LOCUS       RC223731                7722 bp    DNA     circular  SYN 
DEFINITION  BRDT (Myc-DDK-tagged)-Human bromodomain, testis-specific (BRDT), transcript variant 2
ACCESSION   NM_001726
VERSION     NM_001726.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3879..3970 
                     /label="Myc-DDK"  
     misc_feature    4000..4585 
                     /label="PolyA signal"  
     misc_feature    4057..4076 
                     /label="Restriction Enzyme"  
     misc_feature    4861..5533 
                     /label="ColE1"  
     misc_feature    5963..6757 
                     /label="Kan/Neo"  
     misc_feature    6792..7094 
                     /label="SV40 ori"  
     misc_feature    7180..7657 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3861..3867 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3861 
                     /label="BRDT"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCTCTGCCA AGTCGACAAA CAGCTATTAT TGTTAACCCT CCTCCACCAG 
     1081 AATATATAAA TACTAAGAAA AATGGGCGAT TGACAAATCA ACTTCAGTAT CTACAAAAAG 
     1141 TTGTCCTAAA GGATTTATGG AAGCATAGTT TTTCATGGCC CTTTCAACGT CCTGTGGATG 
     1201 CTGTGAAACT ACAGTTGCCT GATTATTATA CCATTATAAA AAACCCAATG GATTTAAATA 
     1261 CAATTAAGAA GCGCTTGGAG AATAAATATT ATGCGAAGGC TTCAGAATGT ATAGAAGACT 
     1321 TCAATACAAT GTTCTCAAAT TGTTATTTAT ATAACAAGCC TGGAGATGAC ATTGTTCTTA 
     1381 TGGCACAAGC TCTAGAGAAG CTGTTTATGC AGAAATTATC TCAGATGCCA CAAGAAGAGC 
     1441 AAGTTGTGGG TGTTAAGGAA AGAATCAAGA AAGGCACTCA ACAGAATATA GCTGTTTCTT 
     1501 CTGCTAAAGA AAAATCATCA CCCAGCGCAA CAGAAAAAGT ATTTAAGCAG CAAGAAATTC 
     1561 CTTCTGTATT TCCTAAGACA TCTATTTCTC CCTTGAACGT GGTACAGGGA GCTTCAGTCA 
     1621 ACTCCAGTTC ACAAACTGCG GCCCAAGTTA CAAAAGGTGT GAAGAGGAAA GCAGATACAA 
     1681 CAACTCCTGC AACTTCAGCA GTTAAAGCAA GTAGTGAATT TTCTCCAACA TTCACAGAAA 
     1741 AATCAGTGGC ACTGCCACCT ATAAAAGAAA ATATGCCAAA GAATGTTTTG CCAGATTCTC 
     1801 AGCAACAATA TAATGTTGTG AAGACTGTTA AAGTAACTGA ACAATTAAGG CACTGTAGTG 
     1861 AGATTCTTAA AGAAATGCTT GCAAAGAAAC ATTTTTCATA TGCATGGCCC TTTTATAATC 
     1921 CTGTTGACGT TAATGCTTTG GGACTCCATA ACTACTATGA CGTTGTCAAA AATCCGATGG 
     1981 ATCTTGGAAC TATTAAGGAG AAAATGGATA ACCAAGAATA TAAGGATGCA TACAAATTTG 
     2041 CGGCAGATGT TAGATTAATG TTCATGAATT GCTACAAGTA CAATCCTCCA GATCACGAAG 
     2101 TTGTGACAAT GGCAAGAATG CTTCAGGATG TTTTCGAAAC GCATTTTTCA AAGATCCCGA 
     2161 TTGAACCTGT TGAGAGTATG CCTTTATGTT ACATCAAAAC AGATATCACA GAAACCACTG 
     2221 GTAGAGAGAA CACTAATGAA GCCTCCTCTG AAGGGAACTC TTCTGATGAT TCTGAAGATG 
     2281 AGCGAGTTAA GCGTCTTGCA AAGCTTCAGG AGCAGCTTAA AGCTGTACAT CAACAGCTCC 
     2341 AGGTTTTGTC CCAAGTACCT TTCCGTAAGC TAAATAAAAA GAAAGAGAAG TCTAAAAAGG 
     2401 AAAAGAAAAA AGAAAAGGTT AATAACAGCA ATGAAAATCC AAGAAAAATG TGTGAGCAAA 
     2461 TGAGGCTAAA GGAAAAGTCC AAGAGAAATC AGCCAAAGAA AAGGAAACAA CAGTTCATTG 
     2521 GTCTAAAATC TGAAGATGAA GATAATGCTA AACCTATGAA CTATGATGAG AAAAGGCAGT 
     2581 TAAGTCTGAA TATAAACAAA CTCCCTGGAG ATAAACTTGG GCGAGTAGTT CACATAATAC 
     2641 AATCAAGAGA GCCTTCTCTG AGCAATTCCA ATCCTGATGA GATAGAGATA GACTTTGAAA 
     2701 CACTGAAAGC ATCAACACTA AGAGAATTAG AAAAATATGT TTCGGCATGT CTAAGAAAGA 
     2761 GACCATTAAA ACCTCCTGCT AAGAAAATAA TGATGTCCAA AGAAGAACTT CACTCACAGA 
     2821 AAAAACAGGA ATTGGAAAAG CGGTTACTGG ATGTTAATAA TCAGTTAAAT TCTAGAAAAC 
     2881 GTCAAACAAA ATCTGATAAA ACGCAACCAT CCAAAGCTGT TGAAAATGTT TCCCGACTGA 
     2941 GTGAGAGCAG CAGCAGCAGC AGCAGCTCAT CAGAGTCTGA AAGTAGCAGC AGTGACTTAA 
     3001 GCTCTTCAGA CAGCAGTGAT TCTGAATCAG AAATGTTCCC TAAGTTTACA GAAGTAAAAC 
     3061 CAAATGATTC TCCTTCTAAA GAGAATGTAA AGAAAATGAA GAATGAATGC ATACTGCCTG 
     3121 AAGGAAGAAC AGGCGTCACA CAGATAGGAT ATTGTGTGCA AGACACAACC TCTGCCAATA 
     3181 CTACCCTTGT TCATCAGACC ACACCTTCAC ATGTAATGCC ACCAAATCAC CACCAATTAG 
     3241 CATTTAATTA TCAAGAATTA GAACATTTAC AGACTGTGAA AAACATTTCA CCTTTACAAA 
     3301 TTCTGCCTCC CTCAGGTGAT TCTGAACAGC TCTCAAATGG CATAACTGTG ATGCATCCAT 
     3361 CTGGTGATAG TGACACAACG ATGTTAGAAT CTGAATGTCA AGCTCCTGTA CAGAAGGATA 
     3421 TAAAGATTAA GAATGCAGAT TCATGGAAAA GTTTAGGCAA ACCAGTGAAA CCATCAGGTG 
     3481 TAATGAAATC CTCAGATGAG CTCTTCAACC AATTTAGAAA AGCAGCCATA GAAAAGGAAG 
     3541 TAAAAGCTCG GACACAGGAA CTCATACGGA AGCATTTGGA ACAAAATACA AAGGAACTAA 
     3601 AAGCATCTCA AGAAAATCAG AGGGATCTTG GGAATGGATT GACTGTAGAA TCTTTTTCAA 
     3661 ATAAAATACA AAACAAGTGC TCTGGAGAAG AGCAGAAAGA ACATCAGCAG TCATCAGAAG 
     3721 CTCAAGATAA ATCCAAACTC TGGCTTCTCA AAGACCGTGA TTTAGCAAGG CAGAAAGAAC 
     3781 AAGAGAGGAG GAGGAGAGAA GCAATGGTGG GTACCATTGA TATGACCCTT CAAAGTGACA 
     3841 TTATGACAAT GTTTGAAAAC AACTTTGATA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     3901 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     3961 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     4021 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     4081 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     4141 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     4201 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     4261 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     4321 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     4381 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     4441 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     4501 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     4561 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     4621 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     4681 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     4741 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     4801 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     4861 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     4921 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     4981 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     5041 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     5101 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     5161 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     5221 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     5281 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     5341 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     5401 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     5461 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     5521 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     5581 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     5641 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     5701 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     5761 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     5821 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     5881 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     5941 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     6001 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     6061 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     6121 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     6181 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     6241 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     6301 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     6361 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     6421 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     6481 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     6541 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     6601 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     6661 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     6721 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     6781 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     6841 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     6901 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     6961 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7021 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7081 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     7141 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     7201 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     7261 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     7321 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     7381 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     7441 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     7501 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     7561 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     7621 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     7681 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//