LOCUS       RC223535                9234 bp    DNA     circular  SYN 
DEFINITION  FANCD2 (Myc-DDK-tagged)-Human Fanconi anemia, complementation group D2 (FANCD2), transcript variant 2
ACCESSION   NM_001018115
VERSION     NM_001018115.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5391..5482 
                     /label="Myc-DDK"  
     misc_feature    5512..6097 
                     /label="PolyA signal"  
     misc_feature    5569..5588 
                     /label="Restriction Enzyme"  
     misc_feature    6373..7045 
                     /label="ColE1"  
     misc_feature    7475..8269 
                     /label="Kan/Neo"  
     misc_feature    8304..8606 
                     /label="SV40 ori"  
     misc_feature    8692..9169 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5373..5379 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5373 
                     /label="FANCD2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTTTCCAAA AGAAGACTGT CAAAATCTGA GGATAAAGAG AGCCTGACAG 
     1081 AAGATGCCTC CAAAACCAGG AAGCAACCAC TTTCCAAAAA GACAAAGAAA TCTCATATTG 
     1141 CTAATGAAGT TGAAGAAAAT GACAGCATCT TTGTAAAGCT TCTTAAGATA TCAGGAATTA 
     1201 TTCTTAAAAC GGGAGAGAGT CAGAATCAAC TAGCTGTGGA TCAAATAGCT TTCCAAAAGA 
     1261 AGCTCTTTCA GACCCTGAGG AGACACCCTT CCTATCCCAA AATAATAGAA GAATTTGTTA 
     1321 GTGGCCTGGA GTCTTACATT GAGGATGAAG ACAGTTTCAG GAACTGCCTT TTGTCTTGTG 
     1381 AGCGTCTGCA GGATGAGGAA GCCAGTATGG GTGCATCTTA TTCTAAGAGT CTCATCAAAC 
     1441 TGCTTCTGGG GATTGACATA CTGCAGCCTG CCATTATCAA AACCTTATTT GAGAAGTTGC 
     1501 CAGAATATTT TTTTGAAAAC AAGAACAGTG ATGAAATCAA CATACCTCGA CTCATTGTCA 
     1561 GTCAACTAAA ATGGCTTGAC AGAGTTGTGG ATGGCAAGGA CCTCACCACC AAGATCATGC 
     1621 AGCTGATCAG TATTGCTCCA GAGAACCTGC AGCATGACAT CATCACCAGC CTACCTGAGA 
     1681 TCCTAGGGGA TTCCCAGCAC GCTGATGTGG GGAAAGAACT CAGTGACCTA CTGATAGAGA 
     1741 ATACTTCACT CACTGTCCCA ATCCTGGATG TCCTTTCAAG CCTCCGACTT GACCCAAACT 
     1801 TCCTATTGAA GGTTCGCCAG TTGGTGATGG ATAAGTTGTC GTCTATTAGA TTGGAGGATT 
     1861 TACCTGTGAT AATAAAGTTC ATTCTTCATT CCGTAACAGC CATGGATACA CTTGAGGTAA 
     1921 TTTCTGAGCT TCGGGAGAAG TTGGATCTGC AGCATTGTGT TTTGCCATCA CGGTTACAGG 
     1981 CTTCCCAAGT AAAGTTGAAA AGTAAAGGAC GAGCAAGTTC CTCAGGAAAT CAAGAAAGCA 
     2041 GCGGTCAGAG CTGTATTATT CTCCTCTTTG ATGTAATAAA GTCAGCTATT AGATATGAGA 
     2101 AAACCATTTC AGAAGCCTGG ATTAAGGCAA TTGAAAACAC TGCCTCAGTA TCTGAACACA 
     2161 AGGTGTTTGA CCTGGTGATG CTTTTCATCA TCTATAGCAC CAATACTCAG ACAAAGAAGT 
     2221 ACATTGACAG GGTGCTAAGA AATAAGATTC GATCAGGCTG CATTCAAGAA CAGCTGCTCC 
     2281 AGAGTACATT CTCTGTTCAT TACTTAGTTC TTAAGGATAT GTGTTCATCC ATTCTGTCGC 
     2341 TGGCTCAGAG TTTGCTTCAC TCTCTAGACC AGAGTATAAT TTCATTTGGC AGTCTCCTAT 
     2401 ACAAATATGC ATTTAAGTTT TTTGACACGT ACTGCCAGCA GGAAGTGGTT GGTGCCTTAG 
     2461 TGACCCATAT CTGCAGTGGG AATGAAGCTG AAGTTGATAC TGCCTTAGAT GTCCTTCTAG 
     2521 AGTTGGTAGT GTTAAACCCA TCTGCTATGA TGATGAATGC TGTCTTTGTA AAGGGCATTT 
     2581 TAGATTATCT GGATAACATA TCCCCTCAGC AAATACGAAA ACTCTTCTAT GTTCTCAGCA 
     2641 CACTGGCATT TAGCAAACAG AATGAAGCCA GCAGCCACAT CCAGGATGAC ATGCACTTGG 
     2701 TGATAAGAAA GCAGCTCTCT AGCACCGTAT TCAAGTACAA GCTCATTGGG ATTATTGGTG 
     2761 CTGTGACCAT GGCTGGCATC ATGGCGGCAG ACAGAAGTGA ATCACCTAGT TTGACCCAAG 
     2821 AGAGAGCCAA CCTGAGCGAT GAGCAGTGCA CACAGGTGAC CTCCTTGTTG CAGTTGGTTC 
     2881 ATTCCTGCAG TGAGCAGTCT CCTCAGGCCT CTGCACTTTA CTATGATGAA TTTGCCAACC 
     2941 TGATCCAACA TGAAAAGCTG GATCCAAAAG CCCTGGAATG GGTTGGGCAT ACCATCTGTA 
     3001 ATGATTTCCA GGATGCCTTC GTAGTGGACT CCTGTGTTGT TCCGGAAGGT GACTTTCCAT 
     3061 TTCCTGTGAA AGCACTGTAC GGACTGGAAG AATACGACAC TCAGGATGGG ATTGCCATAA 
     3121 ACCTCCTGCC GCTGCTGTTT TCTCAGGACT TTGCAAAAGA TGGGGGTCCG GTGACCTCAC 
     3181 AGGAATCAGG CCAAAAATTG GTGTCTCCGC TGTGCCTGGC TCCGTATTTC CGGTTACTGA 
     3241 GACTTTGTGT GGAGAGACAG CATAACGGAA ACTTGGAGGA GATTGATGGT CTACTAGATT 
     3301 GTCCTATATT CCTAACTGAC CTGGAGCCTG GAGAGAAGTT GGAGTCCATG TCTGCTAAAG 
     3361 AGCGTTCATT CATGTGTTCT CTCATATTTC TTACTCTCAA CTGGTTCCGA GAGATTGTAA 
     3421 ATGCCTTCTG CCAGGAAACA TCACCTGAGA TGAAGGGGAA GGTGCTCACT CGGTTAAAGC 
     3481 ACATTGTAGA ATTGCAAATA ATCCTGGAAA AGTACTTGGC AGTCACCCCA GACTATGTCC 
     3541 CTCCTCTTGG AAACTTTGAT GTGGAAACTT TAGATATAAC ACCTCATACT GTTACTGCTA 
     3601 TTTCAGCAAA AATCAGAAAG AAAGGAAAAA TAGAAAGGAA ACAAAAAACA GATGGCAGCA 
     3661 AGACATCCTC CTCTGACACA CTTTCAGAAG AGAAAAATTC AGAATGTGAC CCTACGCCAT 
     3721 CTCATAGAGG CCAGCTAAAC AAGGAGTTCA CAGGGAAGGA AGAAAAGACA TCATTGTTAC 
     3781 TACATAATTC CCATGCTTTT TTCCGAGAGC TGGACATTGA GGTCTTCTCT ATTCTACATT 
     3841 GTGGACTTGT GACGAAGTTC ATCTTAGATA CTGAAATGCA CACTGAAGCT ACAGAAGTTG 
     3901 TGCAACTTGG GCCCCCTGAG CTGCTTTTCT TGCTGGAAGA TCTCTCCCAG AAGCTGGAGA 
     3961 GTATGCTGAC ACCTCCTATT GCCAGGAGAG TCCCCTTTCT CAAGAACAAA GGAAGCCGGA 
     4021 ATATTGGATT CTCACATCTC CAACAGAGAT CTGCCCAAGA AATTGTTCAT TGTGTTTTTC 
     4081 AACTGCTGAC CCCAATGTGT AACCACCTGG AGAACATTCA CAACTATTTT CAGTGTTTAG 
     4141 CTGCTGAGAA TCACGGTGTA GTTGATGGAC CAGGAGTGAA AGTTCAGGAG TACCACATAA 
     4201 TGTCTTCCTG CTATCAGAGG CTGCTGCAGA TTTTTCATGG GCTTTTTGCT TGGAGTGGAT 
     4261 TTTCTCAACC TGAAAATCAG AATTTACTGT ATTCAGCCCT CCATGTCCTT AGTAGCCGAC 
     4321 TGAAACAGGG AGAACACAGC CAGCCTTTGG AGGAACTACT CAGCCAGAGC GTCCATTACT 
     4381 TGCAGAATTT CCATCAAAGC ATTCCCAGTT TCCAGTGTGC TCTTTATCTC ATCAGACTTT 
     4441 TGATGGTTAT TTTGGAGAAA TCAACAGCTT CTGCTCAGAA CAAAGAAAAA ATTGCTTCCC 
     4501 TTGCCAGACA ATTCCTCTGT CGGGTGTGGC CAAGTGGGGA TAAAGAGAAG AGCAACATCT 
     4561 CTAATGACCA GCTCCATGCT CTGCTCTGTA TCTACCTGGA GCACACAGAG AGCATTCTGA 
     4621 AGGCCATAGA GGAGATTGCT GGTGTTGGTG TCCCAGAACT GATCAACTCT CCTAAAGATG 
     4681 CATCTTCCTC CACATTCCCT ACACTGACCA GGCATACTTT TGTTGTTTTC TTCCGTGTGA 
     4741 TGATGGCTGA ACTAGAGAAG ACGGTGAAAA AAATTGAGCC TGGCACAGCA GCAGACTCGC 
     4801 AGCAGATTCA TGAAGAGAAA CTCCTCTACT GGAACATGGC TGTTCGAGAC TTCAGTATCC 
     4861 TCATCAACTT GATAAAGGTA TTTGATAGTC ATCCTGTTCT GCATGTATGT TTGAAGTATG 
     4921 GGCGTCTCTT TGTGGAAGCA TTTCTGAAGC AATGTATGCC GCTCCTAGAC TTCAGTTTTA 
     4981 GAAAACACCG GGAAGATGTT CTGAGCTTAC TGGAAACCTT CCAGTTGGAC ACAAGGCTGC 
     5041 TTCATCACCT GTGTGGGCAT TCCAAGATTC ACCAGGACAC GAGACTCACC CAACATGTGC 
     5101 CTCTGCTCAA AAAGACCCTG GAACTTTTAG TTTGCAGAGT CAAAGCTATG CTCACTCTCA 
     5161 ACAATTGTAG AGAGGCTTTC TGGCTGGGCA ATCTAAAAAA CCGGGACTTG CAGGGTGAAG 
     5221 AGATTAAGTC CCAAAATTCC CAGGAGAGCA CAGCAGATGA GAGTGAGGAT GACATGTCAT 
     5281 CCCAGGCCTC CAAGAGCAAA GCCACTGAGG ATGGTGAAGA AGACGAAGTA AGTGCTGGAG 
     5341 AAAAGGAGCA AGATAGTGAT GAGAGTTATG ATGACTCTGA TACGCGTACG CGGCCGCTCG 
     5401 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     5461 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     5521 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     5581 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     5641 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     5701 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     5761 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     5821 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     5881 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     5941 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     6001 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     6061 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     6121 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     6181 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     6241 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     6301 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     6361 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     6421 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     6481 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     6541 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     6601 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     6661 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     6721 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     6781 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     6841 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     6901 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     6961 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     7021 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     7081 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     7141 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     7201 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     7261 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     7321 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     7381 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     7441 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     7501 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     7561 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     7621 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     7681 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     7741 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     7801 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     7861 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     7921 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     7981 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     8041 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     8101 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     8161 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     8221 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     8281 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     8341 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     8401 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     8461 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     8521 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     8581 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     8641 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     8701 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     8761 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     8821 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     8881 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     8941 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     9001 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     9061 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     9121 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     9181 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//