LOCUS       RC223470                8457 bp    DNA     circular  SYN 
DEFINITION  RTN4 (Myc-DDK-tagged)-Human reticulon 4 (RTN4), transcript variant 1
ACCESSION   NM_020532
VERSION     NM_020532.4
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4614..4705 
                     /label="Myc-DDK"  
     misc_feature    4735..5320 
                     /label="PolyA signal"  
     misc_feature    4792..4811 
                     /label="Restriction Enzyme"  
     misc_feature    5596..6268 
                     /label="ColE1"  
     misc_feature    6698..7492 
                     /label="Kan/Neo"  
     misc_feature    7527..7829 
                     /label="SV40 ori"  
     misc_feature    7915..8392 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4596..4602 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4596 
                     /label="RTN4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAAGACCTG GACCAGTCTC CTCTGGTCTC GTCCTCGGAC AGCCCACCCC 
     1081 GGCCGCAGCC CGCGTTCAAG TACCAGTTCG TGAGGGAGCC CGAGGACGAG GAGGAAGAAG 
     1141 AGGAGGAGGA AGAGGAGGAC GAGGACGAAG ACCTGGAGGA GCTGGAGGTG CTGGAGAGGA 
     1201 AGCCCGCCGC CGGGCTGTCC GCGGCCCCAG TGCCCACCGC CCCTGCCGCC GGCGCGCCCC 
     1261 TGATGGACTT CGGAAATGAC TTCGTGCCGC CGGCGCCCCG GGGACCCCTG CCGGCCGCTC 
     1321 CCCCCGTCGC CCCGGAGCGG CAGCCGTCTT GGGACCCGAG CCCGGTGTCG TCGACCGTGC 
     1381 CCGCGCCATC CCCGCTGTCT GCTGCCGCAG TCTCGCCCTC CAAGCTCCCT GAGGACGACG 
     1441 AGCCTCCGGC CCGGCCTCCC CCTCCTCCCC CGGCCAGCGT GAGCCCCCAG GCAGAGCCCG 
     1501 TGTGGACCCC GCCAGCCCCG GCTCCCGCCG CGCCCCCCTC CACCCCGGCC GCGCCCAAGC 
     1561 GCAGGGGCTC CTCGGGCTCA GTGGATGAGA CCCTTTTTGC TCTTCCTGCT GCATCTGAGC 
     1621 CTGTGATACG CTCCTCTGCA GAAAATATGG ACTTGAAGGA GCAGCCAGGT AACACTATTT 
     1681 CGGCTGGTCA AGAGGATTTC CCATCTGTCC TGCTTGAAAC TGCTGCTTCT CTTCCTTCTC 
     1741 TGTCTCCTCT CTCAGCCGCT TCTTTCAAAG AACATGAATA CCTTGGTAAT TTGTCAACAG 
     1801 TATTACCCAC TGAAGGAACA CTTCAAGAAA ATGTCAGTGA AGCTTCTAAA GAGGTCTCAG 
     1861 AGAAGGCAAA AACTCTACTC ATAGATAGAG ATTTAACAGA GTTTTCAGAA TTAGAATACT 
     1921 CAGAAATGGG ATCATCGTTC AGTGTCTCTC CAAAAGCAGA ATCTGCCGTA ATAGTAGCAA 
     1981 ATCCTAGGGA AGAAATAATC GTGAAAAATA AAGATGAAGA AGAGAAGTTA GTTAGTAATA 
     2041 ACATCCTTCA TAATCAACAA GAGTTACCTA CAGCTCTTAC TAAATTGGTT AAAGAGGATG 
     2101 AAGTTGTGTC TTCAGAAAAA GCAAAAGACA GTTTTAATGA AAAGAGAGTT GCAGTGGAAG 
     2161 CTCCTATGAG GGAGGAATAT GCAGACTTCA AACCATTTGA GCGAGTATGG GAAGTGAAAG 
     2221 ATAGTAAGGA AGATAGTGAT ATGTTGGCTG CTGGAGGTAA AATCGAGAGC AACTTGGAAA 
     2281 GTAAAGTGGA TAAAAAATGT TTTGCAGATA GCCTTGAGCA AACTAATCAC GAAAAAGATA 
     2341 GTGAGAGTAG TAATGATGAT ACTTCTTTCC CCAGTACGCC AGAAGGTATA AAGGATCGTT 
     2401 CAGGAGCATA TATCACATGT GCTCCCTTTA ACCCAGCAGC AACTGAGAGC ATTGCAACAA 
     2461 ACATTTTTCC TTTGTTAGGA GATCCTACTT CAGAAAATAA GACCGATGAA AAAAAAATAG 
     2521 AAGAAAAGAA GGCCCAAATA GTAACAGAGA AGAATACTAG CACCAAAACA TCAAACCCTT 
     2581 TTCTTGTAGC AGCACAGGAT TCTGAGACAG ATTATGTCAC AACAGATAAT TTAACAAAGG 
     2641 TGACTGAGGA AGTCGTGGCA AACATGCCTG AAGGCCTGAC TCCAGATTTA GTACAGGAAG 
     2701 CATGTGAAAG TGAATTGAAT GAAGTTACTG GTACAAAGAT TGCTTATGAA ACAAAAATGG 
     2761 ACTTGGTTCA AACATCAGAA GTTATGCAAG AGTCACTCTA TCCTGCAGCA CAGCTTTGCC 
     2821 CATCATTTGA AGAGTCAGAA GCTACTCCTT CACCAGTTTT GCCTGACATT GTTATGGAAG 
     2881 CACCATTGAA TTCTGCAGTT CCTAGTGCTG GTGCTTCCGT GATACAGCCC AGCTCATCAC 
     2941 CATTAGAAGC TTCTTCAGTT AATTATGAAA GCATAAAACA TGAGCCTGAA AACCCCCCAC 
     3001 CATATGAAGA GGCCATGAGT GTATCACTAA AAAAAGTATC AGGAATAAAG GAAGAAATTA 
     3061 AAGAGCCTGA AAATATTAAT GCAGCTCTTC AAGAAACAGA AGCTCCTTAT ATATCTATTG 
     3121 CATGTGATTT AATTAAAGAA ACAAAGCTTT CTGCTGAACC AGCTCCGGAT TTCTCTGATT 
     3181 ATTCAGAAAT GGCAAAAGTT GAACAGCCAG TGCCTGATCA TTCTGAGCTA GTTGAAGATT 
     3241 CCTCACCTGA TTCTGAACCA GTTGACTTAT TTAGTGATGA TTCAATACCT GACGTTCCAC 
     3301 AAAAACAAGA TGAAACTGTG ATGCTTGTGA AAGAAAGTCT CACTGAGACT TCATTTGAGT 
     3361 CAATGATAGA ATATGAAAAT AAGGAAAAAC TCAGTGCTTT GCCACCTGAG GGAGGAAAGC 
     3421 CATATTTGGA ATCTTTTAAG CTCAGTTTAG ATAACACAAA AGATACCCTG TTACCTGATG 
     3481 AAGTTTCAAC ATTGAGCAAA AAGGAGAAAA TTCCTTTGCA GATGGAGGAG CTCAGTACTG 
     3541 CAGTTTATTC AAATGATGAC TTATTTATTT CTAAGGAAGC ACAGATAAGA GAAACTGAAA 
     3601 CGTTTTCAGA TTCATCTCCA ATTGAAATTA TAGATGAGTT CCCTACATTG ATCAGTTCTA 
     3661 AAACTGATTC ATTTTCTAAA TTAGCCAGGG AATATACTGA CCTAGAAGTA TCCCACAAAA 
     3721 GTGAAATTGC TAATGCCCCG GATGGAGCTG GGTCATTGCC TTGCACAGAA TTGCCCCATG 
     3781 ACCTTTCTTT GAAGAACATA CAACCCAAAG TTGAAGAGAA AATCAGTTTC TCAGATGACT 
     3841 TTTCTAAAAA TGGGTCTGCT ACATCAAAGG TGCTCTTATT GCCTCCAGAT GTTTCTGCTT 
     3901 TGGCCACTCA AGCAGAGATA GAGAGCATAG TTAAACCCAA AGTTCTTGTG AAAGAAGCTG 
     3961 AGAAAAAACT TCCTTCCGAT ACAGAAAAAG AGGACAGATC ACCATCTGCT ATATTTTCAG 
     4021 CAGAGCTGAG TAAAACTTCA GTTGTTGACC TCCTGTACTG GAGAGACATT AAGAAGACTG 
     4081 GAGTGGTGTT TGGTGCCAGC CTATTCCTGC TGCTTTCATT GACAGTATTC AGCATTGTGA 
     4141 GCGTAACAGC CTACATTGCC TTGGCCCTGC TCTCTGTGAC CATCAGCTTT AGGATATACA 
     4201 AGGGTGTGAT CCAAGCTATC CAGAAATCAG ATGAAGGCCA CCCATTCAGG GCATATCTGG 
     4261 AATCTGAAGT TGCTATATCT GAGGAGTTGG TTCAGAAGTA CAGTAATTCT GCTCTTGGTC 
     4321 ATGTGAACTG CACGATAAAG GAACTCAGGC GCCTCTTCTT AGTTGATGAT TTAGTTGATT 
     4381 CTCTGAAGTT TGCAGTGTTG ATGTGGGTAT TTACCTATGT TGGTGCCTTG TTTAATGGTC 
     4441 TGACACTACT GATTTTGGCT CTCATTTCAC TCTTCAGTGT TCCTGTTATT TATGAACGGC 
     4501 ATCAGGCACA GATAGATCAT TATCTAGGAC TTGCAAATAA GAATGTTAAA GATGCTATGG 
     4561 CTAAAATCCA AGCAAAAATC CCTGGATTGA AGCGCAAAGC TGAAACGCGT ACGCGGCCGC 
     4621 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     4681 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     4741 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     4801 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     4861 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     4921 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     4981 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     5041 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     5101 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     5161 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     5221 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     5281 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     5341 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     5401 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     5461 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     5521 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     5581 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     5641 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     5701 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     5761 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     5821 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     5881 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     5941 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     6001 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     6061 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     6121 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     6181 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     6241 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     6301 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     6361 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     6421 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     6481 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     6541 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     6601 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     6661 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     6721 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     6781 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     6841 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     6901 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     6961 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     7021 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     7081 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     7141 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     7201 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     7261 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     7321 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     7381 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     7441 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     7501 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     7561 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     7621 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     7681 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7741 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     7801 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     7861 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     7921 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     7981 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     8041 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     8101 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     8161 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     8221 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     8281 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     8341 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     8401 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//