LOCUS       RC223617                6561 bp    DNA     circular  SYN 
DEFINITION  GPR123 (Myc-DDK-tagged)-Human G protein-coupled receptor 123 (GPR123)
ACCESSION   NM_001083909
VERSION     NM_001083909.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2718..2809 
                     /label="Myc-DDK"  
     misc_feature    2839..3424 
                     /label="PolyA signal"  
     misc_feature    2896..2915 
                     /label="Restriction Enzyme"  
     misc_feature    3700..4372 
                     /label="ColE1"  
     misc_feature    4802..5596 
                     /label="Kan/Neo"  
     misc_feature    5631..5933 
                     /label="SV40 ori"  
     misc_feature    6019..6496 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2700..2706 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2700 
                     /label="ADGRA1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGATCTGAAG ACAGTGCTCT CCCTGCCCCG CTACCCAGGG GAGTTCCTGC 
     1081 ACCCCGTGGT GTACGCGTGC ACGGCCGTCA TGCTGCTCTG CCTCCTGGCC TCCTTCGTCA 
     1141 CCTACATCGT GCACCAGAGC GCCATCCGCA TCAGCCGCAA GGGCCGGCAC ACGCTCCTGA 
     1201 ATTTCTGCTT CCACGCGGCC CTGACCTTCA CTGTGTTCGC CGGCGGCATC AATCGCACCA 
     1261 AGTACCCCAT CCTGTGCCAG GCGGTGGGCA TCGTGCTGCA CTATTCTACA CTGTCCACCA 
     1321 TGCTGTGGAT AGGAGTGACC GCCAGGAACA TCTACAAGCA GGTGACCAAG AAGGCCCCTC 
     1381 TGTGCCTGGA CACAGACCAG CCACCGTACC CCAGGCAGCC CCTGCTCAGG TTTTACCTCG 
     1441 TCAGCGGAGG GGTCCCCTTT ATCATCTGTG GGGTCACGGC TGCCACGAAC ATCAGGAATT 
     1501 ACGGGACAGA GGACGAGGAC ACGGCGTACT GCTGGATGGC CTGGGAGCCC AGCCTGGGCG 
     1561 CCTTCTACGG CCCAGCCGCC ATCATCACCC TGGTCACCTG TGTGTACTTC CTGGGCACCT 
     1621 ACGTGCAGCT GCGGCGCCAC CCAGGGCGCA GGTACGAGCT GCGCACACAG CCCGAGGAGC 
     1681 AGCGGCGGCT GGCGACACCC GAGGGCGGCC GTGGGATCCG GCCAGGCACC CCACCCGCAC 
     1741 ACGATGCCCC CGGCGCCTCC GTGCTGCAGA ACGAGCACTC ATTCCAGGCA CAGCTGCGCG 
     1801 CCGCCGCCTT CACGCTGTTC CTGTTCACGG CCACGTGGGC CTTCGGGGCG CTGGCGGTGT 
     1861 CACAGGGCCA CTTCCTGGAC ATGGTCTTCA GCTGCCTGTA CGGCGCCTTC TGCGTGACCC 
     1921 TGGGACTCTT CGTGCTCATC CACCACTGCG CCAAGCGTGA GGACGTGTGG CAGTGCTGGT 
     1981 GGGCATGCTG CCCGCCCCGC AAGGACGCCC ACCCCGCACT TGACGCCAAC GGGGCCGCGC 
     2041 TGGGCCGCGC CGCCTGCCTG CACTCGCCGG GACTGGGCCA GCCACGGGGC TTCGCGCACC 
     2101 CACCGGGCCC CTGCAAGATG ACCAACCTGC AGGCCGCGCA GGGCCACGCC AGTTGCCTGT 
     2161 CACCGGCCAC CCCGTGCTGC GCCAAGATGC ACTGCGAGCC ACTGACGGCG GACGAGGCGC 
     2221 ACGTGCACCT GCAGGAGGAG GGCGCCTTCG GGCACGACCC CCACCTGCAC GGGTGCCTTC 
     2281 AGGGCAGAAC TAAGCCGCCC TACTTTAGCC GGCACCCAGC AGAGGAGCCC GAGTACGCCT 
     2341 ACCACATCCC ATCCAGCCTG GATGGCAGCC CCCGCAGCTC GCGCACAGAC AGCCCCCCCA 
     2401 GCTCTCTGGA TGGCCCGGCG GGGACACACA CGCTGGCCTG CTGCACCCAG GGCGACCCCT 
     2461 TCCCCATGGT CACCCAGCCC GAGGGCAGTG ATGGGAGCCC TGCCCTCTAC AGCTGCCCCA 
     2521 CGCAGCCGGG CAGGGAGGCA GCGCTCGGGC CCGGCCACTT GGAGATGCTG CGGAGGACAC 
     2581 AGTCCCTGCC CTTTGGTGGC CCCAGCCAGA ACGGGCTGCC CAAGGGTAAA TTGCTAGAAG 
     2641 GCCTGCCGTT TGGCACCGAC GGGACCGGCA ACATCCGAAC GGGACCCTGG AAAAACGAAA 
     2701 CTACTGTGAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     2761 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     2821 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     2881 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     2941 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     3001 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     3061 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     3121 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     3181 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     3241 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     3301 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     3361 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     3421 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     3481 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     3541 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     3601 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     3661 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     3721 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     3781 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     3841 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     3901 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     3961 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     4021 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     4081 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     4141 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     4201 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     4261 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     4321 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     4381 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     4441 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     4501 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     4561 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     4621 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     4681 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     4741 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     4801 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     4861 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     4921 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     4981 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     5041 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     5101 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     5161 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     5221 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     5281 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     5341 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     5401 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     5461 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     5521 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     5581 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     5641 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     5701 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     5761 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     5821 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     5881 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     5941 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     6001 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     6061 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     6121 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     6181 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     6241 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     6301 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     6361 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     6421 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     6481 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     6541 CAAAAATTTA ACGCGAATTT T
//