LOCUS       RC223623                9333 bp    DNA     circular  SYN 
DEFINITION  GRIN2B (Myc-DDK-tagged)-Human glutamate receptor, ionotropic, N-methyl D-aspartate 2B (GRIN2B)
ACCESSION   NM_000834
VERSION     NM_000834.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5490..5581 
                     /label="Myc-DDK"  
     misc_feature    5611..6196 
                     /label="PolyA signal"  
     misc_feature    5668..5687 
                     /label="Restriction Enzyme"  
     misc_feature    6472..7144 
                     /label="ColE1"  
     misc_feature    7574..8368 
                     /label="Kan/Neo"  
     misc_feature    8403..8705 
                     /label="SV40 ori"  
     misc_feature    8791..9268 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5472..5478 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5472 
                     /label="GRIN2B"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAGCCCAGA GCGGAGTGCT GTTCTCCCAA GTTCTGGTTG GTGTTGGCCG 
     1081 TCCTGGCCGT GTCAGGCAGC AGAGCTCGTT CTCAGAAGAG CCCCCCCAGC ATTGGCATTG 
     1141 CTGTCATCCT CGTGGGCACT TCCGACGAGG TGGCCATCAA GGATGCCCAC GAGAAAGATG 
     1201 ATTTCCACCA TCTCTCCGTG GTACCCCGGG TGGAACTGGT AGCCATGAAT GAGACCGACC 
     1261 CAAAGAGCAT CATCACCCGC ATCTGTGATC TCATGTCTGA CCGGAAGATC CAGGGGGTGG 
     1321 TGTTTGCTGA TGACACAGAC CAGGAAGCCA TCGCCCAGAT CCTCGATTTC ATTTCAGCAC 
     1381 AGACTCTCAC CCCGATCCTG GGCATCCACG GGGGCTCCTC TATGATAATG GCAGATAAGG 
     1441 ATGAATCCTC CATGTTCTTC CAGTTTGGCC CATCAATTGA ACAGCAAGCT TCCGTAATGC 
     1501 TCAACATCAT GGAAGAATAT GACTGGTACA TCTTTTCTAT CGTCACCACC TATTTCCCTG 
     1561 GCTACCAGGA CTTTGTAAAC AAGATCCGCA GCACCATTGA GAATAGCTTT GTGGGCTGGG 
     1621 AGCTAGAGGA GGTCCTCCTA CTGGACATGT CCCTGGACGA TGGAGATTCT AAGATCCAGA 
     1681 ATCAGCTCAA GAAACTTCAA AGCCCCATCA TTCTTCTTTA CTGTACCAAG GAAGAAGCCA 
     1741 CCTACATCTT TGAAGTGGCC AACTCAGTAG GGCTGACTGG CTATGGCTAC ACGTGGATCG 
     1801 TGCCCAGTCT GGTGGCAGGG GATACAGACA CAGTGCCTGC GGAGTTCCCC ACTGGGCTCA 
     1861 TCTCTGTATC ATATGATGAA TGGGACTATG GCCTCCCCGC CAGAGTGAGA GATGGAATTG 
     1921 CCATAATCAC CACTGCTGCT TCTGACATGC TGTCTGAGCA CAGCTTCATC CCTGAGCCCA 
     1981 AAAGCAGTTG TTACAACACC CACGAGAAGA GAATCTACCA GTCCAATATG CTAAATAGGT 
     2041 ATCTGATCAA TGTCACTTTT GAGGGGAGGA ATTTGTCCTT CAGTGAAGAT GGCTACCAGA 
     2101 TGCACCCGAA ACTGGTGATA ATTCTTCTGA ACAAGGAGAG GAAGTGGGAA AGGGTGGGGA 
     2161 AGTGGAAAGA CAAGTCCCTG CAGATGAAGT ACTATGTGTG GCCCCGAATG TGTCCAGAGA 
     2221 CTGAAGAGCA GGAGGATGAC CATCTGAGCA TTGTGACCCT GGAGGAGGCA CCATTTGTCA 
     2281 TTGTGGAAAG TGTGGACCCT CTGAGTGGAA CCTGCATGAG GAACACAGTC CCCTGCCAAA 
     2341 AACGCATAGT CACTGAGAAT AAAACAGACG AGGAGCCGGG TTACATCAAA AAATGCTGCA 
     2401 AGGGGTTCTG TATTGACATC CTTAAGAAAA TTTCTAAATC TGTGAAGTTC ACCTATGACC 
     2461 TTTACCTGGT TACCAATGGC AAGCATGGGA AGAAAATCAA TGGAACCTGG AATGGTATGA 
     2521 TTGGAGAGGT GGTCATGAAG AGGGCCTACA TGGCAGTGGG CTCACTCACC ATCAATGAGG 
     2581 AACGATCGGA GGTGGTCGAC TTCTCTGTGC CCTTCATAGA GACAGGCATC AGTGTCATGG 
     2641 TGTCACGCAG CAATGGGACT GTCTCACCTT CTGCCTTCTT AGAGCCATTC AGCGCTGACG 
     2701 TATGGGTGAT GATGTTTGTG ATGCTGCTCA TCGTCTCAGC CGTGGCTGTC TTTGTCTTTG 
     2761 AGTACTTCAG CCCTGTGGGT TATAACAGGT GCCTCGCTGA TGGCAGAGAG CCTGGTGGAC 
     2821 CCTCTTTCAC CATCGGCAAA GCTATTTGGT TGCTCTGGGG TCTGGTGTTT AACAACTCCG 
     2881 TACCTGTGCA GAACCCAAAG GGGACCACCT CCAAGATCAT GGTGTCAGTG TGGGCCTTCT 
     2941 TTGCTGTCAT CTTCCTGGCC AGCTACACTG CCAACTTAGC TGCCTTCATG ATCCAAGAGG 
     3001 AATATGTGGA CCAGGTTTCT GGCCTGAGCG ACAAAAAGTT CCAGAGACCT AATGACTTCT 
     3061 CACCCCCTTT CCGCTTTGGG ACCGTGCCCA ACGGCAGCAC AGAGAGAAAT ATTCGCAATA 
     3121 ACTATGCAGA AATGCATGCC TACATGGGAA AGTTCAACCA GAGGGGTGTA GATGATGCAT 
     3181 TGCTCTCCCT GAAAACAGGG AAACTGGATG CCTTCATCTA TGATGCAGCA GTGCTGAACT 
     3241 ATATGGCAGG CAGAGATGAA GGCTGCAAGC TGGTGACCAT TGGCAGTGGG AAGGTCTTTG 
     3301 CTTCCACTGG CTATGGCATT GCCATCCAAA AAGATTCTGG GTGGAAGCGC CAGGTGGACC 
     3361 TTGCTATCCT GCAGCTCTTT GGAGATGGGG AGATGGAAGA ACTGGAAGCT CTCTGGCTCA 
     3421 CTGGCATTTG TCACAATGAG AAGAATGAGG TCATGAGCAG CCAGCTGGAC ATTGACAACA 
     3481 TGGCAGGGGT CTTCTACATG TTGGGGGCGG CCATGGCTCT CAGCCTCATC ACCTTCATCT 
     3541 GCGAACACCT TTTCTATTGG CAGTTCCGAC ATTGCTTTAT GGGTGTCTGT TCTGGCAAGC 
     3601 CTGGCATGGT CTTCTCCATC AGCAGAGGTA TCTACAGCTG CATCCATGGG GTGGCGATCG 
     3661 AGGAGCGCCA GTCTGTAATG AACTCCCCCA CTGCAACCAT GAACAACACA CACTCCAACA 
     3721 TCCTGCGCCT GCTGCGCACG GCCAAGAACA TGGCTAACCT GTCTGGTGTG AATGGCTCAC 
     3781 CGCAGAGCGC CCTGGACTTC ATCCGACGGG AGTCATCCGT CTATGACATC TCAGAGCACC 
     3841 GCCGCAGCTT CACGCATTCT GACTGCAAAT CCTACAACAA CCCGCCCTGT GAGGAGAACC 
     3901 TCTTCAGTGA CTACATCAGT GAGGTAGAGA GAACGTTCGG GAACCTGCAG CTGAAGGACA 
     3961 GCAACGTGTA CCAAGATCAC TACCACCATC ACCACCGGCC CCATAGTATT GGCAGTGCCA 
     4021 GCTCCATCGA TGGGCTCTAC GACTGTGACA ACCCACCCTT CACCACCCAG TCCAGGTCCA 
     4081 TCAGCAAGAA GCCCCTGGAC ATCGGCCTCC CCTCCTCCAA GCACAGCCAG CTCAGTGACC 
     4141 TGTACGGCAA ATTCTCCTTC AAGAGCGACC GCTACAGTGG CCACGACGAC TTGATCCGCT 
     4201 CCGATGTCTC TGACATCTCA ACCCACACCG TCACCTATGG GAACATCGAG GGCAATGCCG 
     4261 CCAAGAGGCG TAAGCAGCAA TATAAGGACA GCCTGAAGAA GCGGCCTGCC TCGGCCAAGT 
     4321 CCCGCAGGGA GTTTGACGAG ATCGAGCTGG CCTACCGTCG CCGACCGCCC CGCTCCCCTG 
     4381 ACCACAAGCG CTACTTCAGG GACAAGGAAG GGCTACGGGA CTTCTACCTG GACCAGTTCC 
     4441 GAACAAAGGA GAACTCACCC CACTGGGAGC ACGTAGACCT GACCGACATC TACAAGGAGC 
     4501 GGAGTGATGA CTTTAAGCGC GACTCCGTCA GCGGAGGAGG GCCCTGTACC AACAGGTCTC 
     4561 ACATCAAGCA CGGGACGGGC GACAAACACG GCGTGGTCAG CGGGGTACCT GCACCTTGGG 
     4621 AGAAGAACCT GACCAACGTG GAGTGGGAGG ACCGGTCCGG GGGCAACTTC TGCCGCAGCT 
     4681 GTCCCTCCAA GCTGCACAAC TACTCCACGA CGGTGACGGG TCAGAACTCG GGCAGGCAGG 
     4741 CGTGCATCCG GTGTGAGGCT TGCAAGAAAG CAGGCAACCT GTATGACATC AGTGAGGACA 
     4801 ACTCCCTGCA GGAACTGGAC CAGCCGGCTG CCCCAGTGGC GGTGACGTCA AACGCCTCCA 
     4861 CCACTAAGTA CCCTCAGAGC CCGACTAATT CCAAGGCCCA GAAGAAGAAC CGGAACAAAC 
     4921 TGCGCCGGCA GCACTCCTAC GACACCTTCG TGGACCTGCA GAAGGAAGAA GCCGCCCTGG 
     4981 CCCCGCGCAG CGTAAGCCTG AAAGACAAGG GCCGATTCAT GGATGGGAGC CCCTACGCCC 
     5041 ACATGTTTGA GATGTCAGCT GGCGAGAGCA CCTTTGCCAA CAACAAGTCC TCAGTGCCCA 
     5101 CTGCCGGACA TCACCACCAC AACAACCCCG GCGGCGGGTA CATGCTCAGC AAGTCGCTCT 
     5161 ACCCTGACCG GGTCACGCAA AACCCTTTCA TCCCCACTTT TGGGGACGAC CAGTGCTTGC 
     5221 TCCATGGCAG CAAATCCTAC TTCTTCAGGC AGCCCACGGT GGCGGGGGCG TCGAAAGCCA 
     5281 GGCCGGACTT CCGGGCCCTT GTCACCAACA AGCCGGTGGT CTCGGCCCTT CATGGGGCCG 
     5341 TGCCAGCCCG TTTCCAGAAG GACATCTGTA TAGGGAACCA GTCCAACCCC TGTGTGCCTA 
     5401 ACAACAAAAA CCCCAGGGCT TTCAATGGCT CCAGCAATGG GCATGTTTAT GAGAAACTTT 
     5461 CTAGTATTGA GTCTGATGTC ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     5521 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     5581 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     5641 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     5701 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     5761 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     5821 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     5881 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     5941 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     6001 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     6061 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     6121 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     6181 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     6241 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     6301 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     6361 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     6421 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     6481 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     6541 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     6601 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     6661 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     6721 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     6781 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     6841 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     6901 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     6961 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     7021 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     7081 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     7141 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     7201 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     7261 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     7321 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     7381 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     7441 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     7501 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     7561 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     7621 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     7681 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     7741 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     7801 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     7861 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     7921 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     7981 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     8041 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     8101 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     8161 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     8221 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     8281 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     8341 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     8401 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     8461 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     8521 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     8581 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     8641 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     8701 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     8761 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     8821 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     8881 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     8941 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     9001 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     9061 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     9121 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     9181 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     9241 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     9301 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//