LOCUS       RC223526                7926 bp    DNA     circular  SYN 
DEFINITION  EPHA5 (Myc-DDK-tagged)-Human EPH receptor A5 (EPHA5), transcript variant 2
ACCESSION   NM_182472
VERSION     NM_182472.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4083..4174 
                     /label="Myc-DDK"  
     misc_feature    4204..4789 
                     /label="PolyA signal"  
     misc_feature    4261..4280 
                     /label="Restriction Enzyme"  
     misc_feature    5065..5737 
                     /label="ColE1"  
     misc_feature    6167..6961 
                     /label="Kan/Neo"  
     misc_feature    6996..7298 
                     /label="SV40 ori"  
     misc_feature    7384..7861 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4065..4071 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4065 
                     /label="EPHA5"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCGGGGCTCG GGGCCCCGGG GTGCGGGACG CCGGCGGCCC CCAAGCGGCG 
     1081 GCGGCGACAC CCCCATCACC CCAGCGTCCC TGGCCGGCTG CTACTCTGCA CCTCGACGGG 
     1141 CTCCCCTCTG GACGTGCCTT CTCCTGTGCG CCGCACTCCG GACCCTCCTG GCCAGCCCCA 
     1201 GCAACGAAGT GAATTTATTG GATTCACGCA CTGTCATGGG GGACCTGGGA TGGATTGCTT 
     1261 TTCCAAAAAA TGGGTGGGAA GAGATTGGTG AAGTGGATGA AAATTATGCC CCTATCCACA 
     1321 CATACCAAGT ATGCAAAGTG ATGGAACAGA ATCAGAATAA CTGGCTTTTG ACCAGTTGGA 
     1381 TCTCCAATGA AGGTGCTTCC AGAATCTTCA TAGAACTCAA ATTTACCCTG CGGGACTGCA 
     1441 ACAGCCTTCC TGGAGGACTG GGGACCTGTA AGGAAACCTT TAATATGTAT TACTTTGAGT 
     1501 CAGATGATCA GAATGGGAGA AACATCAAGG AAAACCAATA CATCAAAATT GATACCATTG 
     1561 CTGCCGATGA AAGCTTTACA GAACTTGATC TTGGTGACCG TGTTATGAAA CTGAATACAG 
     1621 AGGTCAGAGA TGTAGGACCT CTAAGCAAAA AGGGATTTTA TCTTGCTTTT CAAGATGTTG 
     1681 GTGCTTGCAT TGCTCTGGTT TCTGTGCGTG TATACTATAA AAAATGCCCT TCTGTGGTAC 
     1741 GACACTTGGC TGTCTTCCCT GACACCATCA CTGGAGCTGA TTCTTCCCAA TTGCTCGAAG 
     1801 TGTCAGGCTC CTGTGTCAAC CATTCTGTGA CCGATGAACC TCCCAAAATG CACTGCAGCG 
     1861 CCGAAGGGGA GTGGCTGGTG CCCATCGGGA AATGCATGTG CAAGGCAGGA TATGAAGAGA 
     1921 AAAATGGCAC CTGTCAAGTG TGCAGACCTG GGTTCTTCAA AGCCTCACCT CACATCCAGA 
     1981 GCTGCGGCAA ATGTCCACCT CACAGTTATA CCCATGAGGA AGCTTCAACC TCTTGTGTCT 
     2041 GTGAAAAGGA TTATTTCAGG AGAGAGTCTG ATCCACCCAC AATGGCATGC ACAAGACCCC 
     2101 CCTCTGCTCC TCGGAATGCC ATCTCAAATG TTAATGAAAC TAGTGTCTTT CTGGAATGGA 
     2161 TTCCGCCTGC TGACACTGGT GGAAGGAAAG ACGTGTCATA TTATATTGCA TGCAAGAAGT 
     2221 GCAACTCCCA TGCAGGTGTG TGTGAGGAGT GTGGCGGTCA TGTCAGGTAC CTTCCCCGGC 
     2281 AAAGCGGCCT GAAAAACACC TCTGTCATGA TGGTGGATCT ACTCGCTCAC ACAAACTATA 
     2341 CCTTTGAGAT TGAGGCAGTG AATGGAGTGT CCGACTTGAG CCCAGGAGCC CGGCAGTATG 
     2401 TGTCTGTAAA TGTAACCACA AATCAAGCAG CTCCATCTCC AGTCACCAAT GTGAAAAAAG 
     2461 GGAAAATTGC AAAAAACAGC ATCTCTTTGT CTTGGCAAGA ACCAGATCGT CCCAATGGAA 
     2521 TCATCCTAGA GTATGAAATC AAGTATTTTG AAAAGGACCA AGAGACCAGC TACACGATTA 
     2581 TCAAATCTAA AGAGACAACT ATTACTGCAG AGGGCTTGAA ACCAGCTTCA GTTTATGTCT 
     2641 TCCAAATTCG AGCACGTACA GCAGCAGGCT ATGGTGTCTT CAGTCGAAGA TTTGAGTTTG 
     2701 AAACCACCCC AGTGTTTGCA GCATCCAGCG ATCAAAGCCA GATTCCTGTA ATTGCTGTGT 
     2761 CTGTGACAGT GGGAGTCATT TTGTTGGCAG TGGTTATCGG CGTCCTCCTC AGTGGAAGGC 
     2821 GGTGTGGCTA CAGCAAAGCA AAACAAGATC CAGAAGAGGA AAAGATGCAT TTTCATAATG 
     2881 GGCACATTAA ACTGCCAGGA GTAAGAACTT ACATTGATCC ACATACCTAT GAGGATCCCA 
     2941 ATCAAGCTGT CCACGAATTT GCTAAGGAGA TAGAAGCATC ATGTATCACC ATTGAGAGAG 
     3001 TTATTGGAGC AGGTGAATTT GGTGAAGTTT GTAGTGGACG TTTGAAACTA CCAGGAAAAA 
     3061 GAGAATTACC TGTGGCTATC AAAACCCTTA AAGTAGGCTA TACTGAAAAG CAACGCAGAG 
     3121 ATTTCCTAGG TGAAGCAAGT ATCATGGGAC AGTTTGATCA TCCTAACATC ATCCATTTAG 
     3181 AAGGTGTGGT GACCAAAAGT AAACCAGTGA TGATCGTGAC AGAGTATATG GAGAATGGCT 
     3241 CTTTAGATAC ATTTTTGAAG AAAAACGATG GGCAGTTCAC TGTGATTCAG CTTGTTGGCA 
     3301 TGCTGAGAGG TATCTCTGCA GGAATGAAGT ACCTTTCTGA CATGGGCTAT GTGCATAGAG 
     3361 ATCTTGCTGC CAGAAACATC TTAATCAACA GTAACCTTGT GTGCAAAGTG TCTGACTTTG 
     3421 GACTTTCCCG GGTACTGGAA GATGATCCCG AGGCAGCCTA CACCACAAGG GGAGGAAAAA 
     3481 TTCCAATCAG ATGGACTGCC CCAGAAGCAA TAGCTTTCCG AAAGTTTACT TCTGCCAGTG 
     3541 ATGTCTGGAG TTATGGAATA GTAATGTGGG AAGTTGTGTC TTATGGAGAG AGACCCTACT 
     3601 GGGAGATGAC CAATCAAGAT GTGATTAAAG CGGTAGAGGA AGGCTATCGT CTGCCAAGCC 
     3661 CCATGGATTG TCCTGCTGCT CTCTATCAGT TAATGCTGGA TTGCTGGCAG AAAGAGCGAA 
     3721 ATAGCAGGCC CAAGTTTGAT GAAATAGTCA ACATGTTGGA CAAGCTGATA CGTAACCCAA 
     3781 GTAGTCTGAA GACGCTGGTT AATGCATCCT GCAGAGTATC TAATTTATTG GCAGAACATA 
     3841 GCCCACTAGG ATCTGGGGCC TACAGATCAG TAGGTGAATG GCTAGAGGCA ATCAAGATGG 
     3901 GCCGGTATAC AGAGATTTTC ATGGAAAATG GATACAGTTC AATGGACGCT GTGGCTCAGG 
     3961 TGACCTTGGA GGATTTGAGA CGGCTTGGAG TGACTCTTGT CGGTCACCAG AAGAAGATCA 
     4021 TGAACAGCCT TCAAGAAATG AAGGTGCAGC TGGTAAACGG AATGGTGCCA TTGACGCGTA 
     4081 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     4141 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     4201 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     4261 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     4321 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     4381 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     4441 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     4501 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     4561 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     4621 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     4681 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     4741 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     4801 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     4861 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     4921 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     4981 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     5041 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     5101 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     5161 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     5221 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     5281 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     5341 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     5401 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     5461 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     5521 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     5581 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     5641 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     5701 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     5761 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     5821 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     5881 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     5941 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     6001 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     6061 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     6121 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     6181 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     6241 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     6301 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     6361 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     6421 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     6481 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     6541 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     6601 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     6661 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     6721 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     6781 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     6841 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     6901 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     6961 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     7021 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     7081 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     7141 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     7201 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     7261 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7321 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     7381 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     7441 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     7501 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     7561 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     7621 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     7681 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     7741 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     7801 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     7861 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     7921 AATTTT
//