LOCUS       RC223637L1                8985 bp    DNA     circular  SYN 
DEFINITION  DNM2 (Myc-DDK-tagged)-Human dynamin 2 (DNM2), transcript variant 2
ACCESSION   NM_001005361
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7276..7456 
                     /label="3'LTR"  
     misc_feature    833..1013 
                     /label="5'LTR"  
     misc_feature    8184..8843 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV promoter"  
     misc_feature    7615..8183 
                     /label="ColE1"  
     misc_feature    6151..6171 
                     /label="LR50 primer"  
     misc_feature    5948..6019 
                     /label="Myc-DDK"  
     misc_feature    3252..3271 
                     /label="T7 promoter"  
     misc_feature    3221..3237 
                     /label="V2 primer"  
     misc_feature    6160..6751 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    5930..5936 
                     /label="Restriction Enzyme"  
     misc_feature    3320..5930 
                     /label="DNM2"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GGGCAACCGC GGGATGGAAG AGCTGATCCC 
     3361 GCTGGTCAAC AAACTGCAGG ACGCCTTCAG CTCCATCGGC CAGAGCTGCC ACCTGGACCT 
     3421 GCCGCAGATC GCTGTAGTGG GCGGCCAGAG CGCCGGCAAG AGCTCGGTGC TGGAGAACTT 
     3481 CGTGGGCCGG GACTTCCTTC CCCGCGGTTC AGGAATCGTC ACCCGGCGGC CTCTCATTCT 
     3541 GCAGCTCATC TTCTCAAAAA CAGAACATGC CGAGTTTTTG CACTGCAAGT CCAAAAAGTT 
     3601 TACAGACTTT GATGAAGTCC GGCAGGAGAT TGAAGCAGAG ACCGACAGGG TCACGGGGAC 
     3661 CAACAAAGGC ATCTCCCCAG TGCCCATCAA CCTTCGAGTC TACTCGCCAC ACGTGTTGAA 
     3721 CTTGACCCTC ATCGACCTCC CGGGTATCAC CAAGGTGCCT GTGGGCGACC AGCCTCCAGA 
     3781 CATCGAGTAC CAGATCAAGG ACATGATCCT GCAGTTCATC AGCCGGGAGA GCAGCCTCAT 
     3841 TCTGGCTGTC ACGCCCGCCA ACATGGACCT GGCCAACTCC GACGCCCTCA AGCTGGCCAA 
     3901 GGAAGTCGAT CCCCAAGGCC TACGGACCAT CGGTGTCATC ACCAAGCTTG ACCTGATGGA 
     3961 CGAGGGCACC GACGCCAGGG ACGTCTTGGA GAACAAGTTG CTCCCGTTGA GAAGAGGCTA 
     4021 CATTGGCGTG GTGAACCGCA GCCAGAAGGA TATTGAGGGC AAGAAGGACA TCCGTGCAGC 
     4081 ACTGGCAGCT GAGAGGAAGT TCTTCCTCTC CCACCCGGCC TACCGGCACA TGGCCGACCG 
     4141 CATGGGCACG CCACATCTGC AGAAGACGCT GAATCAGCAA CTGACCAACC ACATCCGGGA 
     4201 GTCGCTGCCG GCCCTACGTA GCAAACTACA GAGCCAGCTG CTGTCCCTGG AGAAGGAGGT 
     4261 GGAGGAGTAC AAGAACTTTC GGCCCGACGA CCCCACCCGC AAAACCAAAG CCCTGCTGCA 
     4321 GATGGTCCAG CAGTTTGGGG TGGATTTTGA GAAGAGGATC GAGGGCTCAG GAGATCAGGT 
     4381 GGACACTCTG GAGCTCTCCG GGGGCGCCCG AATCAATCGC ATCTTCCACG AGCGGTTCCC 
     4441 ATTTGAGCTG GTGAAGATGG AGTTTGACGA GAAGGACTTA CGACGGGAGA TCAGCTATGC 
     4501 CATTAAGAAC ATCCATGGAG TCAGGACGGG GCTCTTCACC CCCGACATGG CCTTTGAAGC 
     4561 CATTGTGAAA AAACAGATTG TAAAACTCAA AGAGCCGAGT TTGAAGTGTG TTGATCTCGT 
     4621 GGTCTCAGAG CTGGCCACGG TCATAAAAAA GTGTGCCGAG AAGCTCAGTT CCTACCCCCG 
     4681 GTTGCGAGAG GAGACAGAGC GAATCGTCAC CACTTACATC CGGGAACGGG AGGGGAGAAC 
     4741 GAAGGACCAG ATTCTTCTGC TGATCGACAT TGAGCAGTCC TACATCAACA CGAACCATGA 
     4801 GGACTTCATC GGGTTTGCCA ATGCCCAGCA GAGGAGCACG CAGCTGAACA AGAAGAGAGC 
     4861 CATCCCCAAT CAGGGGGAGA TCCTGGTGAT CCGCAGGGGC TGGCTGACCA TCAACAACAT 
     4921 CAGCCTGATG AAAGGCGGCT CCAAGGAGTA CTGGTTTGTG CTGACTGCCG AGTCACTGTC 
     4981 CTGGTACAAG GATGAGGAGG AGAAAGAGAA GAAGTACATG CTGCCTCTGG ACAACCTCAA 
     5041 GATCCGTGAT GTGGAGAAGG GCTTCATGTC CAACAAGCAC GTCTTCGCCA TCTTCAACAC 
     5101 GGAGCAGAGA AACGTCTACA AGGACCTGCG GCAGATCGAG CTGGCCTGTG ACTCCCAGGA 
     5161 AGACGTGGAC AGCTGGAAGG CCTCGTTCCT CCGAGCTGGC GTCTACCCCG AGAAGGACCA 
     5221 GGCAGAAAAC GAGGATGGGG CCCAGGAGAA CACCTTCTCC ATGGACCCCC AACTGGAGCG 
     5281 GCAGGTGGAG ACCATTCGCA ACCTGGTGGA CTCATACGTG GCCATCATCA ACAAGTCCAT 
     5341 CCGCGACCTC ATGCCAAAGA CCATCATGCA CCTCATGATC AACAATACGA AGGCCTTCAT 
     5401 CCACCACGAG CTGCTGGCCT ACCTATACTC CTCGGCAGAC CAGAGCAGCC TCATGGAGGA 
     5461 GTCGGCTGAC CAGGCACAGC GGCGGGACGA CATGCTGCGC ATGTACCATG CCCTCAAGGA 
     5521 GGCGCTCAAC ATCATCGGTG ACATCAGCAC CAGCACTGTG TCCACGCCTG TACCCCCGCC 
     5581 TGTCGATGAC ACCTGGCTCC AGAGCGCCAG CAGCCACAGC CCCACTCCAC AGCGCCGACC 
     5641 GGTGTCCAGC ATACACCCCC CTGGCCGGCC CCCAGCAGTG AGGGGCCCCA CTCCAGGGCC 
     5701 CCCCCTGATT CCTGTTCCCG TGGGGGCAGC AGCCTCCTTC TCGGCGCCCC CAATCCCATC 
     5761 CCGGCCTGGA CCCCAGAGCG TGTTTGCCAA CAGTGACCTC TTCCCAGCCC CGCCTCAGAT 
     5821 CCCATCTCGG CCAGTTCGGA TCCCCCCAGG GATTCCCCCA GGAGTGCCCA GCAGAAGACC 
     5881 CCCTGCTGCG CCCAGCCGGC CCACCATTAT CCGCCCAGCC GAGCCATCCC TGCTCGACAC 
     5941 GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT 
     6001 CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC TGTACAAGTA 
     6061 GGATTCGTCG AGGGACCTAA TAACTTCGTA TAGCATACAT TATACGAAGT TATACATGTT 
     6121 TAAGGGTTCC GGTTCCACTA GGTACAATTC GATATCAAGC TTATCGATAA TCAACCTCTG 
     6181 GATTACAAAA TTTGTGAAAG ATTGACTGGT ATTCTTAACT ATGTTGCTCC TTTTACGCTA 
     6241 TGTGGATACG CTGCTTTAAT GCCTTTGTAT CATGCTATTG CTTCCCGTAT GGCTTTCATT 
     6301 TTCTCCTCCT TGTATAAATC CTGGTTGCTG TCTCTTTATG AGGAGTTGTG GCCCGTTGTC 
     6361 AGGCAACGTG GCGTGGTGTG CACTGTGTTT GCTGACGCAA CCCCCACTGG TTGGGGCATT 
     6421 GCCACCACCT GTCAGCTCCT TTCCGGGACT TTCGCTTTCC CCCTCCCTAT TGCCACGGCG 
     6481 GAACTCATCG CCGCCTGCCT TGCCCGCTGC TGGACAGGGG CTCGGCTGTT GGGCACTGAC 
     6541 AATTCCGTGG TGTTGTCGGG GAAATCATCG TCCTTTCCTT GGCTGCTCGC CTGTGTTGCC 
     6601 ACCTGGATTC TGCGCGGGAC GTCCTTCTGC TACGTCCCTT CGGCCCTCAA TCCAGCGGAC 
     6661 CTTCCTTCCC GCGGCCTGCT GCCGGCTCTG CGGCCTCTTC CGCGTCTTCG CCTTCGCCCT 
     6721 CAGACGAGTC GGATCTCCCT TTGGGCCGCC TCCCCGCATC GATACCGTCG ACCTCGATCG 
     6781 AGACCTAGAA AAACATGGAG CAATCACAAG TAGCAATACA GCAGCTACCA ATGCTGATTG 
     6841 TGCCTGGCTA GAAGCACAAG AGGAGGAGGA GGTGGGTTTT CCAGTCACAC CTCAGGTACC 
     6901 TTTAAGACCA ATGACTTACA AGGCAGCTGT AGATCTTAGC CACTTTTTAA AAGAAAAGGG 
     6961 GGGACTGGAA GGGCTAATTC ACTCCCAACG AAGACAAGAT ATCCTTGATC TGTGGATCTA 
     7021 CCACACACAA GGCTACTTCC CTGATTGGCA GAACTACACA CCAGGGCCAG GGATCAGATA 
     7081 TCCACTGACC TTTGGATGGT GCTACAAGCT AGTACCAGTT GAGCAAGAGA AGGTAGAAGA 
     7141 AGCCAATGAA GGAGAGAACA CCCGCTTGTT ACACCCTGTG AGCCTGCATG GGATGGATGA 
     7201 CCCGGAGAGA GAAGTATTAG AGTGGAGGTT TGACAGCCGC CTAGCATTTC ATCACATGGC 
     7261 CCGAGAGCTG CATCCGGACT GTACTGGGTC TCTCTGGTTA GACCAGATCT GAGCCTGGGA 
     7321 GCTCTCTGGC TAACTAGGGA ACCCACTGCT TAAGCCTCAA TAAAGCTTGC CTTGAGTGCT 
     7381 TCAAGTAGTG TGTGCCCGTC TGTTGTGTGA CTCTGGTAAC TAGAGATCCC TCAGACCCTT 
     7441 TTAGTCAGTG TGGAAAATCT CTAGCAGCAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     7501 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     7561 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     7621 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     7681 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     7741 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     7801 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     7861 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     7921 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     7981 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     8041 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     8101 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     8161 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GATTACGCCC CGCCCTGCCA CTCATCGCAG 
     8221 TACTGTTGTA ATTCATTAAG CATTCTGCCG ACATGGAAGC CATCACAAAC GGCATGATGA 
     8281 ACCTGAATCG CCAGCGGCAT CAGCACCTTG TCGCCTTGCG TATAATATTT GCCCATGGTG 
     8341 AAAACGGGGG CGAAGAAGTT GTCCATATTG GCCACGTTTA AATCAAAACT GGTGAAACTC 
     8401 ACCCAGGGAT TGGCTGAGAC GAAAAACATA TTCTCAATAA ACCCTTTAGG GAAATAGGCC 
     8461 AGGTTTTCAC CGTAACACGC CACATCTTGC GAATATATGT GTAGAAACTG CCGGAAATCG 
     8521 TCGTGGTATT CACTCCAGAG CGATGAAAAC GTTTCAGTTT GCTCATGGAA AACGGTGTAA 
     8581 CAAGGGTGAA CACTATCCCA TATCACCAGC TCACCGTCTT TCATTGCCAT ACGGAACTCC 
     8641 GGATGAGCAT TCATCAGGCG GGCAAGAATG TGAATAAAGG CCGGATAAAA CTTGTGCTTA 
     8701 TTTTTCTTTA CGGTCTTTAA AAAGGCCGTA ATATCCAGCT GAACGGTCTG GTTATAGGTA 
     8761 CATTGAGCAA CTGACTGAAA TGCCTCAAAA TGTTCTTTAC GATGCCATTG GGATATATCA 
     8821 ACGGTGGTAT ATCCAGTGAT TTTTTTCTCC ATACTCTTCC TTTTTCAATA TTATTGAAGC 
     8881 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     8941 CAAATAGGGG TCCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGAC
//