LOCUS       RC223673                7617 bp    DNA     circular  SYN 
DEFINITION  GRM4 (Myc-DDK-tagged)-Human glutamate receptor, metabotropic 4 (GRM4)
ACCESSION   NM_000841
VERSION     NM_000841.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3774..3865 
                     /label="Myc-DDK"  
     misc_feature    3895..4480 
                     /label="PolyA signal"  
     misc_feature    3952..3971 
                     /label="Restriction Enzyme"  
     misc_feature    4756..5428 
                     /label="ColE1"  
     misc_feature    5858..6652 
                     /label="Kan/Neo"  
     misc_feature    6687..6989 
                     /label="SV40 ori"  
     misc_feature    7075..7552 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3756..3762 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3756 
                     /label="GRM4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCTGGGAAG AGAGGCTTGG GCTGGTGGTG GGCCCGGCTG CCCCTTTGCC 
     1081 TGCTCCTCAG CCTTTACGGC CCCTGGATGC CTTCCTCCCT GGGAAAGCCC AAAGGCCACC 
     1141 CTCACATGAA TTCCATCCGC ATAGATGGGG ACATCACACT GGGAGGCCTG TTCCCGGTGC 
     1201 ATGGCCGGGG CTCAGAGGGC AAGCCCTGTG GAGAACTTAA GAAGGAAAAG GGCATCCACC 
     1261 GGCTGGAGGC CATGCTGTTC GCCCTGGATC GCATCAACAA CGACCCGGAC CTGCTGCCTA 
     1321 ACATCACGCT GGGCGCCCGC ATTCTGGACA CCTGCTCCAG GGACACCCAT GCCCTCGAGC 
     1381 AGTCGCTGAC CTTTGTGCAG GCGCTCATCG AGAAGGATGG CACAGAGGTC CGCTGTGGCA 
     1441 GTGGCGGCCC ACCCATCATC ACCAAGCCTG AACGTGTGGT GGGTGTCATC GGTGCTTCAG 
     1501 GGAGCTCGGT CTCCATCATG GTGGCCAACA TCCTTCGCCT CTTCAAGATA CCCCAGATCA 
     1561 GCTACGCCTC CACAGCGCCA GACCTGAGTG ACAACAGCCG CTACGATTTC TTCTCCCGCG 
     1621 TGGTGCCCTC GGACACGTAC CAGGCCCAGG CCATGGTGGA CATCGTCCGT GCCCTCAAGT 
     1681 GGAACTATGT GTCCACAGTG GCCTCGGAGG GCAGCTATGG TGAGAGCGGT GTGGAGGCCT 
     1741 TCATCCAGAA GTCCCGTGAG GACGGGGGCG TGTGCATCGC CCAGTCGGTG AAGATACCAC 
     1801 GGGAGCCCAA GGCAGGCGAG TTCGACAAGA TCATCCGCCG CCTCCTGGAG ACTTCGAACG 
     1861 CCAGGGCAGT CATCATCTTT GCCAACGAGG ATGACATCAG GCGTGTGCTG GAGGCAGCAC 
     1921 GAAGGGCCAA CCAGACAGGC CATTTCTTCT GGATGGGCTC TGACAGCTGG GGCTCCAAGA 
     1981 TTGCACCTGT GCTGCACCTG GAGGAGGTGG CTGAGGGTGC TGTCACGATC CTCCCCAAGA 
     2041 GGATGTCCGT ACGAGGCTTC GACCGCTACT TCTCCAGCCG CACGCTGGAC AACAACCGGC 
     2101 GCAACATCTG GTTTGCCGAG TTCTGGGAGG ACAACTTCCA CTGCAAGCTG AGCCGCCACG 
     2161 CCCTCAAGAA GGGCAGCCAC GTCAAGAAGT GCACCAACCG TGAGCGAATT GGGCAGGATT 
     2221 CAGCTTATGA GCAGGAGGGG AAGGTGCAGT TTGTGATCGA TGCCGTGTAC GCCATGGGCC 
     2281 ACGCGCTGCA CGCCATGCAC CGTGACCTGT GTCCCGGCCG CGTGGGGCTC TGCCCGCGCA 
     2341 TGGACCCTGT AGATGGCACC CAGCTGCTTA AGTACATCCG AAACGTCAAC TTCTCAGGCA 
     2401 TCGCAGGGAA CCCTGTGACC TTCAATGAGA ATGGAGATGC GCCTGGGCGC TATGACATCT 
     2461 ACCAATACCA GCTGCGCAAC GATTCTGCCG AGTACAAGGT CATTGGCTCC TGGACTGACC 
     2521 ACCTGCACCT TAGAATAGAG CGGATGCACT GGCCGGGGAG CGGGCAGCAG CTGCCCCGCT 
     2581 CCATCTGCAG CCTGCCCTGC CAACCGGGTG AGCGGAAGAA GACAGTGAAG GGCATGCCTT 
     2641 GCTGCTGGCA CTGCGAGCCT TGCACAGGGT ACCAGTACCA GGTGGACCGC TACACCTGTA 
     2701 AGACGTGTCC CTATGACATG CGGCCCACAG AGAACCGCAC GGGCTGCCGG CCCATCCCCA 
     2761 TCATCAAGCT TGAGTGGGGC TCGCCCTGGG CCGTGCTGCC CCTCTTCCTG GCCGTGGTGG 
     2821 GCATCGCTGC CACGTTGTTC GTGGTGATCA CCTTTGTGCG CTACAACGAC ACGCCCATCG 
     2881 TCAAGGCCTC GGGCCGTGAA CTGAGCTACG TGCTGCTGGC AGGCATCTTC CTGTGCTATG 
     2941 CCACCACCTT CCTCATGATC GCTGAGCCCG ACCTTGGCAC CTGCTCGCTG CGCCGAATCT 
     3001 TCCTGGGACT AGGGATGAGC ATCAGCTATG CAGCCCTGCT CACCAAGACC AACCGCATCT 
     3061 ACCGCATCTT CGAGCAGGGC AAGCGCTCGG TCAGTGCCCC ACGCTTCATC AGCCCCGCCT 
     3121 CACAGCTGGC CATCACCTTC AGCCTCATCT CGCTGCAGCT GCTGGGCATC TGTGTGTGGT 
     3181 TTGTGGTGGA CCCCTCCCAC TCGGTGGTGG ACTTCCAGGA CCAGCGGACA CTCGACCCCC 
     3241 GCTTCGCCAG GGGTGTGCTC AAGTGTGACA TCTCGGACCT GTCGCTCATC TGCCTGCTGG 
     3301 GCTACAGCAT GCTGCTCATG GTCACGTGCA CCGTGTATGC CATCAAGACA CGCGGCGTGC 
     3361 CCGAGACCTT CAATGAGGCC AAGCCCATTG GCTTCACCAT GTACACCACT TGCATCGTCT 
     3421 GGCTGGCCTT CATCCCCATC TTCTTTGGCA CCTCGCAGTC GGCCGACAAG CTGTACATCC 
     3481 AGACGACGAC GCTGACGGTC TCGGTGAGTC TGAGCGCCTC GGTGTCCCTG GGAATGCTCT 
     3541 ACATGCCCAA AGTCTACATC ATCCTCTTCC ACCCGGAGCA GAATGTGCCC AAGCGCAAGC 
     3601 GCAGCCTCAA AGCCGTCGTT ACGGCGGCCA CCATGTCCAA CAAGTTCACG CAGAAGGGCA 
     3661 ACTTCCGGCC CAACGGAGAG GCCAAGTCTG AGCTCTGCGA GAACCTTGAG GCCCCAGCGC 
     3721 TGGCCACCAA ACAGACTTAC GTCACTTACA CCAACCATGC AATCACGCGT ACGCGGCCGC 
     3781 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     3841 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     3901 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     3961 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     4021 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     4081 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     4141 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     4201 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     4261 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     4321 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     4381 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     4441 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     4501 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     4561 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     4621 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     4681 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     4741 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     4801 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     4861 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     4921 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     4981 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     5041 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     5101 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     5161 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     5221 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     5281 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     5341 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     5401 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     5461 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     5521 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     5581 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     5641 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     5701 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     5761 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     5821 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     5881 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     5941 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     6001 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     6061 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     6121 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     6181 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     6241 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     6301 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     6361 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     6421 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     6481 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     6541 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     6601 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     6661 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     6721 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     6781 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     6841 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     6901 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     6961 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     7021 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     7081 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     7141 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     7201 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     7261 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     7321 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     7381 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     7441 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     7501 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     7561 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//