LOCUS       RC223738                7626 bp    DNA     circular  SYN 
DEFINITION  OSBP2 (Myc-DDK-tagged)-Human oxysterol binding protein 2 (OSBP2), transcript variant 1
ACCESSION   NM_030758
VERSION     NM_030758.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3783..3874 
                     /label="Myc-DDK"  
     misc_feature    3904..4489 
                     /label="PolyA signal"  
     misc_feature    3961..3980 
                     /label="Restriction Enzyme"  
     misc_feature    4765..5437 
                     /label="ColE1"  
     misc_feature    5867..6661 
                     /label="Kan/Neo"  
     misc_feature    6696..6998 
                     /label="SV40 ori"  
     misc_feature    7084..7561 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3765..3771 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3765 
                     /label="OSBP2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGGAAAGCG GCGGCTCCGA GCCGAGGCGG CGGCTGTGGC GGCCGCTCCC 
     1081 GCGGGCTCTC GTCGCTGTTC ACGGTTGTCC CCTGCCTGTC GTGCCACACG GCGGCGCCGG 
     1141 GCATGAGCGC TTCCACGTCC GGCTCCGGGC CGGAGCCCAA GCCCCAGCCC CAGCCCGTGC 
     1201 CCGAACCGGA GCGGGGACCG CTGTCAGAAC AGGTGTCGGA GGCAGTTTCG GAGGCAGTGC 
     1261 CAAGATCGGA ACCTGTGTCC GAGACGACGT CTGAGCCGGA GCCAGGGGCT GGGCAGCCAT 
     1321 CGGAACTGCT GCAGGGGTCG CGGCCGGGGT CAGAGTCAAG CTCAGGTGTA GGGGCTGGGC 
     1381 CCTTCACTAA GGCCGCATCG GAGCCGCTCT CCCGGGCGGT GGGGAGCGCG ACCTTTCTCA 
     1441 GACCCGAGTC AGGATCGCTG CCAGCGTTAA AGCCCCTGCC TCTTCTGCGA CCAGGACAGG 
     1501 CGAAGACTCC TCTTGGGGTT CCAATGTCGG GGACTGGCAC GACCTCCAGT GCCCCACTGG 
     1561 CCTTACTGCC TCTGGACAGC TTCGAGGGCT GGCTTCTCAA GTGGACCAAC TATCTGAAGG 
     1621 GCTACCAGCG CCGCTGGTTC GTGCTGGGCA ATGGTTTGCT CTCTTACTAC AGAAATCAGG 
     1681 GTGAAATGGC CCACACGTGC CGTGGAACCA TCAACCTGTC CACCGCGCAC ATTGACACGG 
     1741 AGGACTCTTG TGGTATCTTG CTGACCAGTG GGGCCAGGAG CTACCACCTC AAGGCCAGCT 
     1801 CAGAGGTGGA CCGGCAGCAG TGGATCACCG CCCTGGAGCT GGCCAAGGCC AAGGCTGTCC 
     1861 GCGTGATGAA CACTCATTCA GATGACTCTG GGGACGACGA CGAGGCTACC ACCCCAGCCG 
     1921 ACAAGAGCGA GCTGCACCAC ACCCTGAAGA ATCTTTCCCT GAAGTTAGAT GACCTCAGCA 
     1981 CGTGCAATGA CCTCATCGCC AAGCACGGCG CCGCACTCCA GCGCTCCCTG ACAGAGCTGG 
     2041 ACGGCCTCAA GATCCCATCT GAGAGTGGGG AGAAGCTGAA GGTGGTGAAT GAGCGGGCCA 
     2101 CCCTCTTCCG CATCACATCC AATGCTATGA TCAACGCCTG CAGGGACTTC TTGGAACTAG 
     2161 CAGAGATACA CAGTCGGAAA TGGCAGCGGG CACTGCAGTA TGAGCAGGAG CAGCGCGTGC 
     2221 ACTTGGAGGA AACCATTGAG CAGCTGGCGA AGCAGCACAA CAGCCTCGAG CGGGCCTTCC 
     2281 ACAGTGCCCC TGGCCGGCCG GCCAACCCCT CCAAGAGCTT CATTGAGGGA AGCCTCTTGA 
     2341 CTCCCAAAGG AGAGGACAGT GAGGAAGATG AAGATACCGA GTACTTTGAT GCCATGGAAG 
     2401 ACTCCACATC CTTCATCACC GTGATCACCG AGGCCAAGGA AGACAGAAAA GCTGAAGGTA 
     2461 GCACCGGGAC AAGTTCCGTG GACTGGAGCT CAGCAGACAA TGTACTAGAT GGTGCCTCGC 
     2521 TCGTGCCCAA GGGTTCATCC AAAGTCAAGA GGCGAGTCCG CATTCCCAAC AAGCCCAACT 
     2581 ACAGCCTTAA CCTCTGGAGC ATCATGAAGA ACTGCATCGG CCGGGAGCTC TCCAGGATCC 
     2641 CCATGCCGGT GAACTTCAAT GAGCCCCTGT CCATGCTCCA GCGGCTGACA GAGGACCTGG 
     2701 AGTACCACCA CCTGCTGGAC AAGGCAGTGC ACTGCACCAG CTCAGTGGAG CAGATGTGCC 
     2761 TGGTGGCCGC CTTCTCTGTG TCCTCCTACT CCACCACAGT GCACCGCATC GCCAAGCCCT 
     2821 TCAACCCCAT GCTGGGGGAG ACCTTCGAGC TGGACCGCCT CGACGACATG GGCCTGCGCT 
     2881 CCCTCTGTGA GCAGGTGAGC CACCACCCCC CCTCAGCTGC GCACTACGTG TTCTCCAAGC 
     2941 ATGGCTGGAG CCTCTGGCAG GAGATCACCA TCTCCAGCAA GTTCCGGGGA AAATACATCT 
     3001 CCATCATGCC GCTAGGTGCC ATCCACTTAG AATTCCAGGC CAGTGGGAAT CACTACGTGT 
     3061 GGAGGAAGAG CACCTCAACT GTTCACAACA TCATCGTGGG CAAGCTCTGG ATCGACCAGT 
     3121 CAGGGGACAT CGAGATTGTG AACCATAAGA CCAATGACCG GTGCCAGCTG AAGTTCCTGC 
     3181 CCTACAGCTA CTTCTCCAAA GAGGCAGCCC GGAAGGTGAC AGGAGTGGTG AGTGACAGCC 
     3241 AGGGCAAGGC CCATTACGTG CTGTCCGGCT CGTGGGATGA ACAAATGGAG TGCTCCAAGG 
     3301 TCATGCATAG CAGTCCCAGC AGCCCCAGCT CTGACGGGAA GCAGAAGACA GTGTACCAGA 
     3361 CCCTGTCAGC CAAGCTGCTG TGGAAGAAGT ACCCGCTGCC GGAGAACGCG GAGAACATGT 
     3421 ACTACTTCTC AGAGCTGGCC CTGACCCTCA ACGAGCACGA GGAGGGCGTA GCGCCAACCG 
     3481 ACAGCCGCCT GCGGCCCGAC CAGCGGCTGA TGGAGAAGGG CCGTTGGGAC GAGGCCAATA 
     3541 CCGAGAAGCA GCGGCTGGAG GAGAAGCAGC GCCTGTCGCG GCGCCGGCGG CTGGAGGCCT 
     3601 GCGGGCCGGG CAGCAGCTGC AGCTCGGAGG AAGAGAAGGA GGCGGATGCC TACACGCCAC 
     3661 TGTGGTTTGA GAAGAGGCTG GATCCGCTGA CCGGGGAGAT GGCCTGTGTG TACAAGGGCG 
     3721 GCTACTGGGA GGCCAAGGAG AAGCAAGACT GGCATATGTG CCCCAACATC TTCACGCGTA 
     3781 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     3841 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     3901 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     3961 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     4021 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     4081 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     4141 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     4201 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     4261 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     4321 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     4381 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     4441 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     4501 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     4561 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     4621 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     4681 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     4741 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     4801 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     4861 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     4921 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     4981 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     5041 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     5101 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     5161 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     5221 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     5281 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     5341 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     5401 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     5461 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     5521 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     5581 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     5641 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     5701 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     5761 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     5821 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     5881 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     5941 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     6001 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     6061 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     6121 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     6181 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     6241 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     6301 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     6361 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     6421 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     6481 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     6541 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     6601 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     6661 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     6721 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     6781 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     6841 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     6901 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     6961 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7021 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     7081 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     7141 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     7201 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     7261 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     7321 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     7381 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     7441 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     7501 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     7561 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     7621 AATTTT
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