT GACAAGATTG TCTCAGCTAC ATCCCTGCTA GTAGACAGTA 
     1921 TACTTCCTGG GTCTTCGTAT GAGGTTCAGG TGAGGGGCAA GAGACTGGAT GGCCCAGGAA 
     1981 TCTGGAGTGA CTGGAGTACT CCTCGTGTCT TTACCACACA AGATGTCATA TACTTTCCAC 
     2041 CTAAAATTCT GACAAGTGTT GGGTCTAATG TTTCTTTTCA CTGCATCTAT AAGAAGGAAA 
     2101 ACAAGATTGT TCCCTCAAAA GAGATTGTTT GGTGGATGAA TTTAGCTGAG AAAATTCCTC 
     2161 AAAGCCAGTA TGATGTTGTG AGTGATCATG TTAGCAAAGT TACTTTTTTC AATCTGAATG 
     2221 AAACCAAACC TCGAGGAAAG TTTACCTATG ATGCAGTGTA CTGCTGCAAT GAACATGAAT 
     2281 GCCATCATCG CTATGCTGAA TTATATGTGA TTGATGTCAA TATCAATATC TCATGTGAAA 
     2341 CTGATGGGTA CTTAACTAAA ATGACTTGCA GATGGTCAAC CAGTACAATC CAGTCACTTG 
     2401 CGGAAAGCAC TTTGCAATTG AGGTATCATA GGAGCAGCCT TTACTGTTCT GATATTCCAT 
     2461 CTATTCATCC CATATCTGAG CCCAAAGATT GCTATTTGCA GAGTGATGGT TTTTATGAAT 
     2521 GCATTTTCCA GCCAATCTTC CTATTATCTG GCTACACAAT GTGGATTAGG ATCAATCACT 
     2581 CTCTAGGTTC ACTTGACTCT CCACCAACAT GTGTCCTTCC TGATTCTGTG GTGAAGCCAC 
     2641 TGCCTCCATC CAGTGTGAAA GCAGAAATTA CTATAAACAT TGGATTATTG AAAATATCTT 
     2701 GGGAAAAGCC AGTCTTTCCA GAGAATAACC TTCAATTCCA GATTCGCTAT GGTTTAAGTG 
     2761 GAAAAGAAGT ACAATGGAAG ATGTATGAGG TTTATGATGC AAAATCAAAA TCTGTCAGTC 
     2821 TCCCAGTTCC AGACTTGTGT GCAGTCTATG CTGTTCAGGT GCGCTGTAAG AGGCTAGATG 
     2881 GACTGGGATA TTGGAGTAAT TGGAGCAATC CAGCCTACAC AGTTGTCATG GATATAAAAG 
     2941 TTCCTATGAG AGGACCTGAA TTTTGGAGAA TAATTAATGG AGATACTATG AAAAAGGAGA 
     3001 AAAATGTCAC TTTACTTTGG AAGCCCCTGA TGAAAAATGA CTCATTGTGC AGTGTTCAGA 
     3061 GATATGTGAT AAACCATCAT ACTTCCTGCA ATGGAACATG GTCAGAAGAT GTGGGAAATC 
     3121 ACACGAAATT CACTTTCCTG TGGACAGAGC AAGCACATAC TGTTACGGTT CTGGCCATCA 
     3181 ATTCAATTGG TGCTTCTGTT GCAAATTTTA ATTTAACCTT TTCATGGCCT ATGAGCAAAG 
     3241 TAAATATCGT GCAGTCACTC AGTGCTTATC CTTTAAACAG CAGTTGTGTG ATTGTTTCCT 
     3301 GGATACTATC ACCCAGTGAT TACAAGCTAA TGTATTTTAT TATTGAGTGG AAAAATCTTA 
     3361 ATGAAGATGG TGAAATAAAA TGGCTTAGAA TCTCTTCATC TGTTAAGAAG TATTATATCC 
     3421 ATGATCATTT TATCCCCATT GAGAAGTACC AGTTCAGTCT TTACCCAATA TTTATGGAAG 
     3481 GAGTGGGAAA ACCAAAGATA ATTAATAGTT TCACTCAAGA TGATATTGAA AAACACCAGA 
     3541 GTGATGCAGG TTTATATGTA ATTGTGCCAG TAATTATTTC CTCTTCCATC TTATTGCTTG 
     3601 GAACATTATT AATATCACAC CAAAGAATGA AAAAGCTATT TTGGGAAGAT GTTCCGAACC 
     3661 CCAAGAATTG TTCCTGGGCA CAAGGACTTA ATTTTCAGAA GCCAGAAACG TTTGAGCATC 
     3721 TTTTTATCAA GCATACAGCA TCAGTGACAT GTGGTCCTCT TCTTTTGGAG CCTGAAACAA 
     3781 TTTCAGAAGA TATCAGTGTT GATACATCAT GGAAAAATAA AGATGAGATG ATGCCAACAA 
     3841 CTGTGGTCTC TCTACTTTCA ACAACAGATC TTGAAAAGGG TTCTGTTTGT ATTAGTGACC 
     3901 AGTTCAACAG TGTTAACTTC TCTGAGGCTG AGGGTACTGA GGTAACCTAT GAGGACGAAA 
     3961 GCCAGAGACA ACCCTTTGTT AAATACGCCA CGCTGATCAG CAACTCTAAA CCAAGTGAAA 
     4021 CTGGTGAAGA ACAAGGGCTT ATAAATAGTT CAGTCACCAA GTGCTTCTCT AGCAAAAATT 
     4081 CTCCATTGAA GGATTCTTTC TCTAATAGCT CATGGGAGAT AGAGGCCCAG GCATTTTTTA 
     4141 TATTATCAGA TCAGCATCCC AACATAATTT CACCACACCT CACATTCTCA GAAGGATTGG 
     4201 ATGAACTTTT GAAATTGGAG GGAAATTTCC CTGAAGAAAA TAATGATAAA AAGTCTATCT 
     4261 ATTATTTAGG GGTCACCTCA ATCAAAAAGA GAGAGAGTGG TGTGCTTTTG ACTGACAAGT 
     4321 CAAGGGTATC GTGCCCATTC CCAGCCCCCT GTTTATTCAC GGACATCAGA GTTCTCCAGG 
     4381 ACAGTTGCTC ACACTTTGTA GAAAATAATA TCAACTTAGG AACTTCTAGT AAGAAGACTT 
     4441 TTGCATCTTA CATGCCTCAA TTCCAAACTT GTTCTACTCA GACTCATAAG ATCATGGAAA 
     4501 ACAAGATGTG TGACCTAACT GTGACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     4561 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     4621 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     4681 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     4741 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     4801 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     4861 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     4921 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     4981 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     5041 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     5101 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     5161 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     5221 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     5281 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     5341 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     5401 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     5461 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     5521 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     5581 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     5641 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     5701 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     5761 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     5821 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     5881 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     5941 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     6001 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     6061 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     6121 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     6181 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     6241 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     6301 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     6361 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     6421 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     6481 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     6541 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     6601 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     6661 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     6721 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     6781 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     6841 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     6901 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     6961 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     7021 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     7081 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     7141 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     7201 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     7261 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     7321 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     7381 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     7441 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     7501 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     7561 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     7621 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7681 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7741 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     7801 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     7861 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     7921 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     7981 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     8041 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     8101 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     8161 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     8221 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     8281 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     8341 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//