LOCUS       RC223702                8799 bp    DNA     circular  SYN 
DEFINITION  ACE (Myc-DDK-tagged)-Human angiotensin I converting enzyme (peptidyl-dipeptidase A) 1 (ACE), transcript variant 1
ACCESSION   NM_000789
VERSION     NM_000789.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4956..5047 
                     /label="Myc-DDK"  
     misc_feature    5077..5662 
                     /label="PolyA signal"  
     misc_feature    5134..5153 
                     /label="Restriction Enzyme"  
     misc_feature    5938..6610 
                     /label="ColE1"  
     misc_feature    7040..7834 
                     /label="Kan/Neo"  
     misc_feature    7869..8171 
                     /label="SV40 ori"  
     misc_feature    8257..8734 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4938..4944 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4938 
                     /label="ACE"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGGGCCGCC TCGGGCCGCC GGGGGCCGGG GCTGCTGCTG CCGCTGCCGC 
     1081 TGCTGTTGCT GCTGCCGCCG CAGCCCGCCC TGGCGTTGGA CCCCGGGCTG CAGCCCGGCA 
     1141 ACTTTTCTGC TGACGAGGCC GGGGCGCAGC TCTTCGCGCA GAGCTACAAC TCCAGCGCCG 
     1201 AACAGGTGCT GTTCCAGAGC GTGGCCGCCA GCTGGGCGCA CGACACCAAC ATCACCGCGG 
     1261 AGAATGCAAG GCGCCAGGAG GAAGCAGCCC TGCTCAGCCA GGAGTTTGCG GAGGCCTGGG 
     1321 GCCAGAAGGC CAAGGAGCTG TATGAACCGA TCTGGCAGAA CTTCACGGAC CCGCAGCTGC 
     1381 GCAGGATCAT CGGAGCTGTG CGCACCCTGG GCTCTGCCAA CCTGCCCCTG GCTAAGCGGC 
     1441 AGCAGTACAA CGCCCTGCTA AGCAACATGA GCAGGATCTA CTCCACCGCC AAGGTCTGCC 
     1501 TCCCCAACAA GACTGCCACC TGCTGGTCCC TGGACCCAGA TCTCACCAAC ATCCTGGCTT 
     1561 CCTCGCGAAG CTACGCCATG CTCCTGTTTG CCTGGGAGGG CTGGCACAAC GCTGCGGGCA 
     1621 TCCCGCTGAA ACCGCTGTAC GAGGATTTCA CTGCCCTCAG CAATGAAGCC TACAAGCAGG 
     1681 ACGGCTTCAC AGACACGGGG GCCTACTGGC GCTCCTGGTA CAACTCCCCC ACCTTCGAGG 
     1741 ACGATCTGGA ACACCTCTAC CAACAGCTAG AGCCCCTCTA CCTGAACCTC CATGCCTTCG 
     1801 TCCGCCGCGC ACTGCATCGC CGATACGGAG ACAGATACAT CAACCTCAGG GGACCCATCC 
     1861 CTGCTCATCT GCTGGGAGAC ATGTGGGCCC AGAGCTGGGA AAACATCTAC GACATGGTGG 
     1921 TGCCTTTCCC AGACAAGCCC AACCTCGATG TCACCAGTAC TATGCTGCAG CAGGGCTGGA 
     1981 ACGCCACGCA CATGTTCCGG GTGGCAGAGG AGTTCTTCAC CTCCCTGGAG CTCTCCCCCA 
     2041 TGCCTCCCGA GTTCTGGGAA GGGTCGATGC TGGAGAAGCC GGCCGACGGG CGGGAAGTGG 
     2101 TGTGCCACGC CTCGGCTTGG GACTTCTACA ACAGGAAAGA CTTCAGGATC AAGCAGTGCA 
     2161 CACGGGTCAC GATGGACCAG CTCTCCACAG TGCACCATGA GATGGGCCAT ATACAGTACT 
     2221 ACCTGCAGTA CAAGGATCTG CCCGTCTCCC TGCGTCGGGG GGCCAACCCC GGCTTCCATG 
     2281 AGGCCATTGG GGACGTGCTG GCGCTCTCGG TCTCCACTCC TGAACATCTG CACAAAATCG 
     2341 GCCTGCTGGA CCGTGTCACC AATGACACGG AAAGTGACAT CAATTACTTG CTAAAAATGG 
     2401 CACTGGAAAA AATTGCCTTC CTGCCCTTTG GCTACTTGGT GGACCAGTGG CGCTGGGGGG 
     2461 TCTTTAGTGG GCGTACCCCC CCTTCCCGCT ACAACTTCGA CTGGTGGTAT CTTCGAACCA 
     2521 AGTATCAGGG GATCTGTCCT CCTGTTACCC GAAACGAAAC CCACTTTGAT GCTGGAGCTA 
     2581 AGTTTCATGT TCCAAATGTG ACACCATACA TCAGGTACTT TGTGAGTTTT GTCCTGCAGT 
     2641 TCCAGTTCCA TGAAGCCCTG TGCAAGGAGG CAGGCTATGA GGGCCCACTG CACCAGTGTG 
     2701 ACATCTACCG GTCCACCAAG GCAGGGGCCA AGCTCCGGAA GGTGCTGCAG GCTGGCTCCT 
     2761 CCAGGCCCTG GCAGGAGGTG CTGAAGGACA TGGTCGGCTT AGATGCCCTG GATGCCCAGC 
     2821 CGCTGCTCAA GTACTTCCAG CCAGTCACCC AGTGGCTGCA GGAGCAGAAC CAGCAGAACG 
     2881 GCGAGGTCCT GGGCTGGCCC GAGTACCAGT GGCACCCGCC GTTGCCTGAC AACTACCCGG 
     2941 AGGGCATAGA CCTGGTGACT GATGAGGCTG AGGCCAGCAA GTTTGTGGAG GAATATGACC 
     3001 GGACATCCCA GGTGGTGTGG AACGAGTATG CCGAGGCCAA CTGGAACTAC AACACCAACA 
     3061 TCACCACAGA GACCAGCAAG ATTCTGCTGC AGAAGAACAT GCAAATAGCC AACCACACCC 
     3121 TGAAGTACGG CACCCAGGCC AGGAAGTTTG ATGTGAACCA GTTGCAGAAC ACCACTATCA 
     3181 AGCGGATCAT AAAGAAGGTT CAGGACCTAG AACGGGCAGC ACTGCCTGCC CAGGAGCTGG 
     3241 AGGAGTACAA CAAGATCCTG TTGGATATGG AAACCACCTA CAGCGTGGCC ACTGTGTGCC 
     3301 ACCCGAATGG CAGCTGCCTG CAGCTCGAGC CAGATCTGAC GAATGTGATG GCCACGTCCC 
     3361 GGAAATATGA AGACCTGTTA TGGGCATGGG AGGGCTGGCG AGACAAGGCG GGGAGAGCCA 
     3421 TCCTCCAGTT TTACCCGAAA TACGTGGAAC TCATCAACCA GGCTGCCCGG CTCAATGGCT 
     3481 ATGTAGATGC AGGGGACTCG TGGAGGTCTA TGTACGAGAC ACCATCCCTG GAGCAAGACC 
     3541 TGGAGCGGCT CTTCCAGGAG CTGCAGCCAC TCTACCTCAA CCTGCATGCC TACGTGCGCC 
     3601 GGGCCCTGCA CCGTCACTAC GGGGCCCAGC ACATCAACCT GGAGGGGCCC ATTCCTGCTC 
     3661 ACCTGCTGGG GAACATGTGG GCGCAGACCT GGTCCAACAT CTATGACTTG GTGGTGCCCT 
     3721 TCCCTTCAGC CCCCTCGATG GACACCACAG AGGCTATGCT AAAGCAGGGC TGGACGCCCA 
     3781 GGAGGATGTT TAAGGAGGCT GATGATTTCT TCACCTCCCT GGGGCTGCTG CCCGTGCCTC 
     3841 CTGAGTTCTG GAACAAGTCG ATGCTGGAGA AGCCAACCGA CGGGCGGGAG GTGGTCTGCC 
     3901 ACGCCTCGGC CTGGGACTTC TACAACGGCA AGGACTTCCG GATCAAGCAG TGCACCACCG 
     3961 TGAACTTGGA GGACCTGGTG GTGGCCCACC ACGAAATGGG CCACATCCAG TATTTCATGC 
     4021 AGTACAAAGA CTTACCTGTG GCCTTGAGGG AGGGTGCCAA CCCCGGCTTC CATGAGGCCA 
     4081 TTGGGGACGT GCTAGCCCTC TCAGTGTCTA CGCCCAAGCA CCTGCACAGT CTCAACCTGC 
     4141 TGAGCAGTGA GGGTGGCAGC GACGAGCATG ACATCAACTT TCTGATGAAG ATGGCCCTTG 
     4201 ACAAGATCGC CTTTATCCCC TTCAGCTACC TCGTCGATCA GTGGCGCTGG AGGGTATTTG 
     4261 ATGGAAGCAT CACCAAGGAG AACTATAACC AGGAGTGGTG GAGCCTCAGG CTGAAGTACC 
     4321 AGGGCCTCTG CCCCCCAGTG CCCAGGACTC AAGGTGACTT TGACCCAGGG GCCAAGTTCC 
     4381 ACATTCCTTC TAGCGTGCCT TACATCAGGT ACTTTGTCAG CTTCATCATC CAGTTCCAGT 
     4441 TCCACGAGGC ACTGTGCCAG GCAGCTGGCC ACACGGGCCC CCTGCACAAG TGTGACATCT 
     4501 ACCAGTCCAA GGAGGCCGGG CAGCGCCTGG CGACCGCCAT GAAGCTGGGC TTCAGTAGGC 
     4561 CGTGGCCGGA AGCCATGCAG CTGATCACGG GCCAGCCCAA CATGAGCGCC TCGGCCATGT 
     4621 TGAGCTACTT CAAGCCGCTG CTGGACTGGC TCCGCACGGA GAACGAGCTG CATGGGGAGA 
     4681 AGCTGGGCTG GCCGCAGTAC AACTGGACGC CGAACTCCGC TCGCTCAGAA GGGCCCCTCC 
     4741 CAGACAGCGG CCGCGTCAGC TTCCTGGGCC TGGACCTGGA TGCGCAGCAG GCCCGCGTGG 
     4801 GCCAGTGGCT GCTGCTCTTC CTGGGCATCG CCCTGCTGGT AGCCACCCTG GGCCTCAGCC 
     4861 AGCGGCTCTT CAGCATCCGC CACCGCAGCC TCCACCGGCA CTCCCACGGG CCCCAGTTCG 
     4921 GCTCCGAGGT GGAGCTGAGA CACTCCACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     4981 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     5041 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     5101 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     5161 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     5221 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     5281 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     5341 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     5401 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     5461 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     5521 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     5581 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     5641 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     5701 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     5761 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     5821 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     5881 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     5941 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     6001 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     6061 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     6121 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     6181 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     6241 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     6301 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     6361 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     6421 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     6481 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     6541 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     6601 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     6661 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     6721 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     6781 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     6841 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     6901 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     6961 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     7021 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     7081 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     7141 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     7201 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     7261 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     7321 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     7381 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     7441 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     7501 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     7561 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     7621 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     7681 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     7741 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     7801 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     7861 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     7921 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     7981 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     8041 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     8101 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     8161 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     8221 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     8281 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     8341 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     8401 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     8461 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     8521 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     8581 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     8641 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     8701 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     8761 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//