LOCUS       RC223680                8229 bp    DNA     circular  SYN 
DEFINITION  SLC12A5 (Myc-DDK-tagged)-Human solute carrier family 12 (potassium/chloride transporter), member 5 (SLC12A5), transcript variant 2
ACCESSION   NM_020708
VERSION     NM_020708.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4386..4477 
                     /label="Myc-DDK"  
     misc_feature    4507..5092 
                     /label="PolyA signal"  
     misc_feature    4564..4583 
                     /label="Restriction Enzyme"  
     misc_feature    5368..6040 
                     /label="ColE1"  
     misc_feature    6470..7264 
                     /label="Kan/Neo"  
     misc_feature    7299..7601 
                     /label="SV40 ori"  
     misc_feature    7687..8164 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4368..4374 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4368 
                     /label="SLC12A5"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCTAAACAAC CTGACGGACT GCGAGGACGG CGATGGGGGA GCCAACCCGG 
     1081 GTGATGGCAA CCCCAAGGAA AGCAGTCCCT TCATCAACAG CACCGACACA GAGAAGGGAA 
     1141 AGGAGTATGA TGGCAAGAAC ATGGCCTTGT TTGAGGAGGA GATGGACACC AGCCCTATGG 
     1201 TGTCCTCCTT GCTCAGTGGC CTGGCCAACT ACACCAACCT GCCCCAGGGA AGTAGGGAGC 
     1261 ATGAAGAGGC AGAAAACAAT GAGGGTGGAA AAAAGAAGCC GGTGCAGGCC CCACGCATGG 
     1321 GCACCTTCAT GGGCGTGTAC CTGCCGTGCC TGCAGAACAT CTTTGGCGTC ATCCTCTTCC 
     1381 TGCGGCTCAC CTGGGTGGTG GGCATTGCAG GCATCATGGA GTCCTTCTGC ATGGTGTTCA 
     1441 TCTGCTGCTC CTGTACGATG CTCACGGCCA TCTCCATGAG TGCAATTGCA ACGAATGGTG 
     1501 TTGTGCCTGC TGGTGGCTCC TACTACATGA TTTCCAGGTC TCTGGGCCCA GAGTTTGGGG 
     1561 GTGCCGTGGG CCTCTGCTTC TACCTGGGCA CTACCTTTGC AGGAGCCATG TACATCCTGG 
     1621 GCACCATCGA AATCCTGCTG GCTTACCTCT TCCCAGCCAT GGCCATCTTC AAGGCAGAAG 
     1681 ATGCCAGTGG GGAGGCAGCA GCCATGCTGA ACAACATGCG TGTTTACGGC ACCTGTGTGC 
     1741 TCACCTGCAT GGCCACTGTG GTGTTTGTGG GTGTCAAGTA TGTCAACAAG TTTGCCCTTG 
     1801 TCTTCCTGGG TTGTGTCATC CTCTCCATCC TGGCCATCTA TGCTGGGGTC ATCAAGTCTG 
     1861 CCTTCGACCC ACCCAACTTC CCGATCTGCC TCCTGGGTAA CCGCACGCTG TCTCGCCATG 
     1921 GCTTTGATGT CTGTGCCAAG CTGGCTTGGG AAGGAAATGA GACGGTGACC ACACGGCTAT 
     1981 GGGGCCTTTT CTGCTCCTCT CGCTTCCTCA ACGCCACCTG TGATGAATAC TTCACCCGAA 
     2041 ACAATGTCAC AGAGATCCAG GGCATCCCTG GTGCTGCCAG TGGCCTCATC AAAGAGAACC 
     2101 TCTGGAGCTC CTACCTGACC AAGGGCGTGA TTGTGGAGAG GAGTGGGATG ACCTCGGTGG 
     2161 GCCTGGCCGA TGGCACTCCT ATCGACATGG ACCACCCTTA TGTCTTCAGT GATATGACCT 
     2221 CCTACTTCAC CCTGCTGGTT GGCATCTACT TCCCCTCAGT CACAGGGATC ATGGCTGGTT 
     2281 CTAACCGCTC TGGGGACCTG AGGGATGCCC AGAAGTCAAT CCCCACTGGC ACCATCCTGG 
     2341 CCATCGCCAC CACCTCTGCT GTCTACATCA GCTCCGTTGT TCTGTTTGGG GCCTGCATTG 
     2401 AGGGGGTCGT CCTGCGGGAC AAGTTTGGCG AAGCTGTGAA TGGCAACCTC GTGGTGGGCA 
     2461 CTCTGGCCTG GCCATCTCCA TGGGTAATTG TCATCGGATC CTTCTTCTCC ACCTGTGGGG 
     2521 CTGGGCTGCA GAGCCTCACG GGGGCCCCAC GCCTGCTGCA GGCCATCTCG AGGGATGGCA 
     2581 TTGTGCCCTT CCTGCAGGTC TTTGGCCATG GCAAGGCCAA TGGAGAGCCG ACCTGGGCCC 
     2641 TGCTCCTGAC TGCCTGCATC TGCGAGATTG GCATCCTCAT TGCATCCCTC GACGAGGTGG 
     2701 CCCCCATCCT CTCTATGTTC TTCCTGATGT GCTACATGTT TGTGAATCTG GCCTGTGCAG 
     2761 TGCAGACGCT GCTGAGGACA CCCAACTGGA GGCCACGCTT TCGATATTAC CACTGGACCC 
     2821 TCTCCTTCCT GGGCATGAGC CTCTGCCTGG CCCTCATGTT CATCTGCTCC TGGTATTATG 
     2881 CACTGGTAGC CATGCTCATT GCTGGACTCA TCTACAAGTA CATTGAGTAC CGTGGGGCAG 
     2941 AGAAGGAGTG GGGCGATGGG ATACGAGGTC TGTCTCTCAG TGCGGCTCGC TATGCCCTCT 
     3001 TACGCCTGGA GGAAGGGCCC CCACACACCA AGAACTGGAG GCCACAGCTG CTGGTGCTGG 
     3061 TGCGTGTGGA CCAAGACCAG AATGTGGTGC ACCCCCAGCT GCTCTCACTG ACCTCCCAGC 
     3121 TGAAGGCAGG GAAGGGCCTG ACCATCGTGG GCTCTGTCCT TGAGGGCACC TTTCTGGAAA 
     3181 ATCATCCACA GGCCCAGCGG GCAGAAGAGT CTATCAGGCG CCTGATGGAG GCAGAGAAGG 
     3241 TGAAGGGCTT CTGCCAGGTG GTGATCTCCT CCAACTTGCG TGATGGCGTG TCCCATCTGA 
     3301 TCCAGTCCGG GGGCCTCGGG GGGCTGCAGC ACAACACTGT GCTTGTTGGC TGGCCCCGCA 
     3361 ACTGGCGCCA GAAGGAAGAT CATCAGACAT GGAGGAACTT CATTGAGCTG GTCCGGGAAA 
     3421 CCACAGCTGG CCACTTAGCC CTGCTGGTCA CCAAGAACGT TTCCATGTTT CCTGGGAACC 
     3481 CTGAGCGCTT CTCTGAGGGC AGCATCGACG TTTGGTGGAT TGTGCACGAT GGAGGCATGC 
     3541 TCATGCTGCT GCCCTTCCTG CTGCGGCACC ACAAGGTCTG GCGGAAGTGC AAGATGCGTA 
     3601 TCTTCACTGT GGCCCAGATG GATGACAATA GCATCCAGAT GAAGAAGGAT CTGACCACAT 
     3661 TTCTGTATCA TTTACGCATC ACTGCGGAGG TCGAGGTGGT GGAGATGCAT GAGAGCGACA 
     3721 TCTCAGCTTA CACCTATGAG AAGACGTTGG TGATGGAGCA GCGTTCCCAG ATCCTCAAAC 
     3781 AGATGCATTT AACCAAGAAT GAGCGGGAGC GGGAGATCCA GAGTATCACA GATGAGTCAC 
     3841 GAGGCTCAAT CCGGAGAAAG AATCCAGCCA ACACGCGGCT CCGCCTGAAC GTCCCAGAAG 
     3901 AGACGGCTGG TGACAGTGAA GAGAAGCCAG AGGAGGAGGT GCAGCTGATC CACGATCAGA 
     3961 GTGCTCCCAG CTGCCCCAGC AGCTCCCCGT CCCCAGGGGA GGAGCCTGAG GGGGAAGGGG 
     4021 AGACAGATCC GGAGAAGGTG CATCTCACCT GGACCAAGGA CAAGTCGGTG GCAGAGAAGA 
     4081 ATAAGGGCCC CAGTCCTGTC TCCTCTGAGG GCATCAAGGA CTTCTTCAGC ATGAAGCCGG 
     4141 AGTGGGAGAA CTTGAACCAG TCCAACGTGC GGCGCATGCA CACGGCCGTG CGGCTGAACG 
     4201 AGGTCATCGT GAAGAAATCC CGGGACGCCA AGCTTGTTTT GCTCAACATG CCTGGGCCTC 
     4261 CCCGCAACCG CAATGGTGAT GAAAACTACA TGGAGTTTCT CGAGGTCCTC ACAGAGCACC 
     4321 TGGACCGGGT GATGCTGGTC CGCGGCGGCG GCCGCGAGGT CATCACCATC TACTCCACGC 
     4381 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     4441 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     4501 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     4561 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     4621 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     4681 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     4741 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     4801 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     4861 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     4921 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     4981 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     5041 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     5101 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     5161 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     5221 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     5281 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     5341 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     5401 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     5461 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     5521 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     5581 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     5641 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     5701 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     5761 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     5821 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     5881 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     5941 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     6001 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     6061 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     6121 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     6181 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     6241 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     6301 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     6361 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     6421 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     6481 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     6541 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     6601 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     6661 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     6721 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     6781 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     6841 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     6901 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     6961 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     7021 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     7081 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     7141 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     7201 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     7261 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     7321 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     7381 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     7441 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     7501 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     7561 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7621 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     7681 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     7741 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     7801 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     7861 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     7921 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     7981 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     8041 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     8101 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     8161 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     8221 GCGAATTTT
//