LOCUS       RC223269                8619 bp    DNA     circular  SYN 
DEFINITION  WNK4 (Myc-DDK-tagged)-Human WNK lysine deficient protein kinase 4 (WNK4)
ACCESSION   NM_032387
VERSION     NM_032387.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4774..4865 
                     /label="Myc-DDK"  
     misc_feature    4895..5480 
                     /label="PolyA signal"  
     misc_feature    4952..4971 
                     /label="Restriction Enzyme"  
     misc_feature    5756..6428 
                     /label="ColE1"  
     misc_feature    6858..7652 
                     /label="Kan/Neo"  
     misc_feature    7687..7989 
                     /label="SV40 ori"  
     misc_feature    8075..8552 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4749..4756 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4749 
                     /label="WNK4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTTGGCATCC CCGGCCACGG AGACCACCGT CCTCATGTCC CAGACTGAGG 
     1081 CCGACCTGGC CCTGCGGCCC CCGCCTCCTC TTGGCACCGC GGGGCAGCCC CGCCTCGGGC 
     1141 CCCCTCCTCG CCGAGCGCGC CGCTTCTCCG GGAAGGCTGA GCCCCGGCCG CGCTCTTCTC 
     1201 GTCTCAGCCG CCGTAGCTCA GTCGACTTGG GGCTGCTGAG CTCTTGGTCC CTGCCAGCCT 
     1261 CACCCGCTCC GGACCCCCCC GATCCTCCGG ACTCCGCTGG TCCTGGCCCC GCGAGGAGCC 
     1321 CACCGCCTAG CTCCAAAGAA CCCCCCGAGG GCACGTGGAC CGAGGGAGCC CCTGTGAAGG 
     1381 CTGCGGAAGA CTCCGCGCGT CCCGAGCTCC CGGACTCTGC AGTGGGCCCG GGGTCCAGGG 
     1441 AGCCGCTAAG GGTCCCTGAA GCTGTGGCCC TAGAGCGGCG GCGGGAGCAG GAAGAAAAGG 
     1501 AGGACATGGA GACCCAGGCT GTGGCAACGT CCCCCGATGG CCGATACCTC AAGTTTGACA 
     1561 TCGAGATTGG ACGTGGCTCC TTCAAGACGG TGTATCGAGG GCTAGACACC GACACCACAG 
     1621 TGGAGGTGGC CTGGTGTGAG CTGCAGACTC GGAAACTGTC TAGAGCTGAG CGGCAGCGCT 
     1681 TCTCAGAGGA GGTGGAGATG CTCAAGGGGC TGCAGCACCC CAACATCGTC CGCTTCTATG 
     1741 ATTCGTGGAA GTCGGTGCTG AGGGGCCAGG TTTGCATCGT GCTGGTCACC GAACTCATGA 
     1801 CCTCGGGCAC GCTCAAGACG TACCTGAGGC GGTTCCGGGA GATGAAGCCG CGGGTCCTTC 
     1861 AGCGCTGGAG CCGCCAAATC CTGCGGGGAC TTCATTTCCT ACACTCCCGG GTTCCTCCCA 
     1921 TCCTGCACCG GGATCTCAAG TGCGACAATG TCTTTATCAC GGGACCTACT GGCTCTGTCA 
     1981 AAATCGGGGA CCTGGGCCTG GCCACGCTCA AGCGCGCCTC CTTTGCCAAG AGTGTCATCG 
     2041 GGACCCCGGA ATTCATGGCC CCCGAGATGT ACGAGGAAAA GTACGATGAG GCCGTGGACG 
     2101 TGTACGCGTT CGGCATGTGC ATGCTGGAGA TGGCCACCTC TGAGTACCCG TACTCCGAGT 
     2161 GCCAGAATGC CGCGCAAATC TACCGCAAGG TCACTTCGGG CAGAAAGCCG AACAGCTTCC 
     2221 ACAAGGTGAA GATACCCGAG GTGAAGGAGA TCATTGAAGG CTGCATCCGC ACGGATAAGA 
     2281 ACGAGAGGTT CACCATCCAG GACCTCCTGG CCCACGCCTT CTTCCGCGAG GAGCGCGGTG 
     2341 TGCACGTGGA ACTAGCGGAG GAGGACGACG GCGAGAAGCC GGGCCTCAAG CTCTGGCTGC 
     2401 GCATGGAGGA CGCGCGGCGC GGGGGGCGCC CACGGGACAA CCAGGCCATC GAGTTCCTGT 
     2461 TCCAGCTGGG CCGGGACGCG GCCGAGGAGG TGGCACAGGA GATGGTGGCT CTGGGCTTGG 
     2521 TCTGTGAAGC CGATTACCAG CCAGTGGCCC GTGCAGTACG TGAACGGGTT GCTGCCATCC 
     2581 AGCGAAAGCG TGAGAAGCTG CGTAAAGCAA GGGAATTGGA GGCACTCCCA CCAGAGCCAG 
     2641 GACCTCCACC AGCAACTGTG CCCATGGCCC CCGGTCCCCC CAGTGTCTTC CCCCCTGAGC 
     2701 CTGAGGAGCC AGAGGCAGAC CAGCACCAGC CCTTCCTTTT CCGCCACGCC AGCTACTCAT 
     2761 CTACCACTTC GGATTGCGAG ACTGATGGCT ACCTCAGCTC CTCCGGCTTC CTGGATGCCT 
     2821 CAGACCCTGC CCTTCAGCCC CCTGGGGGGG TGCCATCCAG CCTGGCTGAG TCCCATCTCT 
     2881 GCCTGCCCTC GGCTTTTGCC CTATCCATTC CACGTTCTGG CCCTGGAAGT GACTTTTCCC 
     2941 CCGGGGACAG CTATGCCTCA GATGCAGCTT CAGGCCTTAG CGATGTGGGA GAAGGGATGG 
     3001 GACAAATGAG GAGACCCCCA GGGAGGAATC TCCGGCGCAG ACCCCGATCC CGGCTGCGGG 
     3061 TCACTAGTGT CTCAGACCAG AATGACAGAG TGGTTGAGTG CCAGCTACAG ACCCATAACA 
     3121 GCAAGATGGT GACCTTCCGA TTTGATCTGG ATGGGGACAG CCCGGAAGAG ATTGCAGCTG 
     3181 CCATGGTATA TAACGAGTTC ATTCTGCCTT CGGAGCGAGA TGGATTTCTC AGACGGATTC 
     3241 GGGAGATTAT CCAGCGAGTG GAGACCCTGT TGAAGAGAGA CACTGGCCCC ATGGAGGCTG 
     3301 CTGAAGACAC CCTAAGCCCC CAGGAGGAGC CAGCACCATT ACCTGCCCTG CCCGTCCCCC 
     3361 TCCCAGACCC ATCCAATGAA GAGCTCCAGA GCAGCACCTC CCTGGAGCAC AGGAGCTGGA 
     3421 CAGCCTTCTC CACCTCCTCA TCTTCTCCTG GAACTCCTTT GTCTCCTGGA AACCCATTTT 
     3481 CCCCTGGAAC CCCCATTTCC CCAGGTCCCA TCTTCCCCAT CACTTCTCCC CCATGTCATC 
     3541 CCAGCCCCTC CCCATTCTCC CCCATTTCTT CCCAGGTCTC CTCAAATCCC TCTCCACACC 
     3601 CCACCAGCTC TCCACTTCCA TTCTCCTCCA GCACACCCGA GTTTCCGGTC CCACTCTCTC 
     3661 AGTGTCCCTG GAGTTCTCTC CCCACGACTT CTCCACCTAC GTTCTCTCCC ACTTGTTCTC 
     3721 AGGTCACTCT TAGTTCCCCT TTCTTTCCTC CGTGCCCCTC CACTTCTTCC TTCCCCTCCA 
     3781 CCACAGCAGC CCCTCTCCTT TCTCTGGCTA GTGCCTTCTC ACTGGCTGTG ATGACTGTGG 
     3841 CCCAGTCCCT GCTGTCCCCC TCACCTGGGC TCCTTTCCCA GTCTCCTCCA GCCCCTCCTA 
     3901 GTCCCCTCCC TAGCCTGCCC CTTCCCCCTC CCGTTGCTCC TGGTGGCCAG GAAAGCCCTT 
     3961 CACCCCACAC AGCTGAGGTG GAGAGTGAGG CCTCACCACC TCCTGCTCGG CCCCTCCCAG 
     4021 GGGAAGCCAG GCTGGCGCCC ATCTCTGAAG AGGGAAAGCC GCAGCTTGTT GGGCGTTTCC 
     4081 AAGTGACTTC ATCCAAGGAA CCGGCTGAGC CTCTTCCCTT GCAGCCAACA TCCCCCACTC 
     4141 TCTCTGGTTC TCCAAAACCT TCAACCCCTC AGCTCACTTC AGAGAGCTCA GATACAGAGG 
     4201 ACAGTGCTGG AGGCGGGCCA GAGACCAGGG AAGCTCTGGC TGAGAGCGAC CGTGCAGCTG 
     4261 AGGGTCTGGG GGCTGGAGTT GAGGAGGAAG GAGATGATGG GAAGGAACCC CAAGTTGGGG 
     4321 GCAGCCCCCA ACCCCTGAGC CATCCCAGCC CAGTGTGGAT GAACTACTCC TACAGCAGCC 
     4381 TGTGTTTGAG CAGCGAGGAG TCAGAAAGCA GTGGGGAAGA TGAGGAGTTC TGGGCTGAGC 
     4441 TGCAGAGTCT TCGGCAGAAG CACTTGTCAG AGGTGGAAAC ACTACAGACA CTACAGAAAA 
     4501 AAGAAATTGA AGATTTGTAC AGCCGGCTGG GGAAGCAGCC CCCACCGGGT ATTGTGGCCC 
     4561 CAGCTGCTAT GCTGTCCAGC CGCCAGCGCC GCCTCTCCAA GGGCAGCTTC CCCACCTCCC 
     4621 GCCGCAACAG CCTACAGCGC TCTGAGCCCC CAGGCCCTGG CATCATGCGA AGGAACTCTC 
     4681 TGAGTGGCAG CAGCACCGGC TCCCAGGAGC AGCGGGCAAG CAAGGGGGTG ACATTCGCCG 
     4741 GGGATGTTGG CAGGATGAGC GGACCGACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     4801 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     4861 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     4921 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     4981 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     5041 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     5101 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     5161 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     5221 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     5281 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     5341 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     5401 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     5461 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     5521 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     5581 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     5641 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     5701 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     5761 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     5821 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     5881 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     5941 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     6001 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     6061 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     6121 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     6181 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     6241 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     6301 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     6361 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     6421 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     6481 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     6541 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     6601 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     6661 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     6721 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     6781 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     6841 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     6901 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     6961 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     7021 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     7081 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     7141 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     7201 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     7261 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     7321 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     7381 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     7441 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     7501 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     7561 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     7621 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     7681 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     7741 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     7801 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     7861 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7921 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7981 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     8041 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     8101 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     8161 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     8221 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     8281 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     8341 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     8401 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     8461 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     8521 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     8581 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//