LOCUS       RC223646                9210 bp    DNA     circular  SYN 
DEFINITION  NLRP1 (Myc-DDK-tagged)-Human NLR family, pyrin domain containing 1 (NLRP1), transcript variant 3
ACCESSION   NM_033006
VERSION     NM_033006.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5367..5458 
                     /label="Myc-DDK"  
     misc_feature    5488..6073 
                     /label="PolyA signal"  
     misc_feature    5545..5564 
                     /label="Restriction Enzyme"  
     misc_feature    6349..7021 
                     /label="ColE1"  
     misc_feature    7451..8245 
                     /label="Kan/Neo"  
     misc_feature    8280..8582 
                     /label="SV40 ori"  
     misc_feature    8668..9145 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5349..5355 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5349 
                     /label="NLRP1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTGGCGGA GCCTGGGGCC GCCTGGCCTG TTACTTGGAG TTCCTGAAGA 
     1081 AGGAGGAGCT GAAGGAGTTC CAGCTTCTGC TCGCCAATAA AGCGCACTCC AGGAGCTCTT 
     1141 CGGGTGAGAC ACCCGCTCAG CCAGAGAAGA CGAGTGGCAT GGAGGTGGCC TCGTACCTGG 
     1201 TGGCTCAGTA TGGGGAGCAG CGGGCCTGGG ACCTAGCCCT CCATACCTGG GAGCAGATGG 
     1261 GGCTGAGGTC ACTGTGCGCC CAAGCCCAGG AAGGGGCAGG CCACTCTCCC TCATTCCCCT 
     1321 ACAGCCCAAG TGAACCCCAC CTGGGGTCTC CCAGCCAACC CACCTCCACC GCAGTGCTAA 
     1381 TGCCCTGGAT CCATGAATTG CCGGCGGGGT GCACCCAGGG CTCAGAGAGA AGGGTTTTGA 
     1441 GACAGCTGCC TGACACATCT GGACGCCGCT GGAGAGAAAT CTCTGCCTCA CTCCTCTACC 
     1501 AAGCTCTTCC AAGCTCCCCA GACCATGAGT CTCCAAGCCA GGAGTCACCC AACGCCCCCA 
     1561 CATCCACAGC AGTGCTGGGG AGCTGGGGAT CCCCACCTCA GCCCAGCCTA GCACCCAGAG 
     1621 AGCAGGAGGC TCCTGGGACC CAATGGCCTC TGGATGAAAC GTCAGGAATT TACTACACAG 
     1681 AAATCAGAGA AAGAGAGAGA GAGAAATCAG AGAAAGGCAG GCCCCCATGG GCAGCGGTGG 
     1741 TAGGAACGCC CCCACAGGCG CACACCAGCC TACAGCCCCA CCACCACCCA TGGGAGCCTT 
     1801 CTGTGAGAGA GAGCCTCTGT TCCACATGGC CCTGGAAAAA TGAGGATTTT AACCAAAAAT 
     1861 TCACACAGCT GCTACTTCTA CAAAGACCTC ACCCCAGAAG CCAAGATCCC CTGGTCAAGA 
     1921 GAAGCTGGCC TGATTATGTG GAGGAGAATC GAGGACATTT AATTGAGATC AGAGACTTAT 
     1981 TTGGCCCAGG CCTGGATACC CAAGAACCTC GCATAGTCAT ACTGCAGGGG GCTGCTGGAA 
     2041 TTGGGAAGTC AACACTGGCC AGGCAGGTGA AGGAAGCCTG GGGGAGAGGC CAGCTGTATG 
     2101 GGGACCGCTT CCAGCATGTC TTCTACTTCA GCTGCAGAGA GCTGGCCCAG TCCAAGGTGG 
     2161 TGAGTCTCGC TGAGCTCATC GGAAAAGATG GGACAGCCAC TCCGGCTCCC ATTAGACAGA 
     2221 TCCTGTCTAG GCCAGAGCGG CTGCTCTTCA TCCTCGATGG TGTAGATGAG CCAGGATGGG 
     2281 TCTTGCAGGA GCCGAGTTCT GAGCTCTGTC TGCACTGGAG CCAGCCACAG CCGGCGGATG 
     2341 CACTGCTGGG CAGTTTGCTG GGGAAAACTA TACTTCCCGA GGCATCCTTC CTGATCACGG 
     2401 CTCGGACCAC AGCTCTGCAG AACCTCATTC CTTCTTTGGA GCAGGCACGT TGGGTAGAGG 
     2461 TCCTGGGGTT CTCTGAGTCC AGCAGGAAGG AATATTTCTA CAGATATTTC ACAGATGAAA 
     2521 GGCAAGCAAT TAGAGCCTTT AGGTTGGTCA AATCAAACAA AGAGCTCTGG GCCCTGTGTC 
     2581 TTGTGCCCTG GGTGTCCTGG CTGGCCTGCA CTTGCCTGAT GCAGCAGATG AAGCGGAAGG 
     2641 AAAAACTCAC ACTGACTTCC AAGACCACCA CAACCCTCTG TCTACATTAC CTTGCCCAGG 
     2701 CTCTCCAAGC TCAGCCATTG GGACCCCAGC TCAGAGACCT CTGCTCTCTG GCTGCTGAGG 
     2761 GCATCTGGCA AAAAAAGACC CTTTTCAGTC CAGATGACCT CAGGAAGCAT GGGTTAGATG 
     2821 GGGCCATCAT CTCCACCTTC TTGAAGATGG GTATTCTTCA AGAGCACCCC ATCCCTCTGA 
     2881 GCTACAGCTT CATTCACCTC TGTTTCCAAG AGTTCTTTGC AGCAATGTCC TATGTCTTGG 
     2941 AGGATGAGAA GGGGAGAGGT AAACATTCTA ATTGCATCAT AGATTTGGAA AAGACGCTAG 
     3001 AAGCATATGG AATACATGGC CTGTTTGGGG CATCAACCAC ACGTTTCCTA TTGGGCCTGT 
     3061 TAAGTGATGA GGGGGAGAGA GAGATGGAGA ACATCTTTCA CTGCCGGCTG TCTCAGGGGA 
     3121 GGAACCTGAT GCAGTGGGTC CCGTCCCTGC AGCTGCTGCT GCAGCCACAC TCTCTGGAGT 
     3181 CCCTCCACTG CTTGTACGAG ACTCGGAACA AAACGTTCCT GACACAAGTG ATGGCCCATT 
     3241 TCGAAGAAAT GGGCATGTGT GTAGAAACAG ACATGGAGCT CTTAGTGTGC ACTTTCTGCA 
     3301 TTAAATTCAG CCGCCACGTG AAGAAGCTTC AGCTGATTGA GGGCAGGCAG CACAGATCAA 
     3361 CATGGAGCCC CACCATGGTA GTCCTGTTCA GGTGGGTCCC AGTCACAGAT GCCTATTGGC 
     3421 AGATTCTCTT CTCCGTCCTC AAGGTCACCA GAAACCTGAA GGAGCTGGAC CTAAGTGGAA 
     3481 ACTCGCTGAG CCACTCTGCA GTGAAGAGTC TTTGTAAGAC CCTGAGACGC CCTCGCTGCC 
     3541 TCCTGGAGAC CCTGCGGTTG GCTGGCTGTG GCCTCACAGC TGAGGACTGC AAGGACCTTG 
     3601 CCTTTGGGCT GAGAGCCAAC CAGACCCTGA CCGAGCTGGA CCTGAGCTTC AATGTGCTCA 
     3661 CGGATGCTGG AGCCAAACAC CTTTGCCAGA GACTGAGACA GCCGAGCTGC AAGCTACAGC 
     3721 GACTGCAGCT GGTCAGCTGT GGCCTCACGT CTGACTGCTG CCAGGACCTG GCCTCTGTGC 
     3781 TTAGTGCCAG CCCCAGCCTG AAGGAGCTAG ACCTGCAGCA GAACAACCTG GATGACGTTG 
     3841 GCGTGCGACT GCTCTGTGAG GGGCTCAGGC ATCCTGCCTG CAAACTCATA CGCCTGGGGA 
     3901 AACCAAGTGT GATGACCCCT ACTGAGGGCC TGGATACGGG AGAGATGAGT AATAGCACAT 
     3961 CCTCACTCAA GCGGCAGAGA CTCGGATCAG AGAGGGCGGC TTCCCATGTT GCTCAGGCTA 
     4021 ATCTCAAACT CCTGGACGTG AGCAAGATCT TCCCAATTGC TGAGATTGCA GAGGAAAGCT 
     4081 CCCCAGAGGT AGTACCGGTG GAACTCTTGT GCGTGCCTTC TCCTGCCTCT CAAGGGGACC 
     4141 TGCATACGAA GCCTTTGGGG ACTGACGATG ACTTCTGGGG CCCCACGGGG CCTGTGGCTA 
     4201 CTGAGGTAGT TGACAAAGAA AAGAACTTGT ACCGAGTTCA CTTCCCTGTA GCTGGCTCCT 
     4261 ACCGCTGGCC CAACACGGGT CTCTGCTTTG TGATGAGAGA AGCGGTGACC GTTGAGATTG 
     4321 AATTCTGTGT GTGGGACCAG TTCCTGGGTG AGATCAACCC ACAGCACAGC TGGATGGTGG 
     4381 CAGGGCCTCT GCTGGACATC AAGGCTGAGC CTGGAGCTGT GGAAGCTGTG CACCTCCCTC 
     4441 ACTTTGTGGC TCTCCAAGGG GGCCATGTGG ACACATCCCT GTTCCAAATG GCCCACTTTA 
     4501 AAGAGGAGGG GATGCTCCTG GAGAAGCCAG CCAGGGTGGA GCTGCATCAC ATAGTTCTGG 
     4561 AAAACCCCAG CTTCTCCCCC TTGGGAGTCC TCCTGAAAAT GATCCATAAT GCCCTGCGCT 
     4621 TCATTCCCGT CACCTCTGTG GTGTTGCTTT ACCACCGCGT CCATCCTGAG GAAGTCACCT 
     4681 TCCACCTCTA CCTGATCCCA AGTGACTGCT CCATTCGGAA GGCCATAGAT GATCTAGAAA 
     4741 TGAAATTCCA GTTTGTGCGA ATCCACAAGC CACCCCCGCT GACCCCACTT TATATGGGCT 
     4801 GTCGTTACAC TGTGTCTGGG TCTGGTTCAG GGATGCTGGA AATACTCCCC AAGGAACTGG 
     4861 AGCTCTGCTA TCGAAGCCCT GGAGAAGACC AGCTGTTCTC GGAGTTCTAC GTTGGCCACT 
     4921 TGGGATCAGG GATCAGGCTG CAAGTGAAAG ACAAGAAAGA TGAGACTCTG GTGTGGGAGG 
     4981 CCTTGGTGAA ACCAGGAGAT CTCATGCCTG CAACTACTCT GATCCCTCCA GCCCGCATAG 
     5041 CCGTACCTTC ACCTCTGGAT GCCCCGCAGT TGCTGCACTT TGTGGACCAG TATCGAGAGC 
     5101 AGCTGATAGC CCGAGTGACA TCGGTGGAGG TTGTCTTGGA CAAACTGCAT GGACAGGTGC 
     5161 TGAGCCAGGA GCAGTACGAG AGGGTGCTGG CTGAGAACAC GAGGCCCAGC CAGATGCGGA 
     5221 AGCTGTTCAG CTTGAGCCAG TCCTGGGACC GGAAGTGCAA AGATGGACTC TACCAAGCCC 
     5281 TGAAGGAGAC CCATCCTCAC CTCATTATGG AACTCTGGGA GAAGGGCAGC AAAAAGGGAC 
     5341 TCCTGCCACT CAGCAGCACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     5401 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     5461 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     5521 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     5581 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     5641 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     5701 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     5761 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     5821 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     5881 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     5941 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     6001 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     6061 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     6121 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     6181 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     6241 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     6301 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     6361 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     6421 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     6481 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     6541 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     6601 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     6661 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     6721 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     6781 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     6841 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     6901 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     6961 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     7021 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     7081 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     7141 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     7201 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     7261 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     7321 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     7381 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     7441 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     7501 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     7561 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     7621 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     7681 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     7741 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     7801 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     7861 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     7921 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     7981 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     8041 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     8101 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     8161 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     8221 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     8281 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     8341 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     8401 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     8461 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     8521 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     8581 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     8641 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     8701 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     8761 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     8821 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     8881 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     8941 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     9001 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     9061 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     9121 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     9181 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//