LOCUS       RC223406                8670 bp    DNA     circular  SYN 
DEFINITION  SIK3 (Myc-DDK-tagged)-Human SIK family kinase 3 (SIK3)
ACCESSION   NM_025164
VERSION     NM_025164.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4827..4918 
                     /label="Myc-DDK"  
     misc_feature    4948..5533 
                     /label="PolyA signal"  
     misc_feature    5005..5024 
                     /label="Restriction Enzyme"  
     misc_feature    5809..6481 
                     /label="ColE1"  
     misc_feature    6911..7705 
                     /label="Kan/Neo"  
     misc_feature    7740..8042 
                     /label="SV40 ori"  
     misc_feature    8128..8605 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4809..4815 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4809 
                     /label="SIK3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCCGCCCGT ATCGGCTACT ACGAGATCGA CCGCACCATC GGCAAGGGCA 
     1081 ACTTCGCGGT GGTCAAGCGG GCCACGCACC TCGTCACCAA GGCCAAGGTT GCTATCAAGA 
     1141 TCATAGATAA GACCCAGCTG GATGAAGAAA ACTTGAAGAA GATTTTCCGG GAAGTTCAAA 
     1201 TTATGAAGAT GCTTTGCCAC CCCCATATCA TCAGGCTCTA CCAGGTTATG GAGACAGAAC 
     1261 GGATGATTTA TCTGGTGACA GAATATGCTA GTGGAGGGGA AATATTTGAC CACCTGGTGG 
     1321 CCCATGGTAG AATGGCAGAA AAGGAGGCAC GTCGGAAGTT CAAACAGATC GTCACAGCTG 
     1381 TCTATTTTTG TCACTGTCGG AACATTGTTC ATCGTGATTT AAAAGCTGAA AATTTACTTC 
     1441 TGGATGCCAA TCTGAATATC AAAATAGCAG ATTTTGGTTT CAGTAACCTC TTCACTCCTG 
     1501 GGCAGCTGCT GAAGACCTGG TGTGGCAGCC CTCCCTATGC TGCACCTGAA CTCTTTGAAG 
     1561 GAAAAGAATA TGATGGGCCC AAAGTGGACA TCTGGAGCCT TGGAGTTGTC CTCTACGTGC 
     1621 TTGTGTGCGG TGCCCTGCCA TTTGATGGAA GCACACTGCA GAATCTGCGG GCCCGCGTGC 
     1681 TGAGTGGAAA GTTCCGCATC CCATTTTTTA TGTCCACAGA ATGTGAGCAT TTGATCCGCC 
     1741 ATATGTTGGT GTTAGATCCC AATAAGCGCC TCTCCATGGA GCAGATCTGC AAGCACAAGT 
     1801 GGATGAAGCT AGGGGACGCC GATCCCAACT TTGACAGGTT AATAGCTGAA TGCCAACAAC 
     1861 TAAAGGAAGA AAGACAGGTG GACCCCCTGA ATGAGGATGT CCTCTTGGCC ATGGAGGACA 
     1921 TGGGACTGGA CAAAGAACAG ACACTGCAGT CATTAAGATC AGATGCCTAT GATCACTATA 
     1981 GTGCAATCTA CAGCCTGCTG TGTGATCGAC ATAAGAGACA TAAAACCCTG CGTCTCGGAG 
     2041 CACTTCCTAG CATGCCCCGA GCCCTGGCCT TTCAAGCACC AGTCAATATC CAGGCGGAGC 
     2101 AGGCAGGTAC TGCTATGAAC ATCAGCGTTC CCCAGGTGCA GCTGATCAAC CCAGAGAACC 
     2161 AAATTGTGGA GCCGGATGGG ACACTGAATT TGGACAGTGA TGAGGGTGAA GAGCCTTCCC 
     2221 CTGAAGCATT GGTGCGCTAT TTGTCAATGA GGAGGCACAC AGTGGGTGTG GCTGACCCAC 
     2281 GCACGGAAGT TATGGAAGAT CTGCAGAAGC TCCTACCTGG CTTTCCTGGA GTCAACCCCC 
     2341 AGGCTCCATT CCTGCAGGTG GCCCCTAATG TGAACTTCAT GCACAACCTG TTGCCTATGC 
     2401 AAAACTTGCA ACCAACCGGG CAACTTGAGT ACAAGGAGCA GTCTCTCCTA CAGCCGCCCA 
     2461 CGCTACAGCT GTTGAATGGA ATGGGCCCCC TTGGCCGGAG GGCATCAGAT GGAGGAGCCA 
     2521 ACATCCAACT GCATGCCCAG CAGCTGCTGA AGCGCCCACG GGGACCCTCT CCGCTTGTCA 
     2581 CCATGACACC AGCAGTGCCA GCAGTTACCC CTGTGGACGA GGAGAGCTCA GACGGGGAGC 
     2641 CAGACCAGGA AGCTGTGCAG AGCTCTACCT ACAAGGACTC CAACACTCTG CACCTCCCTA 
     2701 CGGAGCGTTT CTCCCCTGTG CGCCGGTTCT CAGATGGGGC TGCGAGCATC CAGGCCTTCA 
     2761 AAGCTCACCT GGAAAAAATG GGCAACAACA GCAGCATCAA ACAGCTGCAG CAGGAGTGTG 
     2821 AGCAGCTGCA GAAGATGTAC GGGGGGCAGA TTGATGAAAG AACCCTGGAG AAGACCCAGC 
     2881 AGCAGCATAT GTTATACCAG CAGGAGCAGC ACCATCAAAT TCTCCAGCAA CAAATTCAAG 
     2941 ACTCTATCTG TCCTCCTCAG CCATCTCCAC CTCTTCAGGC TGCATGTGAA AATCAGCCAG 
     3001 CCCTCCTTAC CCATCAGCTC CAGAGGTTAA GGATTCAGCC TTCAAGCCCA CCCCCCAACC 
     3061 ACCCCAACAA CCATCTCTTC AGGCAGCCCA GTAATAGTCC TCCCCCCATG AGCAGTGCCA 
     3121 TGATCCAGCC TCACGGGGCT GCATCTTCTT CCCAGTTTCA AGGCTTACCT TCCCGCAGTG 
     3181 CAATCTTTCA GCAGCAACCT GAGAACTGTT CCTCTCCTCC CAACGTGGCA CTAACCTGCT 
     3241 TGGGTATGCA GCAGCCTGCT CAGTCACAGC AGGTCACCAT CCAAGTCCAA GAGCCTGTTG 
     3301 ACATGCTCAG CAACATGCCA GGCACAGCTG CAGGCTCCAG TGGGCGCGGC ATCTCCATCA 
     3361 GCCCCAGTGC TGGTCAGATG CAGATGCAGC ACCGTACCAA CCTGATGGCC ACCCTCAGCT 
     3421 ATGGGCACCG TCCCTTGTCC AAGCAGCTGA GTGCTGACAG TGCAGAGGCT CACAGCTTGA 
     3481 ACGTGAATCG GTTCTCCCCT GCTAACTACG ACCAGGCGCA TTTACACCCC CATCTGTTTT 
     3541 CGGACCAGTC CCGGGGTTCC CCCAGCAGCT ACAGCCCTTC AACAGGAGTG GGGTTCTCTC 
     3601 CAACCCAAGC CCTGAAAGTC CCTCCACTTG ACCAATTCCC CACCTTCCCT CCCAGTGCAC 
     3661 ATCAGCAGCC GCCACACTAT ACCACGTCGG CACTACAGCA GGCCCTGCTG TCTCCCACGC 
     3721 CGCCAGACTA TACAAGACAC CAGCAGGTAC CCCACATCCT TCAAGGACTG CTTTCTCCCC 
     3781 GGCATTCGCT CACCGGCCAC TCGGACATCC GGCTGCCCCC AACAGAGTTT GCACAGCTCA 
     3841 TTAAAAGGCA GCAGCAACAA CGGCAGCAGC AGCAGCAACA GCAGCAACAG CAAGAATACC 
     3901 AGGAACTGTT CAGGCACATG AACCAAGGGG ATGCGGGGAG TCTGGCTCCC AGCCTTGGGG 
     3961 GACAGAGCAT GACAGAGCGC CAGGCTTTAT CTTATCAAAA TGCTGACTCT TATCACCATC 
     4021 ACACCAGCCC CCAGCATCTG CTACAAATCA GGGCACAAGA ATGTGTCTCA CAGGCTTCCT 
     4081 CACCCACCCC GCCCCACGGG TATGCTCACC AGCCGGCACT GATGCATTCA GAGAGCATGG 
     4141 AGGAGGACTG CTCGTGTGAG GGGGCCAAGG ATGGCTTCCA AGACAGTAAG AGTTCAAGTA 
     4201 CATTGACCAA AGGTTGCCAT GACAGCCCTC TGCTCTTGAG TACCGGTGGA CCTGGGGACC 
     4261 CTGAATCTTT GCTAGGAACT GTGAGTCATG CCCAAGAATT GGGGATACAT CCCTATGGTC 
     4321 ATCAGCCAAC TGCTGCATTC AGTAAAAATA AGGTGCCCAG CAGAGAGCCT GTCATAGGGA 
     4381 ACTGCATGGA TAGAAGTTCT CCAGGACAAG CAGTGGAGCT GCCGGATCAC AATGGGCTCG 
     4441 GGTACCCAGC ACGCCCCTCC GTCCATGAGC ACCACAGGCC CCGGGCCCTC CAGAGACACC 
     4501 ACACGATCCA GAACAGCGAC GATGCTTATG TACAGCTGGA TAACTTGCCA GGAATGAGTC 
     4561 TCGTGGCTGG GAAAGCACTT AGCTCTGCCC GGATGTCGGA TGCAGTTCTC AGTCAGTCTT 
     4621 CGCTCATGGG CAGCCAGCAG TTTCAGGATG GGGAAAATGA GGAATGTGGG GCAAGCCTGG 
     4681 GAGGTCATGA GCACCCAGAC CTGAGTGATG GCAGCCAGCA TTTAAACTCC TCTTGCTATC 
     4741 CATCTACGTG TATTACAGAC ATTCTGCTCA GCTACAAGCA CCCCGAAGTC TCCTTCAGCA 
     4801 TGGAGCAGGC AGGCGTGACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     4861 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     4921 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     4981 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     5041 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     5101 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     5161 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     5221 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     5281 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     5341 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     5401 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     5461 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     5521 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     5581 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     5641 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     5701 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     5761 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     5821 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     5881 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     5941 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     6001 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     6061 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     6121 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     6181 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     6241 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     6301 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     6361 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     6421 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     6481 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     6541 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     6601 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     6661 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     6721 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     6781 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     6841 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     6901 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     6961 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     7021 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     7081 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     7141 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     7201 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     7261 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     7321 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     7381 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     7441 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     7501 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     7561 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     7621 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     7681 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     7741 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     7801 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     7861 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     7921 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7981 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     8041 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     8101 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     8161 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     8221 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     8281 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     8341 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     8401 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     8461 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     8521 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     8581 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     8641 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//