LOCUS       RC223556                8313 bp    DNA     circular  SYN 
DEFINITION  DCTN1 (Myc-DDK-tagged)-Human dynactin 1 (DCTN1), transcript variant 2
ACCESSION   NM_023019
VERSION     NM_023019.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4470..4561 
                     /label="Myc-DDK"  
     misc_feature    4591..5176 
                     /label="PolyA signal"  
     misc_feature    4648..4667 
                     /label="Restriction Enzyme"  
     misc_feature    5452..6124 
                     /label="ColE1"  
     misc_feature    6554..7348 
                     /label="Kan/Neo"  
     misc_feature    7383..7685 
                     /label="SV40 ori"  
     misc_feature    7771..8248 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4452..4458 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4452 
                     /label="DCTN1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATGAGACAG GCACCGACAG CCCGAAAGAC CACAACTCGG CGACCCAAGC 
     1081 CCACGCGCCC AGCCAGTACT GGGGTGGCTG GGGCCAGTAG CTCCCTGGGC CCCTCTGGCT 
     1141 CAGCGTCAGC AGGTGAGCTG AGCAGCAGTG AGCCCAGCAC CCCGGCTCAG ACTCCGCTGG 
     1201 CAGCACCCAT CATCCCCACG CCGGTCCTCA CCTCTCCTGG AGCAGTCCCC CCGCTTCCTT 
     1261 CCCCATCCAA GGAGGAGGAG GGACTAAGGG CTCAGGTGCG GGACCTGGAG GAGAAACTAG 
     1321 AGACCCTGAG ACTGAAACGG GCAGAAGACA AAGCAAAGCT AAAAGAGCTG GAGAAACACA 
     1381 AAATCCAGCT GGAGCAGGTG CAGGAATGGA AGAGCAAAAT GCAGGAGCAG CAGGCCGACC 
     1441 TGCAGCGGCG CCTCAAGGAG GCGAGAAAGG AAGCCAAGGA GGCGCTGGAG GCAAAGGAAC 
     1501 GCTATATGGA GGAGATGGCT GATACTGCTG ATGCCATTGA GATGGCCACT TTGGACAAGG 
     1561 AGATGGCTGA AGAGCGGGCT GAGTCCCTGC AGCAGGAGGT GGAGGCACTG AAGGAGCGGG 
     1621 TGGACGAGCT CACTACTGAC TTAGAGATCC TCAAGGCTGA GATTGAAGAG AAGGGCTCAG 
     1681 ATGGCGCTGC ATCCAGTTAT CAGCTCAAGC AGCTTGAGGA GCAGAATGCC CGCCTGAAGG 
     1741 ATGCCCTGGT GAGGATGCGG GATCTTTCTT CCTCAGAGAA GCAGGAGCAT GTGAAGCTCC 
     1801 AGAAGCTCAT GGAAAAGAAG AACCAAGAGC TGGAAGTTGT GAGGCAACAG CGGGAGCGTC 
     1861 TGCAGGAGGA GCTAAGCCAG GCAGAGAGCA CCATTGATGA GCTCAAGGAG CAGGTGGATG 
     1921 CTGCTCTGGG TGCTGAGGAG ATGGTGGAGA TGCTGACAGA TCGGAACCTG AATCTGGAAG 
     1981 AGAAAGTGCG CGAGTTGAGG GAGACTGTGG GAGACTTGGA AGCGATGAAT GAGATGAACG 
     2041 ATGAGCTGCA GGAGAATGCA CGTGAGACAG AACTGGAGCT GCGGGAGCAG CTGGACATGG 
     2101 CAGGCGCGCG GGTTCGTGAG GCCCAGAAGC GTGTGGAGGC AGCCCAGGAG ACGGTTGCAG 
     2161 ACTACCAGCA GACCATCAAG AAGTACCGCC AGCTGACCGC CCATCTACAG GATGTGAATC 
     2221 GGGAACTGAC AAACCAGCAG GAAGCATCTG TGGAGAGGCA ACAGCAGCCA CCTCCAGAGA 
     2281 CCTTTGACTT CAAAATCAAG TTTGCTGAGA CTAAGGCCCA TGCCAAGGCA ATTGAGATGG 
     2341 AATTGAGGCA GATGGAGGTG GCCCAGGCCA ATCGACACAT GTCCCTGCTG ACAGCCTTCA 
     2401 TGCCTGACAG CTTCCTTCGG CCAGGTGGGG ACCATGACTG CGTTCTGGTG CTGTTGCTCA 
     2461 TGCCTCGTCT CATTTGCAAG GCAGAGCTGA TCCGGAAGCA GGCCCAGGAG AAGTTTGAAC 
     2521 TAAGTGAGAA CTGTTCAGAG CGGCCTGGGC TGCGAGGAGC TGCTGGGGAG CAACTCAGCT 
     2581 TTGCTGCTGG ACTGGTGTAC TCGCTGAGCC TGCTGCAGGC CACGCTACAC CGCTATGAGC 
     2641 ATGCCCTCTC TCAGTGCAGT GTGGATGTGT ATAAGAAAGT GGGCAGCCTG TACCCTGAGA 
     2701 TGAGTGCCCA TGAGCGCTCC TTGGATTTCC TCATTGAACT GCTGCACAAG GATCAGCTGG 
     2761 ATGAGACTGT CAATGTGGAG CCTCTCACCA AGGCCATCAA GTACTATCAG CATCTGTACA 
     2821 GCATCCACCT TGCCGAACAG CCTGAGGACT GTACTATGCA GCTGGCTGAC CACATTAAGT 
     2881 TCACGCAGAG TGCTCTGGAC TGCATGAGTG TGGAGGTAGG ACGGCTGCGT GCCTTCTTGC 
     2941 AGGGTGGGCA GGAGGCTACA GATATTGCCC TCCTGCTCCG GGATCTGGAA ACTTCATGCA 
     3001 GTGACATCCG CCAGTTCTGC AAGAAGATCC GAAGGCGAAT GCCAGGGACA GATGCTCCTG 
     3061 GGATCCCAGC TGCACTGGCC TTTGGACCAC AGGTATCTGA CACGCTCCTA GACTGCAGGA 
     3121 AACACTTGAC GTGGGTCGTG GCTGTGCTGC AGGAGGTGGC AGCTGCTGCT GCCCAGCTCA 
     3181 TTGCCCCACT GGCAGAGAAT GAGGGGCTAC TTGTGGCTGC TCTGGAGGAA CTGGCTTTCA 
     3241 AAGCAAGCGA GCAGATCTAT GGGACCCCCT CCAGCAGCCC CTATGAGTGT CTGCGCCAGT 
     3301 CATGCAACAT CCTCATCAGT ACCATGAACA AGCTGGCCAC AGCCATGCAG GAGGGGGAGT 
     3361 ATGATGCAGA GCGGCCCCCC AGCAAGCCTC CACCGGTTGA ACTGCGGGCT GCTGCCCTTC 
     3421 GTGCAGAGAT CACAGATGCT GAAGGCCTGG GTTTGAAGCT CGAAGATCGA GAGACAGTTA 
     3481 TTAAGGAGTT GAAGAAGTCA CTCAAGATTA AGGGAGAGGA GCTAAGTGAG GCCAATGTGC 
     3541 GGCTGAGCCT CCTGGAGAAG AAGTTGGACA GTGCTGCCAA GGATGCAGAT GAGCGCATCG 
     3601 AGAAAGTCCA GACTCGGCTG GAGGAGACCC AGGCACTGCT GCGAAAGAAG GAGAAAGAGT 
     3661 TTGAGGAGAC AATGGATGCA CTCCAGGCTG ACATCGACCA GCTGGAGGCA GAGAAGGCAG 
     3721 AACTAAAGCA GCGTCTGAAC AGCCAGTCCA AACGCACGAT TGAGGGACTC CGGGGCCCTC 
     3781 CTCCTTCAGG CATTGCTACT CTGGTCTCTG GCATTGCTGG TGAAGAACAG CAGCGAGGAG 
     3841 CCATCCCTGG GCAGGCTCCA GGGTCTGTGC CAGGCCCAGG GCTGGTGAAG GACTCACCAC 
     3901 TGCTGCTTCA GCAGATCTCT GCCATGAGGC TGCACATCTC CCAGCTCCAG CATGAGAACA 
     3961 GCATCCTCAA GGGAGCCCAG ATGAAGGCAT CCTTGGCATC CCTGCCCCCT CTGCATGTTG 
     4021 CAAAGCTATC CCATGAGGGC CCTGGCAGTG AGTTACCAGC TGGAGCGCTG TATCGTAAGA 
     4081 CCAGCCAGCT GCTGGAGACA TTGAATCAAT TGAGCACACA CACGCACGTA GTAGACATCA 
     4141 CTCGCACCAG CCCTGCTGCC AAGAGCCCGT CGGCCCAACT TATGGAGCAA GTGGCTCAGC 
     4201 TTAAGTCCCT GAGTGACACC GTCGAGAAGC TCAAGGATGA GGTCCTCAAG GAGACAGTAT 
     4261 CTCAGCGCCC TGGAGCCACA GTACCCACTG ACTTTGCCAC CTTCCCTTCA TCAGCCTTCC 
     4321 TCAGGGCCAA GGAGGAGCAG CAGGATGACA CAGTCTACAT GGGCAAAGTG ACCTTCTCAT 
     4381 GTGCGGCTGG TTTTGGACAG CGACACCGGC TGGTGCTGAC CCAGGAGCAG CTGCACCAGC 
     4441 TTCACAGTCG CCTCATCTCC ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     4501 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     4561 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     4621 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     4681 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     4741 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     4801 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     4861 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     4921 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     4981 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     5041 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     5101 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     5161 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     5221 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     5281 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     5341 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     5401 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     5461 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     5521 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     5581 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     5641 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     5701 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     5761 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     5821 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     5881 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     5941 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     6001 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     6061 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     6121 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     6181 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     6241 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     6301 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     6361 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     6421 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     6481 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     6541 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     6601 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     6661 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     6721 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     6781 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     6841 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     6901 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     6961 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     7021 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     7081 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     7141 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     7201 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     7261 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     7321 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     7381 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     7441 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     7501 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     7561 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7621 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7681 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     7741 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     7801 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     7861 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     7921 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     7981 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     8041 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     8101 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     8161 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     8221 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     8281 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//