LOCUS       RC223698                8949 bp    DNA     circular  SYN 
DEFINITION  PARD3 (Myc-DDK-tagged)-Human par-3 partitioning defective 3 homolog (C. elegans) (PARD3), transcript variant 1
ACCESSION   NM_019619
VERSION     NM_019619.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5106..5197 
                     /label="Myc-DDK"  
     misc_feature    5227..5812 
                     /label="PolyA signal"  
     misc_feature    5284..5303 
                     /label="Restriction Enzyme"  
     misc_feature    6088..6760 
                     /label="ColE1"  
     misc_feature    7190..7984 
                     /label="Kan/Neo"  
     misc_feature    8019..8321 
                     /label="SV40 ori"  
     misc_feature    8407..8884 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5088..5094 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5088 
                     /label="PARD3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAAGTGACC GTGTGCTTCG GACGGACCCG GGTGGTCGTG CCGTGCGGGG 
     1081 ACGGCCACAT GAAAGTTTTC AGCCTCATCC AGCAGGCGGT GACCCGCTAC CGGAAGGCCA 
     1141 TCGCCAAGGA TCCAAACTAC TGGATACAGG TGCATCGCTT GGAACATGGA GATGGAGGAA 
     1201 TACTAGACCT TGATGACATT CTTTGTGATG TAGCAGACGA TAAAGACAGA CTGGTAGCAG 
     1261 TGTTTGATGA GCAGGATCCA CATCACGGAG GTGATGGCAC CAGTGCCAGT TCCACGGGTA 
     1321 CCCAGAGCCC AGAGATATTT GGTAGTGAGC TTGGCACCAA CAATGTCTCA GCCTTTCAGC 
     1381 CTTACCAAGC AACAAGTGAA ATTGAGGTCA CACCTTCAGT CCTTCGAGCA AATATGCCTC 
     1441 TTCATGTTCG ACGCAGTAGT GACCCAGCTC TAATTGGCCT CTCCACTTCT GTCAGTGATA 
     1501 GTAATTTTTC CTCTGAAGAG CCTTCAAGGA AAAATCCCAC ACGCTGGTCA ACAACAGCTG 
     1561 GCTTCCTCAA GCAGAACACT GCTGGGAGTC CTAAAACCTG CGACAGGAAG AAAGATGAAA 
     1621 ACTACAGAAG CCTCCCGCGG GATACTAGTA ACTGGTCTAA CCAATTTCAG AGAGACAATG 
     1681 CTCGCTCGTC TCTGAGTGCC AGTCACCCAA TGGTGGGCAA GTGGCTGGAG AAACAAGAAC 
     1741 AGGATGAGGA TGGGACAGAA GAGGATAACA GTCGTGTTGA ACCTGTTGGA CATGCTGACA 
     1801 CGGGTTTGGA GCATATACCC AACTTTTCTC TGGATGATAT GGTAAAGCTC GTAGAAGTCC 
     1861 CCAACGATGG AGGGCCTCTG GGAATCCATG TAGTGCCTTT CAGTGCTCGA GGCGGCAGAA 
     1921 CCCTGGGGTT ATTAGTAAAA CGATTGGAGA AAGGTGGTAA AGCTGAACAT GAAAATCTTT 
     1981 TTCGTGAGAA TGATTGCATT GTCAGGATTA ATGATGGCGA CCTTCGAAAT AGAAGATTTG 
     2041 AACAAGCACA ACATATGTTT CGCCAAGCCA TGCGTACACC CATCATTTGG TTCCATGTGG 
     2101 TTCCTGCAGC AAATAAAGAG CAGTATGAAC AACTATCCCA AAGTGAGAAG AACAATTACT 
     2161 ATTCAAGCCG TTTTAGCCCT GACAGCCAGT ATATTGACAA CAGGAGTGTG AACAGTGCAG 
     2221 GGCTTCACAC GGTGCAGAGA GCACCCCGAC TGAACCACCC GCCTGAGCAG ATAGACTCTC 
     2281 ACTCAAGACT ACCTCATAGC GCACACCCCT CGGGAAAACC ACCATCCGCT CCAGCCTCGG 
     2341 CACCTCAGAA TGTATTTAGT ACGACTGTAA GCAGTGGTTA TAACACCAAA AAAATAGGCA 
     2401 AGAGGCTTAA TATCCAGCTT AAGAAAGGTA CAGAAGGTTT GGGATTCAGC ATCACTTCCA 
     2461 GAGATGTAAC AATAGGTGGC TCAGCTCCAA TCTATGTGAA AAACATTCTC CCCCGGGGGG 
     2521 CGGCCATTCA GGATGGCCGA CTTAAGGCAG GAGACAGACT TATAGAGGTA AATGGAGTAG 
     2581 ATTTAGTGGG CAAATCCCAA GAGGAAGTTG TTTCGCTGTT GAGAAGCACC AAGATGGAAG 
     2641 GAACTGTGAG CCTTCTGGTC TTTCGCCAGG AAGACGCCTT CCACCCAAGG GAACTGAATG 
     2701 CAGAGCCAAG CCAGATGCAG ATTCCAAAAG AAACGAAAGC AGAAGATGAG GATATTGTTC 
     2761 TTACACCTGA TGGCACCAGG GAATTTCTGA CATTTGAAGT CCCACTTAAT GATTCAGGAT 
     2821 CTGCAGGCCT TGGTGTCAGT GTCAAAGGTA ACCGGTCAAA AGAGAACCAC GCAGATTTGG 
     2881 GAATCTTTGT CAAGTCCATT ATTAATGGAG GAGCAGCATC TAAAGATGGA AGGCTTCGGG 
     2941 TGAATGATCA ACTGATAGCA GTAAATGGAG AATCCCTGTT GGGCAAGACA AACCAAGATG 
     3001 CCATGGAAAC CCTAAGAAGG TCTATGTCTA CTGAAGGCAA TAAACGAGGA ATGATCCAGC 
     3061 TTATTGTTGC AAGGAGAATA AGCAAGTGCA ATGAGCTGAA GTCACCTGGG AGCCCCCCTG 
     3121 GACCTGAGCT GCCCATTGAA ACAGCGTTGG ATGATAGAGA ACGAAGAATT TCCCATTCCC 
     3181 TCTACAGTGG GATTGAGGGG CTTGATGAAT CCCCCAGCAG AAATGCTGCC CTCAGTAGGA 
     3241 TAATGGGTGA GTCAGGTAAA TACCAGCTGT CCCCTACAGT GAATATGCCC CAAGATGACA 
     3301 CTGTCATTAT AGAAGATGAC AGGTTGCCAG TGCTTCCTCC ACATCTCTCT GACCAGTCCT 
     3361 CTTCCAGCTC CCATGATGAT GTGGGGTTTG TGACGGCAGA TGCTGGTACT TGGGCCAAGG 
     3421 CTGCAATCAG TGATTCAGCC GACTGCTCTT TGAGTCCAGA TGTTGATCCA GTTCTTGCTT 
     3481 TTCAACGAGA AGGATTTGGA CGTCAGAGTA TGTCAGAAAA ACGCACAAAG CAATTTTCAG 
     3541 ATGCCAGTCA ATTGGATTTC GTTAAAACAC GAAAATCAAA AAGCATGGAT TTAGGTATAG 
     3601 CTGACGAGAC TAAACTCAAT ACAGTGGATG ACCAGAAAGC AGGTTCTCCC AGCAGAGATG 
     3661 TGGGTCCTTC CCTGGGTCTG AAGAAGTCAA GCTCGTTGGA GAGTCTGCAG ACCGCAGTTG 
     3721 CCGAGGTGAC TTTGAATGGG GATATTCCTT TCCATCGTCC ACGGCCGCGG ATAATCAGAG 
     3781 GCAGGGGATG CAATGAGAGC TTCAGAGCTG CCATCGACAA ATCTTATGAT AAACCCGCGG 
     3841 TAGATGATGA TGATGAAGGC ATGGAGACCT TGGAAGAAGA CACAGAAGAA AGTTCAAGAT 
     3901 CAGGGAGAGA GTCTGTATCC ACAGCCAGTG ATCAGCCTTC CCACTCTCTG GAGAGACAAA 
     3961 TGAATGGAAA CCAAGAGAAA GGTGATAAGA CTGATAGAAA AAAGGATAAA ACTGGAAAAG 
     4021 AAAAGAAGAA AGATAGAGAT AAGGAGAAGG ATAAAATGAA AGCCAAGAAG GGAATGCTGA 
     4081 AGGGCTTGGG AGACATGTTC AGGTTTGGCA AACATCGAAA AGATGACAAG ATTGAGAAAA 
     4141 CGGGTAAAAT AAAAATACAG GAATCCTTTA CATCAGAAGA GGAGAGGATA CGAATGAAGC 
     4201 AGGAGCAGGA GAGGATTCAA GCCAAAACTC GAGAATTTAG GGAACGACAA GCTCGAGAGC 
     4261 GTGACTATGC TGAAATTCAA GATTTTCATC GGACATTTGG CTGTGATGAT GAGTTAATGT 
     4321 ATGGGGGAGT TTCTTCTTAT GAAGGTTCCA TGGCTCTCAA CGCTAGACCT CAGAGCCCAC 
     4381 GAGAAGGGCA TATGATGGAT GCTTTGTATG CCCAAGTCAA GAAGCCGCGG AATTCCAAAC 
     4441 CCTCACCTGT AGACAGTAAC AGATCAACTC CTAGCAATCA TGATCGGATA CAGCGTCTGA 
     4501 GGCAAGAATT TCAGCAAGCA AAGCAAGATG AAGATGTAGA AGATCGTCGG CGGACCTATA 
     4561 GTTTTGAGCA ACCCTGGCCG AACGCACGGC CGGCGACGCA GAGCGGGCGA CACTCGGTGT 
     4621 CCGTGGAGGT GCAGATGCAG CGGCAGCGGC AGGAGGAGCG CGAGAGCTCC CAGCAGGCCC 
     4681 AGCGCCAGTA CAGCTCTCTG CCTCGGCAAA GCAGGAAAAA TGCCAGCTCG GTCTCCCAGG 
     4741 ACTCTTGGGA GCAGAACTAC TCCCCTGGGG AAGGCTTCCA GAGTGCCAAA GAGAACCCCA 
     4801 GGTACTCCAG CTACCAAGGC TCCAGGAACG GCTACCTGGG AGGACATGGC TTCAACGCCA 
     4861 GGGTCATGCT GGAAACTCAG GAGCTCCTTC GCCAGGAACA GAGGCGGAAG GAGCAGCAGA 
     4921 TGAAGAAGCA GCCTCCTTCC GAGGGGCCCA GCAACTATGA CTCGTATAAG AAAGTCCAGG 
     4981 ACCCCAGTTA CGCCCCTCCC AAGGGGCCCT TCCGGCAAGA TGTGCCCCCC TCCCCTTCTC 
     5041 AGGTTGCGAG GCTGAACAGA CTTCAGACTC CTGAGAAAGG GAGGCCCTTC TATTCCACGC 
     5101 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     5161 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     5221 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     5281 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     5341 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     5401 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     5461 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     5521 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     5581 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     5641 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     5701 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     5761 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     5821 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     5881 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     5941 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     6001 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     6061 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     6121 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     6181 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     6241 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     6301 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     6361 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     6421 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     6481 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     6541 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     6601 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     6661 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     6721 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     6781 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     6841 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     6901 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     6961 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     7021 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     7081 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     7141 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     7201 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     7261 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     7321 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     7381 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     7441 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     7501 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     7561 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     7621 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     7681 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     7741 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     7801 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     7861 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     7921 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     7981 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     8041 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     8101 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     8161 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     8221 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     8281 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     8341 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     8401 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     8461 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     8521 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     8581 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     8641 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     8701 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     8761 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     8821 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     8881 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     8941 GCGAATTTT
//