LOCUS       RC223399                7158 bp    DNA     circular  SYN 
DEFINITION  TRIM46 (Myc-DDK-tagged)-Human tripartite motif containing 46 (TRIM46)
ACCESSION   NM_025058
VERSION     NM_025058.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3315..3406 
                     /label="Myc-DDK"  
     misc_feature    3436..4021 
                     /label="PolyA signal"  
     misc_feature    3493..3512 
                     /label="Restriction Enzyme"  
     misc_feature    4297..4969 
                     /label="ColE1"  
     misc_feature    5399..6193 
                     /label="Kan/Neo"  
     misc_feature    6228..6530 
                     /label="SV40 ori"  
     misc_feature    6616..7093 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3297..3303 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3297 
                     /label="TRIM46"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCAGAGGGT GAGGATATGC AGACCTTCAC TTCCATCATG GACGCACTGG 
     1081 TCCGCATCAG TACCAGCATG AAGAACATGG AGAAGGAACT GCTGTGCCCA GTGTGTCAAG 
     1141 AGATGTACAA GCAGCCACTG GTGCTGCCCT GTACCCACAA CGTGTGCCAG GCCTGTGCCC 
     1201 GAGAGGTCTT GGGCCAGCAG GGCTACATAG GACATGGTGG GGACCCCAGC TCCGAGCCCA 
     1261 CCTCTCCTGC CTCCACCCCT TCCACCCGCA GCCCCCGCCT CTCCCGCAGA ACTCTCCCCA 
     1321 AGCCAGACCG CCTGGACCGG CTGCTTAAGT CAGGCTTTGG GACATACCCT GGGAGGAAGC 
     1381 GAGGTGCTTT GCACCCCCAA GTGATCATGT TCCCGTGCCC AGCCTGCCAA GGTGATGTGG 
     1441 AGCTTGGGGA GCGGGGTCTG GCAGGGCTTT TCCGGAACCT GACCCTGGAG CGTGTGGTGG 
     1501 AGCGGTACCG CCAGAGTGTG AGTGTGGGAG GTGCCATCCT GTGCCAGTTG TGCAAGCCCC 
     1561 CACCACTAGA GGCCACCAAG GGCTGCACAG AGTGCCGCGC CACCTTCTGC AATGAGTGCT 
     1621 TCAAGCTCTT CCACCCCTGG GGCACCCAGA AGGCCCAGCA TGAGCCCACC CTGCCTACCC 
     1681 TCTCCTTCCG ACCCAAGGGC CTTATGTGCC CAGACCACAA GGAAGAGGTG ACCCACTACT 
     1741 GCAAGACATG CCAACGCCTG GTATGTCAAC TCTGCCGGGT GCGGCGCACC CACAGCGGGC 
     1801 ACAAGATCAC ACCAGTGCTC AGTGCCTACC AGGCCCTCAA GGACAAGCTG ACAAAGAGCC 
     1861 TGACATACAT CCTGGGAAAC CAGGACACGG TACAGACCCA GATCTGTGAG CTGGAGGAGG 
     1921 CCGTGAGGCA CACCGAGGTG AGTGGTCAGC AGGCCAAGGA GGAGGTGTCG CAGCTGGTGC 
     1981 GGGGGCTGGG GGCTGTGCTG GAGGAGAAGC GGGCATCACT GCTTCAGGCC ATTGAAGAAT 
     2041 GCCAGCAGGA GCGGCTGGCC CGTCTCAGCG CCCAGATCCA GGAGCACCGG AGCCTGCTGG 
     2101 ATGGCTCAGG TCTGGTGGGC TATGCCCAGG AAGTACTTAA GGAAACAGAC CAGCCTTGCT 
     2161 TTGTGCAAGC CGCCAAGCAG CTGCACAACA GGATTGCCCG AGCCACTGAA GCCCTCCAGA 
     2221 CATTCCGGCC AGCTGCCAGC TCCTCCTTCC GCCATTGCCA GCTCGACGTG GGACGTGAGA 
     2281 TGAAGCTGCT GACAGAGCTT AACTTCCTGC GAGTGCCTGA GGCCCCCGTC ATTGACACCC 
     2341 AGCGCACCTT TGCCTATGAT CAGATCTTCC TGTGCTGGCG GCTGCCCCCC CATTCACCAC 
     2401 CTGCCTGGCA CTATACCGTT GAGTTCCGGC GCACGGATGT GCCTGCTCAG CCAGGCCCCA 
     2461 CCCGCTGGCA GCGGCGGGAG GAGGTGAGGG GCACCAGTGC CCTGCTTGAG AACCCCGACA 
     2521 CGGGCTCTGT GTATGTGCTG CGTGTCCGCG GCTGCAACAA GGCCGGCTAC GGCGAATACA 
     2581 GTGAAGATGT GCACCTGCAC ACGCCCCCGG CACCTGTCCT GCACTTCTTC CTCGATAGCC 
     2641 GCTGGGGCGC AAGCCGAGAG CGGCTGGCTA TCAGCAAGGA CCAGCGAGCA GTACGGAGTG 
     2701 TTCCAGGGCT GCCCCTGCTG CTGGCTGCTG ACCGGCTGCT GACCGGCTGC CACCTGAGTG 
     2761 TGGATGTGGT CCTGGGCGAC GTGGCTGTGA CCCAGGGCCG CAGCTACTGG GCCTGCGCCG 
     2821 TAGACCCAGC CTCCTACTTG GTCAAGGTGG GCGTCGGGCT GGAGAGCAAG CTTCAAGAAA 
     2881 GTTTCCAGGG TGCCCCCGAT GTGATCAGCC CCAGGTACGA CCCGGACAGC GGGCACGACA 
     2941 GCGGTGCCGA GGATGCCACA GTGGAGGCGT CGCCACCCTT CGCTTTCCTA ACCATTGGCA 
     3001 TGGGCAAGAT CCTGCTGGGG TCGGGGGCAA GCTCAAACGC AGGGCTGACA GGGAGGGATG 
     3061 GCCCCACAGC CGGCTGCACA GTGCCCCTGC CACCCCGCCT GGGCATCTGC CTGGACTATG 
     3121 AGCGGGGCCG GGTTTCCTTC CTGGATGCTG TTTCCTTCCG TGGGCTCTTG GAGTGCCCCC 
     3181 TGGACTGCTC AGGGCCTGTG TGCCCTGCCT TTTGCTTCAT CGGGGGTGGC GCAGTACAGC 
     3241 TCCAGGAGCC AGTGGGCACT AAGCCTGAGA GGAAAGTCAC CATTGGGGGC TTCGCCAAGC 
     3301 TGGACACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     3361 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     3421 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     3481 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     3541 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     3601 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     3661 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     3721 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     3781 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     3841 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     3901 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     3961 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     4021 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     4081 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     4141 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     4201 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     4261 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     4321 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     4381 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     4441 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     4501 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     4561 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     4621 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     4681 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     4741 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     4801 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     4861 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     4921 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     4981 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     5041 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     5101 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     5161 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     5221 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     5281 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     5341 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     5401 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     5461 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     5521 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     5581 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     5641 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     5701 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     5761 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     5821 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     5881 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     5941 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     6001 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     6061 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     6121 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     6181 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     6241 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     6301 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     6361 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     6421 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     6481 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     6541 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     6601 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     6661 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     6721 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     6781 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     6841 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     6901 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     6961 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     7021 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     7081 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     7141 AAATTTAACG CGAATTTT
//