LOCUS       RC223635                9276 bp    DNA     circular  SYN 
DEFINITION  ATP7B (Myc-DDK-tagged)-Human ATPase, Cu++ transporting, beta polypeptide (ATP7B), transcript variant 1
ACCESSION   NM_000053
VERSION     NM_000053.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5433..5524 
                     /label="Myc-DDK"  
     misc_feature    5554..6139 
                     /label="PolyA signal"  
     misc_feature    5611..5630 
                     /label="Restriction Enzyme"  
     misc_feature    6415..7087 
                     /label="ColE1"  
     misc_feature    7517..8311 
                     /label="Kan/Neo"  
     misc_feature    8346..8648 
                     /label="SV40 ori"  
     misc_feature    8734..9211 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5415..5421 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5415 
                     /label="ATP7B"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCTGAGCAG GAGAGACAGA TCACAGCCAG AGAAGGGGCC AGTCGGAAAA 
     1081 TCTTATCTAA GCTTTCTTTG CCTACCCGTG CCTGGGAACC AGCAATGAAG AAGAGTTTTG 
     1141 CTTTTGACAA TGTTGGCTAT GAAGGTGGTC TGGATGGCCT GGGCCCTTCT TCTCAGGTGG 
     1201 CCACCAGCAC AGTCAGGATC TTGGGCATGA CTTGCCAGTC ATGTGTGAAG TCCATTGAGG 
     1261 ACAGGATTTC CAATTTGAAA GGCATCATCA GCATGAAGGT TTCCCTGGAA CAAGGCAGTG 
     1321 CCACTGTGAA ATATGTGCCA TCGGTTGTGT GCCTGCAACA GGTTTGCCAT CAAATTGGGG 
     1381 ACATGGGCTT CGAGGCCAGC ATTGCAGAAG GAAAGGCAGC CTCCTGGCCC TCAAGGTCCT 
     1441 TGCCTGCCCA GGAGGCTGTG GTCAAGCTCC GGGTGGAGGG CATGACCTGC CAGTCCTGTG 
     1501 TCAGCTCCAT TGAAGGCAAG GTCCGGAAAC TGCAAGGAGT AGTGAGAGTC AAAGTCTCAC 
     1561 TCAGCAACCA AGAGGCCGTC ATCACTTATC AGCCTTATCT CATTCAGCCC GAAGACCTCA 
     1621 GGGACCATGT AAATGACATG GGATTTGAAG CTGCCATCAA GAGCAAAGTG GCTCCCTTAA 
     1681 GCCTGGGACC AATTGATATT GAGCGGTTAC AAAGCACTAA CCCAAAGAGA CCTTTATCTT 
     1741 CTGCTAACCA GAATTTTAAT AATTCTGAGA CCTTGGGGCA CCAAGGAAGC CATGTGGTCA 
     1801 CCCTCCAACT GAGAATAGAT GGAATGCATT GTAAGTCTTG CGTCTTGAAT ATTGAAGAAA 
     1861 ATATTGGCCA GCTCCTAGGG GTTCAAAGTA TTCAAGTGTC CTTGGAGAAC AAAACTGCCC 
     1921 AAGTAAAGTA TGACCCTTCT TGTACCAGCC CAGTGGCTCT GCAGAGGGCT ATCGAGGCAC 
     1981 TTCCACCTGG GAATTTTAAA GTTTCTCTTC CTGATGGAGC CGAAGGGAGT GGGACAGATC 
     2041 ACAGGTCTTC CAGTTCTCAT TCCCCTGGCT CCCCACCGAG AAACCAGGTC CAGGGCACAT 
     2101 GCAGTACCAC TCTGATTGCC ATTGCCGGCA TGACCTGTGC ATCCTGTGTC CATTCCATTG 
     2161 AAGGCATGAT CTCCCAACTG GAAGGGGTGC AGCAAATATC GGTGTCTTTG GCCGAAGGGA 
     2221 CTGCAACAGT TCTTTATAAT CCCGCTGTAA TTAGCCCAGA AGAACTCAGA GCTGCTATAG 
     2281 AAGACATGGG ATTTGAGGCT TCAGTCGTTT CTGAAAGCTG TTCTACTAAC CCTCTTGGAA 
     2341 ACCACAGTGC TGGGAATTCC ATGGTGCAAA CTACAGATGG TACACCTACA TCTCTGCAGG 
     2401 AAGTGGCTCC CCACACTGGG AGGCTCCCTG CAAACCATGC CCCGGACATC TTGGCAAAGT 
     2461 CCCCACAATC AACCAGAGCA GTGGCACCGC AGAAGTGCTT CTTACAGATC AAAGGCATGA 
     2521 CCTGTGCATC CTGTGTGTCT AACATAGAAA GGAATCTGCA GAAAGAAGCT GGTGTTCTCT 
     2581 CCGTGTTGGT TGCCTTGATG GCAGGAAAGG CAGAGATCAA GTATGACCCA GAGGTCATCC 
     2641 AGCCCCTCGA GATAGCTCAG TTCATCCAGG ACCTGGGTTT TGAGGCAGCA GTCATGGAGG 
     2701 ACTACGCAGG CTCCGATGGC AACATTGAGC TGACAATCAC AGGGATGACC TGCGCGTCCT 
     2761 GTGTCCACAA CATAGAGTCC AAACTCACGA GGACAAATGG CATCACTTAT GCCTCCGTTG 
     2821 CCCTTGCCAC CAGCAAAGCC CTTGTTAAGT TTGACCCGGA AATTATCGGT CCACGGGATA 
     2881 TTATCAAAAT TATTGAGGAA ATTGGCTTTC ATGCTTCCCT GGCCCAGAGA AACCCCAACG 
     2941 CTCATCACTT GGACCACAAG ATGGAAATAA AGCAGTGGAA GAAGTCTTTC CTGTGCAGCC 
     3001 TGGTGTTTGG CATCCCTGTC ATGGCCTTAA TGATCTATAT GCTGATACCC AGCAACGAGC 
     3061 CCCACCAGTC CATGGTCCTG GACCACAACA TCATTCCAGG ACTGTCCATT CTAAATCTCA 
     3121 TCTTCTTTAT CTTGTGTACC TTTGTCCAGC TCCTCGGTGG GTGGTACTTC TACGTTCAGG 
     3181 CCTACAAATC TCTGAGACAC AGGTCAGCCA ACATGGACGT GCTCATCGTC CTGGCCACAA 
     3241 GCATTGCTTA TGTTTATTCT CTGGTCATCC TGGTGGTTGC TGTGGCTGAG AAGGCGGAGA 
     3301 GGAGCCCTGT GACATTCTTC GACACGCCCC CCATGCTCTT TGTGTTCATT GCCCTGGGCC 
     3361 GGTGGCTGGA ACACTTGGCA AAGAGCAAAA CCTCAGAAGC CCTGGCTAAA CTCATGTCTC 
     3421 TCCAAGCCAC AGAAGCCACC GTTGTGACCC TTGGTGAGGA CAATTTAATC ATCAGGGAGG 
     3481 AGCAAGTCCC CATGGAGCTG GTGCAGCGGG GCGATATCGT CAGGGTGGTC CCTGGGGGAA 
     3541 AGTTTCCAGT GGATGGGAAA GTCCTGGAAG GCAATACCAT GGCTGATGAG TCCCTCATCA 
     3601 CAGGAGAAGC CATGCCAGTC ACTAAGAAAC CCGGAAGCAC TGTAATTGCG GGGTCTATAA 
     3661 ATGCACATGG CTCTGTGCTC ATTAAAGCTA CCCACGTGGG CAATGACACC ACTTTGGCTC 
     3721 AGATTGTGAA ACTGGTGGAA GAGGCTCAGA TGTCAAAGGC ACCCATTCAG CAGCTGGCTG 
     3781 ACCGGTTTAG TGGATATTTT GTCCCATTTA TCATCATCAT GTCAACTTTG ACGTTGGTGG 
     3841 TATGGATTGT AATCGGTTTT ATCGATTTTG GTGTTGTTCA GAAATACTTT CCTAACCCCA 
     3901 ACAAGCACAT CTCCCAGACA GAGGTGATCA TCCGGTTTGC TTTCCAGACG TCCATCACGG 
     3961 TGCTGTGCAT TGCCTGCCCC TGCTCCCTGG GGCTGGCCAC GCCCACGGCT GTCATGGTGG 
     4021 GCACCGGGGT GGCCGCGCAG AACGGCATCC TCATCAAGGG AGGCAAGCCC CTGGAGATGG 
     4081 CGCACAAGAT AAAGACTGTG ATGTTTGACA AGACTGGCAC CATTACCCAT GGCGTCCCCA 
     4141 GGGTCATGCG GGTGCTCCTG CTGGGGGATG TGGCCACACT GCCCCTCAGG AAGGTTCTGG 
     4201 CTGTGGTGGG GACTGCGGAG GCCAGCAGTG AACACCCCTT GGGCGTGGCA GTCACCAAAT 
     4261 ACTGTAAAGA GGAACTTGGA ACAGAGACCT TGGGATACTG CACGGACTTC CAGGCAGTGC 
     4321 CAGGCTGTGG AATTGGGTGC AAAGTCAGCA ACGTGGAAGG CATCCTGGCC CACAGTGAGC 
     4381 GCCCTTTGAG TGCACCGGCC AGTCACCTGA ATGAGGCTGG CAGCCTTCCC GCAGAAAAAG 
     4441 ATGCAGCCCC CCAGACCTTC TCTGTGCTGA TTGGAAACCG TGAGTGGCTG AGGCGCAACG 
     4501 GTTTAACCAT TTCTAGCGAT GTCAGTGACG CTATGACAGA CCACGAGATG AAAGGACAGA 
     4561 CAGCCATCCT GGTGGCTATT GACGGTGTGC TCTGTGGGAT GATCGCAATC GCAGACGCTG 
     4621 TCAAGCAGGA GGCTGCCCTG GCTGTGCACA CGCTGCAGAG CATGGGTGTG GACGTGGTTC 
     4681 TGATCACGGG GGACAACCGG AAGACAGCCA GAGCTATTGC CACCCAGGTT GGCATCAACA 
     4741 AAGTCTTTGC AGAGGTGCTG CCTTCGCACA AGGTGGCCAA GGTCCAGGAG CTCCAGAATA 
     4801 AAGGGAAGAA AGTCGCCATG GTGGGGGATG GGGTCAATGA CTCCCCGGCC TTGGCCCAGG 
     4861 CAGACATGGG TGTGGCCATT GGCACCGGCA CGGATGTGGC CATCGAGGCA GCCGACGTCG 
     4921 TCCTTATCAG AAATGATTTG CTGGATGTGG TGGCTAGCAT TCACCTTTCC AAGAGGACTG 
     4981 TCCGAAGGAT ACGCATCAAC CTGGTCCTGG CACTGATTTA TAACCTGGTT GGGATACCCA 
     5041 TTGCAGCAGG TGTCTTCATG CCCATCGGCA TTGTGCTGCA GCCCTGGATG GGCTCAGCGG 
     5101 CCATGGCAGC CTCCTCTGTG TCTGTGGTGC TCTCATCCCT GCAGCTCAAG TGCTATAAGA 
     5161 AGCCTGACCT GGAGAGGTAT GAGGCACAGG CGCATGGCCA CATGAAGCCC CTGACGGCAT 
     5221 CCCAGGTCAG TGTGCACATA GGCATGGATG ACAGGTGGCG GGACTCCCCC AGGGCCACAC 
     5281 CATGGGACCA GGTCAGCTAT GTCAGCCAGG TGTCGCTGTC CTCCCTGACG TCCGACAAGC 
     5341 CATCTCGGCA CAGCGCTGCA GCAGACGATG ATGGGGACAA GTGGTCTCTG CTCCTGAATG 
     5401 GCAGGGATGA GGAGCAGTAC ATCACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     5461 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     5521 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     5581 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     5641 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     5701 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     5761 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     5821 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     5881 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     5941 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     6001 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     6061 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     6121 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     6181 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     6241 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     6301 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     6361 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     6421 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     6481 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     6541 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     6601 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     6661 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     6721 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     6781 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     6841 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     6901 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     6961 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     7021 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     7081 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     7141 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     7201 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     7261 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     7321 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     7381 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     7441 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     7501 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     7561 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     7621 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     7681 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     7741 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     7801 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     7861 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     7921 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     7981 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     8041 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     8101 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     8161 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     8221 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     8281 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     8341 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     8401 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     8461 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     8521 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     8581 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     8641 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     8701 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     8761 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     8821 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     8881 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     8941 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     9001 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     9061 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     9121 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     9181 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     9241 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//